en
jalali
1387
4
1
gregorian
2008
7
1
12
3
online
1
fulltext
en
Osteogenic Differentiation of Rat Mesenchymal Stem Cells from Adipose Tissue in Comparison with Bone Marrow Mesenchymal Stem Cells: Melatonin As a Differentiation Factor
Osteogenic Differentiation of Rat Mesenchymal Stem Cells from Adipose Tissue in Comparison with Bone Marrow Mesenchymal Stem Cells: Melatonin As a Differentiation Factor
Background: Adipose-derived stem cells (ADSC) could be an appealing alternative to bone marrow stem cells (BMSC) for engineering cell-based osteoinductive grafts. Meanwhile, prior studies have demonstrated that melatonin can stimulate osteogenic differentiation. Therefore, we assayed and compared the melatonin effect on osteogenic differentiation of BMSC with that of ADSC. Methods: Mesenchymal stem cells (MSC) were isolated from the bone marrow and fat of adult rats. Both cell types were cultured in osteogenic medium in the absence and presence of melatonin at physiological concentrations (20-200 pg/ml). After 4 weeks, the expression of osteocalcin gene was analyzed by reverse transcription-PCR, alkaline phosphatase (ALP) activity was assayed and alizarin red S and von Kossa staining were done. Cell viability and apoptosis were also assayed by 3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide, a tetrazole (MTT) and flow cytometry, respectively. Results: The osteoblastic differentiation of ADSC as demonstrated by ALP activity was less than that of BMSC. The amount of matrix mineralization has shown by alizarin red S and von Kossa staining also showed statistical differences between the two MSC. The incidence of apoptotic cells was higher among ADSC than BMSC. The flow cytometry proves that cell growth reduction is due to a decrease in the number of the cells entering the S phase of the cell cycle. MTT assay indicated that viable cells were fewer among ADSC than BMSC in control groups. Conclusion: The results of the study suggest that BMSC have greater osteogenic potential than ADSC and that melatonin promotes osteogenic differentiation to BMSC, but has a negative effect on ADSC osteogenic differentiation.
Mesenchymal stem cells (MSC), Bone marrow, Adipose tissue, Osteogenic, Melatonin
133
141
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-53&slc_lang=en&sid=1
2010/04/12
1389/1/23
2013/03/27
1392/1/7
Arash
Zaminy
آرش
زمینی
00319475328460056979
00319475328460056979
No
Iraj
Ragerdi Kashani
ایرج
راگردی کاشانی
ragerdi@sina.tums.ac.ir
00319475328460056980
00319475328460056980
Yes
Mohammad
Barbarestani
محمد
بربرستانی
00319475328460056981
00319475328460056981
No
Azim
Hedayatpour
عظیم
هدایت پور
00319475328460056982
00319475328460056982
No
Reza
Mahmoudi
رضا
محمودی
00319475328460056983
00319475328460056983
No
Ahmadreza
Farzaneh Nejad
احمدرضا
فرزانه نژاد
00319475328460056984
00319475328460056984
No
en
Beta Cell Protective Effects of Sodium Tungstate in Streptozotocin-Induced Diabetic Rats: Glycemic Control, Blockage of Oxidative Stress and Beta Cell Histochemistry
Beta Cell Protective Effects of Sodium Tungstate in Streptozotocin-Induced Diabetic Rats: Glycemic Control, Blockage of Oxidative Stress and Beta Cell Histochemistry
Background: Diabetes is a major public health problem. The development of new therapies that are able to improve glycemia management and even to cure diabetes is of great interest. In this study, protective effects of sodium tungstate against STZ-induced beta-cell damages were investigated. Methods: Sixty rats were divided into six groups: control, diabetic, sodium tungstate treated diabetic rats from one week before STZ injection (TDB), food-restricted diabetic (FRD), tungstate treated control, sodium tungstate treated diabetic rats from one week after STZ administration (TDA). We evaluated serum insulin, glucose and glucose tolerance liver glycogen content, glucokinase (GK) activity blood and pancreas antioxidant power, lipid peroxidation and fuchsin-aldehyde histochemical staining of beta-cells. Results: Blood glucose levels of TDB group were lower than other diabetic groups (P<0.01). Blood insulin levels of all diabetic groups were lower than controls (P<0.01). Glucose intolerance improved in TDB animals. Blood and pancreas antioxidant power, liver glycogen contents and GK activities and granulated beta cells increased in TDB rats in comparison with other diabetic groups (P<0.01). Likewise, lipid peroxidation decreased significantly in TDB rats (P<0.01). Conclusions: Results suggested that sodium tungstate if administrated before STZ injection improves glycemic state by a direct effect on pancreatic beta-cells and preserves them by reducing the activity of these cells at the time of STZ injection, reducing STZ-induced oxidative stress, reducing insulin secretion, or all of the above mentioned.
