en
jalali
1394
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gregorian
2016
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online
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Toxicity of Nanoparticles and an Overview of Current Experimental Models
Toxicity of Nanoparticles and an Overview of Current Experimental Models
Nanotechnology is a rapidly growing field having potential applications in many areas. Nanoparticles (NPs) have been studied for cell toxicity, immunotoxicity, and genotoxicity. Tetrazolium-based assays such as MTT, MTS, and WST-1 are used to determine cell viability. Cell inflammatory response induced by NPs is checked by measuring inflammatory biomarkers, such as IL-8, IL-6, and tumor necrosis factor, using ELISA. Lactate dehydrogenase (LDH) assay is used for cell membrane integrity. Different types of cell cultures, including cancer cell lines have been employed as in vitro toxicity models. It has been generally agreed that NPs interfere with either assay materials or with detection systems. So far, toxicity data generated by employing such models are conflicting and inconsistent. Therefore, on the basis of available experimental models, it may be difficult to judge and list some of the more valuable NPs as more toxic to biological systems and vice versa. Considering the potential applications of NPs in many fields and the growing apprehensions of FDA about the toxic potential of nanoproducts, it is the need of the hour to look for new internationally agreed free of bias toxicological models by focusing more on in vivo studies.
Cytotoxicity, in vitro, Metal nanoparticles, Toxicology, Review
1
11
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-573&slc_lang=en&sid=1
2015/08/19
1394/5/28
2015/08/19
1394/5/28
Haji
Bahadar
00319475328460055818
00319475328460055818
No
Faheem
Maqbool
00319475328460055819
00319475328460055819
No
Kamal
Niaz
00319475328460055820
00319475328460055820
No
Mohammad
Abdollahi
Mohammad.Abdollahi@UToronto.Ca
00319475328460055821
00319475328460055821
Yes
en
in vitro Anti-Proliferative Effect of Adiponectin on Human Endometriotic Stromal Cells through AdipoR1 and AdipoR2 Gene Receptor Expression
in vitro Anti-Proliferative Effect of Adiponectin on Human Endometriotic Stromal Cells through AdipoR1 and AdipoR2 Gene Receptor Expression
Background: Endometriosis is a complex disorder in reproductive age women which consist of stromal and epithelial cells implantation outside the uterine cavity. Adiponectin is a member of cytokine family with various metabolic roles and proliferation inhibition of many cancer cells. The aim of the present research was to determine adiponectin effect on human endometriotic stromal cells (ESCs) proliferation and their expression of adiponectin receptors. Methods: In this experimental study, endometrial biopsies (n = 7) were taken. ESCs isolation was done by enzymatic digestion and cell filtrations. ESCs of each biopsy were divided into four groups: 0 (control), 10, 100, and 200 ng/ml adiponectin concentrations in three different times (24, 48 or 72 h). The effect of adiponectin on ESC viability and expression of mRNA Adipo receptor1 (R1) and Adipo receptor2 (R2) was determined by Trypan blue staining and semi-quantitative RT-PCR, respectively. Data were analyzed by one-way ANOVA and unpaired student’s t-test, and P < 0.05 was considered statistically significant. Results: Adiponectin inhibited human endometriotic stromal cell proliferation in time- and dose-dependent manners significantly (P = 0.001). Expression of AdipoR1 and AdipoR2 gene receptors was increased in human ESCs significantly (P < 0.05). Conclusions: Adiponectin can suppress endometriosis by inhibiting ESC proliferation and increased AdipoR1 and AdipoR2 expression.
