ORIGINAL_ARTICLE
کاهش پروتئین کنسین، رشد سلول های سرطانی کبد را متوقف نموده، در آنها آپوپتوز ایجاد کرده و حساسیت شیمیایی در این سلول ها را افزایش می دهد
http://ibj.pasteur.ac.ir/article-1-1271-fa.pdf
2014-12-02
1
16
10.6091/ibj.1386.2014
Downregulation of Kinesin Spindle Protein Inhibits Proliferation, Induces Apoptosis and Increases Chemosensitivity in Hepatocellular Carcinoma Cells
Background: Kinesin spindle protein (KSP) plays a critical role in mitosis. Inhibition of KSP function leads to cell cycle arrest at mitosis and ultimately to cell death. The aim of this study was to suppress KSP expression by specific small-interfering RNA (siRNA) in Hep3B cells and evaluate its anti-tumor activity. Methods: Three siRNA targeting KSP (KSP-siRNA #1-3) and one mismatched-siRNA (Cont-siRNA) were transfected into cells. Subsequently, KSP mRNA and protein levels, cell proliferation, and apoptosis were examined in both Hep3B cells and THLE-3 cells. In addition, the chemosensitivity of KSP-siRNA-treated Hep3B cells with doxorubicin was also investigated using cell proliferation and clonogenic survival assays. Results: The expression of endogenous KSP at both mRNA and protein levels in Hep3B cells was higher than in THLE-3 cells. In Hep3B cells, KSP-siRNA #2 showed a further downregulation of KSP as compared to KSP-siRNA #1 or KSP-siRNA #3. It also exhibited greater suppression of cell proliferation and induction of apoptosis than KSP-siRNA #1 or KSP-siRNA #3 this could be explained by the significant downregulation of cyclin D1, Bcl-2, and survivin. In contrast, KSP-siRNAs had no or lower effects on KSP expression, cell proliferation and apoptosis in THLE-3 cells. We also noticed that KSP-siRNA transfection could increase chemosensitivity to doxorubicin in Hep3B cells, even at low doses compared to control. Conclusion: Reducing the expression level of KSP, combined with drug treatment, yields promising results for eradicating hepatocellular carcinoma (HCC) cells in vitro. This study opens a new direction for liver cancer treatment.
http://ibj.pasteur.ac.ir/article-1-1271-en.pdf
2014-12-02
1
16
10.6091/ibj.1386.2014
Apoptosis
Chemosensitivity
Doxorubicin
Hepatocellular carcinoma (HCC) cells
Kinesin spindle protein (KSP)
Chinh Chung
Doan
hungbiotech_uns@yahoo.com
1
AUTHOR
Ngoc Trung
Doan
2
AUTHOR
Quang Huy
Nguyen
3
AUTHOR
Minh Hoa
Nguyen
4
AUTHOR
Minh Si
Do
5
AUTHOR
Van Dong
Le
6
AUTHOR
ORIGINAL_ARTICLE
بررسی بیان توأم و انفرادی دو بیومارکرmRNA موجود در خون محیطی بیماران مبتلا به سرطان سلول های غیر کوچک ریه (NSCLC) با استفاده از تکنیک Real Time RT-PCR
http://ibj.pasteur.ac.ir/article-1-1252-fa.pdf
2014-11-08
17
22
10.6091/ibj.1397.2014
Expression of Two Basic mRNA Biomarkers in Peripheral Blood of Patients with Non-Small Cell Lung Cancer Detected by Real-Time RT-PCR, Individually and Simultaneously
Introduction: Although extensive research has been conducted on lung cancer markers, a singular clinically applicable marker has not been found yet. The objective of this study was to evaluate the sensitivity and the specificity of carcinoembryonic antigen (CEA) mRNA and lung-specific X protein (LUNX) mRNA biomarkers in peripheral blood to detect lung cancer individually and simultaneously. Methods: Thirty patients affected by lung cancer and 30 healthy individuals were studied in this research. Three vials of cDNA were made from each sample after taking peripheral blood samples and extracting total RNA. Each sample was examined by the real-time RT-PCR technique. The result from each vial was then compared with the sensitivity of overall marker. Results: The CEA mRNA was positive in 24 out of 30 lung cancer patients. Hence, its sensitivity was determined at 80%, differing significantly from that observed in healthy individuals, where 11 positive cases were seen. The overall sensitivity of this marker was significantly associated with positivity in vials 2 and 3 but not in vial 1. The LUNX mRNA was positive in 21 out of 30 patients, indicating 70% sensitivity. This finding significantly differed from that in healthy individuals. The overall sensitivity of this marker was significantly associated with positivity in vials 1 and 3, but not in vial 2. In 93.3% of the patients, at least one positive marker was observed. Conclusions: The mentioned mRNA could be suggested as sensitive and specific markers in peripheral blood for primary diagnosis of lung cancer.
