en
jalali
1388
4
1
gregorian
2009
7
1
13
3
online
1
fulltext
en
ترمیم ضایعه نخاعی بوسیله پیوند همزمان نورون های حرکتی مشتق از سلول های بنیادی جنینی و سلول های پوشاننده پیاز بویایی
Repair of Spinal Cord Injury by Co-Transplantation of embryonic Stem Cell-Derived Motor Neuron and Olfactory Ensheathing Cell
Background: The failure of regeneration after spinal cord injury (SCI) has been attributed to axonal demyelination and neuronal death. Cellular replacement and white matter regeneration are both necessary for SCI repair. In this study, we evaluated the co-transplantation of olfactory ensheathing cells (OEC) and embryonic stem (ES) cell-derived motor neurons (ESMN) on contused SCI. Methods: OEC cultured from olfactory nerve rootlets and olfactory bulbs. ESMN was generated by exposing mouse ES cells to retinoic acid and sonic hedgehog. Thirty female rats were used to prepare SCI models in five groups. Control and medium-injected groups was subjected to induce lesion without cell transplantation. OEC or ESMN or both were transplanted into the site of the lesion in other groups. Results: The purity of OEC culture was 95%. Motor neuron progenitor markers (Olig2, Nkx6.1 and Pax6) and motor neuron markers (Isl1, Isl2 and Hb9) were expressed. Histological analysis showed that significantly more (P<0.001) spinal tissue was spared in OEC, ESMN and OEC+ ESMN groups but the OEC+ ESMN group had a significantly greater percentage of spared tissue and myelination than other groups (P< 0.05). The numbers of ESMN in co-transplanted group were significantly higher than ESMN group (P<0.05). A significant (P<0.05) recovery of hindlimb function was observed in rats in the transplanted groups. Conclusion: We found that the co-transplantation of ESMN and OEC into an injured spinal cord has a synergistic effect, promoting neural regeneration, ESMN survival and partial functional recovery.
Spinal cord injury (SCI), Embryonic stem (ES) cell, Motor neuron, Olfactory ensheathing cell (OEC)
125
135
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-36&slc_lang=en&sid=1
2010/04/7
1389/1/18
2013/03/26
1392/1/6
Mohammad
Salehi
محمد
صالحی
00319475328460056878
00319475328460056878
No
Parichehr
Pasbakhsh
پریچهر
پاس بخش
00319475328460056879
00319475328460056879
No
Masoud
Soleimani
مسعود
سلیمانی
00319475328460056880
00319475328460056880
No
Mehdi
Abbasi
مهدی
عباسی
00319475328460056881
00319475328460056881
No
Gholamreza
Hasanzadeh
غلامرضا
حسن زاده
00319475328460056882
00319475328460056882
No
Mohammad Hossein
Modaresi
محمدحسین
مدرسی
00319475328460056883
00319475328460056883
No
Aligholi
Sobhani
علیقلی
سبحانی
sobhania@tums.ac.ir
00319475328460056884
00319475328460056884
Yes
en
تمایز سلول های استرومایی مغز استخوان به نورون های شبه گاباارژیک در محیط کشت
In vitro Transdifferentiation of Bone Marrow Stromal Cells into GABAergic-Like Neurons
Background: Cell therapy of many neurodegenerative diseases using bone marrow stromal cells (BMSC) requires the differentiation of BMSC into neuronal subtype. However, the transdifferentiation of BMSC into GABAergic phenotype requires more investigation. Methods: In this study, BMSC of adult female rats were pre-induced into neuroblast-like cells using 1 mM β-mercaptoethanol (βME) and 10 M retinoic acid (RA), followed by 40 mM potassium chloride as inducer. The BMSC were evaluated by fibronectin as well as Oct-4. The percentage of nestin, neurofilaments (NF 68, NF 160, and NF 200) and GABA immuno-reactive cells was used to evaluate the GABAergic differentiation at the pre-induction and induction stages. The statistical analysis was carried out using unpaired student's t-test and ANOVA with Tukey's multiple comparison. Results: The BMSC in the fourth passage expressed fibronectin up to 91.24 ± 0.82%. The pre-induced cells after 2 days of RA exposure showed the expression of neuroblastic markers of nestin and NF68 (81.56 ± 2.64% and 82.12 ± 2.65%, respectively). The yield of GABAergic neurons with β-ME for 1 h and RA as pre-inducer for 2 days followed by potassium chloride as inducer (40 mM for 3 days) was 60.64% ± 1.97%. In addition, NF160 and NF200 were detected in the transdifferentiated cells. RT-PCR showed no expression of Oct-4 after the induction and pre-induction stages. Conclusion: GABAergic-like neurons obtained from BMSC can be potentially used in cell transplanting for some neurodegenerative disorders.
