REVIEW_ARTICLE آیا نیتریک اکساید یک هورمون است؟ http://ibj.pasteur.ac.ir/article-1-392-fa.pdf 2013-03-26 59 65 - Is Nitric Oxide a Hormone? Nitric oxide (NO) is a simple ubiquitous signaling molecule and plays important roles in almost every biological system. Recent evidences suggest that NO may act as an endocrine molecule. The aim of this review is considering available literature on endocrine roles of NO and/or its metabolites, i.e. nitrite and nitrate. Existing data suggest the idea that NO is a hormone that after production in tissues, it is stabilized and transported as nitrite and/or S-nitrosothiols in the blood to target cells. http://ibj.pasteur.ac.ir/article-1-392-en.pdf 2013-03-26 59 65 - Endocrine Hormone Nitric oxide (NO) Nitrate Nitrite Asghar Ghasemi 1 AUTHOR Saleh Zahediasl zahedi@endocrine.ac.ir 2 AUTHOR
ORIGINAL_ARTICLE بررس نیمه کمی بیان hoxA-11, lif and bteB1 mRNAs در بیماران مبتلا به اندومتریوز در مرحله ترشحی http://ibj.pasteur.ac.ir/article-1-393-fa.pdf 2013-03-26 66 72 - Semi-Quantitative Analysis of HOXA11, Leukemia Inhibitory Factor and Basic Transcriptional Element Binding Protein 1 mRNA Expression in the Mid-Secretory Endometrium of patients with endometriosis Background: HOXA11 and leukemia inhibitory factor (LIF) and basic transcriptional element binding protein1 (BTEB1) are expressed in endometrium throughout the menstrual cycle and show a dramatic increase during the mid-luteal phase at the time of implantation. In this case-control study, the expression pattern of these mRNA was evaluated in patients with endometriosis at the time of implantation. We also describe a semi-quantitative RT-PCR protocol optimized in our laboratory. Methods: Eight patients with endometriosis were considered as our case and 8 fertile women as control group. Expression levels of HOXA11, LIF and BTEB1 mRNA were measured in endometrium during the mid-secretory phase using semi-quantitative RT-PCR. Results: We describe the detailed procedure for the analysis of HOXA11, LIF and BTEB1 mRNA levels. Endometrial HOXA11 and LIF mRNA expression levels (normalized to ß-actin expression) were significantly decreased in endometrium of infertile patients with endometriosis compared with healthy fertile controls at the time of implantation (P<0.05). A similar trend was seen in BTEB1 mRNA expression. Conclusion: The results suggest that the alteration in expression pattern of the some genes could account for some aspects of infertility in endometriosis. http://ibj.pasteur.ac.ir/article-1-393-en.pdf 2013-03-26 66 72 - Endometriosis Implantation HOXA11 Leukemia inhibitory factor (LIF) Basic transcriptional element binding protein1 (BTEB1) Zohreh Alizadeh alizadeh@umsha.ac.ir 1 AUTHOR Naser Shokrzadeh 2 AUTHOR Massoud Saidijam 3 AUTHOR Marzieh Farimani Sanoee 4 AUTHOR
ORIGINAL_ARTICLE ارتباط پلیمرفیسم های Arg280His ،Arg194Trp و Arg399Gln در ژن XRCC1 و خطر کارسینوم تمایز یافته تیروئید در ایران http://ibj.pasteur.ac.ir/article-1-394-fa.pdf 2013-03-26 73 78 - Association of Arg194Trp, Arg280His and Arg399Gln Polymorphisms in X-Ray Repair Cross-Complementing Group 1 Gene and Risk of Differentiated Thyroid Carcinoma in Iran Background: X-ray repair cross-complementing group 1 (XRCC1) gene is a DNA repair gene and its non-synonymous single nucleotide polymorphisms (SNP) may influence DNA repair capacity which has been considered as a modifying risk factor for cancer development. Methods: A case-control study was conducted to investigate impact of three frequently studied polymorphisms (Arg194Trp, Arg280His and Arg399Gln) on developing differentiated thyroid carcinoma (DTC). Results: Increased risks for DTC were shown in homozygous (odds ratio [OR]: 3.66, 95% confidence interval [CI]: 0.38-35.60) and in dominant trait (OR: 1.22, 95% CI: 1.64-2.32) of Arg194Trp genotype. Also, for Arg280His genotype, an increased risk for DTC was shown in dominant trait (OR: 1.42, 