Pasteur Institute of Iran
Iranian Biomedical Journal
1028-852X
2008-823X
7
4
2003
10
1
Supportive Effects of Human Embryonic Fibroblast Cell Lines on Growth and Proliferation of EBV-Transformed Lymphoblastoid Cells
147
153
EN
Saeed
Rajabalian
N
Zahra
Samadi Bahrami
N
Vahideh
Farahat
N
Fazel
Shokri
fazshok@yahoo.com
Y
Human diploid fibroblast cells produce a spectrum of necessary growth factors and extracellular matrix (ECM) components essential for growth and proliferation of a variety of other cell types. In this study, the effect of five human embryonic fibroblast cell lines, isolated from liver, lung, skin and foreskin tissues, was investigated. A coculture system analyse was employed to cloning efficiency (CE) and DNA synthesis of a human Epstein-Barr virus (EBV)-transformed lymphoblastoid cell line (LCL) in long- and short-term cultures. The fibroblast cells were used as feeder layer after treatment with mitomycin C. Optimal density of the feeder cells induced 10 to 43 times higher CE than cultures supplemented with conditioned media (CM) or cultures without a feeder layer. The stimulatory effect of the feeder cells was partly associated to their tissue origin, with the lung and liver fibroblasts being the most and least effective feeder cells, respectively. Short-term cultures of LCL cells with feeder cells or their CM resulted in a marginal increase in DNA synthesis and proliferation as evidenced by the index of ³H-thymidine incorporation. Our results demonstrated supportive effects of feeder cells on the LCL growth, which can not be replaced by their CM. These supportive effects were partly associated with cell density and tissue origin of the feeder cells
Embryo, Fibroblast, Feeder cells, EBV-transformed B-cells, Cloning efficiency
http://ibj.pasteur.ac.ir/article-1-521-en.html
http://ibj.pasteur.ac.ir/article-1-521-en.pdf
Pasteur Institute of Iran
Iranian Biomedical Journal
1028-852X
2008-823X
7
4
2003
10
1
Presence of Immunoglobulins on the Surface of Lesion-Derived Amastigotes of Leishmania major As a Tool for Isolation of Amastigote Stage from BALB/c Mice
155
160
EN
Ali
Mirjalili
N
Mohammad H
Alimohammadian
mhalimoh@institute.pasteur.ac.ir
Y
Rasoul
Madani
N
The onset of infections by Leishmania parasites mainly caused by amastigote growth inside the macrophages. One of the important properties of lesion-derived amastigote is thought to be the attachment of various host proteins including immunoglobulins on the surface of amastigote. In this study, the presence of immunoglobulins on the surface of lesion-derived amastigotes was detected by Western blotting using three different peroxidase conjugated anti-heavy chain antibodies and peroxidase conjugated anti-mouse IgG antibody. Then, a new technique was developed for isolation of lesion-derived amastigotes. This technique simply consists of a microbiological plate covered by rabbit anti-mouse immunoglobulins. Overnight incubation of the infected cell suspension isolated from mice lesion on such plate at 4°C, was followed by gentle washing and isolation of the amastigotes. The results showed that the surface of amastigote has covered with different amount of immunoglobulins such as IgG, IgM, and IgA detected by pixel analysis software. With this technique, which was comparable with other techniques, pure amastigote was isolated. This is a simple and reliable method for isolation of real amastigotes from lesion of the infected BALB/c mice
Leishmania major, Lesion-derived amastigote, Immunoglobulins , BALB/c mice
http://ibj.pasteur.ac.ir/article-1-522-en.html
http://ibj.pasteur.ac.ir/article-1-522-en.pdf
Pasteur Institute of Iran
Iranian Biomedical Journal
1028-852X
2008-823X
7
4
2003
10
1
A Study on the Effects of Modulation of Intracellular Calcium on Excisional Wound Healing in Rabbit
161
166
EN
Mohammad Hassan
Pipelzadeh
Y
Mohammad Reza
Pipelzadeh
N
Popak
Husseinzadeh
N
An in vitro study on the role of intracellular calcium ions in healing of excisional wound in rabbit was undertaken. We employed two drugs namely, glibenclamide and nitroglycerin that are topically applied in vivo to modulate the activity of intracellular calcium. Our model consisted of a 15 ´ 15 mm excisional wound. Seven groups of New Zealand rabbits were used. The first three groups served as untreated, Vaseline- and lubricating jell vehicle-treated as controls. The remaining groups received topically 0.5 g of nitroglycerine (2 % in Vaseline base) or glibenclamide (1, 2 and 4% in lubricating jell) on the day of excision and continued for 11 days. Using wound surface area measurement, complemented with measurement of