en
jalali
1380
12
1
gregorian
2002
3
1
6
2
online
1
fulltext
en
Construction of Hybrid Gene of Hepatitis B Surface Antigen Carrying Heat-Stable Enterotoxin of Escherichia coli and Its Expression in Mammalian Cell Line
Construction of Hybrid Gene of Hepatitis B Surface Antigen Carrying Heat-Stable Enterotoxin of Escherichia coli and Its Expression in Mammalian Cell Line
Hepatitis B surface antigen is the first genetically engineered vaccine licensed for human use. Various strategies have been proposed to obtain a vaccine that would bypass the need for injection. In this study, a non-toxic portion of heat-stable enterotoxin of Escherichia coli that is capable of adhering to epithelial cells was inserted at amino acid position 112 of hepatitis surface antigen. The construct was used for transfection of human embryonic kidney cells in order to assess the expression of the hybrid protein. The data obtained showed a very low level of expression. In vivo antibody production and cytotoxic T lymphocyte response in B6 mice were assessed using DNA immunization. Three out of five injected mice responded with titers >10 mIU/ml anti-HBsAg and cytotoxic T-lymphocyte response was much higher with construct encoding the chimeric protein. Although this study proves that the chimeric protein is capable of eliciting both humoral and cellular responses, but further work is required to fully explore the feasibility of combining the properties of the two proteins .
Chimeric protein, Hepatitis B surface antigen, Heat stable enterotoxin
47
53
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-295&slc_lang=en&sid=1
2011/12/11
1390/9/20
2002/03/15
1380/12/24
Nader
Shahrokhi
نادر
شاهرخی
00319475328460057758
00319475328460057758
No
Anis
Jafari
انیس
جعفری
00319475328460057759
00319475328460057759
No
Valery V
Bakayev
Jorg
Reimann
00319475328460057760
00319475328460057760
No
Reinhold
Schirmbeck
Reinhold
Schirmbeck
00319475328460057761
00319475328460057761
No
Jorg
Reimann
Valery V
Bakayev
00319475328460057762
00319475328460057762
No
Saeid
Bouzari
سعید
بوذری
00319475328460057763
00319475328460057763
Yes
en
The Extractability of Inner-Membrane Proteins from Salmonella typhimurium Intact Cells, Spheroplasts and Inner-Membrane Fragments by Non-Denaturing Detergents
The Extractability of Inner-Membrane Proteins from Salmonella typhimurium Intact Cells, Spheroplasts and Inner-Membrane Fragments by Non-Denaturing Detergents
The effect of Triton X-100, Na cholate and Tween 80 on the solubilization of integral membrane proteins in intact cells, spheroplasts and inner-membrane fragments of Salmonella typhimurium was studied. The detergents were used in various concentrations (1.6 to 64 mM) and cytochromes b and d were used as marker to monitor the solubilization of membrane-bound proteins. Results showed that no inner-membrane protein solubilization was detected after the treatment of intact cells with detergents. The effect of Na cholate and Tween 80 on spheroplasts and inner-membrane fragments was also negligible in comparison to Triton X-100. Triton X-100 solubilized cytochromes from inner-membrane fragments more efficiently than from spheroplasts. The ratio of total protein solubilization to solubilize cytochromes showed that in spheroplasts this ratio was maximum at 1.6 mM Triton X-100 while it was maximum at 16-32 mM Triton X-100 in inner-membrane fragments. This difference between spheroplasts and inner-membrane fragments may be due to the orientation of the inner- membrane in spheroplasts (right side out) and inner-membrane fragments (in-side out as well as right side out), and to the presence of peripheral proteins attached to cytoplasmic membrane in spheroplasts
Detergents, Cytochromes, Salmonella typhimurium
55
61
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-296&slc_lang=en&sid=1
2011/12/112011/12/11
1390/9/20
2002/03/152002/03/15
1380/12/24
Dariush
Minai-Tehrani
داریوش
مینایی تهرانی
minae@ibb.ut.ac.ir
00319475328460057764
00319475328460057764
Yes
Ezzatollah
Keyhani
عزت ا...
