@article{ author = {Khosravi, Azar D. and Stanford, John L}, title = {Differentiation of M. scrofulaceum from Related Mycobacterial Species by Double-Diffusion Technique}, abstract ={Isolation and differentiation of various species of mycobacteria are predominantly based on conventional, cultural and biochemical tests. Among the non-tuberculous mycobacteria, these tests are unable to differentiate M. scrofulaceum from M. avium-intracellulare (MAI) because of their identical biochemical characteristics. For this reason, one hundred mycobacterial strains suspected to be M. scrofulaceum, which had identical preliminary cultural and biochemical tests, were investigated by immunodiffusion technique. The standard sonicated antigens of M. scrofulaceum (22, 159, 1281) were used to sensitize rabbits and to produce specific antisera. The method was performed in plastic petri dishes containing agar and sodium azide. Antiserum was placed in the central well of the agar plate and the homologous ultrasonicates were in the peripheral wells. The results were observed one week later and the mycobacterial strains were divided according to the number of produced precipitation lines. Four different groups were identified on the basis of identical reaction with three antisera (three groups were M. scrofulaceum). Group 1 produced identical precipitation lines with antisera 22 and 1281, group 2 with antisera 22, 159 and 1281 and group 3 with antiserum 1281. The present study showed that the technique was able to differentiate M. scrofulaceum from MAI and was also able to identify the bacterium as distinct species. Moreover, by using different specific antisera, it is possible to determine different serotypes of M. scrofulaceum.}, Keywords = {Double diffusion, lymphadenitis, M. scrofulaceum, M avium complex}, volume = {4}, Number = {4}, pages = {113-116}, publisher = {Pasteur Institute of Iran}, title_fa = {Differentiation of M. scrofulaceum from Related Mycobacterial Species by Double-Diffusion Technique}, abstract_fa ={}, keywords_fa = {}, url = {http://ibj.pasteur.ac.ir/article-1-590-en.html}, eprint = {http://ibj.pasteur.ac.ir/article-1-590-en.pdf}, journal = {Iranian Biomedical Journal}, issn = {1028-852X}, eissn = {2008-823X}, year = {2000} } @article{ author = {MohitiArdakani, javad and Walker, John and Qujeq, Durdi}, title = {Serum Factors Induced the Nuclear Location of Annexin V in the Human Osteosarcoma Cell Line (MG-63)}, abstract ={Calcium-binding proteins play essential roles in the cell. One important class of calcium-binding proteins is the annexin family. This is a family of 13 proteins, which binds to phospholipids in a calcium-dependent manner. Osteosarcoma cell line (MG-63) is a transformed cell that has many characteristics of the differentiated cell, such as a considerable serum dependency in its growth rate. Using specific antibodies against each annexin and immunoflurescence microscopy, the location and relocation of the annexin V was determined by some serum factors. Serum starvation of MG-63 cells increases their doubling time from 24 hours to 4 days. Cells grown in serum contain high levels of annexin V in the cell nucleus whereas in the absence of serum results in loss of nuclear annexin V in about 75% of the cells. Refeeding cells with medium containing 10% serum restore annexin V to the nuclei within 5 hours. Charcoal-treated serum cannot allow annexin V to return to the nucleus. Inhibition of protein synthesis with cycloheximide does not prevent the serum-induced return of annexin V to the nuclei. However, treatment of cells with genistein at a concentration specific for inhibition of tyrosine kinases (200 M) inhibits the relocation of annexin V from cytoplasm to the nucleus. Thus, the cellular location of the annexin V depends on the growth state of the cells. It can be altered by the movement of this protein between the cytosol and the nucleus.