Diabetes mellitus, Sodium tungstate, Beta cells
143
152
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-48&slc_lang=en&sid=1
2010/04/122010/04/7
1389/1/18
2013/03/272013/03/27
1392/1/7
Zahra
Heidari
زهرا
حیدری
histology_iri@yahoo.com
00319475328460056985
00319475328460056985
Yes
Mehdi
Harati
مهدی
هراتی
00319475328460056986
00319475328460056986
No
Hamid Reza
Mahmoudzadeh-Sagheb
حمیدرضا
محمودزاده ثاقب
00319475328460056987
00319475328460056987
No
Bita
Moudi
بیتا
مودی
00319475328460056988
00319475328460056988
No
en
CD147 (Extracellular Matrix Metalloproteinase Inducer-EMMPRIN) Expression by Human Articular Chondrocytes
CD147 (Extracellular Matrix Metalloproteinase Inducer-EMMPRIN) Expression by Human Articular Chondrocytes
Background: Integrins are a family of transmembrane proteins that allow communication between the extracellular matrix and the interior of cells. Chondrocytes, cells of articular cartilage, express integrins and these molecules appear to have a variety of roles including mechanotransduction. Integrins are known to associate with a number of accessory molecules such as CD147 that may act to regulate their activity. The purpose of this study was to investigate the expression of CD147 in normal and osteoarthritis human articular cartilage and identify potential roles in mechanical signalling. Methods: Expression of CD147 in normal and osteoarthritis human articular cartilage was examined by the immunostaining and Western-blotting techniques. Potential roles in mechanotransduction were studied by assessing effects of function blocking antibodies on the electrophysiological response to mechanical stimulation. Results: CD147 was extensively expressed by chondrocytes in normal and osteoarthritic cartilage and shown by Western-blotting to have a molecular weight in the region of 35-50 kDa. Function blocking antibodies had no effect on the membrane depolarisation response of chondrocytes from osteoarthritic cartilage to mechanical stimulation. Conclusion: Human articular chondrocytes show extensive expression of CD147 in normal and osteoarthritic cartilage. Roles for this molecule in regulation of chondrocyte function remain to be defined.
Chondrocyte, Articular cartilage, Osteoarthritis, Extracellular matrix metalloproteinase inducer (EMMPRIN)/CD147, Integrin
153
158
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-50&slc_lang=en&sid=1
2010/04/122010/04/72010/04/7
1389/1/18
2013/03/272013/03/272013/03/27
1392/1/7
Mahmoud
Orazizadeh
محمود
اوراضی زاده
M_orazizadeh@yahoo.com
00319475328460056989
00319475328460056989
Yes
Donald M.
Salter
Donald M.
Salter
00319475328460056990
00319475328460056990
No
en
Comparison of Distribution of Virulence Determinants in Clinical and Environmental Isolates of Vibrio cholera
Comparison of Distribution of Virulence Determinants in Clinical and Environmental Isolates of Vibrio cholera
Background: The virulence of a pathogenic Vibrio cholerae is dependent on a discrete set of genetic determinants. In this study, we determined the distribution of virulence determinants among the clinical and environmental isolates of V. cholerae. Methods: The antibiotic resistance profiles of the isolates were determined using standard disk diffusion assay. PCR assay was performed to analyze the presence of toxin genes of ctx, zot and ace. The composition of cholera toxin encoding element (CTX) region flanking of the V. cholerae isolates was also analyzed. Results: All of the clinical isolates (100%) showed a complete set of virulence genes and also the attachment site of the filamentous bacteriophage CTXf. None of the environmental isolates contained the virulence genes and the attachment site of the CTXf. Analysis of the flanking regions including the toxin-linked cryptic element and repeat in toxin genes revealed their integrity in the clinical isolates while in the environmental isolates they were absent or contained incomplete sequences. Comparison of the antibiotic resistance assay of the environmental and clinical isolates showed a significant difference in the resistance profiles of the isolates obtained from the two sites. High rates of resistance to co-trimoxosol, streptomycin and chloramphenicol were found with clinical isolates. Conclusion: The absence of all virulence determinants in the environmental strains may suggest that certain ecological features must be present for V. cholerae to acquire a complete set of virulence determinants and to turn them into pathogenic strains.
