en
jalali
1381
10
1
gregorian
2003
1
1
7
1
online
1
fulltext
en
rDNA-ITS2 Identification of Anopheles pulcherrimus (Diptera: Culicidae): Genetic Differences and Phylogenetic Relation with Other Iranian Vectors and Its Implications for Malaria Control
rDNA-ITS2 Identification of Anopheles pulcherrimus (Diptera: Culicidae): Genetic Differences and Phylogenetic Relation with Other Iranian Vectors and Its Implications for Malaria Control
Anopheles pulcherrimus Theobald has a wide distribution in western Asia and is a potential vector of malaria in Iran. We have examined the rDNA-ITS2 (internal transcribed spacer 2) region of An. pulcherrimus specimens collected during the two peaks of activity (May-June and October-November) from Sistan and Baluchistan province, southeastern Iran. There were no consistent differences between specimens originated from different ecological areas. Total amplified fragment is 490 bp, which is within the range of the records repeated from other anophelines. ITS2 was 350 bp long in all individuals examined with identical sequence in different populations. Sequence analysis revealed its differences with two other important malaria vectors in the region, An. culicifacies and An. fluviatilis. However, based on ITS2-derived phylogenetic tree, the nearest taxa to An. pulcherrimus is a new species related to An. Culicifacies and called species X in An. culicifacies species complex. These data may provide a better understanding on dynamics of malaria transmission in southeastern corner of Iran and neighboring countries. Moreover, the extent of the genetic variation in these mainly sympatric species could result in designing and application of species-specific diagnostic tools, which can facilitate the management of malaria control program in the region
Anopheles pulcherrimus, rDNA-ITS2, Molecular identification
1
6
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-279&slc_lang=en&sid=1
2011/12/10
1390/9/19
2003/01/15
1381/10/25
Navid
Dinparast Djadid
نوید
دین پرست جدید
navid@institute.pasteur.ac.ir
00319475328460057697
00319475328460057697
Yes
Mohammad Hossein
Sanati
محمد حسین
صنعتی
00319475328460057698
00319475328460057698
No
Mohammad
Zare
محمد
زارع
00319475328460057699
00319475328460057699
No
Abdolghaffar
Hassanzehi
عبدالغفار
حسن زهی
00319475328460057700
00319475328460057700
No
en
Ultrastructural Study of Rotavirus Replication and Localization of the Intermediate Capsid Protein VP6
Ultrastructural Study of Rotavirus Replication and Localization of the Intermediate Capsid Protein VP6
Rotavirus, a triple-layered non-enveloped member of the Reoviridae family, obtained a transient membrane envelope when newly synthesized subviral particles bud into the endoplasmic reticulum (ER). As rotavirus particles mature, they lose their transient membrane and obtain outer layer. It is mostly believed that only double layered particles bud into the ER. The present study describes that the single layered particles can also bud into the ER and become the immediate precursors of the mature virions. Virus replication was studied within a line of African green monkey kidney (BSC-1) cells infected with simian rotavirus SA11. The virus intermediate capsid protein (VP6) was localized within the infected cells using protein A-gold. Monospecific antibody to VP6 was the primary antibody. The electron micrographs of budding sites of ER showed two different sizes of subviral particles. The gold particles were seen on the double layered particles and very little in the cytoplasm and some on ER or very close to it. These results indicate that the single layered particles are also capable of being the precursor of the triple layered and obtain the VP6 while budding into the ER
Rotavirus, Morphogenesis, Immunoelectron microscopy
7
11
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-280&slc_lang=en&sid=1
