Volume 28, Issue 2 And 3 (3-2024)                   IBJ 2024, 28(2 And 3): 131-138 | Back to browse issues page

Ethics code: IR.PII.REC.1399.045


XML Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Teflischi Gharavi A, Niknejad A, Irian S, Rahimi A, Salimi M. Polyethylene Glycol-Mediated Exosome Isolation: A Method for Exosomal RNA Analysis. IBJ 2024; 28 (2 and 3) :131-138
URL: http://ibj.pasteur.ac.ir/article-1-4129-en.html
Abstract:  
Background: ExoRNAs offer valuable insights into their cellular origin. ExoRNA studies were faced with challenges in obtaining sufficient amounts of high-quality RNA. Herein, we aimed to compare three traditional exosome isolation methods to introduce an appropriate strategy to extract RNA from cancer-derived exosomes for further RNA analysis.
Methods: Exosomes were isolated through ultracentrifugation, precipitation kit, and size exclusion column chromatography, and then characterized by DLS and TEM, followed by extracting total RNA. The quality and quantity of the extracted RNAs were assessed by a NanoDrop and 2.5% agarose gel electrophoresis.
Results: Extracted exosomes displayed a similar range of size and morphology. We found that PEG-precipitation method resulted in a higher RNA yield with a 260/280 ratio of 1.9. The obtained exoRNA appeared as a smear in the agarose gel, indicative of small exoRNAs.
Conclusion: We provide researchers a suitable approach to isolate exosomes based on yield and purity of exoRNA.

 

Add your comments about this article : Your username or Email:
CAPTCHA

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2024 CC BY-NC 4.0 | Iranian Biomedical Journal

Designed & Developed by : Yektaweb