Bone marrow stromal cells (BMSC), GABAergic-like neurons, Transdifferentiation, Cell therapy
137
143
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-37&slc_lang=en&sid=1
2010/04/72010/04/7
1389/1/18
2013/03/262013/03/26
1392/1/6
Payam
Mohammad-Gharibani
پیام
محمد غریبانی
00319475328460056844
00319475328460056844
No
Taki
Tiraihi
تقی
طریحی
ttiraihi@yahoo.com
00319475328460056845
00319475328460056845
Yes
Jalil
Arabkheradmand
جلیل
عرب خردمند
00319475328460056846
00319475328460056846
No
en
تولید آنتی ژن نوترکیب GRA8از انگل توکسوپلاسما گوندی در باکتری اشرشیاکلی
Bacterial Production of Dense Granule Antigen GRA8 of Toxoplasma gondii
Background: Dense granule antigens (GRA antigens) of Toxoplasma gondii induce strong antibody response in humans and are considered as useful diagnostic antigens. Previous studies reported expression of amino terminal GRA8 protein in fusion with large tags such as glutathione-S-transferase. The present study aimed to produce soluble full length immunogenic GRA8 in bacteria. Methods: GRA8 complementary DNA (cDNA), encoding amino acids 24 to 258, was amplified from tachyzoites of RH strain and cloned in prokaryotic expression vector pET-28b(+). Expression of recombinant GRA8 (rGRA8) was analyzed by SDS-PAGE. Antigenicity and immunogenicity of the protein were evaluated by Western-blotting. Results: The cloned gene fragment exhibited complete similarity with the published sequence of gra8 gene by sequence analysis. rGRA8 was expressed in Escherichia coli in fusion with a very small tag and the soluble protein was purified by immobilized metal ion affinity chromatography. In immunoblot, serum sample from a rabbit immunized with rGRA8 recognized a single antigen of T. gondii tachyzoite at the expected molecular weight of native GRA8. Sera from acutely-infected pregnant women strongly reacted with rGRA8 in Western-blotting, while sera from chronically-infected or T. gondii-negative women failed to recognize the protein. Conclusion: The full length soluble rGRA8 was successfully produced in E. coli and shown to be a highly immunogenic protein. As a result it could be used in immunological as well as molecular biology experiments.
Toxoplasma gondii, Dense granule antigen, GRA8, Expression, Escherichia coli
145
151
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-32&slc_lang=en&sid=1
2010/04/72010/04/72010/04/7
1389/1/18
2013/03/262013/03/262013/03/26
1392/1/6
Jalal
Babaie
جلال
بابایی
00319475328460056847
00319475328460056847
No
Mehrak
Zare
مهرک
زارع
00319475328460056848
00319475328460056848
No
Ghazaleh
Sadeghiani
غزاله
صادقیانی
00319475328460056849
00319475328460056849
No
Mohammad
Lorgard-Dezfuli
محمد
لرگرد دزفولی
00319475328460056850
00319475328460056850
No
Zohre
Aghighi
زهره
عقیقی
00319475328460056851
00319475328460056851
No
Majid
Golkar
مجید
گلکار
golkar@pasteur.ac.ir
00319475328460056852
00319475328460056852
Yes
en
بررسی کمی تکثیر و تمایز کندروسیت های غضروف مفصلی موش صحرایی در کشت سه بعدی در داخل آلژنیت
Quantitative Analysis of the Proliferation and Differentiation of Rat Articular Chondrocytes in Alginate 3D Culture