کیهانی
00319475328460057765
00319475328460057765
No
Zahra
Sobhani-Damavandifar
زهرا
سبحانی دماوندی فر
00319475328460057766
00319475328460057766
No
en
Effect of ATP-Dependent K+ Channel Openers and Blockers on Serum Concentration of Aldosterone in Rats
Effect of ATP-Dependent K+ Channel Openers and Blockers on Serum Concentration of Aldosterone in Rats
There are many reports for involvement of ATP-sensitive potassium channels in pancreatic, cardiac and vascular smooth muscle cells. This study examined the effect of single doses of K+ channel openers diazoxide, minoxidil and K+ channel blockers chlorpropamide, glibenclamide on serum concentration of aldosterone in male rats. Blood samples were obtained 60 minutes after drug treatment and serum aldosterone level was determined by RIA. The basal serum aldosterone was 659.32 ± 71.48 pg/ml and after diazoxide or minoxidil administration increased to 1188.53 ± 99.45 pg/ml and 1392.69 ± 177.83 pg/ml, respectively. Chlorpropamide or glibenclamide treatment did not produce any change in basal serum aldosterone concentration, but in early streptozotocin-induced diabetic rats decreased serum aldosterone level significantly (P<0.001). Pretreatment with glibenclamide blocked aldosterone response to diazoxide but did not affect aldosterone response to exogenous ACTH to the same extent. Effect of diazoxide in insulin-treated rats was approximately similar to that of normal rats. Comparison of blood glucose concentration determined in normal, insulin treated and diabetic rats after different drug administration showed that there is no correlation between blood glucose level and the responses observed in serum hormone concentration. The results indicate that regulatory processes involved in the secretion of aldosterone are responsive to drugs affecting glibenclamide–sensitive K+ channels
ATP–dependent K+ channel, Aldosterone, Blood glucose, ACTH
63
67
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-297&slc_lang=en&sid=1
2011/12/112011/12/112011/12/11
1390/9/20
2002/03/152002/03/152002/03/15
1380/12/24
Ali
Babaei
علی
بابایی
00319475328460057767
00319475328460057767
Yes
Taghi
Ghafghazi
تقی
قفقازی
00319475328460057768
00319475328460057768
No
Mohsen
Ani
محسن
آنی
00319475328460057769
00319475328460057769
No
en
Comparison of Lactic Acid Isomers Produced by Fungal and Bacterial Strains
Comparison of Lactic Acid Isomers Produced by Fungal and Bacterial Strains
Many organisms produce lactic acid by fermentation, but most industrially important strains are from the genus Lactobacillus and Rhizopus oryzae. L(+)-Lactic acid is the only optical isomer for use in pharmaceutical and food industries because human body is only adapted to assimilate this form. In this research, six strains of Lactobacillus and four strains of R. oryzae (known as high producer) were examined for optical isomers of lactic acid. The production of lactic acid was improved and lactic acid produced in submerged media on rotary shaker incubator. The optical isomers of lactic acid were examined by L(+) and D(-) lactate dehydrogenase kit. All the R. oryzae strains tested produced only L(+) isomer of lactic acid. The highest fungal and bacterial producer strains were R. oryzae PTCC 5263, Lactobacillus plantarum PTCC 1058, L. Bulgaricus PTCC 1332 and L. delbruekii subsp delbruekii PTCC 1333. Lactobacilli strains produced combination of both optical isomers of lactic acid. Among them, L. casei subsp. Casei produced the low amount of D(-)-lactic acid (2%). The optimum concentration of glucose for lactic acid production by R. oryzae and Lactobacillus strains were 180 g/l and 80–120 g/l, respectively.
