en
jalali
1376
12
1
gregorian
1998
3
1
2
2
online
1
fulltext
en
Transcriptional Coactivator CBP Facilitates Transcription Initiation and Reinitiation of HTLV-I and Cyclin D2 Promoter
HTLV-I is the etiologic agent for adult T-cell leukemia/lymphoma (ATL) and HTLV-I-associated myelopathy/tropical spastic paraparesis (HAM/TSP). Taxi, the major activator of this virus, is a 40- kDa (353 amino acid) phosphoprotein, predominantly localized in the nucleus of the host cell, which functions to trans-activate both viral and cellular promoters. Recently it has been shown that HTLV-I and /or Taxl expressing cells have altered gene expression of some of the cell cycle associated genes. Tax activates HTLV-I as well as number of other cellular promoters through CREB, NF-kB and SRE elements. In this study we analyzed the effect of a general coactivator, namely CBP, which may be involved in activation of many cellular genes. To analyze CBP transcription activation, we have utilized an in vitro transcription assay that allows the analysis of transcription initiation and reinitiation in the absence of chromatin effects. In this assay, which utilizes a G-free cassette downstream of the Tax-responsive 21 by repeats, polymerase II molecules responsible for the first round of transcription remain at the end of the G-free region, effectively blocking the complete elongation of reinitiated transcripts. Addition of Tax and a 682 amino acid fragment of CBP to the in vitro transcription reactions increased both full-length and shorter transcripts resulting from reinitiation. A CBP deletion mutant lacking the N-terminal activation domain was inactive. Preliminary data is also presented to show that, transactivation of HTLV-I and a cellular promoter, namely cyclin D2, takes place in early GO/G1 before the restriction point, "R", where Rb function has been implicated.
HTLV-I, Tax, cyclin, transcription
49
57
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-435&slc_lang=en&sid=1
2012/12/25
1391/10/5
1998/03/15
1376/12/24
Janet F.
Duvall
Janet F.
Duvall
00319475328460058026
00319475328460058026
No
Fatah
Kashanchi
فتاح
کاشانچی
00319475328460058027
00319475328460058027
Yes
en
Stress and Atherogenesis: Smooth Muscle Cell Mitogenic Activity and other Biochemical Changes Associated with Sera of "Stressed" Subjects
The proliferation of smooth muscle cells in the arterial wall (VSMC) is considered to play a key role in the development of atherosclerosis. To investigate the possible contribution of "stress" (experimentally-induced) to this process, blood from healthy volunteers, ages 21 to 65, screened to exclude major risk factors for coronary heart disease, was assayed for mitogenic activity after the subjects were exposed to one of 2 "stress" conditions. These consisted of a cognitive task with superimposed verbal harassment (group 1), and the cognitive task without harassment (group 2). Mitogenic activity was determined by studying the growth stimulatory effects of PDGF-depleted plasma derived serum (PDS) from "stressed" subjects added to cultured VSMC, as measured by incorporation of radioactive thymidine into DNA or increase in cell number. In addition, changes in the steady state of the mRNA for the c-myc protooncogene were also assayed in VSMC by Northern blot analysis, using sera showing the greatest differential "pre/post stress" mitogenic activity. Blood pressure (BP), heart rate (HR), cortisol, and serum total and HDL cholesterol were also evaluated. All measurements were made immediately before (baseline) and after a 30 min interval. Analysis of the data revealed that there were 33% of subjects in group 1 with an increase of thymidine incorporation 15% or greater than baseline, versus 21% in group 2. The average increases were 45% and 30%. A higher percentage (35-42%) of subjects in group 1 responded with increases in systolic and diastolic blood pressure, compared to subjects in group 2 (1520%) the average in blood pressure was 10-15%. Similarly, more subjects (52%) in group 1 had an elevated (average 10-15%) serum cortisol, compared to the 42% in group 2 subjects. HR, total HDL cholesterol showed slight changes only. These results suggest that psychoactive factors may affect cardiovascular systems via rapid elicited rises in serum mitogenic activity for VSMC.
Stress, atherogenesis, smooth muscle cell proliferation
59
70
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-436&slc_lang=en&sid=1
2012/12/252012/12/25
1391/10/5
1998/03/152013/03/27
1392/1/7
Joseph M.
Wu
Joseph M.
Wu
00319475328460058028
00319475328460058028
Yes
William H.
Gutstein
William H.
Gutstein
00319475328460058029
00319475328460058029
No
en
Administration of Large Volume Hypertonic Solutions for Resuscitation of Hemorrhagic Shock in Rabbit
An animal model was designed to study the effect of large volume Hypertonic Solutions (HTS) for resuscitation following a hypovolemic shock. Resuscitation in hypovolemic animals was performed by infusion of 15 ml/kg of normal saline (group I), 5% sodium bicarbonate (NaHCO3) (group II), 5% sodium chloride (NaCI) and 7.5 % NaHCO3 1:1 VN (group III), 5% NaC1 (group IV), and 5% NaCl and 5% serum albumin 2:1 VN (group V) via jugular vein. All animals were monitored sixty minutes post infusion. Then they were sacrificed to determine Brain Water Content (BWC), pathological investigation of heart, aorta and pulmonary tissues. Two other groups were also used for determination of normal BWC (group N) and aftershock BWC (group S). Mean Arterial Pressure (MAP) of groups H, IV and V were statistically different from group I (P <0.05). Albumin concentration and urine output were increased markedly in group V (P <0.05). Serum osmolality in groups III, IV, and V were significantly different from the first group (P <0.05). Acid-base parameters in groups II and III were raised toward normal, and they showed significant differences from group I (P <0.05). BWC were decreased in groups treated with HTS (p <0.05). No statistical differences were observed in pulse pressure, heart and respiration rates, sodium and potassium concentrations, and urine osmolality between the groups. No detectable pathological changes were observed in heart, aorta and pulmonary tissues.
