:: Volume 18, Issue 1 (1-2014) ::
IBJ 2014, 18(1): 16-22 Back to browse issues page
Antioxidant Role of Oleuropein on Midbrain and Dopaminergic Neurons of Substantia Nigra in Aged Rats
Maryam Sarbishegi , Fereshteh Mehraein , Mansooreh Soleimani
Abstract:  
Background: Oleuropein is a phenolic compound which is present in the olive leaf extract. The purpose of the present study was to investigate the neuroprotective effect of oleuropein as an antioxidant agent on the substantia nigra in aged rats. Methods: Twenty 18-month-old Wistar rats (450-550 g) were randomly divided into control and experimental groups. The experimental group received a daily single dose of 50 mg/kg of oleuropein by oral gavage for 6 months. The control group received only distilled water. All rats were sacrificed two hours after the last gavage and the brains were removed and midbrains were cut. One part of the midbrains were homogenized and centrifuged. The tissue supernatant was assayed for lipid peroxidation (LPO) and antioxidant enzyme activities. The other part of midbrains fixed and embedded in paraffin, then processed for Nissl and immunohistochemistry (IHC) staining. Data was analyzed using SPSS by t-test. Differences were considered significant for P<0.05. Results: The level of LPO in midbrain of the rats was decreased significantly in the experimental group, but superoxide dismutase, catalase and glutathione peroxidase activities were increased in experimental group compared to control group (P<0.05). Morphometric analyses showed significantly that the experimental group had more neurons in substantia nigra pars compacta (SNc) either in Nissl or IHC staining when compared to control (P<0.05). Conclusion: The results of the present study indicate that treatment of the old rats with oleuropein reduces the oxidative damage in SNc by increasing the antioxidant enzyme activities.
Keywords: Oleuropein, Aging, Dopaminergic neurons, Substantia nigra
Full-Text [PDF 644 kb]      
Type of Study: Full Length | Subject: Related Fields
Send email to the article author

Add your comments about this article
Your username or Email:

Write the security code in the box >



DOI: 10.6091/ibj.1274.2013
PMID: 24375158
PMCID: PMC3892135


XML     Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Sarbishegi M, Mehraein F, Soleimani M. Antioxidant Role of Oleuropein on Midbrain and Dopaminergic Neurons of Substantia Nigra in Aged Rats. IBJ. 2014; 18 (1) :16-22
URL: http://ibj.pasteur.ac.ir/article-1-1083-en.html
Volume 18, Issue 1 (1-2014) Back to browse issues page
Persian site map - English site map - Created in 0.179 seconds with 869 queries by yektaweb 3525