en
jalali
1383
7
1
gregorian
2004
10
1
8
4
online
1
fulltext
en
آنالیز توالی نوکلئوتیدی و مطالعه فیلوژنی هماگلوتینین تحت واحد H9N2 ویروس آنفلوانزای طیور جدا شده طی سالهای 81-1378 در ایران
Sequence Analysis and Phylogenetic Study of Hemagglutinin Gene of H9N2 Subtype of Avian Influenza Virus Isolated during 1998-2002 in Iran
Sequence analysis and phylogenetic study of hemagglutinin (HA) gene of H9N2 subtype of avian influenza virus isolates (outbreaks of 1998-2002) in Tehran province (Iran) were studied. Two sets of forward and reverse primers in highly conserved regions, based on sequences of HA gene in Genbank, were designed. PCR products of a 430-bp fragment of 16 isolates were sequenced and then were aligned with the reported sequences in Genbank. Nucleotide sequence comparisons of HA gene from Iranian isolates showed 97-99% identity within the group, and 98% homology with the two isolates [A/Parakeet/Narita/92A/98 (H9N2)] and [A/Parakeet/Chiba/1/97 (H9N2)] from Pakistani parakeets imported to Japan. On the basis of phylogenetic evidence, it is proposed that the emergence of H9N2 avian influenza infection in Iran originated in Pakistan, and it was due to low quarantine measures in the international boundaries. Due to the high percentage of H9N2 homology isolates of Iran with other isolates, namley A/quail/HongKong/G1, in Genbank and based on published reports for high similarity with infecting human H5N1 isolates, it seems that the potential of Iranian avian influenza isolates to infect human should be considered
Avian influenza (AI), Hemagglutinin (HA), H9N2 subtype, Phylogeny
167
172
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-222&slc_lang=en&sid=1
2011/12/4
1390/9/13
2004/10/15
1383/7/24
Vahid
Karimi
وحید
کریمی
00319475328460057491
00319475328460057491
No
Mohammad Hassan
Bozorgmehri Fard
محمد حسن
بزرگمهری فرد
00319475328460057492
00319475328460057492
No
Delavar
Shahbazzadeh
دلاور
شهباززاده
delavar@institute.pasteur.ac.ir
00319475328460057493
00319475328460057493
Yes
Majid
Esmaelizad
مجید
اسماعیل زاده
00319475328460057494
00319475328460057494
No
Seyed Ali
Pourbakhsh
سیدعلی
پوربخش
00319475328460057495
00319475328460057495
No
en
الگوی انتشار ژن مقاومت به متیسیلین (mecA) در استافیلوکوکوس آرئوسهای جدا شده از نمونههای کلینیکی
Distribution Patterns of Methicillin Resistance Genes (mecA) in Staphylococcus aureus Isolated from Clinical Specimens
There is a growing concern about the application of molecular methods in epidemiological studies of infectious diseases. The objective of this study was to determine the genetic stability of methicillin resistance genes in Staphylococcus aureus for the evaluation of resistance strain distribution. One hundred and fifteen S. aureus isolates from patients with staphylococcal infection were collected. The isolates were screened for methicillin resistance by minimum inhibitory concentration (MIC) examination. The stability of methcillin resistance genes was examined by physical curing and PCR screening methods. The results showed that methicillin resistance Staphylococcus aureus (MRSA) had risen up to 43% in Nemazi Hospital (Shiraz, Iran). Indeed, the incidence of MRSA in our hospital was 10% during the last four years. The ability to lose (curability) of methicillin resistance genes (mecA) was examined by physical curing method in 49 isolates with MIC ³ 16 μg ml-1. No sign of curability of mecA gene was observed where 500 colonies from each strain have been studied and exhibited by the same MIC values before and after curing test. Positive PCR results for isolates with MIC ³ 16 μg ml-1 before and after curing experiment have been achieved. These data confirm the results of curing method, indicating that stable genetic determinants confer methicillin resistance. These results support the hypothesis that resistance isolates may be selected due to clonal selection under antibiotic pressure used in clinics rather than transmission of mobile genetic determinant. The high prevalence of MRSA immerged in our Hospital could be originated due to antibiotic pressure and poor control measures
methicillin resistance genes (mecA), methicillin resistance Staphylococcus aureus (MRSA), minimum inhibitory concentration (MIC), Staphylococcus aureus
173
178
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-223&slc_lang=en&sid=1
2011/12/42011/12/4
1390/9/13
2004/10/152004/10/15
1383/7/24
Aziz
Japoni
عزیز
ژاپونی
japonia@hotmail.com