2011/12/102011/12/10
1390/9/19
2003/01/152003/01/15
1381/10/25
Shahin
Ahmadian
شهین
احمدیان
Ahmadian@ibb.ut.ac.ir
00319475328460057701
00319475328460057701
Yes
en
Introduction of Three Independent Selection Markers in Leishmania
Introduction of Three Independent Selection Markers in Leishmania
The pLE2SCX vector was developed for the stable expression of exogenous genes in the protozoan parasite Leishmania. The pLE2SCX construct contains three independent selection markers: herpes simplex virus thymidine kinase (HSV-TK), cytosine deaminase (CD) and streptothericin acetyltransferase gene (sat) in multiple cloning site, flanking by 5’ and 3’ untranslated regions of the previously cloned Leishmania major hexa-binding protein gene. Selection was based on resistance to the nourseothericin (Ns) which corresponds to sat gene. The two negative selection HSV-TK and CD genes, make the transformed cell sensitive to ganciclovir (GCV) and 5-fluorocytosine (5-FC). The vector was introduced into Leishmania promastigotes by electroporation and maintained as circular form. The selected transfectants were not grown on media with GCV or 5-FC. Using two drug sensitive selectable markers together on a vector is a novel strategy in gene cloning in Leishmania. This stable transfection vector has allowed the permanently expression of several different exogenous genes at the same time in Leishmania
HSV-thymidine kinase (HSV-TK), Cytosine deaminase (CD), ganciclovir (GCV), 5-fluorocytosine (5-FC), nourseothericin (Ns)
13
18
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-281&slc_lang=en&sid=1
2011/12/102011/12/102011/12/10
1390/9/19
2003/01/152003/01/152003/01/15
1381/10/25
Noushin
Davoudi
نوشین
داوودی
00319475328460057702
00319475328460057702
No
Fereidoun
Mahboudi
فریدون
مهبودی
00319475328460057703
00319475328460057703
Yes
Mohammad
Azizi
محمد
عزیزی
00319475328460057704
00319475328460057704
No
Ahmad
Adeli
احمد
عادلی
00319475328460057705
00319475328460057705
No
Robert. W
McMaster
Robert. W
McMaster
00319475328460057706
00319475328460057706
No
en
Lectin-Binding Patterns in the Microenvironment of the Mouse Developing T-Cells
Lectin-Binding Patterns in the Microenvironment of the Mouse Developing T-Cells
Glycoconjugates and their programmed changes during the course of development in the cell-surface as well as in the extracellular matrix, are known to affect cell differentiation, cellular interaction and other developmental phenomena during embryogenesis. The purpose of this study was to localize N-acetylgalactosamin as well as fucose-containing glycoconjugates in situ during thymus development. Staged embryos or thoracic segments were fixed and processed for lectin histochemistry studies. Five microns paraffin-embedded sections were incubated with horseradish peroxidase conjugated lectins from Dolichos biflorus and Aleuria aurantia specific for N-acetylgalactosamine and fucose, respectively. Our results revealed unique reaction of T-cells with Dolichos biflorus and the presence of a fucosylated glycoconjugate in microenvironments of the developing thymus including extracellular matrix and developing Hassall bodies. The time and distribution of staining with these two lectins suggest that fucosylated glycoconjugates and N-acetylgalactosamin terminal sugars may play significant role in intrathymic microenvironment that might cause differentiation of T-lymphocytes
Lectin histochemistry, Thymus, T-lymphocytes, Extracellular matrix
19
22
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-282&slc_lang=en&sid=1
2011/12/102011/12/102011/12/102011/12/10
1390/9/19
2003/01/152003/01/152003/01/152003/01/15
1381/10/25
Fateme Cobra
Ganji
فاطمه کبری
گنجی
00319475328460057707
00319475328460057707
Yes
Ali Reza
Fazel
علی رضا
فاضل
00319475328460057708
00319475328460057708
