en
jalali
1382
7
1
gregorian
2003
10
1
7
4
online
1
fulltext
en
تاثیرات کمکی ردههای سلولی فیبروبلاست جنینی انسان بر روی رشد و تکثیر سلولهای لنفوبلاستوئید ترانسفورم شده با ویروس EB
Supportive Effects of Human Embryonic Fibroblast Cell Lines on Growth and Proliferation of EBV-Transformed Lymphoblastoid Cells
Human diploid fibroblast cells produce a spectrum of necessary growth factors and extracellular matrix (ECM) components essential for growth and proliferation of a variety of other cell types. In this study, the effect of five human embryonic fibroblast cell lines, isolated from liver, lung, skin and foreskin tissues, was investigated. A coculture system analyse was employed to cloning efficiency (CE) and DNA synthesis of a human Epstein-Barr virus (EBV)-transformed lymphoblastoid cell line (LCL) in long- and short-term cultures. The fibroblast cells were used as feeder layer after treatment with mitomycin C. Optimal density of the feeder cells induced 10 to 43 times higher CE than cultures supplemented with conditioned media (CM) or cultures without a feeder layer. The stimulatory effect of the feeder cells was partly associated to their tissue origin, with the lung and liver fibroblasts being the most and least effective feeder cells, respectively. Short-term cultures of LCL cells with feeder cells or their CM resulted in a marginal increase in DNA synthesis and proliferation as evidenced by the index of ³H-thymidine incorporation. Our results demonstrated supportive effects of feeder cells on the LCL growth, which can not be replaced by their CM. These supportive effects were partly associated with cell density and tissue origin of the feeder cells
Embryo, Fibroblast, Feeder cells, EBV-transformed B-cells, Cloning efficiency
147
153
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-255&slc_lang=en&sid=1
2011/12/7
1390/9/16
2003/10/15
1382/7/23
Saeed
Rajabalian
سعید
رجبعلیان
00319475328460057620
00319475328460057620
No
Zahra
Samadi Bahrami
زهرا
صمدی بهرامی
00319475328460057621
00319475328460057621
No
Vahideh
Farahat
وحیده
فراهت
00319475328460057622
00319475328460057622
No
Fazel
Shokri
فاضل
شکری
fazshok@yahoo.com
00319475328460057623
00319475328460057623
Yes
en
حضور ایمونوگلوبولینهای سطح آماستیگوتهای حاصل از زخم L. major به عنوان وسیلهای برای جداسازی مرحله آماستیگوت از موش BALB/C
Presence of Immunoglobulins on the Surface of Lesion-Derived Amastigotes of Leishmania major As a Tool for Isolation of Amastigote Stage from BALB/c Mice
The onset of infections by Leishmania parasites mainly caused by amastigote growth inside the macrophages. One of the important properties of lesion-derived amastigote is thought to be the attachment of various host proteins including immunoglobulins on the surface of amastigote. In this study, the presence of immunoglobulins on the surface of lesion-derived amastigotes was detected by Western blotting using three different peroxidase conjugated anti-heavy chain antibodies and peroxidase conjugated anti-mouse IgG antibody. Then, a new technique was developed for isolation of lesion-derived amastigotes. This technique simply consists of a microbiological plate covered by rabbit anti-mouse immunoglobulins. Overnight incubation of the infected cell suspension isolated from mice lesion on such plate at 4°C, was followed by gentle washing and isolation of the amastigotes. The results showed that the surface of amastigote has covered with different amount of immunoglobulins such as IgG, IgM, and IgA detected by pixel analysis software. With this technique, which was comparable with other techniques, pure amastigote was isolated. This is a simple and reliable method for isolation of real amastigotes from lesion of the infected BALB/c mice
Leishmania major, Lesion-derived amastigote, Immunoglobulins , BALB/c mice
155
160
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-256&slc_lang=en&sid=1
2011/12/72011/12/10
1390/9/19
2003/10/152003/10/15
1382/7/23
Ali
Mirjalili
علی
منجیلی
00319475328460057624
00319475328460057624
No
Mohammad H