http://ibj.pasteur.ac.ir/article-1-1252-en.pdf
2014-11-08
17
22
10.6091/ibj.1397.2014
Lung neoplasms
RNA
Carcinoembryonic antigen
Sensitivity and specificity
Shirin
Karimi
1
AUTHOR
Abdolreza
Mohamadnia
2
AUTHOR
Seyed Alireza
Nadji
3
AUTHOR
Reza
Yadegarazari
4
AUTHOR
Adnan
Khosravi
5
AUTHOR
Naghmeh
Bahrami
6
AUTHOR
Massoud
Saidijam
sjam110@yahoo.com
7
AUTHOR
ORIGINAL_ARTICLE
مقایسه میزان رونویسی ژن های میتوکندریایی TFAM, NRF1 و MT-CO1 در مراحل مختلف بلوغ تخمک های انسان
http://ibj.pasteur.ac.ir/article-1-1295-fa.pdf
2015-01-05
23
28
10.6091/ibj.1400.2015
Comparison of Mitochondrial-Related Transcriptional Levels of mitochondrial transcription factor A, Nuclear respiratory factor 1 and cytochrome c oxidase subunit 1 Genes in Single Human Oocytes at Various Stages of the Oocyte Maturation
Background: The aim of the current study was to assess the mRNA levels of two mitochondria-related genes, including nuclear-encoded NRF1 (nuclear respiratory factor 1), mitochondrial transcription factor A (TFAM), and mitochondrial-encoded cytochrome c oxidase subunit 1 (MT-CO1) genes in various stages of the human oocyte maturation. Methods: Oocytes were obtained from nine infertile women with male factor undergoing in vitro fertilization (IVF)/intra-cytoplasmic sperm injection protocol. Mitochondrial-related mRNA levels were performed by single-cell TaqMan Real-time PCR. Results: the expression level of the target genes was low at the germinal vesicle stage (P>0.05). Although the mRNA level of NRF1 gene remained stable in metaphase I, the mRNA level of TFAM and MT-CO1 increased significantly (P<0.05).In metaphase II, the expression level of all genes increased compared to metaphase I (P<0.05). Conclusion: The overexpression levels of NRF1, TFAM, and MT-CO1 genes are related to the oocyte maturation. Therefore, the current study could be used clinically to improve the success rate of IVF.
http://ibj.pasteur.ac.ir/article-1-1295-en.pdf
2015-01-05
23
28
10.6091/ibj.1400.2015
Transcription
Human oocyte
Real-time PCR
Marefat
Ghaffari Novin
1
AUTHOR
Mehrdad
Noruzinia
2
AUTHOR
Azra
Allahveisi
allavaisie@gmail.com
3
AUTHOR
Aboutaleb
Saremi
4
AUTHOR
Fateme
Fadaei Fathabadi
5
AUTHOR
Reza
Mastery Farahani
6
AUTHOR
Ali
Dehghani Fard
7
AUTHOR
Arash
Pooladi
8
AUTHOR
Ramin
Mazaherinezhad Fard
9
AUTHOR
Elham
Yousefian
10
AUTHOR
ORIGINAL_ARTICLE
بازسازی نورونی در مغز ایسکمیک هیپوکسیک بدنبال تجویز سلول های خون بند ناف
http://ibj.pasteur.ac.ir/article-1-1294-fa.pdf
2015-01-05
29
34
10.6091/ibj.1376.2015
Neuronal Cell Reconstruction with Umbilical Cord Blood Cells in the Brain Hypoxia-Ischemia
Background: Brain hypoxia-ischemia is a human neonatal injury that is considered a candidate for stem cell therapy. Methods: The possible therapeutic potential of human umbilical cord blood (HUCB) stem cells was evaluated in 14-day-old rats subjected to the right common carotid occlusion, a model of neonatal brain hypoxia-ischemia. Seven days after hypoxia-ischemia, rats received either saline solution or 4 × 105 HUCB cells i.v. Rats in control group did not receive any injection. After two weeks, rats were assessed using two motor tests. Subsequently, rats were scarified for histological and immunohistochemical analyses. Results: Our immunohistochemical findings demonstrated selective migration of the injected HUCB cells to the ischemic area as well as reduction in infarct volume. Seven days after surgery, we found significant recovery in the behavioral performance in the test group (12.7 +/- 0.3) compared to the sham group (10.0 +/-0.05), a trend which continued to day 14 (15.3 ± 0.3 vs. 11.9 ± 0.5, P<0.05). Postural and motor asymmetries at days 7 and 14 in the test group showed a significant decrease in the percentage of right turns in comparison to the sham group (75% and 59% vs. 97% and 96%, P<0.05). Conclusion: The results show the potential of HUCB stem cells in reduction of neurologic deficits associated with neonatal hypoxia-ischemia.
