en
jalali
1386
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gregorian
2007
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مطالعه ایمونوسیتوشیمیایی تغییرات میکروتیوبول بر روی سلولهای لوکمی HL-60 تحت القا آپوپتوز
Immunocytochemical Study on Microtubule Reorganization in HL-60 Leukemia Cells Undergoing Apoptosis
Background: Microtubules (MT) are important components of cell cytoskeleton and play key roles in cell motility mitosis and meiosis. They are also the targets of several anticancer agents which indicating their importance in maintaining cell viability. Microtubular reorganization contributing to apoptotic morphology occurs in normal and neoplastic cells undergoing apoptosis induced by cytotoxic drugs. The aim of this study was to correlate the changes in the MT with behavior of the γ-tubulin in apoptotic cell, and to see if apoptitic MT showed biochemical characteristics of stable MT. Methods: Apoptosis was reorganization is an important factor of the mammalian cells response to apoptosis, and the altered properties of the MT did not reflect changes in function as apoptosis progresses. induced in the human leukemia cells (HL-60) by treatment with 1 µM of all-trans retinoic acid over a 5-day period. The time course of changes was assessed using flow cytometry, DNA fragmentation and immunocytochemistry in cells labeled for α-tubulins, acetylated α-tubulin and γ-tubulin. Results: The results indicated that γ-tubulin content is increased after cells have gone through the apoptosis with a diffuse cytoplasmic pattern. α-tubulin did not reveal any specific pattern of polymerization in apoptotic cells and acetylated α-tubulin content was also decreased in comparison with non-apoptotic cells. Conclusion: Our results support the idea that microtubule.
Apoptosis, Microtubule, γ-tubulin, Immunocytochemistry
209
214
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-103&slc_lang=en&sid=1
2010/04/21
1389/2/1
2015/09/14
1394/6/23
Shahin
Ahmadian
شهین
احمدیان
ahmadian@ibb.ut.ac.ir
00319475328460057095
00319475328460057095
Yes
Yaghub
Pazhang
یعقوب
پاژنگ
00319475328460057096
00319475328460057096
No
Ahmad
Shariftabrizi
احمد
شریف تبریزی
00319475328460057097
00319475328460057097
No
en
القاء توقف چرخه سلولی و آپوپتوز در سلول های تیروئیدی Thr-C1-PI33 توسط هیدروجنکوادافنین تخلیص شده از دندروستلرا لسرتی
G1 Phase Arrest and Apoptosis Induction in Human Thyroid Cancer Cell Line Thr.C1.PI33 by 3-Hydrogenkwadaphnin Isolated from Dendrostellera lessertii
Dendrostellera lessertii (Thymelaeaceae) is a toxic plant that grows in parts of Iran. The anti-proliferative properties of its crude methanol extract and one of its active components, 3-hydrogenkwadaphnin (3-HK), have been established using several cancer cell lines. Methods: In a further attempt to determine the mode of action, two groups of synchronously growing cells were treated with a single dose of 3-HK (3.5 nM) and/or a single dose of the crude extract (equivalent to 0.36 mg plant powder). Every 8 hours, the percentages of cells within G1, S, and G2-M phases were determined by flow cytometric (FCM) analysis electron microscopic pictures were taken after fixation with 2% glutaraldehyde. Results: Twelve hours after treatments, apoptotic cell death was confirmed by the observation of marked morphological changes of the plasma membrane as microvillar disappearance and the appearance of apoptotic bodies in the treated cells. FCM analyses revealed that the G1 phase arrest was under the influence of the pure substance. Conclusion: The results confirmed the previously drawn conclusion that the raw material and the pure substance from D. lessertii exert their anti-tumor effects through cell cycle arrest at G1 phase and diversion of cell fate toward programmed cell death.
Thyroid cancer, Apoptosis, Dendrostellera lessertii, Flow cytometry, Transmission electron microscopy
215
221
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-110&slc_lang=en&sid=1
2010/04/212010/04/26
1389/2/6
2015/09/142015/09/14
1394/6/23
Azin
Nowrouzi
آذین
نوروزی
00319475328460057098
00319475328460057098
No
Razieh
Yazdanparast
راضیه
یزدان پرست
yazdan@ibb.ut.ac.ir
00319475328460057099
00319475328460057099
Yes
en
فرونشاندن فعالیت تلومراز توسط پیریمتامین و اهمیت آن در سرطان
Suppression of Telomerase Activity by Pyrimethamine: Implication to Cancer
Although pyrimethamine (Tindurin™) appears to be effective in the prevention and treatment of some infectious diseases, very little information exists on its unpredictable properties. We design this study to evaluate its anti-tumoral effect on a model of cell line. Methods: The cytotoxic influence of Pyrimethamine on prostate cell line was investigated using an in vitro colometric assay. The potential modulatory effects on metastasis, apoptosis, and immortality characteristics of cells were assessed with gelatin zymography, terminal deoxyribonucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) assay and telomeric repeat amplification protocol, respectively. Results: Cytotoxicity analysis of pyrimethamine revealed a dose- dependent fashion. An apoptotic influence of pyrimethamine was also confirmed by data obtained from TUNEL assay. Dose-dependent inhibitory effect on matrix metalloproteinases (MMP) was seen in pyrimethamine. A potent inhibitory effect of pyrimethamine was also established by data achieved from TRAPeze telomerase detection kit. Conclusions: Collectively, as induction of apoptosis together with MMP and telomerase inhibition could be indicative of cancer treatment, pyrimethamine might be considered as a chemopreventative agent in cancer.