Alimohammadian
محمدحسین
علیمحمدیان
mhalimoh@institute.pasteur.ac.ir
00319475328460057625
00319475328460057625
Yes
Rasoul
Madani
رسول
مدنی
00319475328460057626
00319475328460057626
No
en
مطالعه اثرات مداخله در تنظیم غلظت درون سلولی کلسیم بر روی روند التیام زخم در خرگوش
A Study on the Effects of Modulation of Intracellular Calcium on Excisional Wound Healing in Rabbit
An in vitro study on the role of intracellular calcium ions in healing of excisional wound in rabbit was undertaken. We employed two drugs namely, glibenclamide and nitroglycerin that are topically applied in vivo to modulate the activity of intracellular calcium. Our model consisted of a 15 ´ 15 mm excisional wound. Seven groups of New Zealand rabbits were used. The first three groups served as untreated, Vaseline- and lubricating jell vehicle-treated as controls. The remaining groups received topically 0.5 g of nitroglycerine (2 % in Vaseline base) or glibenclamide (1, 2 and 4% in lubricating jell) on the day of excision and continued for 11 days. Using wound surface area measurement, complemented with measurement of the breaking strength and histological assessment, the results showed that inhibition of intracellular calcium ion had a favorable effect on wound healing. The mean wound half-lives and breaking strength were significant and concentration dependently reduced in glibenclamide-treated group. In contrast, in nitroglycerin-treated group the rate of wound healing and breaking strength were increased relative to untreated control and Vaseline treated groups. Histological findings revealed more organized collagen fibers and angiogenesis in nitroglycerin-treated wounds. The present study demonstrated that intracellular calcium ion has an important role on the overall process of wound healing. This information may be utilized in further studies to assess its role in healing of human wounds
Excisional wound, Nitroglycerin, Glibenclamide, Wound surface area, Tensile strength
161
166
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-257&slc_lang=en&sid=1
2011/12/72011/12/102011/12/10
1390/9/19
2003/10/152003/10/152003/10/15
1382/7/23
Mohammad Hassan
Pipelzadeh
محمدحسن
پیپل زاده
00319475328460057627
00319475328460057627
Yes
Mohammad Reza
Pipelzadeh
محمدرضا
پیپل زاده
00319475328460057628
00319475328460057628
No
Popak
Husseinzadeh
پوپک
حسین زاده
00319475328460057629
00319475328460057629
No
en
تغییرات سطح فعالیت آنزیمی آسپارتیک آمینوترانسفراز میتوکندریایی و سیتوسلی در موشهای صحرائی مسموم شده به آلومینیوم
Changes in the Level of Mitochondrial and Cytosolic Aspartate Aminotransferase Activities in Aluminium Intoxified Rat
The activity of aspartate aminotransferase (AST) in human serum has been widely determined as a diagnostic aid in liver disease. In this study, the effect of aluminium on AST isoenzymes in relation to aluminium intoxified patients has been investigated. Using gel filtration chromatography technique with Sephacryl S-300, mitochondrial aminotransferase (m-AST) and cytosolic aminotransferase (c-AST) fractions were separated from rat serum and liver homogenate. The c-AST fraction was eluted with higher mobility than m-AST iso-enzyme. Daily administration of aluminium (1 and 5 mg/kg body weight) for 30 days increased total serum activity of AST by 19% and 72%, respectively. Daily administration of aluminium (10 and 20 mg/kg body weight) for 30 days was also studied. The percentage of elevations was 114% and 86% in comparison to the controls. Following aluminium administration for 45 days, the enzyme activity was elevated to 20% and 60% in comparison to the controls, and administration for 60 days resulted elevation of 35% and 79%. The serum enzyme activity was mostly due to the mitochondrial fraction of AST that was a time and dose dependent
Mitochondria, Cytosolic, Activity of aspartate aminotransferase (AST), Aluminium, Intoxified
167
171
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-258&slc_lang=en&sid=1
2011/12/72011/12/102011/12/102011/12/10
1390/9/19
2003/10/152003/10/152003/10/152003/10/15