Vibrio cholerae, Virulence determinants, Environment
159
165
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-52&slc_lang=en&sid=1
2010/04/122010/04/72010/04/72010/04/12
1389/1/23
2013/03/272013/03/272013/03/272013/03/27
1392/1/7
Bita
Bakhshi
بیتا
بخشی
00319475328460056991
00319475328460056991
No
Mohammad Reza
Pourshafie
محمدرضا
پورشفیع
pour@pasteur.ac.ir
00319475328460056992
00319475328460056992
Yes
Farahtaj
Navabakbar
فرح تاج
نواب اکبر
00319475328460056993
00319475328460056993
No
Akbar
Tavakoli
اکبر
توکلی
00319475328460056994
00319475328460056994
No
Fereshteh
Shahcheraghi
فرشته
شاهچراغی
00319475328460056995
00319475328460056995
No
Mansoor
Salehi
منصور
صالحی
00319475328460056996
00319475328460056996
No
Ziba
Faradjzadegan
زیبا
فرج زادگان
00319475328460056997
00319475328460056997
No
Seyed Mohsen
Zahraei
سید محسن
زهرایی
00319475328460056998
00319475328460056998
No
en
Pectic Acid Effects on Prolactin Secretion in GH3/B6 Rat Pituitary Cell Line
Pectic Acid Effects on Prolactin Secretion in GH3/B6 Rat Pituitary Cell Line
Background: Pectic acid extracted from plants increases the secretion of prolactin (PRL) when injected intravenously into ewes or fed to rats. Fragments of ewe hypophysis and lactating rabbit mammary gland incubated in vitro in the presence of pectic acid secreted more PRL and caseins compared to the controls. However, it is not known whether pectic acid directly stimulates PRL secretion in pituitary or interference of factors from hypophysis is required for this process. Methods: GH3/B6 cells, a clonal strain of rat pituitary, were cultured and incubated with pectic acid (2.5-100 mg/mL). The integrity of cells was examined under pectic acid treatment microscopically. Controls or pectic acid treated cells were assayed for their ability to produce PRL. The PRL was assayed by Western-blotting and Radioimmunoassay. Results: pectic acid did not have any significant effect on the viability of cells. After being incubated with pectic acid, the cells started to become circular and protuberant shape. The maximum stimulation and PRL secretion occurred at 100 mg/mL concentration within 30 min of incubation with pectic acid. Conclusion: pectic acid could stimulate the release of PRL in GH3/B6 cells in the short-term incubation. This result suggested that pectic acid is a non-toxic agent that could directly stimulate PRL secretion in pituitary cells without any interference of hypophysis.
Pectic acid, Prolactin, Pituitary, GH3/B6 cells
167
172
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-46&slc_lang=en&sid=1
2010/04/122010/04/72010/04/72010/04/122010/04/7
1389/1/18
2013/03/272013/03/272013/03/272013/03/272013/03/27
1392/1/7
Delaram
Eslimi
دلارام
اسلیمی
00319475328460056999
00319475328460056999
No
Houri
Sepehri
حوریه
سپهری
00319475328460057000
00319475328460057000
No
Yasaman
Rassouli
یاسمن
رسولی
00319475328460057001
00319475328460057001
No
Samideh
Khoei
سمیده
خویی
00319475328460057002
00319475328460057002
No
Bahram
Goliaei
بهرام
گلیایی
goliaei@ibb.ut.ac.ir
00319475328460057003
00319475328460057003
Yes
en
A Study of Acute and Chronic Anti-Nociceptive and Anti-Inflammatory Effects of Thiamine in Mice
A Study of Acute and Chronic Anti-Nociceptive and Anti-Inflammatory Effects of Thiamine in Mice
Background: Thiamine (VitB1) is a vitamin with various important physiological functions and postulated therapeutic effects. Its use as an analgesic in neuropathic pain has been undergoing in clinical settings. However, there has been little experimental investigation on this effect. In this study, anti-nociceptive and anti-inflammatory effects of thiamine were investigated in mice. Methods: Three doses of thiamine (50, 100 and 125 mg/kg) were used by intraperitoneal injection in this study. Acute and chronic anti-nociceptive effects were examined using hot plate test alone and after sciatic nerve ligation, respectively. Imipramine (40 mg/kg) was used as positive control. Anti-inflammatory effects of thiamine on acute and chronic inflammation were assessed using xylene-induced edema in ears and granuloma caused by compressed cotton implantation, respectively. Sodium diclofenac (15 mg/kg) was used as positive control. Open field test was performed to differentiate the mice responses in the acute anti-nociceptive tests. Results: All three doses of thiamine showed significant analgesic effects in non-ligated mice and also in neuropathic pain in ligated animals. Increasing the dose of thiamine correlated with a more pronounced and sustained effect. Acute anti-inflammatory investigation showed that thiamine injected 30 or 60 minutes before xylene application reduced the weight of edematic ears. However, the effect of thiamine was less pronounced than diclofenac. Furthermore, when injected once daily for 7 days, all doses of thiamine significantly reduced the weight of the cotton disks, showing suppression of granuloma formation. Conclusion: Taken together, it has been shown that thiamine possesses remarkable analgesic activities and also has significant anti-inflammatory effects, confirming its clinical use in controlling pain and less in inflammation.
