Volume 27, Issue 1 (1-2023)                   IBJ 2023, 27(1): 58-65 | Back to browse issues page

PMID: 36624688


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Abstract:  
Background: Lysozyme is a part of human and animal noncellular immunity. The regulation of its activity by hormones is poorly studied. The aim of this study was to test the in vitro activity of lysozyme in the presence of catecholamines, natriuretic hormones, and estradiol (E2).
Methods: Hormones were incubated with lysozyme, and the activity of lysozome was further determined using a test culture of Micrococcus luteus in the early exponential growth stage. The activity of lysozyme was assessed based on the rate of change in the OD of the test culture. Molecular docking was performed using SwissDock server (http://www.swissdock.ch/docking), and molecular structures were further analyzed and visualized in the UCSF Chimera 1.15rc software.
Results: According to the results, epinephrine and norepinephrine increased lysozyme activity up to 180% compared to the hormone-free enzyme. Changing the pH of the medium from 6.3 to 5.5, increased the lysozyme activity in the presence of E2 up to 150-200 %. The results also showed that exposure to hormones could modify lysozyme activity, and this effect depends on the temperature and pH value. The molecular docking revealed a decrease in the activation energy of the active site of enzyme during the interaction of catecholamines with the amino acid residues, asp52 and glu35 of the active site.
Conclusion: Our findings demonstrate an additional mechanism for the involvement of lysozyme in humoral regulation of nonspecific immunity with respect to human pathogenic microflora and bacterial skin commensals by direct modulation of its activity using human hormones.