Hemorrhagic shock, Hypertonic solution, Blood pressure, Acidosis, Urinary output
71
77
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-437&slc_lang=en&sid=1
2012/12/252012/12/252012/12/25
1391/10/5
1998/03/152013/03/271998/03/15
1376/12/24
Mehdi
Nematbakhsh
مهدی
نعمت بخش
00319475328460058030
00319475328460058030
Yes
Mehrnoosh
Moghaddassi
مهرنوش
مقدسی
00319475328460058031
00319475328460058031
No
Nepton
Soltani
نپتون
سلطانی
00319475328460058032
00319475328460058032
No
Seyyed Hossein
Samarian
سید حسین
سمریان
00319475328460058033
00319475328460058033
No
Parvin
Rajabi
پروین
رجبی
00319475328460058034
00319475328460058034
No
en
Cloning and Expression of Thermus Aquaticus DNA Polymerase Gene, Using a Thermo-Inducible Expression Vector
DNA polymerase gene from Thermus aquaticus strain YT1 was amplified using VENTTM DNA po-lymerase and cloned under the control of X.PR promoter and expression was induced by a shift in tern perature. The culture was then sonicated, and after centrifugation the lysate was treated with polyethyleneimine followed by a salting-out step. Finally the protein was precipitated with ammonium sulfate and fractionated by gel filtration. The resulting enzyme preparation was stable and active.
Taq DNA polymerase, PCR, Thermal induction
79
82
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-438&slc_lang=en&sid=1
2012/12/252012/12/252012/12/252012/12/25
1391/10/5
1998/03/152013/03/271998/03/151998/03/15
1376/12/24
Saeid
Bouzari
سعید
بوذری
00319475328460058035
00319475328460058035
Yes
Veladimir O.
Rechinsky
Veladimir O.
Rechinsky
00319475328460058036
00319475328460058036
No
en
Isolation of Legionella Pneumophila Serogroups 1, 8 & 10 (Causative Agents of Legionnaires' Disease) from Water Sources in Tehran, Iran
In this survey, 187 samples collected from 9 different sources of water in Tehran such as: water coolers, neonates incubators, water bathes (37° C), little pools in parks or squares, cooling towers, hot or cold water taps, were examined for Legionnaires' disease agents. Inoculating water samples on selective and nonselective media resulted in isolation of several strains of gram negative bacteria. Among them, seven strains of Legionella pneumophilla which did not grow on any of the above media, were identified by indirect fluorescent antibody technique (IFA) and biochemical tests. Following confirmation by "Center National De Reference Des Legionelloses, Faculte of Medicine Alexis Carrel, Lyon", the Center identified Legionella pneumophila of serogroups of 1, 8 and 10. Out of 6 hospitals, 5 showed contaminated water having pneumonic Legionellosis agents.
Legionnaires' disease, Legionella
83
87
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-439&slc_lang=en&sid=1
2012/12/252012/12/252012/12/252012/12/252012/12/25
1391/10/5
1998/03/152013/03/271998/03/151998/03/151998/03/15
1376/12/24
Mojtaba
Moosavian
مجتبی
موسویان
00319475328460058037
00319475328460058037
Yes
Bahram
Fathollahzadeh
بهرام
فتح الله زاده
00319475328460058038
00319475328460058038
No
Kazem
Amoli
کاظم
آملی
00319475328460058039
00319475328460058039
No
Nasrin
Moazzami
نسرین
معظمی
00319475328460058040
00319475328460058040
No
en
Detection Of Toxoplasma Gondii and Human Cytomegalovirus DNA in Blood from Transplant Recipients Using Multiplex Nested Polymerase Chain Reaction
Evidences from many studies suggested a polymerase chain reaction (PCR) as a valuable method for diagnosing infectious disease in the transplant recipients. We used this method for detection of Toxoplasma, gondii and human cytomegalovirus in blood specimens from patients after bone marrow or kidney transplantation. DNA of both infectious agents were detected using two separate sets of nested primers in the PCR. The conditions for a multiplex nested PCR providing simultaneous identification of both pathogens in one tube were optimized. This assay provides an application in clinical research for diagnosis of infections in post-transplant recipients.
Toxoplasma Gondii, HCMV, Multiplex PCR
89
93
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-440&slc_lang=en&sid=1
2012/12/252012/12/252012/12/252012/12/252012/12/252012/12/25
1391/10/5
1998/03/152013/03/271998/03/151998/03/151998/03/151998/03/15
1376/12/24
Nasser
Khodai Booran
ناصر
خدایی بوران
00319475328460058041
00319475328460058041
Yes
Mahdi
Assmar
مهدی
آسمار
00319475328460058042
00319475328460058042
No
A
Hovanesian
ا
هوانسیان
00319475328460058043
00319475328460058043
No
N.
Nikolaova
Valery V.
Bakayev
00319475328460058044
00319475328460058044
No
Valery V.
Bakayev
N.
Nikolaova
00319475328460058045
00319475328460058045
No