Thiamine, Anti-nociceptive, Anti-inflammation
173
178
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-49&slc_lang=en&sid=1
2010/04/122010/04/72010/04/72010/04/122010/04/72010/04/7
1389/1/18
2013/03/272013/03/272013/03/272013/03/272013/03/272015/09/9
1394/6/18
Seyed Adel
Moallem
سید عادل
معلم
moallem@mums.ac.ir
00319475328460057004
00319475328460057004
Yes
Hossein
Hosseinzadeh
حسین
حسین زاده
00319475328460057005
00319475328460057005
No
Sepideh
Farahi
سپیده
فرهی
00319475328460057006
00319475328460057006
No
en
L-Arginine Supplementation Influenced Nitrite but Not Nitrate and Total Nitrite in Rabbit Model of Hypercholesterolemia
L-Arginine Supplementation Influenced Nitrite but Not Nitrate and Total Nitrite in Rabbit Model of Hypercholesterolemia
Background: The assessment of altered nitric oxide (NO) availability is of potentially important diagnostic and prognostic significance. The present study is aimed to investigate the effect of L-arginine (as a natural NO donor) supplementation on NO metabolite in a rabbit model of hypercholesterolemia to find a reliable marker for endothelial NO production. Methods: White male rabbits (n = 30) randomly assigned to 2 groups. Rabbits were fed 1% high-cholesterol diet (HC group, n = 15), or HC diet with oral L-arginine (3% in drinking water) (HC + L-arginine group, n = 15) for 4 weeks. The serum levels of lipids, L-arginine, total NO metabolites (NOx), nitrite and nitrate were measured before and after the study. Results: In this study, L-arginine supplementation led to a significant increased plasma level of L-arginine. The serum level of nitrite was significantly higher in L-arginine treated group while serum level of nitrate and NOx was significantly lower than HC group. Conclusion: As the result of our study showed, nitrite is a useful marker of endogenous endothelial NO production and although frequently used, neither nitrate nor NOx are reliable markers of acute changes in endothelial NO synthase activity.
Nitric oxide (NO), L-arginine, Nitrite, Nitrate, Total nitrite
179
184
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-47&slc_lang=en&sid=1
2010/04/122010/04/72010/04/72010/04/122010/04/72010/04/72010/04/7
1389/1/18
2013/03/272013/03/272013/03/272013/03/272013/03/272015/09/92013/03/27
1392/1/7
Shaghayegh
Haghjooy Javanmard
شقایق
حق جوی جوانمرد
sh_haghjoo@med.mui.ac.ir
00319475328460057007
00319475328460057007
Yes
Mehdi
Nematbakhsh
مهدی
نعمت بخش
00319475328460057008
00319475328460057008
No
Alireza
Monajemi
علیرضا
منجمی
00319475328460057009
00319475328460057009
No
en
Anti-Microbial Resistance of Enterococci Isolated from Urinary Tract Infections in Iran
Anti-Microbial Resistance of Enterococci Isolated from Urinary Tract Infections in Iran
Background: During the last decade, enterococci have become important nosocomial pathogens, representing the second leading cause of urinary tract infections. This increasing prevalence has been paralleled by the occurrence of multi-drug resistant (MDR) and high-level gentamicin resistant (HLGR) strains. Methods: From September 2005 to 2006, a total of 638 enterococcal isolates were collected from urine samples among 9 medical centers in Tehran (Iran). Confirmation of species and detection of gentamicin resistance genes were done by PCR method. Anti-microbial susceptibility test was determined with disk diffusion and minimal inhibitory concentration of gentamicin among HLGR isolates assayed by microdilution methods. Results: The isolates were found to consist of Enterococcus faecalis (77.8%) and Enterococcus faecium (22.2%). The results obtained from PCR showed a high rate of agreement with phenotypic assays for both species. MDR to most prevalent anti-microbials was present in 29% and 72% of the E. faecalis and E. faecium isolates, respectively. HLGR phenotype was detected in 64% of E. faecalis and 92% of E. faecium isolates. The aac(6')-Ie-aph(2")-Ia gene were identified in 83% of E. faecalis and 100% of E. faecium HLGR isolates. E. faecalis and E. faecium isolates differed in their susceptibilities to different antibiotics. Conclusion: Emergence of multi-resistant enterococci and high level resistance to gentamicin shown by enterococcal strains is of concern because of the decrease in the therapeutic options for treatment of infections caused by enterococci.