Adiponectin, Adiponectin receptors, endometriosis, stromal cells
12
17
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-579&slc_lang=en&sid=1
2015/08/192015/10/13
1394/7/21
2015/08/192015/10/13
1394/7/21
Somayeh
Bohlouli
00319475328460055822
00319475328460055822
No
Arezou
Rabzia
00319475328460055823
00319475328460055823
No
Ehsan
Sadeghi
00319475328460055824
00319475328460055824
No
Farzaneh
Chobsaz
00319475328460055825
00319475328460055825
No
Mozafar
Khazaei
Mkhazaei1345@yahoo.com
00319475328460055826
00319475328460055826
Yes
en
Quantitative Assessment of Proliferative Effects of Oral Vanadium on Pancreatic Islet Volumes and Beta Cell Numbers of Diabetic Rats
Quantitative Assessment of Proliferative Effects of Oral Vanadium on Pancreatic Islet Volumes and Beta Cell Numbers of Diabetic Rats
Background: Oral vanadyl sulfate (vanadium) induces normoglycemia, proliferates beta cells and prevents pancreatic islet atrophy in streptozotocin-induced diabetic rats. Soteriological method is used to quantitate the proliferative effects of vanadium on beta-cell numbers and islet volumes of normal and diabetic rats. Methods: Adult male Sprague-Dawley rats were made diabetic with intravenous streptozotocin injection (40 mg/kg). Normal and diabetic rats were divided into four groups. While control normal and diabetic (CD) groups used water, vanadium-treated normal (VTN) and diabetic (VTD) groups used solutions containing vanadyl sulfate (0.5-1 mg/mL, VOSO4 + 5H2O). Tail blood samples were used to measure blood glucose (BG) and plasma insulin. Two months after treatment, rats were sacrificed, pancreata prepared, and stereology method was used to quantitatively evaluate total beta cell numbers (TBCN) and total islet volumes (TISVOL). Results: Normoglycemia persisted in VTN with significantly decreased plasma insulin (0.19 +/- 0.08 vs. 0.97 +/- 0.27 ng/dL, P<0.002). The respective high BG (532 +/- 49 vs. 144 +/- 46 mg/dL, P<0.0001) and reduced plasma insulin (0.26 +/- 0.15 vs. 0.54 +/- 0.19 ng/dL, P<0.002) seen in CD were reversed in VTD during vanadium treatment or withdrawal. While the induction of diabetes, compared to their control, significantly decreased TISVOL (1.9 +/- 0.2 vs. 3.03 +/- 0.6 mm3, P<0.003) and TBCN (0.99 +/- 0.1 vs. 3.2 +/- 0.2 x 106, P<0.003), vanadium treatment significantly increased TISVOL (2.9 +/- 0.8 and 4.07 +/- 1.0 mm3, P<0.003) and TBCN (1.5 +/- 0.3 and 3.8 +/- 0.6 x 106, P<0.03). Conclusion: Two-month oral vanadium therapy in STZ-diabetic rats ameliorated hyperglycemia by partially restoring plasma insulin. This was through proliferative actions of vanadium in preventing islet atrophy by increasing beta-cell numbers.
Vanadium, Pancreas, Islet volumes, Rats
18
25
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-580&slc_lang=en&sid=1
2015/08/192015/10/132015/10/13
1394/7/21
2015/08/192015/10/132015/10/13
1394/7/21
Leila
Pirmoradi
00319475328460055827
00319475328460055827
No
Ali
Noorafshan
00319475328460055828
00319475328460055828
No
Akbar
Safaee
00319475328460055829
00319475328460055829
No
Gholam Abbas
Dehghani
dehghang@sums.ac.ir
00319475328460055830
00319475328460055830
Yes
en
Chronic Aerobic Exercise Decreases Lectin-Like Low Density Lipoprotein (LOX-1) Receptor Expression in Heart of Diabetic Rat
Chronic Aerobic Exercise Decreases Lectin-Like Low Density Lipoprotein (LOX-1) Receptor Expression in Heart of Diabetic Rat
Background: Overexpression of lectin-like low density lipoprotein (LOX-1) receptor plays an important role in hyperglycemia-induced vascular complications such as atherosclerosis. Based on the beneficial effects of exercise on preventing cardiovascular complications of diabetes, we aimed to examine the protective effects of aerobic exercise on expression of LOX-1 receptor and production of free radicals in the heart of diabetic rats. Methods: Four groups of rats were used: (n = 5 per group): sedentary normal, trained normal, sedentary diabetes and trained diabetes. Diabetes was induced by a single intraperitoneal injection of streptozotocin (50 mg/kg). The exercise protocol was consisted of swimming 30 min/day, 5 days/week for eight weeks. Plasma glucose was evaluated at initiation, weeks 4 and 8 of experiment. At the end of experiment, rats were sacrificed and the heart was removed for determination of nitrate, malondialdehyde, and LOX-1 gene expression. Results: In normal non-diabetic rats, the blood glucose level was <150 mg/dl however, the induction of diabetes resulted in levels more than >400 mg/dl. Gene expression of LOX-1 was increased in the heart of diabetic rats. Exercise reduced the gene expression of this protein in diabetic states without reducing the blood glucose. Finally, swimming exercise decreased the malondialdehyde and nitrate levels in heart tissue both in control and diabetic rats. Conclusion: Swimming exercise reduces heart expression of the LOX-1 receptor in accompany with reduction of free radicals production. Since these parameters are important in generation of diabetic complications, swimming exercise is a good candidate for reducing these complications.