95% confidence interval [CI]: 0.76-2.68), while a mildly reduction of risk for DTC (OR: 0.77, 95% [CI]: 0.50-1.17) was estimated in dominant Gln genotype of Arg399Gln. Considering combinatory effects of Arg194Trp and Arg280His genotypes on DTC, the calculated OR and 95% CI for being heterozygous for one of Arg194Trp or Arg280His genotypes were 1.57 and 0.90-2.74, respectively. Conclusion: Genotyping of codons 194, 280 and 399 in XRCC1 gene may use in risk assessment of DTC. http://ibj.pasteur.ac.ir/article-1-394-en.pdf 2013-03-26 73 78 - Endocrine related cancer DNA repair gene X-ray repair cross-complementing group 1 (XRCC1) gene Differentiated thyroid carcinoma (DTC) Pezhman Fard-Esfahani fard-esfahani@pasteur.ac.ir 1 AUTHOR Armaghan Fard-Esfahani 2 AUTHOR Shima Fayaz 3 AUTHOR Bahareh Ghanbarzadeh 4 AUTHOR Parinaz Saidi 5 AUTHOR Reyhaneh Mohabati 6 AUTHOR Seyed Kazem Bidoki 7 AUTHOR Mina Majdi 8 AUTHOR
ORIGINAL_ARTICLE تولید سلولهای دندریتیک مشتق از منوسیت در حضور هپارین و MCM و مقایسه فنوتیپ و عملکرد آنها http://ibj.pasteur.ac.ir/article-1-395-fa.pdf 2013-03-26 79 84 - Generation of Mature Monocyte-Derived Dendritic Cells in the Presence of Heparin and Monocyte Conditioned Medium: Phenotypic and Functional Comparison Background: Dendritic cells (DC) induce tumor or pathogen-specific T cell responses in humans. Several laboratories have developed culture systems, including maturation factors for human DC from peripheral blood monocytes. We comprehensively compared standard maturation stimulus, an autologous monocyte-conditioned medium (MCM), with heparin for their ability to promote uniformly mature DC that elicit T cell responses. Methods: A short (4-day) priming of plastic adherent monocytes with granulocyte-macrophage colony stimulating factor (GM-CSF) and IL-4 with or without heparin was followed by 48-hour incubation in MCM to generate fully mature and stable DC. Phenotypic and functional analyses were carried out using anti-CD14 and anti-CD83 monoclonal antibodies, and mixed lymphocyte reaction, respectively. Results: We found that fully matured DC with a large amount of cytoplasm and copious dendritic projections were visible at the end of culturing period in the presence of MCM, heparin and MCM plus heparin. Thus, DC generated with these maturation factors are non-adherent and have typical satellite morphology. Flow cytometric analysis using anti-CD14 (monocyte marker) and anti-CD83 (mature DC marker) revealed that expression of CD14 decreased in MCM plus heparin-treated DC, and the expression of CD83 was increased when heparin and MCM used as a maturation factor. Functionally, MCM and MCM plus heparin-treated DC showed stronger mixed leukocyte reaction than heparin alone. Conclusion: These results support the use of the MCM with heparin as maturation factor that could result in functionally mature monocyte-derived DC in comparison to either MCM or heparin alone. http://ibj.pasteur.ac.ir/article-1-395-en.pdf 2013-03-26 79 84 - Dendritic cell (DC) Maturation Monocyte Conditioned Medium (MCM) Heparin Nowruz Delirezh n.delirezh@urmia.ac.ir 1 AUTHOR Leila Majedi 2 AUTHOR Siamak Asri Rezaei 3 AUTHOR Hadi Ranjkeshzadeh 4 AUTHOR
ORIGINAL_ARTICLE بررسی اثر مصرف مزمن مورفین بر سطوح سرمی IL-6 و IL-10 و میزان رد آلوگرافت پوستی در موش صحرایی http://ibj.pasteur.ac.ir/article-1-396-fa.pdf 2013-03-26 85 91 - Chronic Morphine Consumption Increase Allograft Rejection Rate in Rat through Inflammatory Reactions Background: Although opioids suppressive effects on immune system function have been reported, this study demonstrates inflammatory reactions, such as production of pro-inflammatory cytokines and suppression of anti-inflammatory cytokines, are the main causes at organ’s allotransplantation rejection in chronic morphine-treated recipients. Methods: 28 rats were categorized in 4groups through intra-peritoneal administrations: control, sham, morphine