Anti-microbial resistance, Enterococci, Urinary tract infection (UTI)
185
190
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-54&slc_lang=en&sid=1
2010/04/122010/04/72010/04/72010/04/122010/04/72010/04/72010/04/72010/04/12
1389/1/23
2013/03/272013/03/272013/03/272013/03/272013/03/272015/09/92013/03/272013/03/27
1392/1/7
Mahnaz
Saifi
مهناز
سیفی
00319475328460057010
00319475328460057010
No
Mohammad Reza
Pourshafie
محمدرضا
پورشفیع
00319475328460057011
00319475328460057011
No
Mohammad Reza
Eshraghian
محمدرضا
اشراقیان
00319475328460057012
00319475328460057012
No
Mohammad Mehdi
Soltan Dallal
محمد مهدی
سلطان دلال
soltanda@tums.ac.ir
00319475328460057013
00319475328460057013
Yes
en
Evaluation of Bronchodilator and Anti-Anaphylactic Activity of Myrica sapida
Evaluation of Bronchodilator and Anti-Anaphylactic Activity of Myrica sapida
Background: Asthma is a chronic inflammatory disorder of the airways. The available treatment options have major limitations owing to low efficacy, associated adverse events and compliance issues. Therefore, the health burden of bronchial asthma is increasing globally at an alarming rate, providing a strong impetus for the development of new therapeutics. Myrica sapida is known traditionally in Ayurveda to possess anti-asthmatic activity. Hence, the present investigation was undertaken to evaluate the bronchodilator and anti-anaphylactic activity of the stem bark of Myrica sapida. Methods: Experimental models studied were acetylcholine induced bronchospasm in guinea pigs, egg albumin induced anaphylaxis in guinea pigs, in vitro studies on tracheal strip of egg albumin sensitized guinea pigs. Results: Treatment with ethanolic extract of M. sapida, 75 mg/kg, orally resulted in significant protection against acetylcholine aerosol induced bronchospasm and allergen induced anaphylaxis in guinea pigs. Ethanolic extract of M. sapida (75 mg/kg, p.o.) prevented the potentiation of responses and also produced a decrease in pD2 value of histamine and acetylcholine in guinea pig tracheal strip. Conclusion: These results suggest that M. sapida possesses bronchodilator activity, has potent inhibitory effect on immediate hyper-sensitivity reactions and decreases bronchial hyper-responsiveness.
Anti-asthmatic activity, Myrica sapida, Bronchial hyper-responsiveness, Potentiation
191
196
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-51&slc_lang=en&sid=1
2010/04/122010/04/72010/04/72010/04/122010/04/72010/04/72010/04/72010/04/122010/04/12
1389/1/23
2013/03/272013/03/272013/03/272013/03/272013/03/272015/09/92013/03/272013/03/272013/03/27
1392/1/7
Kalpana G.
Patel
Kirti V.
Patel
kalpana_jpatel@yahoo.com
00319475328460057014
00319475328460057014
Yes
Payal N.
Bhalodia
Ankita D.
Patel
00319475328460057015
00319475328460057015
No
Ankita D.
Patel
Payal N.
Bhalodia
00319475328460057016
00319475328460057016
No
Kirti V.
Patel
Kalpana G.
Patel
00319475328460057017
00319475328460057017
No
Tejal R.
Gandhi
Tejal R.
Gandhi
00319475328460057018
00319475328460057018
No