the breaking strength and histological assessment, the results showed that inhibition of intracellular calcium ion had a favorable effect on wound healing. The mean wound half-lives and breaking strength were significant and concentration dependently reduced in glibenclamide-treated group. In contrast, in nitroglycerin-treated group the rate of wound healing and breaking strength were increased relative to untreated control and Vaseline treated groups. Histological findings revealed more organized collagen fibers and angiogenesis in nitroglycerin-treated wounds. The present study demonstrated that intracellular calcium ion has an important role on the overall process of wound healing. This information may be utilized in further studies to assess its role in healing of human wounds
Excisional wound, Nitroglycerin, Glibenclamide, Wound surface area, Tensile strength
http://ibj.pasteur.ac.ir/article-1-523-en.html
http://ibj.pasteur.ac.ir/article-1-523-en.pdf
Pasteur Institute of Iran
Iranian Biomedical Journal
1028-852X
2008-823X
7
4
2003
10
1
Changes in the Level of Mitochondrial and Cytosolic Aspartate Aminotransferase Activities in Aluminium Intoxified Rat
167
171
EN
Ali Asghar
Moshtaghie
Moshtaghie@PharmD.mui.ac.ir
Y
Iraj
Javadi
N
Golamreza
Feghhi
N
The activity of aspartate aminotransferase (AST) in human serum has been widely determined as a diagnostic aid in liver disease. In this study, the effect of aluminium on AST isoenzymes in relation to aluminium intoxified patients has been investigated. Using gel filtration chromatography technique with Sephacryl S-300, mitochondrial aminotransferase (m-AST) and cytosolic aminotransferase (c-AST) fractions were separated from rat serum and liver homogenate. The c-AST fraction was eluted with higher mobility than m-AST iso-enzyme. Daily administration of aluminium (1 and 5 mg/kg body weight) for 30 days increased total serum activity of AST by 19% and 72%, respectively. Daily administration of aluminium (10 and 20 mg/kg body weight) for 30 days was also studied. The percentage of elevations was 114% and 86% in comparison to the controls. Following aluminium administration for 45 days, the enzyme activity was elevated to 20% and 60% in comparison to the controls, and administration for 60 days resulted elevation of 35% and 79%. The serum enzyme activity was mostly due to the mitochondrial fraction of AST that was a time and dose dependent
Mitochondria, Cytosolic, Activity of aspartate aminotransferase (AST), Aluminium, Intoxified
http://ibj.pasteur.ac.ir/article-1-524-en.html
http://ibj.pasteur.ac.ir/article-1-524-en.pdf
Pasteur Institute of Iran
Iranian Biomedical Journal
1028-852X
2008-823X
7
4
2003
10
1
The Effect of Quinapril on the Aortic Contractile Response of Streptozotocin-Diabetic Rats
173
177
EN
Farshad
Roghani Dehkordi
N
Mehrdad
Roghani
mehjour@yahoo.com
Y
Tourandokht
Baluchnejad Mojarad
N
Angiotensin-converting enzyme (ACE) inhibitors appear to correct many of the abnormalities associated with the vascular dysfunction found in diabetic patients. In this respect, quinapril is a unique ACE inhibitor with multiple protective effects. The present study was carried out to investigate the effect of intraperitoneal administration of quinapril on the aortic reactivity of streptozotocin (STZ)-diabetic rats. For this purpose, male Wistar rats received one injection of streptozotocin (STZ), 60 mg/kg, to induce diabetes. Three days after STZ injection, rats were treated with quinapril (2 mg/kg/day) for 4 weeks, after that aortic reactivity to vasoactive agents were compared with those of untreated diabetic rats or non-diabetic control rats. For this purpose, contractile response to phenylephrine (PE) was obtained from aortic rings. Concentration-response curves from quinapril-treated diabetic rats to PE in the presence and absence of endothelium were attenuated as compared to vehicle-treated diabetics. Therefore, the 4-week treatment of diabetic rats with quinapril could prevent the functional changes in vascular reactivity in diabetic rats
Quinapril, Aortic reactivity, Diabetes mellitus, STZ, Rat
http://ibj.pasteur.ac.ir/article-1-525-en.html
http://ibj.pasteur.ac.ir/article-1-525-en.pdf
Pasteur Institute of Iran
Iranian Biomedical Journal
1028-852X
2008-823X
7
4
2003
10
1
Effect of Vanadyl Sulfate on Fructose-Induced Insulin Resistance Rat
179
182
EN
Mehdi
Harati
mharati@medinews.com
Y
Mohsen
Ani
N
Manoochehr
Messripour
N