Lactic acid, Production, Optical isomers
69
75
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-298&slc_lang=en&sid=1
2011/12/112011/12/112011/12/112011/12/11
1390/9/20
2002/03/152002/03/152002/03/152002/03/15
1380/12/24
Saeed
Mirdamadi
سعید
میردامادی
Mirdamadi@irost.com
00319475328460057770
00319475328460057770
Yes
Hajar
Sadeghi
هاجر
صادقی
00319475328460057771
00319475328460057771
No
Nora
Sharafi
نورا
شرفی
00319475328460057772
00319475328460057772
No
Masoud
Fallahpour
مسعود
فلاح پور
00319475328460057773
00319475328460057773
No
Farzaneh
Aziz Mohseni
فرزانه
عزیز محسنی
00319475328460057774
00319475328460057774
No
Mohammad Reza
Bakhtiari
محمد رضا
بختیاری
00319475328460057775
00319475328460057775
No
en
The Effect of Estrogen on Endothelial Permeability of Aorta and the Level of Serum Nitrite Concentration in Cholesterol‑Fed Ovariectomized Rabbit
The Effect of Estrogen on Endothelial Permeability of Aorta and the Level of Serum Nitrite Concentration in Cholesterol‑Fed Ovariectomized Rabbit
Estrogen Replacement Therapy (ERT) in postmenopausal women may decrease the risk of Coronary Artery Diseases (CAD). We hypothesized that Nitric Oxide (NO) releasing due to ERT may be the essential factor by which endothelial permeability decreases. Four groups of ovariectomized rabbits were under investigation for five weeks. Groups 1 & 4 received high cholesterol diet and other two groups (2 & 3) had normal diet. Estradiol valerate (5 mg) was injected weekly in groups 1 & 2. Blood samples were taken before and after the experiment. Finally, the animals were sacrificed for endothelial permeability determination and pathological investigation of aortae. After five weeks, the total cholesterol, triglycerides, HDL and LDL were significantly different between high cholesterol‑fed and normal diet groups (P<0.05). In cholesterol-fed groups, triglycerides concentration was also different significantly (P<0.05). Nitrite concentration was increased significantly in group 1, and it was different from other groups (P<0.05). A considerable decrease of aorta permeability was obtained in group 1 but it was not significantly different from group 4 (P<0.1). The considerable existence of fatty streaks was observed in the animals aortae of group 4, and it was significantly different from group 1 (P<0.05). It suggests that prevention of intimal collection of foam cells and fatty streak in aorta by estrogen may be exerted by NO production.
Estrogen, Nitric Oxide, Permeability, Aorta
77
82
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-299&slc_lang=en&sid=1
2011/12/112011/12/112011/12/112011/12/112011/12/11
1390/9/20
2002/03/152002/03/152002/03/152002/03/152002/03/15
1380/12/24
Mehdi
Nematbakhsh
مهدی
نعمت بخش
00319475328460057776
00319475328460057776
Yes
Parichehr
Hayat‑Davoodi
پریچهر
حیات داودی
00319475328460057777
00319475328460057777
No
Parvin
Rajabi
پروین
رجبی
00319475328460057778
00319475328460057778
No
Seyyed‑Hossein
Samarian
سیدحسین
ثمریان
00319475328460057779
00319475328460057779
No
en
Function of Neutrophils in Different Phases of Chronic Myelogenous Leukemia
Function of Neutrophils in Different Phases of Chronic Myelogenous Leukemia
In chronic myelogenous leukemia (CML), the mature granulocytes originate from a stem cell line harboring an abnormal chromosome, therefore it is possible that metabolic-functional abnormalities occur in the morphologically mature cells. In the present study, the phagocytic activity including intracellular killing, nitro blue tetrazolium (NBT) reduction, and phagocytosis were studied in 37 CML patients in different stages of the disease. The results were compared with those of 37 normal controls. Patients’ neutrophils display significantly lower intracellular killing (P<0.01), NBT reduction (P<0.01) and phagocytosis (P<0.001) than that of normal controls. Analysis of the results revealed an inverse correlation between phagocytic activity and leukocyte count or percentage of immature cells (r = -0.3, P<0.01). In conclusion, the results indicate that neutrophils of CML patients have impaired phagocytic activity. This defect is more prominent in patients in blastic phase, whereas patients in remission show normal values.