treated animals (20 mg/kg injected of morphine daily until biopsy day), morphine and naloxane treated animals (20 mg/kg morphine and 2 mg/kg naloxane daily injected until biopsy day), which their donors were normal rats. The grafts were done at the 14th day of the experiment. Plasma interleukins levels (IL-6 and IL-10) in three sampling times were measured by ELISA. With almost 80% of macroscopic rejection signs in rats of one group, full thickness skin biopsy has been taken and histological parameters like perivascular infiltrates, epidermal changes, and stromal changes were detected. The statistical significance differences between the control and experimental groups were analyzed using the Kruskal-Wallis, followed by ANOVA post hoc test. Results: Accelerated skin allograft rejection by chronic morphine consumption can be resulted of increased IL-6 concentration and decreased IL-10. The enhancing effects of morphine on the graft inflammation were partially antagonized by Naloxane. It can illustrate the complexity of opiates and immune system connections and should be considered during organ transplantation of opiate addicts. Conclusion: Expansion of skin cells in recipient with chronic morphine administration history may be resulted in failure. http://ibj.pasteur.ac.ir/article-1-396-en.pdf 2013-03-26 85 91 - Morphine Skin allograft Inflammation IL-6 IL-10 Ali Rafati 1 AUTHOR Sommaye Hamzehie Taj hamzei@sums.ac.ir 2 AUTHOR Negar Azarpira 3 AUTHOR Assadollah Zarifkar 4 AUTHOR Ali Noorafshan 5 AUTHOR Parvaneh Najafizadeh 6 AUTHOR
ORIGINAL_ARTICLE اثر معکوس تزریق ال-آرژینین در آمیگدال مرکزی بر پاسخ فرارمکانی القائی نالوکسون در حیوانات شرطی شده با مرفین http://ibj.pasteur.ac.ir/article-1-397-fa.pdf 2013-03-26 92 99 - Reversal Effect of Intra-Central Amygdala Microinjection of L-Arginine on Place Aversion Induced by Naloxone in Morphine Conditioned Rats Background: Role of nitric oxide (NO) on expression of morphine conditioning using a solely classic task has been proposed previously. In this work, the involvement of NO on the expression of opioid-induced conditioning in the task paired with an injection of naloxone was investigated. Methods: Conditioning was established in adult male Wistar rats (weighing 200-250 g) using an unbiased procedure. Naloxone (0.05-0.4 mg/kg, i.p.), a selective antagonist of mu-opioid receptor, was administered once prior to morphine response testing. NO agents were administered directly into the central amygdala (CeA) prior to naloxone injection pre-testing. Results: Morphine (2.5-10 mg/kg, s.c.) produced a significant dose-dependent place preference in experimental animals. When naloxone (0.05-0.4 mg/kg, i.p.) was injected before testing of morphine (5 mg/kg, s.c.) response, the antagonist induced a significant aversion. This response was reversed due to injection of L-arginine (0.3-3 µg/rat), intra-CeA prior to naloxone administration. However, pre-injection of L-NAME (intra-CeA), an inhibitor of NO production, blocked this effect. Conclusion: The finding may reflect that NO in the nucleus participates in morphine plus naloxone interaction. http://ibj.pasteur.ac.ir/article-1-397-en.pdf 2013-03-26 92 99 - Morphine Naloxone L-arginine L-NAME Place aversion Sara Karimi 1 AUTHOR Manizheh Karami karami@shahed.ac.ir 2 AUTHOR Hedayat Sahraei 3 AUTHOR Mahnaz Rahimpour 4 AUTHOR