Background: While articular chondrocytes are among those appropriate candidates for cartilage regeneration, the cell dedifferentiation during monolayer culture has limited their application. Several investigations have indicated the usefulness of alginate, but the topic of proliferation and differentiation of chondrocytes in alginate culture has still remained controversial. Methods: Rat articular chondrocytes were released by enzymatic digestion, plated at 5 × 104 cells/cm2 and culture-expanded. Passaged-5 cells were then cultivated in alginate as 2-mm beads for a period of two months during which the expansion rate and the level of cell differentiation were determined by [3-(A, 5- dimethylthiazolyl- 2-yl)-1, 5-diphenyl tetrazolium bromide] assay and real-time PCR analysis respectively and compared with those of chondrocytes in monolayer culture. Results: Average population doubling time in alginate cultures (10.04 ± 0.9 days) tended to be significantly (P<0.05) higher than that in monolayer cultures (2.94 ± 0.3 days). The period of alginate culture could be subdivided into expansion phase (Days 0-40) during which proliferation appeared to be high and differentiation phase (Days 40-60) during which the expression of cartilage-specific genes including collagen II and aggrecan tended to be upregulated. During both the proliferation and differentiation phases, the expression of collagen I was low. At chondrocytes monolayer cultures, the proliferating cells appeared to have a very low expression level of cartilage-specific genes and a high expression level of collagen I gene during the entire culture period (P<0.05). Conclusion: It seems that alginate provides conditions in which rat articular chondrocytes are able to undergo proliferation and differentiation in certain time point of cultivation period.
Articular chondrocyte, 3D culture, Monolayer culture, Cell proliferation, Cell structure
153
160
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-30&slc_lang=en&sid=1
2010/04/72010/04/72010/04/72010/04/7
1389/1/18
2013/03/262013/03/262013/03/262013/03/26
1392/1/6
Mohamadreza
Baghaban Eslaminejad
محمدرضا
باغبان اسلامی نژاد
eslami@royaninstitute.org
00319475328460056853
00319475328460056853
Yes
Leila
Taghiyar
لیلا
تقی یار
00319475328460056854
00319475328460056854
No
Fahimeh
Falahi
فهیمه
فلاحی
00319475328460056855
00319475328460056855
No
en
موتاسیون های ژن رسپتور انسولین در بیماران ایرانی مبتلا به دیابت تیپ 2
Insulin Receptor Gene Mutations in Iranian Patients with Type II Diabetes Mellitus
Background: Patients with diabetes mellitus type II suffer from hyperglycemia because they are not able to use the insulin that they produce, often due to inadequate function of insulin receptors. There are some evidences that this deficiency is inherited in a dominant autosomal manner and leads to the malfunction of the pancreatic beta cells resulting in insulin excretion disorders. In this study, we sought to identify mutations in the insulin receptor (INSR) gene, which can cause insulin resistance in type II diabetic patients. Methods: DNA was extracted from peripheral blood cells of the patients (n = 128) diagnosed with type II diabetes. All 22 exons of the INSR gene of the patients were analyzed for mutations running PCR, conformation-sensitive gel electrophoresis and DNA sequencing, consecutively. Results: Approximately 26% of the patients had genetic mutations however, most of them were not reported. These mutations include exon 2 (His171Asn, Ile172Ser, Cys196Ser and Ser210Arg), exon 3 (Gly227Asp and Gly232Ser), exon 8 (Thr543Ser), exon 9 (a heterozygote was observed with no change in phenylalanine at position 669), exon 13 (two heterozygotes: Arg890Pro with Asn865 remaining unchanged), exon 14 (Ala906Gly and Pro918Trp with Arg902 unchanged), exon 17 (Val1086Glu) and exon 19 (His1157Gln with Thr1172 unchanged). Conclusion: The lack of similar mutation records in literature and genetic data banks may suggest a geographic pattern for these INSR gene variants in our population.