chronic myelogenous leukemia (CML), neutrophil, phagocytic function
83
88
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-300&slc_lang=en&sid=1
2011/12/112011/12/112011/12/112011/12/112011/12/112011/12/12
1390/9/21
2002/03/152002/03/152002/03/152002/03/152002/03/152002/03/15
1380/12/24
Soheila
Ajdary
سهیلا
اژدری
ajdsoh@institute.pasteur.ac.ir
00319475328460057780
00319475328460057780
Yes
Latif
Hamidpoor
لطیف
حمیدپور
00319475328460057781
00319475328460057781
No
Tahereh
Zandieh
طاهره
زندیه
00319475328460057782
00319475328460057782
No
en
Endometrial Granulated Lymphocytes in Women Suffering Spontaneous Early Pregnancy Loss
Endometrial Granulated Lymphocytes in Women Suffering Spontaneous Early Pregnancy Loss
Spontaneous abortion is the most common complication of pregnancy. Several lines of evidence indicate that immunologic effector cells may play a role in the pathogenesis of idiopathic repetitive abortions. Leukocytes form a substantial proportion of stromal cells in decidua and the endometrial granulated lymphocytes (eGL) is the predominant decidual leukocyte population in the first trimester of normal human pregnancy. To investigate the involvement of eGL population in repetitive abortion of unknown etiology, a comparative analysis was performed on first-trimester decidual tissues obtained from thirty patients with recurrent spontaneous abortion and thirty samples at therapeutic abortion. The eGL in paraffin-embedded sections of all samples were demonstrated with phloxine-tartrazine staining. The results showed the presence of many eGL that scattered individually throughout the stroma and formed some aggregates around glands and some vessels. The number of positive cells was increased in the recurrent aborted decidua compared with normal pregnancy decidua, but the difference was not significant (p>0.05).
Spontaneous abortion, Endometrial granulated lymphocytes, First-trimester decidua, Phloxine-tartrazine stain
89
92
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-301&slc_lang=en&sid=1
2011/12/112011/12/112011/12/112011/12/112011/12/112011/12/122011/12/12
1390/9/21
2002/03/152002/03/152002/03/152002/03/152002/03/152002/03/152002/03/15
1380/12/24
Mehri
Ghafourian Boroujerdnia
مهری
غفوریان بروجردنیا
00319475328460057783
00319475328460057783
Yes
Rahim
Chinipardaz
رحیم
چینی پرداز
00319475328460057784
00319475328460057784
No
en
Isolation of a Penicillin Acylase Producing E.coli and Kinetic Characterization of the Whole Cell Enzyme Activity
Isolation of a Penicillin Acylase Producing E.coli and Kinetic Characterization of the Whole Cell Enzyme Activity
Penicillin acylase (EC 3.5.1.11) has been a target of study for a long time because of its pivotal role in the deacylation of the penicillin into the 6- aminopenicillanic acid (6-APA) and the side-chain organic acids. This property of penicillin acylase has been exploited commercially for large scale production of 6-APA, which is the key intermediate in the manufacture of semi-synthetic penicillins. Due to the worldwide demand for semi-synthetic penicillins, production of 6-APA has been increased up to 7000 tons in recent years. In this study, Sixty-five strains of E. coli were investigated for penicillin acylase activity using fluorescamine method. The 6-aminopenicillanic acid formed in the reaction mixture was developed on thin layer chromatography. One-minute beta-lactamase test was carried out to follow any trace of penicillinase activity. Only one sample designated as E.coli PPA78 was found to be penicillin acylase producer. The optimal pH and temperature of penicillin acylase activity of the whole cells were determined to be 8.0 and 57° C, respectively. Km value and activation energy of the enzymatic hydrolysis reaction of penicillin G by intracellular enzyme were estimated as 0.004 mmol and 6.2 Kcal/mol, respectively.
penicillin acylase, E. coli, Fluorescamine,
93
96
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-302&slc_lang=en&sid=1
2011/12/112011/12/112011/12/112011/12/112011/12/112011/12/122011/12/122011/12/12
1390/9/21
2002/03/152002/03/152002/03/152002/03/152002/03/152002/03/152002/03/152002/03/15
1380/12/24
Sedigheh
Javadpour
صدیقه
جوادپور
00319475328460057785
00319475328460057785
No
Dariush
Norouzian
داریوش
نوروزیان
dnsa@institute.pasteur.ac.ir
00319475328460057786
00319475328460057786
Yes
Azim
Akbarzadeh
عظیم
اکبرزاده
00319475328460057787
00319475328460057787
No
Saeed
Mirdamadi
سعید
میردامادی
00319475328460057788
00319475328460057788
No
Behrokh
farahmand
بهرخ
فرهمند
00319475328460057789
00319475328460057789
No