en
jalali
1392
10
1
gregorian
2014
1
1
18
1
online
1
fulltext
en
ارزیابی ایمنوژنسیته DNA واکسن بیان کننده ژن NS2 ویروس هپاتیت C در موش C57BL/6
Immunogenicity Evaluation of a DNA Vaccine Expressing the Hepatitis C Virus Non-Structural Protein 2 Gene in C57BL/6 Mice
Backgrounds: Most of the hepatitis C virus (HCV) infections elicit poor immune responses and 75% to 85% of cases become chronic therefore, the development of an effective vaccine against HCV is of paramount importance. In this study, we aimed to evaluate co-administration of HCV non-Structural Protein 2 and IL-12 DNA vaccines in C57BL/6 mice. Methods: A plasmid encoding full-length HCV NS2 protein (non-structural protein 2) was generated and used to vaccinate mice. Negative control (an empty expression vector) was also employed to evaluate the background response. To investigate immune responses against vaccine, C57BL/6 mice received three doses of the vaccine with a two-week interval. Cellular immunity was assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay for lymphocyte proliferation, lactate dehydrogenase release for cytotoxic T lymphocyte (CTL) activity and cytokine assay. Results: The findings demonstrated that immunization of mice with plasmid expressing HCV NS2 induced CTL response, interferon gamma production, and lymphocyte proliferation compared to negative control. The results also demonstrated that co-administration of IL-12 with the HCV NS2 plasmid induced significantly better immune response in C57BL/6 mice. Conclusion: DNA vaccine encoding HCV NS2 is an effective candidate that can trigger CTL-based immune response against HCV. In addition, the results suggested that combining the DNA vaccine approach with immune stimulatory cytokines may significantly enhance antigen-specific immune responses.
Hepatitis C virus (HCV), NS2 protein, DNA vaccine, IL-12
1
7
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-498&slc_lang=en&sid=1
2013/11/27
1392/9/6
2013/11/27
1392/9/6
Zahra
Gorzin
زهرا
گرزین
00319475328460056197
00319475328460056197
No
Ali Akbar
Gorzin
علی اکبر
گرزین
00319475328460056198
00319475328460056198
No
Alijan
Tabarraei
علیجان
تبرایی
00319475328460056199
00319475328460056199
No
Naser
Behnampour
ناصر
بهنامپور
00319475328460056200
00319475328460056200
No
Shiva
Irani
شیوا
ایرنی
00319475328460056201
00319475328460056201
No
Amir
Ghaemi
امیر
قائمی
ghaem_amir@yahoo.com
00319475328460056202
00319475328460056202
Yes
en
کاهش لانه گزینی و بروز ژنهای اینگرین بلاستوسیست موش در اثر تحریک تخمک گذاری با گنادوتروپین اگزوژن
Ovarian Stimulation by Exogenous Gonadotropin Decreases the Implantation Rate and Expression of Mouse Blastocysts Integrins
Background: Integrins are heterodimeric glycoprotein receptors that regulate the interaction of cells with extracellular matrix and may have a critical role in implantation. The aim of this study was to investigate the effect of ovulation induction on the expression of α4, αv, β1, and β3 integrins in mouse blastocyst at the time of implantation. Methods: The ovarian stimulated and non-stimulated pregnant mice were sacrificed on the morning of 5th day of pregnancy. The blastocysts were collected, and the expression of αv, α4, β1, and β3 integrins was examined using real-time RT-PCR and immunocytochemical techniques, then their ovarian hormones were analyzed at the same time. The implantation sites in uterine horns of other pregnant mice in both groups were determined under a stereomicroscope on the 7th day of pregnancy. Results: The results showed that the expression of αv, β1, and β3 integrins in both mRNA and protein levels was significantly lower in the ovarian stimulated group than the control group, and the maximum ratio of expression was belonged to β1 molecule (P>0.05). Conclusion: The implantation rate in superovulated mice was significantly lower than control mice. It was suggested that ovulation induction decreased the expression of αv, β1, and β3 integrins of mouse blastocysts.