00319475328460057496
00319475328460057496
Yes
Abdolvahab
Alborzi
عبدالوهاب
البرزی
00319475328460057497
00319475328460057497
No
Fatemeh
Orafa
فاطمه
عرفا
00319475328460057498
00319475328460057498
No
Manoochehr
Rasouli
منوچهر
رسولی
00319475328460057499
00319475328460057499
No
Shohreh
Farshad
شهره
فرشاد
00319475328460057500
00319475328460057500
No
en
زمانبندی دادنیلاسیون و تحلیل mRNAهای مادری c-mos و cyclinA2 در طی مراحل اولیه جنین یک سلولی در موش
Time Course of Degradation and Deadenylation of Maternal c-mos and Cyclin A2 mRNA during Early Development of One-Cell Embryo in Mouse
Early in the development of many animals, before transcription begins, any change in the pattern of protein synthesis is attributed to a change in the translational activity or stability of mRNA in the egg and early embryo. As a result, translational control is critical for a variety of developmental decisions, including oocyte maturation and initiation of preimplantation development. In this study, using real-time RT-PCR method, we defined the time course of degradation and deadenylation of an oocyte specific gene (c-mos) more precisely and a gene that is re-synthesized after ZGA (cyclin A2). Our data indicate that oocyte-specific transcript, c-mos, degrades rapidly while cyclin A2 mRNA does not and the deadenylation of c-mos mRNA precedes the process of degradation. Our findings suggest that time-dependent elimination of different maternal mRNA is a way for regulation of translation in early development of mouse embryos
Maternal mRNA, Mouse embryo, mRNA degradation, c-mos, Cyclin A2
179
183
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-224&slc_lang=en&sid=1
2011/12/42011/12/42011/12/4
1390/9/13
2004/10/152004/10/152004/10/15
1383/7/24
Zohreh
Alizadeh
زهره
علیزاده
alizade_397@yahoo.com
00319475328460057501
00319475328460057501
Yes
Parichehr
Pasbakhsh
پریچهر
پاس بخش
00319475328460057502
00319475328460057502
No
Aligholi
Sobhani
علیقلی
صبحانی
00319475328460057503
00319475328460057503
No
Mohammad
Barbarestani
محمد
بربرستانی
00319475328460057504
00319475328460057504
No
Marefat
Ghafari
معرفت
غفاری
00319475328460057505
00319475328460057505
No
Faride
Etesam
فریده
اعتصام
00319475328460057506
00319475328460057506
No
en
تغییر میزان ترشح ایمونوگلوبولینها در کشت لنفوسیتهای B با استفاده از مهار تولید اینترلوکین 13 توسط اولیگونوکلئوتید آنتیسنس
Inhibition of IL-13 by Antisense Oligonucleotide Changes Immunoglobulin Isotype Profile in Cultured B-Lymphocytes
The link between IL-13 and bronchial hyper-responsiveness has brought this cytokine as a potential therapeutic target for asthma and allergic diseases. At the present study, we address the role of B cell derived IL-13 in the IgE and other immunoglobulin development. Antisense oligo for human IL-13 m-RNA was used to study IgE down regulation. Human B-lymphocytes were purified by positive selection using magnetic cell sorting and were cultured in the complete medium plus anti-CD40 monoclonal antibody and recombinant human IL-4. Immunoglobulin assay was performed by ELISA in the presence and absence of antisense oligonucleotide. We demonstrated that IL-13 antisense causes the decrease of IgE and increase of IgA significantly and no significant changes in IgM and IgG levels (p<0.01). We also demonstrated that both IL-13 inhibition and IL-4 removal cause the complete blocking of IgE and significant decrease of IgM and IgG levels. Our IL-13 antisense oligo can block B-cell IL-13 productions and consequently inhibits IgE production followed by IgA class switching in vitro. We suggest that in contrast to the IL-4, IL-13 is apparently more potent in the IgE switching and has no significant role in IgG and IgM levels
IL-13, IgE, Antisense oligo
185
191
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-225&slc_lang=en&sid=1
2011/12/42011/12/42011/12/42011/12/4
1390/9/13
2004/10/152004/10/152004/10/152004/10/15
1383/7/24
Tahereh
Mousavi
طاهره
موسوی
00319475328460057507
00319475328460057507
Yes
Bruce
Mazer
Bruce
Mazer
00319475328460057508
00319475328460057508
No
Majid
Tebianian
مجید
طبیانیان
00319475328460057509
00319475328460057509
No
en
اثر تجویز ساب کرونیک کاپتوپریل بر انقباض القاء شده توسط آگونیست آلفا یک آدرنوسپتور در آئورت ایزوله موش صحرایی
Effect of Subchronic Administration of Captopril on a1-Adrenoceptor Agonist-Induced Contraction of Isolated Aorta in Rat