Diabetes type II, Insulin resistance, PCR, Conformation-sensitive gel electrophoresis (CSGE), Iran
161
168
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-33&slc_lang=en&sid=1
2010/04/72010/04/72010/04/72010/04/72010/04/7
1389/1/18
2013/03/262013/03/262013/03/262013/03/262013/03/26
1392/1/6
Bahram
Kazemi
بهرام
کاظمی
bahram_14@yahoo.com
00319475328460056856
00319475328460056856
Yes
Negar
Seyed
نگار
سید
00319475328460056857
00319475328460056857
No
Elham
Moslemi
الهام
مسلمی
00319475328460056858
00319475328460056858
No
Mojgan
Bandehpour
مژگان
بنده پور
00319475328460056859
00319475328460056859
No
Maryam
Bikhof Torbati
مریم
بیخاف تربتی
00319475328460056860
00319475328460056860
No
Navid
Saadat
نوید
سعادت
00319475328460056861
00319475328460056861
No
Akram
Eidi
اکرم
عیدی
00319475328460056862
00319475328460056862
No
Elham
Ghayoor
الهام
غیور
00319475328460056863
00319475328460056863
No
Fereydoun
Azizi
فریدون
عزیزی
00319475328460056864
00319475328460056864
No
en
کشت همزمان سلول های فیبروبلاست و کراتینوسیت پوست انسان بر روی داربست زیست سازگار و زیست تخریب پذیر در شرایط آزمایشگاهی
In vitro Co-Culture of Human Skin Keratinocytes and Fibroblasts on a Biocompatible and Biodegradable Scaffold
Background: Extensive full-thickness burns require replacement of both epidermis and dermis. In designing skin replacements, the goal has been to re-create this model and make a product which has both essential components. Methods: In the present study, we developed procedures for establishing confluent, stratified layers of cultured human keratinocytes on the surface of modified collagen-chitosan scaffold that contains fibroblasts. The culture methods for propagation of keratinocytes and fibroblasts isolated from human neonatal foreskin were developed. The growth and proliferation of normal human keratinocytes were evaluated in serum-free (keratinocyte growth medium) and our modified medium. Characterization of human keratinocytes was determined by using pan-keratin and anti-involucrin monoclonal antibodies. For fabrication of relevant biodegradable and biocompatible collagen-chitosan porous scaffold with improved biostability, modified method of freeze-gelation was used. In generating organotypic co-cultures, epidermal keratinocytes were plated onto the upper surface of scaffold containing embedded fibroblasts. Results: The results showed that the growth of isolated human skin fibroblasts and keratinocytes in our modified medium was more than that in the serum-free medium. The different evaluations of collagen-chitosan scaffold showed that it is relevant to growth of cells (fibroblast and keratinocyte) and has a good flexibility in manipulation of the living skin equivalents. Conclusion: These findings indicate that the integration of collagen-chitosan scaffold with co-cultured keratinocyte and fibroblast in vitro provides a potential source of living skin for grafting in vivo.
Co-culture, Keratinocyte, Scaffold, Human skin
169
177
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-35&slc_lang=en&sid=1
2010/04/72010/04/72010/04/72010/04/72010/04/72010/04/7
1389/1/18
2013/03/262013/03/262013/03/262013/03/262013/03/262013/03/26
1392/1/6
Seyed Ramin
Pajoum Shariati
سید رامین
پژوم شریعتی
00319475328460056865
00319475328460056865
No
Mohammad Ali
Shokrgozar
محمدعلی
شکرگزار
mashokrgozar@pasteur.ac.ir
00319475328460056866
00319475328460056866
Yes
Manouchehr
Vossoughi
منوچهر
وثوقی
00319475328460056867
00319475328460056867
No
Ali
Eslamifar
علی
اسلامی فر
00319475328460056868
00319475328460056868
No
en
پلاسمای لخته شده انسانی به عنوان یک ماتریکس کم هزینه و طبیعی جهت آنژیوژنز برون تنی
Human Coagulated Plasma as a Natural and Low Cost Matrix for in vitro Angiogenesis
Background: Angiogenesis, the development of new blood vessels, is an important process in tissue development and wound healing, but becomes pathologic when associated with solid tumor growth, proliferative retinopathies, and rheumatoid arthritis. Accurate and reliable qualification of neovascular (angiogenic) response, both in vitro and in vivo, is an essential requirement for the study of new blood vessel growth. The complexity of currently used three-dimensional in vitro angiogenesis systems makes it difficult to approve material in its models. Capillary-like structure occurs on basement membrane components such as collagen and/or laminin, while in other models, CLS formation occurs on transitional matrices such as fibrin. To solve this problem, we were interested in developing an angiogenesis system which allows rapid and reliable quantification of three-dimensional neovessel formation in vitro. Methods: Human bone marrow endothelial cells were seeded on gelatin-coated microcarriers and suspended in a solution of platelet-poor plasma which was induced to polymerize by addition of calcium chloride. In this way, microcarriers were entrapped in three-dimensional coagulated plasma. Results: Within a few hours, endothelial cells begin to leave these supporting microcarries and migrate into the coagulated-plasma matrix and formed CLS within 48-72 hours. Conclusion: We developed a convenient angiogenesis in vitro system which allows reliable quantification of capillary formation in a three-dimensional environment.