Blastocyst, Integrins, Implantation
8
15
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-492&slc_lang=en&sid=1
2013/11/272013/08/26
1392/6/4
2013/11/272013/09/17
1392/6/26
Mehri
Fayazi
مهری
فیاضی
00319475328460056203
00319475328460056203
No
Mandana
Beigi Boroujeni
ماندانا
بیگی بروجنی
00319475328460056204
00319475328460056204
No
Mojdeh
Salehnia
مژده
صالح نیا
salehnim@modares.ac.ir
00319475328460056205
00319475328460056205
Yes
Behzad
Khansarinejad
بهزاد
خوانساری نژاد
00319475328460056206
00319475328460056206
No
en
نقش آنتی اکسیدانی اولئوروپین بر مغز میانی و نورونهای دوپا مینرژیک جسم سیاه در رتهای پیر
Antioxidant Role of Oleuropein on Midbrain and Dopaminergic Neurons of Substantia Nigra in Aged Rats
Background: Oleuropein is a phenolic compound which is present in the olive leaf extract. The purpose of the present study was to investigate the neuroprotective effect of oleuropein as an antioxidant agent on the substantia nigra in aged rats. Methods: Twenty 18-month-old Wistar rats (450-550 g) were randomly divided into control and experimental groups. The experimental group received a daily single dose of 50 mg/kg of oleuropein by oral gavage for 6 months. The control group received only distilled water. All rats were sacrificed two hours after the last gavage and the brains were removed and midbrains were cut. One part of the midbrains were homogenized and centrifuged. The tissue supernatant was assayed for lipid peroxidation (LPO) and antioxidant enzyme activities. The other part of midbrains fixed and embedded in paraffin, then processed for Nissl and immunohistochemistry (IHC) staining. Data was analyzed using SPSS by t-test. Differences were considered significant for P<0.05. Results: The level of LPO in midbrain of the rats was decreased significantly in the experimental group, but superoxide dismutase, catalase and glutathione peroxidase activities were increased in experimental group compared to control group (P<0.05). Morphometric analyses showed significantly that the experimental group had more neurons in substantia nigra pars compacta (SNc) either in Nissl or IHC staining when compared to control (P<0.05). Conclusion: The results of the present study indicate that treatment of the old rats with oleuropein reduces the oxidative damage in SNc by increasing the antioxidant enzyme activities.
Oleuropein, Aging, Dopaminergic neurons, Substantia nigra
16
22
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-496&slc_lang=en&sid=1
2013/11/272013/08/262013/11/20
1392/8/29
2013/11/272013/09/172013/11/20
1392/8/29
Maryam
Sarbishegi
مریم
سربیشگی
00319475328460056207
00319475328460056207
No
Fereshteh
Mehraein
فرشته
مهرآئین
femehra@yahoo.com
00319475328460056208
00319475328460056208
Yes
Mansooreh
Soleimani
منصوره
سلیمانی
00319475328460056209
00319475328460056209
No
en
پنتوکسی فیلین سطح سرمی مولکولهای چسبنده در بیماران مبتلا به آترواسکروز را کاهش می دهد
Pentoxifylline Decreases Serum Level of Adhesion Molecules in Atherosclerosis Patients
Background: Inflammation is involved in development, progression, and complications of atherosclerotic disease. Clinical studies have indicated that the level of monocyte chemoattractant protein 1 (MCP-1), IL-18, and adhesion molecules correlates with the severity of atherosclerosis and can predict future cardiovascular events. Experimental studies have shown pentoxifylline (PTX) reduces these factors in animal models. The purpose of the present pilot study was to evaluate effect of PTX on a group of inflammatory biomarkers in patients with coronary artery disease (CAD). Methods: Forty patients with angiographically documented CAD, who fulfilled inclusion and exclusion criteria, were entered in the double-blind, randomized, pilot clinical study. The patients were randomly given PTX (400 mg three times daily) or placebo (3 tab/day) for 2 months. Serum concentrations of MCP-1, IL-18, intercellular adhesion Molecule 1 (ICAM-1), and vascular cell adhesion molecule 1 (VCAM-1) were measured before and at the end of intervention by enzyme-linked immunosorbant assay. Results: Our study showed that the serum levels of ICAM-1 and VCAM-1 was decreased in the study population after two-month treatment (P<0.05). Conclusion: Based on the results of our pilot study, administration of PTX in CAD patients significantly decreases adhesion molecules levels.