Angiotensin II is a major endocrine hormone that affects directly both vascular smooth muscle and endothelial cells. Since vascular reactivity to angiotensin II changes in more physiological and pathophysiological conditions, the present study was performed to investigate the effect of intraperitoneal administration of angiotensin-converting enzyme inhibitor and captopril (30 and 50 mg kg-1, once daily for 8 weeks) on contractile response of rat aorta. After 8 weeks, the treated rats were anesthetized, their thoracic aortas were excised and placed in a Petri dish filled with Krebs solution for recording of contraction and relaxation response. The obtained results showed that captopril did not modify body weight gain and food or water intake but contractile response of aortic rings to phenylephrine in treated rats with 30 and 50 mg kg-1 captopril, in the presence of endothelium, decreases about 11-22% and 29-32% (P<0.05-P<0.01), respectively, when compared to the controls. Denuded aortic rings from 30 and 50 mg kg-1 captopril-treated rats showed 11-21% and 7-11% decrease in contractile response, respectively. There was a marked endothelium-dependent relaxation response to acetylcholine in 50 mg kg-1 captopril-treated rats compared to the controls (P<0.05). Endothelium-independent relaxation response to isosorbide dinitrate showed no significant difference in all groups. According to these results, it is suggested that captopril exerts its relaxant effect directly and/or indirectly through endothelium by production and releasing of endothelium-derived relaxing factors
Captopril, Aortic rings, Phenylephrine, Acetylcholine, Isosorbide dinitrate
193
198
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-226&slc_lang=en&sid=1
2011/12/42011/12/42011/12/42011/12/42011/12/4
1390/9/13
2004/10/152004/10/152004/10/152004/10/152004/10/15
1383/7/24
Tourandokht
Baluchnejad Mojarad
توراندخت
بلوچ نژاد مجرد
tmojarad@yahoo.com
00319475328460057510
00319475328460057510
Yes
Mehrdad
Roghani
مهرداد
روغنی
00319475328460057511
00319475328460057511
No
Homayoun
Homayounfar
همایون
همایونفر
00319475328460057512
00319475328460057512
No
en
تعیین مشخصات ملکولی و پراکندگی عوامل بیماریزا در آئروموناس هیدوربنلا جدا شده از نمونههای مدفوع در افراد اسهالی و افراد سالم بدون علامت در ایلام، ایران
Characterization and Distribution of Virulence Factors in Aeromonas hydrophila Strains Isolated from Fecal Samples of Diarrheal and Asymptomatic Healthy Persons, in Ilam, Iran
Aeromonas hydrophila secretes several extracellular proteins including enterotoxin, hemolysin and aerolysin that are associated with the bacterial virulence. Previous studies have shown that two hemolytic toxins, hemolysin A and aerolysin A contribute to the virulence of Aeromonas hydrophila. In the current study, a total of 50 strains of Aeromonas hydrophila, including 28 (56%) strains isolated from diarrheal cases and 22 (44%) strains isolated from healthy asymptomatic controls, were used. These strains were tested for cytochrome oxidase activity based on Kovac’s method and confirmed as A. hydrophila with API 20E multi-test systems. To determine hemolysin, cytotoxin and enterotoxin activities, horse blood agar, Vero cells and the suckling mouse model were used, respectively. The presence of two hemolytic toxin genes: hlyA and aerA was determined by PCR assay. About 89% of diarrheal strains tested were positive for hemolysins, 68% for cytotoxin and 89% for enterotoxin activity. These figures for healthy asymptomatic isolates were 72%, 23% and 14%, respectively. A significant association was found between cytotoxin and enterotoxin activity in diarrheal disease, whereas such association was not found in case of hemolysin. Almost 93% of diarrheal isolates and 73% of healthy person strains were PCR positive for hlyA gene. The corresponding figures for aerA gene were 86% and 45.5% respectively. The aerA+ hlyA+ genotype and in some cases aerA+ genotype could be considered as reasonable predictors of human diarrheal disease. However, the role of other unknown hemolytic and cytolytic factors cannot be discounted
Aeromonas, Suckling mouse, Aerolysin, Hemolysin, Cytotoxin
199
203
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-227&slc_lang=en&sid=1
2011/12/42011/12/42011/12/42011/12/42011/12/42011/12/4
1390/9/13
2004/10/152004/10/152004/10/152004/10/152004/10/152004/10/15
1383/7/24
Mohammad Me
Aslani
محمد مهدی
اصلانی
mmaslani@ institute.pasteur.ac.ir
00319475328460057521
00319475328460057521
Yes
Hassan