ORIGINAL_ARTICLE اثر عصاره پروآنتوسیانیدین هسته انگور بر استرس اکسیداتیو در رتهای با نفروپاتی دیابتی http://ibj.pasteur.ac.ir/article-1-398-fa.pdf 2013-03-26 100 106 - Effects of Grape Seed Proanthocyanidin Extract on Oxidative Stress Induced by Diabetes in Rat Kidney Background: This study examined the effect of grape seed proanthocyanidin extract (GSPE) on lipid peroxidation content and activity of tissue antioxidant enzymes, including catalase, superoxide dismutase and glutathione peroxidase in diabetic rats. Methods: Thirty male rats were divided into three groups of 10 rats each: control, diabetic and diabetic groups that received 500 mg/kg GSPE for 6 weeks. Diabetes was induced by a single intraperitoneal injection of streptozotocin (50 mg/kg body weight). Rats with fasting blood glucose levels above 250 mg/dl were used as diabetic animals. The first 24-hour urinary albumin excretion (UAE) was measured two weeks after diabetes induction and then each week until the end of the experimental period in all groups. Lipid peroxidation content and activities of catalase, superoxide dismutase and glutathione peroxidase were measured in kidney homogenate supernatants. Statistical significance of differences was assessed with one-way ANOVA by SPSS followed by Tukey's t-test. P<0.05 was assumed statistically significant. Results: UAE in diabetic nephropathy rats were significantly higher than in control. In addition, an increase in lipid peroxidation content and decrease in catalase, superoxide dismutase and glutathione peroxidase activities in kidney of diabetic nephropathy rats were observed. The GSPE administration did not affect on body weight, but significantly decreased lipid peroxidation and augmented the activities of antioxidant enzymes studied in kidney of diabetic nephropathy rats as well as reduced UAE and decreased kidney weight. Conclusion: The results suggested that GSPE could ameliorate diabetic nephropathy rats through reduction of oxidative stress and increase in renal antioxidant enzyme activity. http://ibj.pasteur.ac.ir/article-1-398-en.pdf 2013-03-26 100 106 - Grape seed proanthocyanidin extract (GSPE) Diabetic nephropathy Oxidative stress Antioxidant enzymes Esrafil Mansouri 1 AUTHOR Marzieh Panahi marzpanah@yahoo.com 2 AUTHOR Mohammad Ali Ghaffari 3 AUTHOR Ali Ghorbani 4 AUTHOR
ORIGINAL_ARTICLE بررسی سینتیک آنزیم میلوپراکسیداز در واکنش با یون مس http://ibj.pasteur.ac.ir/article-1-399-fa.pdf 2013-03-26 107 112 - Kinetic Investigation of Myeloperoxidase upon Interaction with Copper, Cadmium, and Lead Ions Background: Myeloperoxidase (MPO), which is abundantly expressed in neutrophils, catalyzes the formation of a number of reactive oxidant species. However, evidence has emerged that MPO-derived oxidants contribute to tissue damage and initiation and propagation of inflammatory diseases, particularly, cardiovascular diseases. Therefore, studying the regulatory mechanisms of the enzyme activity is of great importance. For clarifying some possible mechanism of the enzyme activity, kinetic investigations of MPO in the presence of Copper (Cu), Cadmium (Cd), and Lead (Pb) ions were carried out in vitro. Methods: MPO was partially purified from human white blood cells using ion-exchange and gel-filtration chromatography techniques. Its activity was measured spectrophotometrically by using tetramethyl benzidine (TMB) as substrate. Results: Purified enzyme had a specific activity of 21.7 U/mg protein with a purity index of about 0.71. Cu inhibited MPO activity progressively up to a concentration of 60 mM at which about 80% of inhibition achieved. The inhibition was non-competitive with respect to TMB. An inhibitory constant (Ki) of about 19 mM was calculated from the slope of repot. Cd and Pb did not show any significant inhibitory effect on the enzyme activity. Conclusion: The results of the present study may indicate that there are some places on the enzyme and enzyme-substrate complex for Cu ions. Binding of Cu ions to these places result in conformational changes of the enzyme and thus, enzyme inhibition. This inhibitory effect of Cu on the enzyme activity might be considered as a regulatory mechanism on MPO activity. http://ibj.pasteur.ac.ir/article-1-399-en.pdf 2013-03-26 107 112 - Myeloperoxidase Copper (Cu) Enzyme inhibition Cadmium (Cd) Lead (Pb) Maryam Shabani 1 AUTHOR Mohsen Ani 2 AUTHOR Ahmad Movahedian 3 AUTHOR Seyed Ziyae Aldin Samsam Shariat samsam@pharm.mui.ac.ir 4 AUTHOR