Atherosclerosis, Inflammation, Pentoxifylline
23
27
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-491&slc_lang=en&sid=1
2013/11/272013/08/262013/11/202013/08/21
1392/5/30
2013/11/272013/09/172013/11/202013/10/19
1392/7/27
Amir Hooshang
Mohammadpour
امیر هوشنگ
محمدپور
00319475328460056210
00319475328460056210
No
Homa
Falsoleiman
هما
فالسلیمان
00319475328460056211
00319475328460056211
No
Jamal
Shamsara
جمال
شمس آرا
00319475328460056212
00319475328460056212
No
Ghazaleh
Allah Abadi
غزاله
الله آبادی
00319475328460056213
00319475328460056213
No
Ramin
Rasooli
رامین
رسولی
00319475328460056214
00319475328460056214
No
Mohammad
Ramezani
محمد
رمضانی
ramezanim@mums.ac.ir
00319475328460056215
00319475328460056215
Yes
en
مطالعه ملکولی و کلینیکی بیماران ایرانی مبتلا به فردریش آتاکسیا
Molecular and Clinical Investigation of Iranian Patients with Friedreich Ataxia
Background: Friedreich ataxia (FRDA) is an autosomal recessive disorder caused by guanine-adenine-adenine (GAA) triplet expansions in the FXN gene. Its product, frataxin, which severely reduces in FRDA patients, leads to oxidative damage in mitochondria. The purpose of this study was to evaluate the triple nucleotide repeated expansions in Iranian FRDA patients and to elucidate distinguishable FRDA clinical differences in these patients. Methods: A number of 22 Iranian patients (8 females and 14 males) from 16 unrelated families were studied. DNA was extracted from the peripheral blood of patients. The frequency and length of (GAA)n repeats in intron 1 of the FXN gene were analyzed using long-range PCR. In this study, the clinical criteria of FRDA in our patients and the variability in their clinical signs were also demonstrated. Results: An inverse relationship was observed between GAA repeat size and the age of onset. Although some distinguishable clinical features (such as limb ataxia and lower limb areflexia) were found in our patients, 90-95% of them had extensor plantar response and dysarthria. The results showed only one positive diabetes patient and also different effects on eye movement abnormality among our patients. Conclusion: The onset age of symptoms showed a significant inverse correlation with allele size in our patients (P>0.05). Based on comparisons of the clinical data of all patients, clinical presentation of FRDA in Iranian patients did not differ significantly from other FRDA patients previously reported.
Friedreich ataxia (FRDA), Frataxin, Mitochondria
28
33
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-495&slc_lang=en&sid=1
2013/11/272013/08/262013/11/202013/08/212013/10/13
1392/7/21
2013/11/272013/09/172013/11/202013/10/192013/10/19
1392/7/27
Mohammad Hossein
Salehi
محمد حسین
صالحی
00319475328460056216
00319475328460056216
No
Massoud
Houshmand
مسعود
هوشمند
massoudh@nigeb.ac.ir
00319475328460056217
00319475328460056217
Yes
Omid
Aryani
امید
آریانی
00319475328460056218
00319475328460056218
No
Behnam
Kamalidehghan
بهنام
کمالی دهقان
00319475328460056219
00319475328460056219
No
Elham
Khalili
الهام
خلیلی
00319475328460056220
00319475328460056220
No
en
تولید نانوذرات ژلاتین به عنوان یک سیستم تحویل برای بوواین سرم آلبومین
Producing Gelatin Nanoparticles as Delivery System for Bovine Serum Albumin
Background: Progress in the field of biology and biochemistry has led to the discovery of numerous bioactive peptides and proteins in the last few decades. Delivery of therapeutic proteins/peptides has received a considerable amount of attention in recent years. Methods: In this study, a two-step desolvation method was used to produce biodegradable hydrophilic gelatin nanoparticles (GNP) as a delivery system of protein model (BSA). The size and shape of the nanoparticles were examined by dynamic light scattering and scanning electron microscopy. Results: Particles with a mean diameter of 200-300 nm were produced and the percentage of entrapment efficiency was found to be 87.4. The optimum amount of theoretical BSA loading was obtained, the release of BSA was monitored in vitro, and the mechanism of release was studied. The BSA release profile showed a biphasic modulation characterized by an initial, relatively rapid release period, followed by a slower release phase. Conclusion: Results show that the two-step desolvation is an appropriate method for preparing GNP as a delivery vehicle for BSA.