Telomerase, Cancer, Matrix metalloproteinases (MMP), Pyrimethamine
223
228
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-107&slc_lang=en&sid=1
2010/04/212010/04/262010/04/26
1389/2/6
2015/09/142015/09/142015/09/14
1394/6/23
Mohammad Reza
Khorramizadeh
محمدرضا
خرمی زاده
khoramza@sina.tums.ac.ir
00319475328460057100
00319475328460057100
Yes
Farshid
Saadat
فرشید
سعادت
00319475328460057101
00319475328460057101
No
Farhad
Vaezzadeh
فرهاد
واعظ زاده
00319475328460057102
00319475328460057102
No
Farnaz
Safavifar
فرناز
صفوی فر
00319475328460057103
00319475328460057103
No
Hassan
Bashiri
حسن
بشیری
00319475328460057104
00319475328460057104
No
Zahra
Jahanshiri
زهرا
جهانشیری
00319475328460057105
00319475328460057105
No
Majid
Momeny
مجید
مومنی
00319475328460057106
00319475328460057106
No
Abbas
Mirshafiey
عباس
میرشفیعی
00319475328460057107
00319475328460057107
No
en
بررسی اثر مهارکنندگی بوتیونین سولفوکسیمین در تکثیر ویروس سرخک و آپوپتوز ناشی از عفونت ویروسی با سویه AIK-HDC
Buthionine Sulfoximine Inhibits Cytopathic Effects and Apoptosis Induced by Infection with AIK-HDC Strain of Measles Virus
Measles virus (MV) is a highly contagious agent which causes a major health problem in developing countries. We studied the effect of buthionine sulfoximine (BSO) on the replication of an AIK-HDC strain of MV and its induced apoptosis in Vero cell lines. Methods: In this study, toxicity of BSO on Vero cells was investigated first, resulted in determination of sub-lethal or non-toxic concentration zone of BSO for cells. Next, anti-viral effect of BSO at various time limits was evaluated and virus titer was determined at each stage either as 50% tissue culture infective dose (TCID)50 or by plaque assay method. Using specific anti-measles IgG, anti-viral effect of BSO on MV replication cycle was evaluated through indirect immunofluorescence assay, meanwhile presence of viral RNA was investigated by RT-PCR and gel electrophoresis. Results: According to the experiments, BSO, at concentration of 50 μM, markedly inhibited the cytopathic effect (CPE) induced by MV. BSO also significantly inhibited apoptosis induced by MV. BSO either influences replication of MV genome, or may inhibit virion formation. Conclusion: These results suggest that the inhibition of CPE and apoptosis by BSO induced by MV may be associated with the effect of BSO on viral RNA genome. Therefore, it is suggested that MV infections can induce apoptosis through the activation of a common pathway that can be inhibited by BSO.