References
1. Dumoulin M, Johnson RJK, Bellotti V, Dobson CM. Human Lysozyme. Protein misfolding, aggregation, and conformational diseases 2007; 6: 285-308. [DOI:10.1007/978-0-387-36534-3_14]
2. Hankiewicz J, Swierczek E. Lysozyme in human body fluids. Clinica chimica acta 1974; 57: 205-209. [DOI:10.1016/0009-8981(74)90398-2]
3. Ogawa H, Miyazaki H, Kimura M. Isolation and characterization of human skin lysozyme. The journal of investigative dermatology 1971; 57(2): 111-116. [DOI:10.1111/1523-1747.ep12349624]
4. Klenha J, Krs V. Lysozyme in mouse and human skin. Journal of investigative dermatology 1967; 49: 396-399. [DOI:10.1038/jid.1967.155]
5. Papini M, Simonetti S, Franceschini S, Binazzi M. Serum and skin lysozyme activity in several skin disorders. Archives of dermatological research 1983; 275: 67-68. [DOI:10.1007/BF00516559]
6. Davis KM, Weiser JN. Modifications to the peptidoglycan backbone help bacteria to establish infection. Infection and immunity 2011; 79(2b): 562-570. [DOI:10.1128/IAI.00651-10]
7. Ragland SA, Criss AK. From bacterial killing to immune modulation: recent insights into the functions of lysozyme. PLOS pathogens 2017; 13(9): e1006512. [DOI:10.1371/journal.ppat.1006512]
8. Gannesen AV, Lesouhaitier O, Netrusov AI, Plakunov VK, Feuilloley MGJ. Regulation of Formation of Monospecies and Binary Biofilms by Human Skin Microbiota Components, Staphylococcus epidermidis and Staphylococcus aureus, by Human Natriuretic Peptides. Microbiology 2018; 87: 597-609. [DOI:10.1134/S0026261718050090]
9. Cambronel M, Tortuel D, Biaggini K, Maillot O, Taupin L, Réhel K, Rincé I, Muller C , Hardouin J, Feuilloley M, Rodrigues S, Connil N. Epinephrine affects motility, and increases adhesion, biofilm and virulence of Pseudomonas aeruginosa H103. Scientific reports 2019; 9: 20203. [DOI:10.1038/s41598-019-56666-7]
10. Cambronel M, Nilly F, Mesguida O, Boukerb AM, Racine PJ, Baccouri O, Borrel V, Martel J, Fécamp F, Knowlton R, Zimmermann K, Domann E, Rodrigues S, Feuilloley M, Connil N. Influence of catecholamines (epinephrine/norepinephrine) on biofilm formation and adhesion in pathogenic and probiotic strains of Enterococcus faecalis. Frontiers in microbiology 2020; 11: 1501. [DOI:10.3389/fmicb.2020.01501]
11. Krupyanskii YF, Abdulnasyrov EG, Loiko NG, Stepanov AS, Tereshkina KB, EL-rejistan GI. Possible mechanisms of the influence of hexylresorcinol on the structure-dynamic and functional properties of lysozyme protein. Russian journal of physical chemistry B 2012; 6: 301-314. [DOI:10.1134/S1990793112020078]
12. Gorin G, Wang SF, Papapavlou L. Assay of lysozyme by its lytic action on M. lysodeikticus cells. Analytical biochemistry 1971; 39: 113-116. [DOI:10.1016/0003-2697(71)90467-2]
13. Grosdidier A, Zoete V, Michielin O. Docking of small molecules into protein active sites with a multiobjective evolutionary optimization. Proteins 2007; 67: 1010-1025. [DOI:10.1002/prot.21367]
14. Nusrat S, Masroor A, Zaman M, Siddiqi MK, Ajmal MR, Zaidi N, Abdelhameed AS, Khan RH. Interaction of catecholamine precursor L-Dopa with lysozyme: a biophysical insight. International journal of biological macromolecules 2018; 109: 1132-1139. [DOI:10.1016/j.ijbiomac.2017.11.107]
15. Bergamo A, Gerdol M, Pallavicini A, Greco S, Schepens I, Hamelin R, Armand F, Dyson PJ, Sava G. Lysozyme-Induced transcriptional regulation of TNF- α pathway genes in cells of the monocyte lineage. International journal of molecular sciences 2019; 20(21): 5502. [DOI:10.3390/ijms20215502]
16. Hawiger J. Purification and properties of lysozyme produced by Staphylococcus aureus. Journal of bacteriology 1968; 95(2): 376-384. [DOI:10.1128/jb.95.2.376-384.1968]
17. Callewaert L, Van Herreweghe JM, Vanderkelen L, Leysen S, Voet A, Michiels CW. Guards of the great wall: bacterial lysozyme inhibitors. Trends microbiology 2012; 20(10): 501-510. [DOI:10.1016/j.tim.2012.06.005]
18. Kaiser E. Inhibition and activation of lysozyme. Nature 1953; 4353: 607-608. [DOI:10.1038/171607a0]
19. Manwaring WH. Biotin activation of lysozyme. California and western medicine 1944; 61(1): 8-9.
20. Roberts RM, Bazer FW, Baldwin N, Pollard WE. Progesterone induction of lysozyme and peptidase activities in the porcine uretrus. Archives of biochemistry and biophysics 1976; 177: 499-507. [DOI:10.1016/0003-9861(76)90461-6]
21. Small BS, Bilodeau AL. Effects of cortisol and stress on channel catfish (Ictalurus punctatus) pathogen susceptibility and lysozyme activity following exposure to Edwardsiella ictaluri. General and comparative endocrinology 2005; 142: 256-262. [DOI:10.1016/j.ygcen.2004.12.004]
22. Smith SJ, Saggerson ED. Regulation of pyruvate dehydrogenase activity in rat epididymal fat-pads and isolated adipocytes by adrenaline. Biochemical journal 1978; 174: 119-130. [DOI:10.1042/bj1740119]
23. Demers NE, Bayne CJ. The immediate effects of stress on hormones and plasma lysozyme in rainbow trout. Developmental and Comparative immunology 1997; 21(4): 363-373. [DOI:10.1016/S0145-305X(97)00009-8]
24. Gosain A, Gamelli RL, DiPietro LA. Norepinephrine-mediated suppression of phagocytosis by wound neutrophils. Journal of surgical research 2009; 152(2): 311-318. [DOI:10.1016/j.jss.2008.05.001]
25. Nicholls AJ, Wen SW, Hall P, Hickey MJ, Wong CHY. Activation of the sympathetic nervous system modulates neutrophil function. Journal of leukocyte biology 2018; 103: 295-309. [DOI:10.1002/JLB.3MA0517-194RR]
26. Lambers H, Piessens S, Bloem A, Pronk H, Finkel P. Natural skin surface pH is on average below 5, which is beneficial for its resident flora. International journal of cosmetic science 2006; 28(5): 359-370. [DOI:10.1111/j.1467-2494.2006.00344.x]
27. Eggert-Kruse W, Botz I, Pohl S, Rohr G, Strowitzki T. Antimicrobial activity of human cervical mucus. Human reproduction 2000; 15(4): 778-784. [DOI:10.1093/humrep/15.4.778]
28. Anderson LS, Reynolds MB, Rivara, KR, Miller LS, Simon SI. A mouse model to assess innate immune response to Staphylococcus aureus infection. Journal of visualized experiments 2019; 144: 10. [DOI:10.3791/59015-v]

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