1382/7/23
Ali Asghar
Moshtaghie
علی اصغر
مشتاقی
Moshtaghie@PharmD.mui.ac.ir
00319475328460057630
00319475328460057630
Yes
Iraj
Javadi
ایرج
جوادی
00319475328460057631
00319475328460057631
No
Golamreza
Feghhi
غلام رضا
فقهی
00319475328460057632
00319475328460057632
No
en
اثر کیناپریل بر پاسخ انقباضی آئورت موشهای صحرایی دیابتی شده توسط استرپتوزوتوسین
The Effect of Quinapril on the Aortic Contractile Response of Streptozotocin-Diabetic Rats
Angiotensin-converting enzyme (ACE) inhibitors appear to correct many of the abnormalities associated with the vascular dysfunction found in diabetic patients. In this respect, quinapril is a unique ACE inhibitor with multiple protective effects. The present study was carried out to investigate the effect of intraperitoneal administration of quinapril on the aortic reactivity of streptozotocin (STZ)-diabetic rats. For this purpose, male Wistar rats received one injection of streptozotocin (STZ), 60 mg/kg, to induce diabetes. Three days after STZ injection, rats were treated with quinapril (2 mg/kg/day) for 4 weeks, after that aortic reactivity to vasoactive agents were compared with those of untreated diabetic rats or non-diabetic control rats. For this purpose, contractile response to phenylephrine (PE) was obtained from aortic rings. Concentration-response curves from quinapril-treated diabetic rats to PE in the presence and absence of endothelium were attenuated as compared to vehicle-treated diabetics. Therefore, the 4-week treatment of diabetic rats with quinapril could prevent the functional changes in vascular reactivity in diabetic rats
Quinapril, Aortic reactivity, Diabetes mellitus, STZ, Rat
173
177
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-259&slc_lang=en&sid=1
2011/12/72011/12/102011/12/102011/12/102011/12/10
1390/9/19
2003/10/152003/10/152003/10/152003/10/152003/10/15
1382/7/23
Farshad
Roghani Dehkordi
فرشاد
روغنی دهکردی
00319475328460057633
00319475328460057633
No
Mehrdad
Roghani
مهرداد
روغنی
mehjour@yahoo.com
00319475328460057634
00319475328460057634
Yes
Tourandokht
Baluchnejad Mojarad
توراندخت
بلوچ نژاد مجرد
00319475328460057635
00319475328460057635
No
en
اثر وانادیل سولفات بر مقاومت به انسولین القاء شده توسط فروکتوز در موش صحرائی
Effect of Vanadyl Sulfate on Fructose-Induced Insulin Resistance Rat
Insulin resistance syndrome, also referred to as the metabolic syndrome or syndrome X, refers to a constellation of common metabolic and cardiovascular disorders (e.g. obesity, type 2 diabetes mellitus, hypertension, and dyslipidemia), which are all cardiovascular risk factors. Insulin resistance can be induced by fructose-rich diet in rats. We investigated the effect of vanadyl sulfate (0.2 mg/ml in drinking water for 7 days) on glucose, triglyceride, and plasma insulin levels in male Wistar rats that were fed with fructose-rich diets. Control rats were fed with standard chow for 7 days. The animals were divided into three groups: fructose-fed rats, fructose fed-vanadyl sulfate treated rats, and control rats. Fasting plasma glucose levels of the three groups were comparable (p>0.05). Fasting plasma insulin increased in the fructose-fed rats (190 ± 6.3 pM vs. control rats 83.06 ± 3.3 pM, p<0.001), likewise, plasma triglyceride significantly increased in fructose-fed rats (394.0 ± 25.8 vs. control rats 98.63 ± 6.7, p<0001). Vanadyl sulfate treatment prevented the increase in plasma insulin levels in the fructose fed-vanadyl treated rats (78.9 ± 5.1 pM vs. fructose fed-groups, p<0.001). Also vanadyl sulfate treatment significantly decreased plasma triglyceride levels (116.43 ± 6.7 vs. fructose-fed rats, p<0.001). Furthermore, fructose-fed groups had higher fasting insulin resistance index (FIRI: p<0.001) than control rats. In contrast, vanadyl sulfate significantly decreased FIRI in the fructose fed- vanadyl treated groups (p<0.001) compared with fructose- fed animals. These results indicate that administration of low doses of vanadyl sulfate may be advantageous for preservation of the functional characteristics of pancreatic beta cells, probably by improving insulin action and thereby insulin resistance prevention