Hyperglycemia, Diabetic complications, LOX-1 receptor, Exercise, Free radicals
26
32
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-576&slc_lang=en&sid=1
2015/08/192015/10/132015/10/132015/10/3
1394/7/11
2015/08/192015/10/132015/10/132015/10/3
1394/7/11
Simin
Riahi
00319475328460055831
00319475328460055831
No
Mohammad Taghi
Mohammadi
Mohammadi.mohammadt@yahoo.com
00319475328460055832
00319475328460055832
Yes
Vahid
Sobhani
00319475328460055833
00319475328460055833
No
Shima
Ababzadeh
00319475328460055834
00319475328460055834
No
en
Modulation of Lipopolysaccharide Stimulated Nuclear Factor kappa B Mediated iNOS/NO Production by Bromelain in Rat Primary Microglial Cells
Modulation of Lipopolysaccharide Stimulated Nuclear Factor kappa B Mediated iNOS/NO Production by Bromelain in Rat Primary Microglial Cells
Background: Microglial cells act as the sentinel of the central nervous system .They are involved in neuroprotection but are highly implicated in neurodegeneration of the aging brain. When over-activated, microglia release pro-inflammatory factors, such as nitric oxide (NO) and cytokines, which are critical in eliciting neuroinflammatory responses associated with neurodegenerative diseases. This study examined whether bromelain, the pineapple-derived extract, may exert an anti-inflammatory effect in primary microglia and may be neuroprotective by regulating microglial activation. Methods: Following the isolation of neonatal rat primary microglial cells, the activation profile of microglia was investigated by studying the effects of bromelain (5, 10, 20, and 30 µg/ml) on the levels of NO, inducible nitric oxide synthase (iNOS), and nuclear factor kappa B (NF-&kappaB) in microglia treated with lipopolysaccharide (LPS) (1 µg/ml). Data were analyzed using Student's t-test. P values less than 0.05 were considered to be statistically significant, compared with the LPS-treated group without bromelain. Results: Results showed that pretreatment of rat primary microglia with bromelain, decreased the production of NO induced by LPS (1 µg/ml) treatment in a dose-dependent manner. Bromelain (30 µg/ml) also significantly reduced the expression of iNOS at mRNA level and NF-&kappaB at protein level. Moreover, the study of mitochondrial activity in microglia indicated that bromelain had no cytotoxicity at any of the applied doses, suggesting that the anti-inflammatory effects of bromelain are not due to cell death. Conclusion: Bromelain can be of potential use as an agent for alleviation of symptoms in neurodegenerative diseases.