No
en
Effect of Aqueous Garlic (Allium sativum L.) Extract on Acetylcholine and Isosorbide-Induced Relaxation of Isolated Aorta in Rat
Effect of Aqueous Garlic (Allium sativum L.) Extract on Acetylcholine and Isosorbide-Induced Relaxation of Isolated Aorta in Rat
The hypotensive effect of garlic has been well-documented in human subjects and animals. Since endothelial activity regulates vascular reactivity in physiological and pathophysiological conditions, the aim of the present study was to investigate the effect of garlic on endothelium-dependent and independent relaxation of rat aorta for elucidation of mechanism of the garlic anti-hypertensive effect. Four and eight weeks after treatment with garlic extract, aortic rings were studied for relaxation response to acetylcholine and isosorbide dinitrate. The obtained results showed that endothelium-dependent relaxation response of aortic rings to acetylcholine, from rats treated with garlic for 4 and 8 weeks, increases about 5-24% and 3-27%, respectively compared to controls. But, endothelium-independent relaxation response to isosorbide showed no difference in all groups. Moreover, the relaxant effect of garlic extract was time-dependent. The obtained findings strongly suggest that garlic exerts its relaxant effect through endothelium by production and/or releasing of endothelium-derived relaxing factor
Aqueous garlic extract, Aortic rings, Acetylcholine, Isosorbide dinitrate
23
27
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-283&slc_lang=en&sid=1
2011/12/102011/12/102011/12/102011/12/102011/12/10
1390/9/19
2003/01/152003/01/152003/01/152003/01/152003/01/15
1381/10/25
Homayoun
Homayounfar
همایون
همایونفر
Homayoun@iums.ac.ir
00319475328460057709
00319475328460057709
Yes
Tourandokht
Baluchnejad Mojarad
توراندخت
بلوچ نژاد مجرد
00319475328460057710
00319475328460057710
No
Mehrdad
Roghani
مهرداد
روغنی
00319475328460057711
00319475328460057711
No
Mahmoud
Hosseini
محمود
حسینی
00319475328460057712
00319475328460057712
No
Mohammad
Kamalinejad
محمد
کمالی نژاد
00319475328460057713
00319475328460057713
No
en
Screening of Lovastatin Production by Filamentous Fungi
Screening of Lovastatin Production by Filamentous Fungi
In the present study, 110 fungal strains of Persian Type Culture Collection (PTCC) including some selected strains isolated in various screening projects were tested for their potentiality to produce lovastatin, a competitive inhibitor of 3-hydroxy-3-methylglutaryl-CoA reductase (HMG-CoA reduc-tase), the rate-limiting enzyme of cholesterol biosynthesis. The fungal strains were cultivated in a two-stage submerged fermentation followed by screening by TLC. All positive results were evaluated by confirmatory HPLC. Nine species of four genera were found to be lovastatin producers. Aspergillus terreus was the best lovastatin producing strain with a level of 55 mg lovastatin per liter of screening production medium
Lovastatin, Screening, Fungal strains, Aspergillus terreus
29
33
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-284&slc_lang=en&sid=1
2011/12/102011/12/102011/12/102011/12/102011/12/102011/12/10
1390/9/19
2003/01/152003/01/152003/01/152003/01/152003/01/152003/01/15
1381/10/25
Siamak M
Samiee
سیامک
سمیعی
smsamiee@irost.com
00319475328460057714
00319475328460057714
Yes
Nasrin
Moazami
نسرین
معظمی
00319475328460057715
00319475328460057715
No
Saeid
Haghighi
سعید
حقیقی
00319475328460057716
00319475328460057716
No
Farzaneh
Aziz Mohseni
فرزانه
عزیز محسنی
00319475328460057717
00319475328460057717
No
Saeid
Mirdamadi
سعید
میردامادی
00319475328460057718
00319475328460057718
No
Mohammad Reza
Bakhtiari
محمدرضا
بختیاری
00319475328460057719
00319475328460057719
No
en
Cockroaches as Reservoirs and Vectors of Drug Resistant Salmonella spp.
Cockroaches as Reservoirs and Vectors of Drug Resistant Salmonella spp.