Insulin resistance syndrome, also referred to as the metabolic syndrome or syndrome X, refers to a constellation of common metabolic and cardiovascular disorders (e.g. obesity, type 2 diabetes mellitus, hypertension, and dyslipidemia), which are all cardiovascular risk factors. Insulin resistance can be induced by fructose-rich diet in rats. We investigated the effect of vanadyl sulfate (0.2 mg/ml in drinking water for 7 days) on glucose, triglyceride, and plasma insulin levels in male Wistar rats that were fed with fructose-rich diets. Control rats were fed with standard chow for 7 days. The animals were divided into three groups: fructose-fed rats, fructose fed-vanadyl sulfate treated rats, and control rats. Fasting plasma glucose levels of the three groups were comparable (p>0.05). Fasting plasma insulin increased in the fructose-fed rats (190 ± 6.3 pM vs. control rats 83.06 ± 3.3 pM, p<0.001), likewise, plasma triglyceride significantly increased in fructose-fed rats (394.0 ± 25.8 vs. control rats 98.63 ± 6.7, p<0001). Vanadyl sulfate treatment prevented the increase in plasma insulin levels in the fructose fed-vanadyl treated rats (78.9 ± 5.1 pM vs. fructose fed-groups, p<0.001). Also vanadyl sulfate treatment significantly decreased plasma triglyceride levels (116.43 ± 6.7 vs. fructose-fed rats, p<0.001). Furthermore, fructose-fed groups had higher fasting insulin resistance index (FIRI: p<0.001) than control rats. In contrast, vanadyl sulfate significantly decreased FIRI in the fructose fed- vanadyl treated groups (p<0.001) compared with fructose- fed animals. These results indicate that administration of low doses of vanadyl sulfate may be advantageous for preservation of the functional characteristics of pancreatic beta cells, probably by improving insulin action and thereby insulin resistance prevention
Insulin resistance, Vanadyl sulfate, Diabetes mellitus, Fasting insulin resistance index (FIRI), Triglyceride
http://ibj.pasteur.ac.ir/article-1-526-en.html
http://ibj.pasteur.ac.ir/article-1-526-en.pdf
Pasteur Institute of Iran
Iranian Biomedical Journal
1028-852X
2008-823X
7
4
2003
10
1
Sympathetic Gangliogenesis and Temporo-Spatial Glycoconjugates’s Terminal Sugars Distribution
183
186
EN
Mokhtar
Jafarpur
jafarpurmokhtar@yahoo.com
Y
Hasan
Mofidpur
N
Alireza
Fazel
N
Lectin binding histochemistry was performed on the developing sympathetic ganglionic cells to investigate the distribution and density of defined carbohydrate terminals on the cell surface glycoproteins during autonomic system morphogenesis. Sprague-Dauley rat embryos from 9th gestational day to birth were fixed and paraffinized. Serial sections of these specimens were incubated with different lectins, which were conjugated to horse raddish peroxidase (HRP). Diaminobenzidine was used to localize the HRP on the binding sites of lectins to terminal sugars. Among these lectins, soybean agglutinin reacted with migratory neural crest cells in early stages, from 9th to 13th day of gestation. These cells were moving ventrally in relation to the neural tube. In the late stages from days 14th to 21st, orange peel fungus agglutinin reacted with sympathetic migratory cells. Our results suggest that at each stage of sympathetic ganglia development, a specific glycoconjugate seems to be the key factor for development of the cells. We suggest that only one key specific terminal sugar is active in each stage of development, which might genetically regulated for particular stage of sympathetic gangliogenesis
Neural crest, Sympathetic ganglia, Lectin histochemistry
http://ibj.pasteur.ac.ir/article-1-527-en.html
http://ibj.pasteur.ac.ir/article-1-527-en.pdf
Pasteur Institute of Iran
Iranian Biomedical Journal
1028-852X
2008-823X
7
4
2003
10
1
Comparison of Mycelial Production by Candida Albicans Isolated from Different Sources
187
189
EN
Ali
Zarei Mahmoudabadi
zarei40@hotmail.com
Y
David B.
Drucker
N
The aim of this study was to compare the ability of 46 isolates of Candida albicans to produce mycelial form with oral source in three groups of individuals including, removable appliance wearers, non-wearers and oral medicine patients. Saliva samples were obtained from all subjects along with a foam imprint from the fitting surface of the upper removable appliance in the case of patients. Colonies that were green-blue were confirmed as C. albicans by the germ-tube test. The production of mycelia was measured in vitro using defined medium (Lee’s medium) during 24 h. The results indicate that the production of mycelia in C. albicans isolated from removable appliance wearers and oral medicine patients are significantly different (p<0.025).
Candida albicans, Mycelial form, Lee’s medium
http://ibj.pasteur.ac.ir/article-1-528-en.html
http://ibj.pasteur.ac.ir/article-1-528-en.pdf