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Biomedical Journal
Volume 28, Issue 1 (1-2024)
IBJ 2024, 28(1): 53-58 |
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Mehrzad N, Zamani M S, Rahimi A A, Shamaei M, Karimipoor M. Methylation Status of miR-34a and miR-126 in Non-Small Cell Lung Cancer (NSCLC) Tumor Tissues. IBJ 2024; 28 (1) :53-58
URL: http://ibj.pasteur.ac.ir/article-1-3845-en.html
URL: http://ibj.pasteur.ac.ir/article-1-3845-en.html
Nazanin Mehrzad 
, Mohammad Saber Zamani , Amir Abbass Rahimi , Masoud Shamaei , Morteza Karimipoor *
, Mohammad Saber Zamani , Amir Abbass Rahimi , Masoud Shamaei , Morteza Karimipoor *
Abstract:
Background: MiR-34a and miR-126 mainly act as tumor suppressors and are often downregulated in various cancers, including non-small cell lung cancer (NSCLC). We aimed to determine the methylation status of miR-34a and miR-126 in NSCLC patients.
Methods: The current study included 63 paraffin-embedded NSCLC and paired adjacent normal tissues. After DNA extraction and bisulfite treatment, the methylation status of miR-34a and miR-126 were evaluated using the MSP method.
Results: There was no statistically significant difference between tumor and normal tissues regarding the methylation status of miR-34a and miR-126
(p > 0.05). Moreover, we found no significant correlation between the methylation status of miR-34a and miR-126 with patients’ demographic parameters, including gender, age, and pathology subtype (p > 0.05).
Conclusion: Considering the low expression of mir-126 and mir-34 in NSCLC, more sensitive methods are recommended to be exploited for detecting the level of methylation or underlying mechanisms other than promoter hypermethylation in silencing these genes in NSCLC.
Methods: The current study included 63 paraffin-embedded NSCLC and paired adjacent normal tissues. After DNA extraction and bisulfite treatment, the methylation status of miR-34a and miR-126 were evaluated using the MSP method.
Results: There was no statistically significant difference between tumor and normal tissues regarding the methylation status of miR-34a and miR-126
(p > 0.05). Moreover, we found no significant correlation between the methylation status of miR-34a and miR-126 with patients’ demographic parameters, including gender, age, and pathology subtype (p > 0.05).
Conclusion: Considering the low expression of mir-126 and mir-34 in NSCLC, more sensitive methods are recommended to be exploited for detecting the level of methylation or underlying mechanisms other than promoter hypermethylation in silencing these genes in NSCLC.
Type of Study: Short Communication |
Subject:
Molecular Genetics & Genomics
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