http://ibj.pasteur.ac.ir/article-1-1294-en.pdf
2015-01-05
29
34
10.6091/ibj.1376.2015
Hypoxia-Ischemia
Nerve cell
Umbilical Cord Blood
Hossein Ali
Ghaffaripour
1
AUTHOR
Mehdi
Jalali
JalaliM@mums.ac.ir
2
AUTHOR
Mohammad Reza
Nikravesh
3
AUTHOR
Masoumeh
Seghatoleslam
4
AUTHOR
Javad
Sanchooli
5
AUTHOR
ORIGINAL_ARTICLE
آنالیز مقایسه ای سلول های CD4+ T و CD8+ در بافت تومور، غده لنفاوی و خون محیطی افراد مبتلا به سرطان پستان
http://ibj.pasteur.ac.ir/article-1-1246-fa.pdf
2014-10-29
35
44
10.6091/ibj.1289.2014
Comparative Analysis of CD4+ and CD8+ T Cells in Tumor Tissues, Lymph Nodes and the Peripheral Blood from Patients with Breast Cancer
Background: CD4+ and CD8+ T cells are the main types of lymphocytes in cell-mediated immunity and play a central role in the induction of efficient immune responses against tumors. The frequencies of T cell subtypes in the peripheral blood and tumor tissues, and draining lymph nodes (dLN) can be considered as useful markers for evaluation of the immune system in cancers. Methods: In this study, the frequencies of CD4+ and CD8+ T cells in blood, tumor tissues, and dLN samples of breast cancer patients were compared with each other and with similar tissues from normal individuals. Immunophenotyping was carried out by flow cytometry and the expression levels of CXCL10, granzyme B, and mammaglobin were evaluated by real-time PCR. Results: In the peripheral blood, there were no differences in the T cell subsets between the patients and the normal individuals. The frequency of CD8+ T cells was significantly higher in tumor tissue than normal breast tissues while granzyme B expression was similar. Based on mammaglobin expression levels, dLN have been classified into micro- and macro-metastatic dLN. We found significantly lower frequency of CD4+ in macro-metastatic dLN than micro-metastatic dLN. CD8+ frequency was similar in both dLN however, granzyme B expression was higher in micro-metastatic ones. There was not any significant difference in CXCL10 expression between the two types of dLN. Conclusions: Based on our results, although the tumor does not affect the systemic immunity, tumoral cells affect the local immune system in the tumoral tissues and the metastatic dLN.