}, Keywords = {Annexin V , Calcium , Osteosarcoma cells}, volume = {4}, Number = {4}, pages = {117-122}, publisher = {Pasteur Institute of Iran}, title_fa = {Serum Factors Induced the Nuclear Location of Annexin V in the Human Osteosarcoma Cell Line (mg-63)}, abstract_fa ={}, keywords_fa = {}, url = {http://ibj.pasteur.ac.ir/article-1-591-en.html}, eprint = {http://ibj.pasteur.ac.ir/article-1-591-en.pdf}, journal = {Iranian Biomedical Journal}, issn = {1028-852X}, eissn = {2008-823X}, year = {2000} } @article{ author = {Jazayeri, Hadi and Motazedian, Mohammad Hossein and Emami, Masou}, title = {Genomic Linkage Analysis of Iranian Clinical Isolates of Dermatophytes Fungi Using the RAPD-PCR}, abstract ={Dermatophytes are a group of keratinophilic fungi capable of invading keratinized tissues (skin, hair and nails). They cause dermatophytosis (commonly known as tinea or Ring worm) in human and animals. In this report, DNA similarities and genomic linkage of 40 dermatophytes strains was obtained from different universities, were studied by random amplified polymorphic DNA (RAPD–PCR) using 11 random primers. The similarity of Microsporum genus with two other genera was 13%, and the similarity of Trichophyton with Epidermophyton was 20.8%. These results provide the basis for the rapid identification of dermatophytes at the genetic level, in additions to the existing laboratory methods.}, Keywords = {Genomic linkage, Dermatophytes, RAPD-PCR}, volume = {4}, Number = {4}, pages = {123-128}, publisher = {Pasteur Institute of Iran}, title_fa = {Genomic Linkage Analysis of Iranian Clinical Isolates of Dermatophytes Fungi Using the RAPD-PCR}, abstract_fa ={}, keywords_fa = {}, url = {http://ibj.pasteur.ac.ir/article-1-592-en.html}, eprint = {http://ibj.pasteur.ac.ir/article-1-592-en.pdf}, journal = {Iranian Biomedical Journal}, issn = {1028-852X}, eissn = {2008-823X}, year = {2000} } @article{ author = {Zavareh, Alirez}, title = {Immunogenicity of BHK-Rabies Vaccine in Cattle}, abstract ={In this study, the immunogenicity of a rabies vaccine that was used in one of the Isfahan cattle farms is reported. This vaccine was produced by replication of the Pasteur virus (PV) strain on BHK-21 monolayer cell culture. The virus suspension was inactivated by ß-propiolactone treatment and the harvested pool showed sterility and safety. Thus, the obtained vaccine was tested on mice and a satisfactory immune response was observed. Thereafter, the vaccine was tested on 34 cattle divided into 2 groups. Each group was received a different dose of the vaccine intramuscularly. Sera were taken 2 months after rabies vaccination and were analyzed by the rapid fluorescence focus inhibition test (RFFIT) to evaluate the titer of the rabies-neutralizing antibody. Both groups under study showed a sufficient seroconversion.}, Keywords = {Rabies, Anti-rabies vaccine, BHK-21 cell, Cattle immunization}, volume = {4}, Number = {4}, pages = {129-131}, publisher = {Pasteur Institute of Iran}, title_fa = {Immunogenicity ofBHK-Rabies Vaccine in Cattlei}, abstract_fa ={}, keywords_fa = {}, url = {http://ibj.pasteur.ac.ir/article-1-593-en.html}, eprint = {http://ibj.pasteur.ac.ir/article-1-593-en.pdf}, journal = {Iranian Biomedical Journal}, issn = {1028-852X}, eissn = {2008-823X}, year = {2000} } @article{ author = {ZareiMahmoudabadi, Ali and BernardDrucker, david and Mandall, Nicky and O'Brien, Kevin and Theaker, Elizabeth}, title = {Isolation and Identification of Candida Species from the Oral Cavity Using CHROMagar Candida}, abstract ={CHROMagar Candida is a new chromogenic culture medium that is used for the isolation and direct identification of some of the most