Volume 17, Issue 4 (10-2013)                   ibj 2013, 17(4): 187-193 | Back to browse issues page


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Yadegari M, Orazizadeh M, Hashemitabar M, Khodadadi A. Protective Effects of Interleukin-4 on Tissue Destruction and Morphological Changes of Bovine Nasal Chondrocytes in vitro. ibj. 2013; 17 (4) :187-193
URL: http://ibj.pasteur.ac.ir/article-1-1032-en.html
Abstract:  
Background: Previous studies have shown that some cytokines have protective effects on cartilage in joint diseases. In the current study, effects of IL-4 against morphological changes and tissue degradation induced by IL-1α on bovine nasal cartilage (BNC) explants were investigated. Methods: Fresh BNC samples were prepared from a slaughterhouse under sterile conditions. BNC explants culture was treated with both IL-lα (10 ng/ml) and IL-4 (50 ng/ml) at the same time for 28 days. The morphological characteristics of explants were assessed by using histology techniques and invert microscopy. Matrix metalloproteinase-1 (MMP-1) production was assessed within different days by using Western blotting. Results: IL-lα induced prominent cartilage morphology degradation. The pro and active form of MMP-1 band substantially increased at day 21 of culture. In the presence of both IL-lα and IL-4, chondrocytes preserved their ordinary normal phenotype with intact extracellular matrix. In addition, a significant reduction in pro-MMP-1and inhibition of active MMP-1 was seen. Conclusion: In conclusion, IL-4 could be regarded as a potential candidate in cartilage protecting against the degradation changes of IL-lα. It seems that the preservation effect of IL-4 is associated with significant reduction of MMP-1.
Type of Study: Full Length | Subject: Related Fields

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