Microglia, Nitric oxide, NF-kappa B, Neuroimmunomodulation, Ananas
33
40
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-578&slc_lang=en&sid=1
2015/08/192015/10/132015/10/132015/10/32015/10/13
1394/7/21
2015/08/192015/10/132015/10/132015/10/32015/10/13
1394/7/21
Soraya
Abbasi Habashi
Abbasi.soraya86@yahoo.com
00319475328460055835
00319475328460055835
Yes
Farzaneh
Sabouni
00319475328460055836
00319475328460055836
No
Ali
Moghimi
00319475328460055837
00319475328460055837
No
Saeed
Ansari Majd
00319475328460055838
00319475328460055838
No
en
Prophylactic and Therapeutic Effects of Oleuropein on Reperfusion-Induced Arrhythmia in Anesthetized Rat
Prophylactic and Therapeutic Effects of Oleuropein on Reperfusion-Induced Arrhythmia in Anesthetized Rat
Background: This study was conducted to reveal that whether i.v. injection of oleuropein, the most potent polyphenolic antioxidant in olive leaf, has any effect on the magnitude of reperfusion arrhythmia in anesthetized rats or not. Methods: Eighty male Wistar rats were divided into 8 groups of 10 each: groups 1 and 5 were assigned as the prophylactic and treatment control groups, groups 2 and 6 as the prophylactic and treatment groups with lidocaine (10 mg/kg), groups 3 and 4 as the prophylactic groups with 10 and 50 mg/kg oleuropein (i.v.), and groups 7 and 8 as the treatment groups with 10 and 50 mg/kg oleuropein (i.v.), respectively. Reperfusion injury was induced by 5-min regional ischemia and 15-min reperfusion of left anterior descending coronary artery. Heart rate, blood pressure, and electrocardiogram were monitored throughout the procedure. Results: blood pressure was significantly decreased by infusion of 50 mg/kg oleuropein in groups 4 and 8, but unlike the lidocaine as a standard anti-arrhythmic drug in groups 2 and 5 had not significant effect on heart rate. The onset of arrhythmia in groups received oleuropein (groups 3, 4, 7, and 8) was significantly delayed. The mortality rate due to irreversible ventricular fibrillation was also significantly reduced in groups 3, 4, 7, and 8. The effect of lidocaine in groups 2 and 5 was more potent than that in oleuropein group. Conclusion: These findings indicate that i.v. injection of oleuropein possibly through its antioxidant activity reduces the magnitude of reperfusion-induced arrhythmia.
Oleuropein, Arrhythmia, Reperfusion, Rats
41
48
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-575&slc_lang=en&sid=1
2015/08/192015/10/132015/10/132015/10/32015/10/132015/09/28
1394/7/6
2015/08/192015/10/132015/10/132015/10/32015/10/132015/09/28
1394/7/6
Babak
Baharvand
00319475328460055839
00319475328460055839
No
Mansour
Esmailidehaj
ned1382@gmail.com
00319475328460055840
00319475328460055840
Yes
Jamileh
Alihosaini
00319475328460055841
00319475328460055841
No
Shirin
Bajoovand
00319475328460055842
00319475328460055842
No
Saeedeh
Esmailidehaj
00319475328460055843
00319475328460055843
No
Zeynab
Hafizi Barjin
00319475328460055844
00319475328460055844
No
en
Particular Distribution of Enterobacter cloacae Strains Isolated from Urinary Tract Infection within Clonal Complexes
Particular Distribution of Enterobacter cloacae Strains Isolated from Urinary Tract Infection within Clonal Complexes
Background: Based on biochemical properties, Enterobacter cloacae represents a large complex of at least 13 variant species, subspecies, and genotypes that progressively identified as the most species causing hospital-acquired infections. The aim of this study was to determine the relevance between phylogenetically related strains within the E. cloacae complex and the frequency of urinary tract infection caused by them. Methods: A 268-bp fragment was obtained from hsp60 gene for 50 clinical E. cloacae isolates from urine cultures of inpatients that admitted to six hospitals in Tehran, Iran during December 2012 to November 2013. The 107 nucleotide sequences were analyzed and the evolutionary distances of sequences were computed and neighbor-joining tree was calculated. Results: It showed that all of the genetic clusters have not an equal involvement in pathogenesis of urinary tract infections. Three superior clusters were found, together representing more than two third (80%) of the isolates (cluster VI with 25 members clusters III and VIII with 9 and 6 members, respectively) and some genetic clusters were absent (IV, X, XII, and xiii), some of which are supposed to be associated with plants and no human infection has been reported. Conclusions: This study, for the first time, reports the unequal contribution of E. cloacae complex subspecies and clusters in urinary tract infections in Iran and together with studies from other countries suggest that the subspecies of E.hormaechei subsp. Oharae is the most prevalent E. cloacae complex subspecies regardless of country under study.