clinically important yeast pathogens on the basis of colony colour. The aim of the study was to isolate and identify Candida species from the oral cavity with CHROMagar Candida. Samples (158) from 68 orthodontic patients wearing upper removable orthodontic appliances and 51 oral medicine patients were cultured on CHROMagar Candida at 37C for 48 h. Isolates with green colonies in colour were all confirmed as C. albicans (53) by germ tube test and others were confirmed as C. parapsilosis (10) and C. glabrata (3) by Rapid ID 32 C kits. There were 11 cases of mixed yeast cultures in this study that were easily recognised using the medium. In conclusion, C. albicans is the most frequently isolated yeast from the oral cavity of both orthodontic and oral medicine patients. Moreover CHROMagar Candida is a useful culture medium for the isolation and direct identification of Candida species, especially mixed cultures.}, Keywords = {CHROMagar Candida, Candida albicans, Candida parapsilosis}, volume = {4}, Number = {2}, pages = {57-61}, publisher = {Pasteur Institute of Iran}, title_fa = {}, abstract_fa ={}, keywords_fa = {}, url = {http://ibj.pasteur.ac.ir/article-1-822-en.html}, eprint = {http://ibj.pasteur.ac.ir/article-1-822-en.pdf}, journal = {Iranian Biomedical Journal}, issn = {1028-852X}, eissn = {2008-823X}, year = {2000} } @article{ author = {Mosayyebi, Ghasem and Shokri, Fazel}, title = {Comparative Measurement of Rheumatoid Factor in Serum and Synovial Fluid of Rheumatoid Arthritis Patients by ELISA and Latex-Agglutination Test}, abstract ={Rheumatoid factors (RF) are autoantibodies with specificity for the Fc portion of human IgG. Production of RF is a characteristic feature of rheumatoid arthritis (RA) and is detectable in high titer in about 90% of these patients. In this study, we measured total IgM, IgA, IgMRF and IgARF in serum and synovial fluid of 45 RA patients by ELISA and the results were compared with those obtained by latex agglutination test (LAT). The results show that from 45 RA patients, 93% (43:45) and 78% (35:45) were seropositive in ELISA and LAT, respectively. The sensitivity limit of LAT was approximately 10 µg/ml, whereas less than 10 ng/ml of RF was detectable by ELISA. There was a highly significant correlation between the concentration of IgMRF and latex agglutination titer in both serum (r = 0.78, p<0.0005) and synovial fluid (r = 0.66, p<0.05). Less significant correlations were observed for IgARF in serum (r = 0.65, p<0.05). While the concentration of total IgM and IgA were significantly higher in serum compared to synovial fluid, this however, was not reflected in the IgMRF or IgARF titer. In summary, our findings suggest that employment of isotype specific ELISA is inevitable and necessary for the quantitative measurement of RF isotypes other than IgM, which may be of clinical importance for diagnosis of disease severity and extra-articular complications of RA.}, Keywords = {Rheumatoid factor, Rheumatoid arthritis, ELISA, Latex-agglutination test}, volume = {4}, Number = {2}, pages = {63-67}, publisher = {Pasteur Institute of Iran}, title_fa = {}, abstract_fa ={}, keywords_fa = {}, url = {http://ibj.pasteur.ac.ir/article-1-823-en.html}, eprint = {http://ibj.pasteur.ac.ir/article-1-823-en.pdf}, journal = {Iranian Biomedical Journal}, issn = {1028-852X}, eissn = {2008-823X}, year = {2000} } @article{ author = {Qujeq, Durdi and Ataei, Gholamrez}, title = {Effects of Dietary Chitosan on Serum Lipid and Lipoprotein Concentration in Rats}, abstract ={Because the ingestion of some types of dietary fibers has been shown to influence on the lipid and lipoprotein levels, it is possible that chitosan influences on lipid metabolism. In the present study, the effects of chitosan on the serum, liver lipid and lipoprotein