Measles virus (MV), AIK-HDC, Buthionine sulfoximine (BSO), Apoptosis
229
235
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-106&slc_lang=en&sid=1
2010/04/212010/04/262010/04/262010/04/26
1389/2/6
2015/09/142015/09/142015/09/142015/09/14
1394/6/23
Kaveh
Haratian
کاوه
هراتیان
retrokaveh@pasteur.ac.ir
00319475328460057108
00319475328460057108
Yes
Mahmood
Shamsi Shahrabadi
محمود
شمسی شهرآبادی
00319475328460057109
00319475328460057109
No
Soroush
Sardari
سروش
سرداری
00319475328460057110
00319475328460057110
No
en
بررسی اثر Epigallocatechin-3-Gallate در مدل تجربی آلزایمر در موش صحرایی: مطالعات رفتاری
The Beneficial Effect of (-)-Epigallocatechin-3-Gallate in an Experimental Model of Alzheimer’s disease in Rat: a Behavioral Analysis
Progressive cognitive decline is one of the hallmark symptoms of Alzheimer’s disease (AD) which can be modeled by β-amyloid injection into specific regions of brain. Since epigallocatechin-3-gallate (EGCG) is a potent antioxidant agent which its role against oxidative stress and inflammation has been shown in prior studies, we tried to determine whether EGCG administration protects against β-amyloid-induced memory and coordination impairment in rats. Methods: Animals (male Wistar rats) were divided into four groups: sham operated, EGCG-pretreated sham operated (sham + EGCG), untreated lesion (lesion), and EGCG-pretreated lesion (lesion + EGCG). Animals in lesion, lesion + EGCG, and sham + EGCG groups received sterile saline or saline plus EGCG (10 mg/kg) intraperitoneally one day pre-surgery and every other day for three weeks. The lesion was induced one day after EGCG pretreatment by injection of 4 µl of sterile saline or water containing 2 nmol/µl β-amyloid (1-40) into the hippocampal fissure. For behavioral analysis, psychomotor coordination (PMC) index and spontaneous alternation behavior were assessed using Rota-rod Treadmill and Y-maze, respectively at the third week post-lesion. Results: We found that β-amyloid (1-40) injection into hippocampus can decrease these behavioral indexes in lesion group in comparison with sham group which is similar to behavioral changes in AD. On the other hand, pretreatment with EGCG can improve the PMC index and spatial Y-maze alternation in the lesion + EGCG group in comparison with lesion group. Conclusion: We concluded that EGCG can be effective in restoring β-amyloid-induced behavioral derangements in rats regarding coordination and memory abilities.
Alzheimer’s disease (AD), Epigallocatechin gallate (EGCG), Hippocampus, Behavior
237
243
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-109&slc_lang=en&sid=1
2010/04/212010/04/262010/04/262010/04/262010/04/26
1389/2/6
2015/09/142015/09/142015/09/142015/09/142015/09/14
1394/6/23
Homa
Rasoolijazi
هما
رسولی جازی
homa_rassouli@yahoo.com
00319475328460057111
00319475328460057111
Yes
Mohammad Taghi
Joghataie
محمدتقی
جغتایی
00319475328460057112
00319475328460057112
No
Mehrdad
Roghani
مهرداد
روغنی
00319475328460057113
00319475328460057113
No
Maliheh
Nobakht
ملیحه
نوبخت
00319475328460057114
00319475328460057114
No
en
مقایسه تکوین جنین حاصل از روش روتین تزریق درون سیتو پلاسمی اسپرم با روش تزریق اسپرم با تکنیک پیزو بعد از تیمار اسپرم موش با کلسیم یونوفور
Comparison of Embryo Development between Intracytoplasmic and Piezo-Assisted Sperm Injection after Treating Mouse Sperms by Ca2+ Ionophore
The purpose of this study was to evaluate the efficiency of intracytoplasmic sperm injection (ICSI) and Piezo-assisted sperm injection after pretreatment with calcium ionophore (CaI) on the mouse embryo development. Methods: In this study, the conventional ICSI and Piezo-ICSI procedures were used. The efficacy of the methods was examined after mouse matured oocytes were fertilized with or without CaI-treated sperms. Results: Piezo-ICSI demonstrated significantly more favorable results, with a fertilization rate of 64% (conventional ICSI: 42%, P<0.001) and a cleavage rate of 73% (conventional ICSI: 58%, P<0.05). When the Piezo-ICSI procedure was performed with CaI-pretreated sperms, the cleavage rate significantly increased (92% vs. 73%, P<0.05). However, the fertilization rate did not change significantly (64% vs. 56%). Conclusion: The Piezo-ICSI accompanies with CaI-treated sperms is more efficient than the conventional ICSI method for fertilizing and thus obtaining more mouse embryos.