http://ibj.pasteur.ac.ir/article-1-1246-en.pdf
2014-10-29
35
44
10.6091/ibj.1289.2014
Breast neoplasms
CD4+ T lymphocyte
CD8+ T lymphocytes
Granzymes
CXCL10
Farhad
Riazi Rad
1
AUTHOR
Soheila
Ajdary
2
AUTHOR
Ramesh
Omranipour
3
AUTHOR
Mohammad Hossein
Alimohammadian
4
AUTHOR
Zahir
Hassan
hasan_zm@modares.ac.ir
5
AUTHOR
ORIGINAL_ARTICLE
اثر ضد تشنجی دانه نخود در مدلهای تجربی صرع: معرفی یک مولکول شیمیایی جدید ضد تشنج
http://ibj.pasteur.ac.ir/article-1-1270-fa.pdf
2014-12-02
45
50
10.6091/ibj.1391.2014
Anticonvulsant Effect of Cicer arietinum Seed in Animal Models of Epilepsy: Introduction of an active Molecule with Novel Chemical Structure
Background: Cicer arietinum (Chickpea) is one of the most important harvests in the world with high nutritional value. Lack of essential oils in the seeds of Chickpea is an advantage in search for drug-like molecules with less toxicity. We evaluated anticonvulsant effect of C. arietinum in common animal models of epilepsy. Methods: Dichloromethane extract was obtained from C. arietinum seeds by percolation. Acute toxicity of the extract was assessed in mice. Protective effect of the extract was examined against tonic seizures induced by maximal electroshock (MES 50 mA, 50 Hz, 1 s) in mice, clonic seizures induced by pentylenetetrazole (PTZ 60 mg/kg, i.p.) in mice, and electrical kindling model of complex partial seizures in rats. The extract was fractionated by n-hexane to f1 and f2 fractions. The extract and fractions underwent phytochemical analysis by thin layer chromatography. The active anticonvulsant fraction, f1, was subjected to matrix assisted laser desorption/ionization (MALDI) mass analysis. Results: The crude extract had neither toxicity up to 7 g/kg nor protective activity in MES and kindling models. However, it significantly inhibited clonic seizures induced by PTZ. f1 fraction mimicked protective effect of the extract. Phytochemical screening revealed the presence of considerable amount of alkaloids in the extract and fractions. Moreover, a novel structural class was detected in f1 fraction. Conclusion: Finding an anticonvulsant molecule pertaining to a new structural class in the seeds of C. arietinum promises an effective and inexpensive source of antiepileptic medication. Further studies are needed to identify its mechanism of action and more clues into its structure-activity relationship.
http://ibj.pasteur.ac.ir/article-1-1270-en.pdf
2014-12-02
45
50
10.6091/ibj.1391.2014
Anticonvulsants
Cicer
Kindling
Pentylenetetrazole (PTZ)
Soroush
Sardari
1
AUTHOR
Motahareh
Amiri
2
AUTHOR
Hourieh
Rahimi
3
AUTHOR
Mohammad
Kamalinejad
4
AUTHOR
Jamshid
Narenjkar
5
AUTHOR
Mohammad
Sayyah
sayyahm2@pasteur.ac.ir
6
AUTHOR
ORIGINAL_ARTICLE
بررسی ارتباط بین کدون 72 (Arg72Pro) ژن p53 و بیماری گلوکوم زاویه باز اولیه (POAG) در ایران
http://ibj.pasteur.ac.ir/article-1-1283-fa.pdf
2014-12-20
51
56
10.6091/ibj.1379.2014
Association between p53 Codon 72 (Arg72Pro) Polymorphism and Primary Open-Angle Glaucoma in Iranian Patients
Background: Glaucomatous neuropathy is a type of cell death due to apoptosis. The p53 gene is one of the regulatory genes of apoptosis. Recently, the association between the p53 gene encoding for proline at codon 72 and primary open-angle glaucoma (POAG) has been studied in some ethnic groups. This study is the first association analysis of POAG and p53 codon 72 polymorphism in Iranian patients. Methods: A cohort of 65 unrelated patients with POAG (age range from 12-62 years, mean ± SD of 40.16 ± 17.51 years) and 65 unrelated control subjects (without glaucoma, age range of 14-63 years, mean ± SD of 35.64 ± 13.61 years) were selected. In Iranian POAG patients and normal healthy controls, the p53 codon 72 polymorphism in exon 4 was amplified using polymerase chain reaction. The amplified DNA fragments were digested with the BstUI restriction enzyme, and the digestion patterns were used to identify the alleles for the polymorphic site. Results: Comparisons revealed significant differences in allele and genotype frequencies of Pro72Arg between POAG patients and control group. A higher risk of POAG was associated with allele Pro (OR = 2.1, 95% CI = 1.2–3.4) and genotype Pro/Pro (OR = 3.9, 95% CI = 0.13-12.7). Conclusion: The p53 Pro72 allele was more frequent in Iranian POAG patients than in the control group (P<0.05). The present findings show that the individuals with the Pro/Pro genotype may be more likely to develop POAG. However, additional studies are necessary to confirm this association.