Angiogenesis, Endothelial cells (EC), Human coagulated-plasma, Microcarriers (MC)
179
183
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-34&slc_lang=en&sid=1
2010/04/72010/04/72010/04/72010/04/72010/04/72010/04/72010/04/7
1389/1/18
2013/03/262013/03/262013/03/262013/03/262013/03/262013/03/262013/03/26
1392/1/6
Kamran
Mansouri
کامران
منصوری
kmansouri@kums.ac.ir
00319475328460056869
00319475328460056869
Yes
Ali
Mostafaei
علی
مصطفایی
00319475328460056870
00319475328460056870
No
Manochehr
Mirshahi
منوچهر
میرشاهی
00319475328460056871
00319475328460056871
No
Hamidreza
Mohammadi Motlagh
حمیدرضا
محمدی مطلق
00319475328460056872
00319475328460056872
No
Ali
Maleki
علی
ملکی
00319475328460056873
00319475328460056873
No
Maryam
Keshavarz
مریم
کشاورز
00319475328460056874
00319475328460056874
No
en
تاثیرژن Vhs ویروس هرپس سیمپلکس به عنوان یک ژن جدید خودکشی سلولی بر سلولهای توموری
The Effect of Herpes Simplex Virus Virion Host Shutoff Gene- a New Suicide Gene- on Tumor Cells
Background: The herpes simplex virus (HSV) UL41 gene product, virion host shutoff (Vhs) protein, mediates the rapid degradation of both viral and cellular mRNA. This ability suggests that Vhs protein can be used as a suicide gene in cancer gene therapy applications. The recent reports have shown that the degradation of cellular mRNA during herpes simplex infection is selective. RNA containing AU-rich elements (ARE) in their 3’ untranslated ends are the targets for the Vhs protein. RNA that are not subject to Vhs protein-dependent degradation are up-regulated during HSV infection. ARE are frequently found in mRNA that encode proto-oncogenes, nuclear transcription factors, and cytokines. In many human cancers, the AU-rich stretch of proto-oncogenes and regulatory genes has impaired. Methods: To investigate whether Vhs protein might be useful for inhibition of tumor cell proliferation, a eukaryotic expression vector containing Vhs protein gene was constructed. Cell degradation and RNA content of HeLa and MRC-5 tumor cells after transfection with the constructed vector were studied. Results: The results showed a strong inhibitory activity in proliferation of transfected tumor cells and a sharp decrease in their RNA content. Conclusion: These data suggest that Vhs protein can be considered as a candidate for suicide cancer gene therapy.
Herpes simplex virus (HSV), Virion host shut off (Vhs), Tumor, RNA degradation
185
189
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-31&slc_lang=en&sid=1
2010/04/72010/04/72010/04/72010/04/72010/04/72010/04/72010/04/72010/04/7
1389/1/18
2013/03/262013/03/262013/03/262013/03/262013/03/262013/03/262013/03/262013/03/26
1392/1/6
Hossei
Bannazadeh Baghi
حسین
بنازاده باقی
00319475328460056875
00319475328460056875
No
Taravat
Bamdad
طراوت
بامداد
bamdad_T@modares.ac.ir
00319475328460056876
00319475328460056876
Yes
Hoorieh
Soleimanjahi
حوریه
سلیمانجاهی
00319475328460056877
00319475328460056877
No