Intracytoplasmic sperm injection (ICSI), Piezo, Sperm activation, Embryo development
245
250
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-108&slc_lang=en&sid=1
2010/04/212010/04/262010/04/262010/04/262010/04/262010/04/26
1389/2/6
2015/09/142015/09/142015/09/142015/09/142015/09/142015/09/14
1394/6/23
Abdolhossein
Shahverdi
عبدالحسین
شاهوردی
00319475328460057115
00319475328460057115
No
Mansoureh
Movahedin
منصوره
موحدین
mansoure@modares.ac.ir
00319475328460057116
00319475328460057116
Yes
Mojtaba
Rezazadeh Valojerdi
مجتبی
رضازاده ولوجردی
00319475328460057117
00319475328460057117
No
Saeid
Kazemi Ashtiani
سعید
کاظمی آشتیانی
00319475328460057118
00319475328460057118
No
en
بیوشیمی مقاومت به حشره کش های پیرتروئید در سوسریهای آلمانی بیمارستانهای ساری، ایران
Biochemistry of Pyrethroid Resistance in German Cockroach (Dictyoptera, Blatellidae) from Hospitals of Sari, Iran
The German cockroach is an important household insect pest mechanically involved in transmission of a variety of diseases to humans. Different classes of insecticides have extensively been used for its control leading to insecticide resistance development. Hence, for an optimal control of this pest, the status and underlying mechanisms of insecticide resistance should be studied in this group of insects. Methods: Adult German cockroaches were collected from Imam and Bouali Cina Hospitals (Sari, Iran) and subjected to bioassay using jar test method. The results were compared to those of a susceptible laboratory strain. Biochemical assays of esterases, monooxigenases and glutathione S-transferase (GST) levels were undertaken on German cockroaches from Imam and Bouali Cina Hospitals and the results were compared deltamethrin 19.64 ± 2.9, 18.66 ± 3.45 and 8.64 ± 0.62 min for cypermethrin, respectively. The mean to a susceptible laboratory strain. Results: The LT50 values of the three strains were 20.24 ± 2.2, 19.87 ± 2.3 and 8.89 ± 0.26 for permethrin 19.3 ± 3.05, 17.6 ± 0.68 and 8.8 ± 0.99 for a-esterase activity of Imam and Bouali Cina Hospitals and susceptible strains were 6.941 × 10-4, 6.940 × 10-4 and 8.01 × 10-5 nmol/min/mg protein the mean b-esterase activity in those strains were 5.8 × 10-4, 4.25 × 10-4 and 7.28 × 10-5 nmol/min/mg protein the mean content of p450 in the above-mentioned strains were 5.64 × 10-6, 1.89 × 10-6 and 1.2 × 10-6 nmol/mg protein the mean GST activity were 6.66 × 10-2, 0.102 and 5.72 × 10-2 mmol/min/mg protein, respectively. Conclusion: The LT50 values and also the mean activity of all enzyme groups in field strains were significantly different from those of the susceptible strain, indicating a vigour tolerance to insecticides and pyrethroids in particular. Hence, insecticide resistance monitoring techniques should be put in place and also resistance management strategies and measures should be considered implementing in the area.
Pyrethroids, German cockroaches, Sari
251
258
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-105&slc_lang=en&sid=1
2010/04/212010/04/262010/04/262010/04/262010/04/262010/04/262010/04/26
1389/2/6
2015/09/142015/09/142015/09/142015/09/142015/09/142015/09/142015/09/14
1394/6/23
Ahmad Ali
Enayati
احمدعلی
عنایتی
tmaae@liverpool.ac.uk
00319475328460057119
00319475328460057119
Yes
Farzad
Motevalli Haghi
فرزاد
متولی حقی
00319475328460057120
00319475328460057120
No
en
سطح نیتریک اکساید در سمینال پلاسما و ارتباط آن با آسیبهای DNA اسپرم
Nitric Oxide Level in Seminal Plasma and Its Relation with Sperm DNA Damages
Evidence supports the involvement of nitric oxide (NO) in a Varity of male reproductive processes such as spermatogenesis, spermiogenesis, sperm motion, sperm metabolism and sperm capacitation. However, Low concentration of NO is essential in biology and physiology of spermatozoa, but high amounts of NO is toxic and has negative effects on sperm functions. On the other hand, it is established that high amounts of NO have detrimental effects on DNA. The integrity of sperm DNA is an important factor in successful fertility and embryo development. It is hypothesized that supra physiological concentrations of NO in seminal plasma cause sperm DNA damage. The aim of this study was to determine sperm DNA damage by comet assay and its correlation with NO level in seminal plasma of fertile and infertile men. Methods: Semen samples were collected from 45 patients and 70 healthy donors. The stable metabolites of NO (nitrite and nitrate) in seminal plasma were measured by Griess assay and DNA damage was determined using single cell gel electrophoresis (comet) assay method. Results: The NO concentration in the seminal plasma of infertile males was significantly higher than fertile males (5.74 ± 1.01 &muM/L vs. 3.88 ± 0.53 &muM/L). There was a significant positive correlation between the NO concentration and sperm DNA comet value in infertile males (P<0.01, R = 0.598). Conclusion: These results indicate that the overproduction of NO in genital tract of infertile males has a potential pathogenetic role in the reduction of sperm DNA integrity.
Nitric oxide (NO), Infertility, Sperm, DNA integrity
259
264
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-104&slc_lang=en&sid=1
2010/04/212010/04/262010/04/262010/04/262010/04/262010/04/262010/04/262010/04/26
1389/2/6
2015/09/142015/09/142015/09/142015/09/142015/09/142015/09/142015/09/142015/09/14
1394/6/23
Iraj
Amiri
ایرج
امیری
amiri44@yahoo.com
00319475328460057121
00319475328460057121
Yes
Nasrin
Sheikh
نسرین
شیخ
00319475328460057122
00319475328460057122
No
Rezvan
Najafi
رضوان
نجفی
00319475328460057123
00319475328460057123
No