http://ibj.pasteur.ac.ir/article-1-1283-en.pdf
2014-12-20
51
56
10.6091/ibj.1379.2014
Primary open-angle glaucoma (POAG)
Glaucoma
p53
Codon 72
Iran
Hossein
Neamatzade
1
AUTHOR
Reza
Soleimanizad
Dr.r.soleimanizad@gmail.com
2
AUTHOR
Aref
Atefi
3
Dept. of Microbial Biotechnology, Payam Noor University, Tehran, Iran
AUTHOR
Masoud
Zare-Shehneh
4
AUTHOR
Saba
Gharibi
5
AUTHOR
Abolfazl
Shekari
6
AUTHOR
Amir Bahman
Rahimzadeh
7
AUTHOR
8
AUTHOR
ORIGINAL_ARTICLE
بررسی همزمان سه ژنوتیپ C1236T, C3435T, G2677T/A در ژن MDR1 جمعیت شهر همدان ، غرب ایران
http://ibj.pasteur.ac.ir/article-1-1239-fa.pdf
2014-10-21
57
62
10.6091/ibj.1381.2014
Simultaneous Analysis of Multidrug Resistance 1(MDR1) C3435T, G2677T/A, and C1236T genotypes in Hamadan City Population, West of Iran
Background: One of the limitations in the treatment of common diseases such as cancer chemotherapy is development of multidrug resistance (MDR). Polymorphisms could alter the expression level of MDR1 gene, which plays an important role in MDR. In this research, the frequency of C3435T, C1236T, and G2677T/A polymorphisms of MDR1 gene was investigated in a large group of population from Hamadan city to provide a sample data resource. Methods: Peripheral blood (2 ml) was taken, and DNA extraction was carried out. Multiplexed mutagenically separated PCR, which was followed by polyacrylamide gel electrophoresis and silver staining, was applied to detect the mentioned polymorphisms in 935 individuals. Sequencing performed for confirmation of gel electrophoresis resulted in 10 random cases. In total, alleles and genotypes of 933 persons (776 women and 157 men) were determined. Results: The most frequent alleles of the polymorphisms were: 3435T, C1236, and G2677. The most frequent genotypes were: 3435C/T, 1236C/T, and 2677G/A, and their concurrent presence was also found as the most frequent simultaneous genotypes. There was not any meaningful difference among the prevalence of these genotypes in groups of men and women. Conclusion: Our results were close to those of other studies performed in Iran and compared to the other ethnic groups, which showed more similarity to Asian peoples than Europeans. As an aspect of personalized medicine, it could be used by chemotherapists to improve the routine methods of cancer treatment.
http://ibj.pasteur.ac.ir/article-1-1239-en.pdf
2014-10-21
57
62
10.6091/ibj.1381.2014
Gene polymorphism
Multi-drug resistance
Neoplasm
Massoud
Saidijam
1
AUTHOR
Hossein
Mahjub
2
AUTHOR
Nooshin
Shabab
3
AUTHOR
Reza
Yadegarazari
reza.yadegarazari@yahoo.com
4
AUTHOR
SHORT_COMMUNICATION
توزیع گلیکوکانژوگیت های سازمان دهنده های حرکتی در طی تکوین نخاع موش
http://ibj.pasteur.ac.ir/article-1-1293-fa.pdf
2015-01-05
63
68
10.6091/ibj.1298.2015
Glycoconjugates Distribution during Developing Mouse Spinal Cord Motor Organizers
Background: The aim of this research was to study the distribution and changes of glycoconjugates particularly their terminal sugars by using lectin histochemistry during mouse spinal cord development. Methods: Formalin-fixed sections of mouse embryo (10-16 fetal days) were processed for lectin histochemical method. In this study, two groups of horseradish peroxidase-labeled specific lectins were used: N-acetylgalactosamine, including Dolichos biflorus, Wisteria floribunda agglutinin (WFA), Vicia villosa, Glycine max as well as focuse-binding lectins, including tetragonolobus, Ulex europaeus, and Orange peel fungus (OFA). All sections were counterstained with alcian blue (pH 2.5). Results: Our results showed that only WFA and OFA reacted strongly with the floor plate cells from early to late embryonic period of developing spinal cord. The strongest reactions were related to the 14, 15, and 16 days of tissue sections incubated with OFA and WFA lectins. Conclusion: The present study demonstrated that cellular and molecular differentiation of the spinal cord organizers is a wholly regulated process, and &alpha-L-fucose, &alpha-D-GalNAc, and &alpha/&beta-D-GalNAc terminal sugars play a significant role during the prenatal spinal cord development.
http://ibj.pasteur.ac.ir/article-1-1293-en.pdf
2015-01-05
63
68
10.6091/ibj.1298.2015
Development
Glycoconjugates
Spinal cord
Elham
Vojoudi
1
AUTHOR
Vahid
Ebrahimi
2
AUTHOR
Alireza
Ebrahimzadeh-Bideskan
3
AUTHOR
Alireza
Fazel
fazela@mums.ac.ir
4
AUTHOR