Gelatin, Nanoparticles, Bovine serum albumin
34
40
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-497&slc_lang=en&sid=1
2013/11/272013/08/262013/11/202013/08/212013/10/132013/11/26
1392/9/5
2013/11/272013/09/172013/11/202013/10/192013/10/192013/11/26
1392/9/5
Bahareh
Azimi
بهاره
عظیمی
00319475328460056221
00319475328460056221
No
Parviz
Nourpanah
پرویز
نورپناه
pnx@aut.ac.ir
00319475328460056222
00319475328460056222
Yes
Mohammad
Rabiee
محمد
ربیعی
00319475328460056223
00319475328460056223
No
Shahram
Arbab
شهرام
عرب
00319475328460056224
00319475328460056224
No
en
پیشگیری از استئاتوهپاتیت غیر الکلی در رت توسط دو کمپلکس منگنز سالن
Prevention of Nonalcoholic Steatohepatitis in Rats by Two Manganese-Salen Complexes
Background: Nonalcoholic steatohepatitis (NASH), a progressive stage of nonalcoholic fatty liver disease (NAFLD), is characterized by steatosis with inflammation. Investigations have suggested that oxidative stress may play an important role in the progress of NAFLD to NASH. To provide further insights into beneficial effects of antioxidants in NASH prevention, we employed two manganese-superoxide dismutase/catalase mimetics, manganese N,N`-bis(salicyldene) ethylene diamine chloride (EUK-8) and manganese-3-methoxy N,N`-bis(salicyldene)ethylenediamine chloride (EUK-134), as two salen representatives and vitamin C as the standard antioxidant. Methods: Experimental NASH was induced in Male N-Mary rats by feeding a methionine/choline-deficient (MCD) diet to rats for 10 weeks. The rats (n = 5, 30 mg/kg/day) were randomly assigned to receive vitamin C, EUK-8, EUK-134 or vehicle orally. Results: Administration of salens together with the MCD diet reduced the serum aminotransferases, glutathione transferase and alkaline phosphatase, cholesterol, and LDL contents. In addition, the EUK-8 and EUK-134 improved NASH pathological features in liver of MCD-fed rats. Conclusion: EUK-8 and EUK-134 supplementation reduces NASH-induced abnormalities, pointing out that antioxidant strategy could be beneficial for prevention of NASH.
Fatty liver, Oxidative stress, Antioxidants
41
48
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-499&slc_lang=en&sid=1
2013/11/272013/08/262013/11/202013/08/212013/10/132013/11/262013/11/27
1392/9/6
2013/11/272013/09/172013/11/202013/10/192013/10/192013/11/262013/11/27
1392/9/6
Alireza
Rezazadeh
علیرضا
رضازاده
00319475328460056225
00319475328460056225
No
Razieh
Yazdanparast
راضیه
یزدان پرست
yazdan@ibb.ut.ac.ir
00319475328460056226
00319475328460056226
Yes
en
تاثیر همکشتی فیبروبلاست و Activin A بر رشد آزمایشگاهی فولیکول های پره آنترال موش
The Effects of Fibroblast Co-Culture and Activin A on in vitro Growth of Mouse Preantral Follicles
Background: This study was conducted to evaluate fibroblast co-culture and Activin A on in vitro maturation and fertilization of mouse preantral follicles. Methods: The ovaries from 12-14-day-old mice were dissected, and 120-150 μm preantral follicles were cultured individually in α-MEM as based medium for 12 days. A total number of 456 follicles were cultured in four conditions: (i) base medium as control group (n = 113), (ii) base medium supplemented with 30 ng/ml Activin A (n = 115), (iii) base medium co-cultured with mouse embryonic fibroblast (n = 113), and (iv) base medium supplemented with 30 ng/ml Activin A and co-cultured with fibroblast (n = 115). Rate of growth, survivability, antrum formation, ovulation, embryonic development and steroid production were evaluated. Analysis of Variance and Duncan test were applied for analyzing. Results: Both co-culture and co-culture + Activin A groups showed significant difference (P<0.05) in growth (on days 4, 6, and 8 of culture period) and survival rates. However, there was no significant difference in antrum formation, ovulation rate, and embryonic development of ovulated oocytes. There were significant differences (P<0.05) in the estradiol production on days 8, 10, and 12 between co-culture + Activin A and the control group. Progesterone production also was significant (P<0.05) in co-culture + Activin A group on days 6, 8, 10, and 12 compared to control group. Conclusion: Fibroblast co-culture and Activin A promoted growth and survivability of preantral follicles. However, simultaneous use of them was more efficient.