Cockroaches were collected from hospitals, houses and poultry sheds in various parts of Isfahan (Iran) and identified to species. In total, seven species of cockroaches in seven genera were identified: Blatta lateralis, Polyphaga aegyptiaca, Arenivaga roseni and Parcoblatta spp. Three species Periplaneta americana, Blattella germanica and Supella longipalpa (Blattidae and Blattellidae) were more abundant than the others. In another study, forty cockroaches were collected from hospitals in two experiments and were studied for the presence of Salmonella spp. Salmonella (Black colonies on SS agar with urease negative) were isolated from about 70 percent of the cockroaches collected from hospitals. Some of the isolated Salmonella were resistant to antibacterial drugs in a susceptibility test. Isolation of Salmonella from cockroaches collected from hospitals suggests that cockroaches act as natural reservoirs of Salmonella. A second study was conducted to determine if individual B. germanica could transfer Salmonella from an infected food source and then infects uncontaminated colony members. The results showed that the inoculation of 106 CFU of Salmonella into cockroaches via their food could infect the uncontaminated cockroaches. These contaminated cockroaches transfer infection to other colony members. Salmonella is stable in cockroaches for more than 10 months.
Salmonella, Cockroach, Drug resistant, Vector
35
38
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-310&slc_lang=en&sid=1
2011/12/102011/12/102011/12/102011/12/102011/12/102011/12/102011/12/29
1390/10/8
2003/01/152003/01/152003/01/152003/01/152003/01/152003/01/152003/01/15
1381/10/25
Hossein
Fathpour
حسین
فاتح پور
00319475328460057720
00319475328460057720
No
Giti
Emtiazi
گیتی
امتیازی
00319475328460057721
00319475328460057721
Yes
Elham
Ghasemi
الهام
قاسمی
00319475328460057722
00319475328460057722
No
en
Antibacterial Effects of Enoxolone on Periodontopathogenic and Capnophilic Bacteria Isolated from Specimens of Periodontitis Patients
Antibacterial Effects of Enoxolone on Periodontopathogenic and Capnophilic Bacteria Isolated from Specimens of Periodontitis Patients
Enoxolone is a major component of a traditional plant called Licorice. This substance has been found to contain some pharmaceutical properties including of both antiviral and antifungal activities. Microbiological studies have identified more than seven periodontopathogens in the periodontal pockets, which less than 4 species were capnophile. The purpose of this study was to investigate the in vitro antibacterial effects of enoxolone against isolated periodontopathogenic and capnophilic bacteria. Total specimens were collected with sterile paper points from the deepest periodontal pockets of 400 patients, and cultured under capnophilic condition using selective media. The isolates were characterized to species level by conventional biochemical tests. Antibacterial activities of enoxolone against those microorganisms were investigated by determining minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) methods. In this study, 186 species of Actinobacillus actinomycetemcomitans (46.5%), 120 species of Eikenella corrodens (30%) and 136 Capnocytophaga species (34%) were isolated from specimens of periodontitis patients. The rate of periodontitis specimens associated with monobacteria and polybacteria were 105 (26.3%) and 158 (39.5%), respectively. The MIC of enoxolone was 8, 16 and 8 mg/ml for A. actinomycetemcomitans, E. corrodens and Capnocytophaga species, respectively and the MBC was 16 mg/ml for all species. It is concluded that enoxolone with above mentioned concentration is effective against isolated periodontopathogenic and capnophilic bacteria.
Antibacteria, Enoxolone, Periodontopathogen, Capnophile, Periodontitis
39
42
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-286&slc_lang=en&sid=1
2011/12/102011/12/102011/12/102011/12/102011/12/102011/12/102011/12/292011/12/10
1390/9/19
2003/01/152003/01/152003/01/152003/01/152003/01/152003/01/152003/01/152003/01/15
1381/10/25
Mohammad Hossein
Salari
محمد حسین
سالاری
msalari2000@yahoo.com
00319475328460057723
00319475328460057723
Yes
Nasralah
Sohrabi
نصرالله
سهرابی
00319475328460057724
00319475328460057724
No
Zainab
Kadkhoda
زینب
کدخدا
00319475328460057725
00319475328460057725
No
Mohammad Bagher
Khalili
محمد باقر
خلیلی
00319475328460057726
00319475328460057726
No