Enterobacter cloacae complex, Urinary tract infection, hsp60
49
55
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-581&slc_lang=en&sid=1
2015/08/192015/10/132015/10/132015/10/32015/10/132015/09/282015/10/14
1394/7/22
2015/08/192015/10/132015/10/132015/10/32015/10/132015/09/282015/10/14
1394/7/22
Majid
Akbari
00319475328460055845
00319475328460055845
No
Bita
Bakhshi
b.bakhshi@modares.ac.ir
00319475328460055846
00319475328460055846
Yes
Shahin
Najar Peerayeh
00319475328460055847
00319475328460055847
No
fa
Quality Control of Widely Used Therapeutic Recombinant Proteins by a Novel Real-Time PCR Approac
Quality Control of Widely Used Therapeutic Recombinant Proteins by a Novel Real-Time PCR Approac
Background: Existence of bacterial host cell DNA contamination in biopharmaceutical products is a potential risk factor for patients receiving these drugs. Hence, the quantity of contamination must be controlled under the regulatory standards. Although different methods such as hybridization assays have been employed to determine DNA impurities, these methods are labor, intensive and rather expensive. In this study, a rapid real-time PCR test was served as a method of choice to quantify the E. coli host cell DNA contamination in widely used recombinant streptokinase (rSK), and alpha interferon (IFN-&alpha) preparations. Methods: A specific primer pair was designed to amplify a sequence inside the E. coli 16S rRNA gene. Serial dilutions of DNA extracted from E. coli host cells along with DNA extracted from Active Pharmaceutical Ingredients of rSK, and IFN-&alpha samples were subjected to an optimized real-time PCR assay based on SYBR Green chemistry. Results: The test enabled us to detect a small quantity of genomic DNA contamination as low as 0.0002 picograms, in recombinant protein-based drugs. For the first time, this study showed that DNA contamination in rSK and IFN-&alpha preparation manufactured in Pasteur Institute of Iran is much lower than the safety limit suggested by the US FDA. Conclusion: Real-time PCR is a reliable test for rapid detection of host cell DNA contamination, which is a major impurity of therapeutic recombinant protein to keep manufacturers’ minds on refining drugs, and provides consumers with safer biopharmaceuticals.
Host cell DNA contamination, Real-time PCR, Recombinant streptokinase (rSK), Alpha interferon (IFN-α)
56
62
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-560&slc_lang=en&sid=1
2015/08/192015/10/132015/10/132015/10/32015/10/132015/09/282015/10/142015/06/6
1394/3/16
2015/08/192015/10/132015/10/132015/10/32015/10/132015/09/282015/10/142015/06/6
1394/3/16
Babak
Mamnoon
00319475328460055848
00319475328460055848
No
Ahmad Reza
Kamyab
00319475328460055849
00319475328460055849
No
Dariush
Ilghari
00319475328460055850
00319475328460055850
No
Ali
Khamesipour
00319475328460055851
00319475328460055851
No
Mohsen
Karimi Arzenani
mkarimia@yahoo.com
00319475328460055852
00319475328460055852
Yes
Taghi
Naserpour Farivar
00319475328460055853
00319475328460055853
No
en
Role of Oxidative Stress in Modulating Unfolded Protein Response Activity in Chronic Myeloid Leukemia Cell Line
Role of Oxidative Stress in Modulating Unfolded Protein Response Activity in Chronic Myeloid Leukemia Cell Line
Background: Recently, it has been revealed that tyrosine kinase inhibitors (TKIs) act through inducing both oxidative and endoplasmic reticulum (ER) stress in chronic myeloid leukemia cells. However, ER stress signaling triggers both apoptotic and survival processes within cells. Nevertheless, mechanisms by which TKIs avoid the pro-survival effects are not clear. The aim of this study was to evaluate the potential role of oxidative stress in activity of unfolded protein response (UPR) survival pathway within K562 cell line. Methods: The expression of UPR survival target genes, Xbp1, and Grp94 (glucose requiring protein 94) was studied in single and combined exposure to oxidative and ER stress in K562 cell line by quantitative and qualitative PCR. Results: The expression of UPR-related survival gene Grp94 was hampered by exposing to oxidative stress in cell induced with ER stress. Conclusion: Interaction of oxidative and ER stress may role as a mediator influencing UPR signaling activity.
Unfolded protein response, Oxidative stress, Endoplasmic reticulum stress
63
67
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-577&slc_lang=en&sid=1
2015/08/192015/10/132015/10/132015/10/32015/10/132015/09/282015/10/142015/06/62015/10/3
1394/7/11
2015/08/192015/10/132015/10/132015/10/32015/10/132015/09/282015/10/142015/06/62015/10/3
1394/7/11
Ali
Bazi
m.baziali@gmail.com
00319475328460055854
00319475328460055854
Yes
Mohammad Reza
Keramati
00319475328460055855
00319475328460055855
No
Mehran
Gholamin
00319475328460055856
00319475328460055856
No