Fibroblast, Activin A, Follicle
49
54
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-512&slc_lang=en&sid=1
2013/11/272013/08/262013/11/202013/08/212013/10/132013/11/262013/11/272014/08/9
1393/5/18
2013/11/272013/09/172013/11/202013/10/192013/10/192013/11/262013/11/272014/08/9
1393/5/18
Abbasali
Karimpour Malekshah
1- عباسعلی
کریمپور ملکشاه
00319475328460056227
00319475328460056227
No
Mahmoud
Heidari
2- محمود
حیدری
mahmodh78@gmail.com
00319475328460056228
00319475328460056228
Yes
Kazem
Parivar
کاظم
پریور
00319475328460056229
00319475328460056229
No
Nasrin Sadat
Azami
نسرین سادات
اعظمی
00319475328460056230
00319475328460056230
No
en
مهار ژن sidB با استفاده از RNAi در Aspergillus nidulans
Down-Regulation of sidB Gene by Use of RNA Interference in Aspergillus nidulans
Background: Introduction of the RNA interference (RNAi) machinery has guided the researchers to discover the function of essential vital or virulence factor genes in the microorganisms such as fungi. In the filamentous fungus Aspergillus nidulans, the gene sidB plays an essential role in septation, conidiation and vegetative hyphal growth. In the present study, we benefited from the RNAi strategy for down-regulating a vital gene, sidB, in the fungus A. nidulans. Methods: The 21-nucleotide small interfering RNA (siRNA) was designed based on the cDNA sequence of the sidB gene in A. nidulans. Transfection was performed through taking up siRNA from medium by 6 hour-germinated spores. To evaluate the morphologic effects of siRNA on the fungus, germ tube elongation was followed. Moreover, total RNA was extracted and quantitative changes in expression of the sidB gene were analyzed by measuring the cognate sidB mRNA level by use of a quantitative real-time RT-PCR assay. Results: Compared to untreated-siRNA samples, a significant inhibition in germ tube elongation was observed in the presence of 25 nM of siRNA (42 VS 21 µM). In addition, at the concentration of 25 nM, a considerable decrease in sidB gene expression was revealed. Conclusion: Usage of RNAi as a kind of post-transcriptional gene silencing methods is a promising approach for designing new antifungal agents and discovering new drug delivery systems.
Aspergillus nidulans, sidB, RNAi
55
59
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-513&slc_lang=en&sid=1
2013/11/272013/08/262013/11/202013/08/212013/10/132013/11/262013/11/272014/08/92014/08/9
1393/5/18
2013/11/272013/09/172013/11/202013/10/192013/10/192013/11/262013/11/272014/08/92014/08/9
1393/5/18
Hamid
Eslami
حمید
اسلامی
00319475328460056231
00319475328460056231
No
Mohammad Reza
Khorramizadeh
محمدرضا
خرمی زاده
00319475328460056232
00319475328460056232
No
Mohammad Reza
Pourmand
محمدرضا
پورمند
00319475328460056233
00319475328460056233
No
Maryam
Moazeni
مریم
موذنی
00319475328460056234
00319475328460056234
No
Sassan
Rezaie
ساسان
رضایی
srezaie@tums.ac.ir
00319475328460056235
00319475328460056235
Yes