Insulin resistance, Vanadyl sulfate, Diabetes mellitus, Fasting insulin resistance index (FIRI), Triglyceride
179
182
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-260&slc_lang=en&sid=1
2011/12/72011/12/102011/12/102011/12/102011/12/102011/12/10
1390/9/19
2003/10/152003/10/152003/10/152003/10/152003/10/152003/10/15
1382/7/23
Mehdi
Harati
مهدی
هراتی
mharati@medinews.com
00319475328460057636
00319475328460057636
Yes
Mohsen
Ani
محسن
آنی
00319475328460057637
00319475328460057637
No
Manoochehr
Messripour
منوچهر
مصری پور
00319475328460057638
00319475328460057638
No
en
گانگلیوژنز سمپاتیک و توزیع سه بعدی و گذرای قندهای انتهایی زنجیرههای گلیکوکونجوگیتها
Sympathetic Gangliogenesis and Temporo-Spatial Glycoconjugates’s Terminal Sugars Distribution
Lectin binding histochemistry was performed on the developing sympathetic ganglionic cells to investigate the distribution and density of defined carbohydrate terminals on the cell surface glycoproteins during autonomic system morphogenesis. Sprague-Dauley rat embryos from 9th gestational day to birth were fixed and paraffinized. Serial sections of these specimens were incubated with different lectins, which were conjugated to horse raddish peroxidase (HRP). Diaminobenzidine was used to localize the HRP on the binding sites of lectins to terminal sugars. Among these lectins, soybean agglutinin reacted with migratory neural crest cells in early stages, from 9th to 13th day of gestation. These cells were moving ventrally in relation to the neural tube. In the late stages from days 14th to 21st, orange peel fungus agglutinin reacted with sympathetic migratory cells. Our results suggest that at each stage of sympathetic ganglia development, a specific glycoconjugate seems to be the key factor for development of the cells. We suggest that only one key specific terminal sugar is active in each stage of development, which might genetically regulated for particular stage of sympathetic gangliogenesis
Neural crest, Sympathetic ganglia, Lectin histochemistry
183
186
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-261&slc_lang=en&sid=1
2011/12/72011/12/102011/12/102011/12/102011/12/102011/12/102011/12/10
1390/9/19
2003/10/152003/10/152003/10/152003/10/152003/10/152003/10/152003/10/15
1382/7/23
Mokhtar
Jafarpur
مختار
جعفرپور
jafarpurmokhtar@yahoo.com
00319475328460057639
00319475328460057639
Yes
Hasan
Mofidpur
حسن
مفیدپور
00319475328460057640
00319475328460057640
No
Alireza
Fazel
علی رضا
فاضل
00319475328460057641
00319475328460057641
No
en
مقایسه تولید میسلیوم توسط کاندیدا آلبیکنس جدا شده از منابع مختلف
Comparison of Mycelial Production by Candida Albicans Isolated from Different Sources
The aim of this study was to compare the ability of 46 isolates of Candida albicans to produce mycelial form with oral source in three groups of individuals including, removable appliance wearers, non-wearers and oral medicine patients. Saliva samples were obtained from all subjects along with a foam imprint from the fitting surface of the upper removable appliance in the case of patients. Colonies that were green-blue were confirmed as C. albicans by the germ-tube test. The production of mycelia was measured in vitro using defined medium (Lee’s medium) during 24 h. The results indicate that the production of mycelia in C. albicans isolated from removable appliance wearers and oral medicine patients are significantly different (p<0.025).
Candida albicans, Mycelial form, Lee’s medium
187
189
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-262&slc_lang=en&sid=1
2011/12/72011/12/102011/12/102011/12/102011/12/102011/12/102011/12/102011/12/10
1390/9/19
2003/10/152003/10/152003/10/152003/10/152003/10/152003/10/152003/10/152003/10/15
1382/7/23
Ali
Zarei Mahmoudabadi
علی
زارعی محمود آبادی
zarei40@hotmail.com
00319475328460057642
00319475328460057642
Yes
David B.
Drucker
David B.
Drucker
00319475328460057643
00319475328460057643
No