Seyyed Hamzeh
حسن
سید حمزه
00319475328460057522
00319475328460057522
No
en
تشخیص همزمان جنس هلیکوباکتر و گونه هلیکوباکتر پیلوری با استفاده از یک روش multiplex PCR
Simultaneous Detection of Helicobacter Genus and Helicobacter pylori Species using a Multiplex PCR Method
In order to improve simultaneous detection and identification of Helicobacter genus in general and Helicobacter pylori specifically and reduce the number of amplifications needed, we established a multiplex PCR. In this study, two pairs of primers: Hcom1 and Hcom2 specific for Helicobacter genus, Hicd1 and Hicd2 specific for Helicobacter pylori species were used. To determine the sensitivity of our multiplex PCR, the lower limits of DNA detection from pure culture were established using phenol-chloroform method. To evaluate the specificity of our protocol, we tested DNA extracted from various Gram-negative and -positive bacteria. A study was subsequently undertaken on stomach tissue samples from 18 patients to evaluate this protocol for detection of Helicobacter in tissue samples. In our optimized PCR, two fragments of 389- and 1200-bp were produced using Hcom1-Hcom2 and Hicd1-Hicd2 primers, respectively. Amplification of Helicobacter pylori genomic DNA was achieved at concentration as low as 0.03 pg equivalent to 150 bacteria. No DNA amplification of other Gram- positive and -negative bacteria was seen. Twelve specimens, positive in culture and rapid urease test for Helicobacter pylori were positive for DNA of this organism using this multiplex PCR. Our results demonstrate that this protocol represents a specific and sensitive assay for simultaneous detection of Helicobacter genus members, in general, and Helicobacter pylori species, specifically, in clinical samples yielding no false-positive
Helicobacter, Helicobacter pylori, Multiplex PCR
205
209
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-228&slc_lang=en&sid=1
2011/12/42011/12/42011/12/42011/12/42011/12/42011/12/42011/12/4
1390/9/13
2004/10/152004/10/152004/10/152004/10/152004/10/152004/10/152004/10/15
1383/7/24
Shohreh
Farshad
شهره
فرشاد
s_farshad@yahoo.com
00319475328460057513
00319475328460057513
Yes
Manoochehr
Rasouli
منوچهر
رسولی
00319475328460057514
00319475328460057514
No
Abdolvahab
Alborzi
عبدالوهاب
البرزی
00319475328460057515
00319475328460057515
No
en
تاثیر اشعه گاما بر تکثیر و تولید IL-5 توسط لنفوسیتهای خون محیطی
Effects of Gamma Irradiation on Proliferation and IL-5 Production of Peripheral Blood Lymphocytes
Gamma irradiation is routinely used for suppression of lymphocyte function in transfusion and transplantation procedures. In recent years, some investigators focused on the effects of ionizing radiation on special aspects of lymphocyte function and considered the possibility of its clinical application for treatment of some immunological disorders. In this study, we evaluated the effects of five different doses of &gamma-ray on proliferation and IL-5 production of peripheral blood lymphocytes. Lymphocytes were separated from blood and were treated with 5, 10, 20, 30 and 40 Gy irradiation (using a 137 Cs source) and then were cultured for 72 h in the presence of phytohemagglutinin (PHA). The proliferative response of samples was evaluated by MTT assay, and the supernatant of the cells was collected for IL-5 detection. The results showed that the ionizing radiation had a suppressive effect on lymphocyte proliferation. IL-5 production was affected in a dose-response manner, augmented in response to 5 and 10 Gy and reached to its peak value at 20 Gy. At 30 Gy, IL-5 production was diminished lower than peak value, but still remained higher than control baseline, and 40 Gy led to IL-5 values lower than baseline
Gamma irradiation, Lymphocyte proliferation, Th1/Th2 cytokines
211
214
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-229&slc_lang=en&sid=1
2011/12/42011/12/42011/12/42011/12/42011/12/42011/12/42011/12/42011/12/4
1390/9/13
2004/10/152004/10/152004/10/152004/10/152004/10/152004/10/152004/10/152015/11/23
1394/9/2
Maryam
Noorizadeh
مریم
نوری زاده
00319475328460057516
00319475328460057516
No
Jamshid
Hadjati
جمشید
حاجتی
hajatij@sina.tums.ac.ir
00319475328460057517
00319475328460057517
Yes
Alireza
Khabiri
علی رضا
خبیری
00319475328460057518
00319475328460057518
No
Mohammad
Vodjgani
محمد
وجگانی
00319475328460057519
00319475328460057519
No
Hajar
Khadem-Shariat
هاجر
خادم شریعت
00319475328460057520
00319475328460057520
No