concentrations in rat were investigated. Serum lipid level in the treatment groups were decreased compared to that of the control, cholesterol level [128.65 +/- 2.58 (mean +/- SD, n = 72) vs. 173.67 +/- 3.62, p<0.05] mg/dl, triglyceride level [62.83 +/- 2.73 (mean +/- SD) vs. 93.62 +/- 2.64, p<0.05] mg/dl, and low density lipoprotein-cholesterol level [108.35 +/- 2.41(mean +/- SD) vs. 156.49 +/- 2.37, p<0.05] mg/dl. In the chitosan treatment group, high density lipoprotein-cholesterol level was increased as compared to the control [187.39 +/- 2.74 (mean +/- SD) vs. 163.54 +/- 2.83, p<0.05] mg/dl. This work showed that the addition of chitosan to the diet of the rats significantly lowered the liver lipid in the treatment groups compared to that of the control, cholesterol level [31.53 +/- 1.26(mean +/- SD) vs. 64.42 +/- 2.38, p<0.05] mg/g, and triglyceride level [38.46 +/- 2.64 (mean +/- SD) vs. 53.24 +/- 2.45, p<0.05] mg/g. When chitosan fed at the 5% level, concentration of the serum cholesterol was reduced by 25.92% and triglyceride by 32.89%. The data presented here indicated possible usefulness of chitosan for the treatment of hyperlipidemia.}, Keywords = {Chitosan, Cholesterol, Lipoprotein}, volume = {4}, Number = {2}, pages = {69-73}, publisher = {Pasteur Institute of Iran}, title_fa = {}, abstract_fa ={}, keywords_fa = {}, url = {http://ibj.pasteur.ac.ir/article-1-824-en.html}, eprint = {http://ibj.pasteur.ac.ir/article-1-824-en.pdf}, journal = {Iranian Biomedical Journal}, issn = {1028-852X}, eissn = {2008-823X}, year = {2000} } @article{ author = {Rezaee, Abbas and BehzadianNejad, Qorban and Kebriaeezadeh, Abbas}, title = {Simulataneous Analysis of Trimethoprim and Sulphamethoxazole Drug Combinations in Dosage Forms by High Performance Liquid Chromatography}, abstract ={A sensitive high performance liquid chromathography (HPLC) analytical procedure was developed for the quantitive determination of trimethoprim (TM* and sulphamethoxazole (SM) in commercial dosage forms. C18 analytical column (stainless steel, 25 cm * 4.6 mm i.d.) was packed with 5-*m particles of the reversed phase material and used for assays. Mobile phase containing 0.025 M sodium phosphate as aqueous phase and acetonitrile with 0.4% triethylamine as organic phase. The drugs were quantified at flow-rate of 1.2 ml/min, with ultraviolet detection at 260 nm. The minimum detectable quantities in assays were 100 ng/ml for SM and 75 ng/ml for TM. The method is well suited to routine application and adequate sensitivity with precision.}, Keywords = {Trimethoprim, Sulphamethoxazole, Co-trimoxazole, HPLC}, volume = {4}, Number = {2}, pages = {75-78}, publisher = {Pasteur Institute of Iran}, title_fa = {}, abstract_fa ={}, keywords_fa = {}, url = {http://ibj.pasteur.ac.ir/article-1-825-en.html}, eprint = {http://ibj.pasteur.ac.ir/article-1-825-en.pdf}, journal = {Iranian Biomedical Journal}, issn = {1028-852X}, eissn = {2008-823X}, year = {2000} } @article{ author = {Heidaran, mohammad A.}, title = {Tissue Engineering: A Biological Solution for Tissue Damage, Loss or End Stage Organ Failure}, abstract ={In recent years the science of tissue engineering has emerged as a powerful tool for the development of a novel set of tissue replacement parts and technologies. Recent advances in the fields of biomaterials, stem cell technologies, growth factor field and biomimetics have created a unique set of opportunities for investigators to fabricate lab-grown tissues from combination of extracellular matrices (scaffolds), cells, and bioactive molecules. Despite these breakthrough advances, the major challenges facing this new emerging field of bioengineering remain unresolved as lab-grown tissues still exhibit a general lack of functional and biomechanical stability needed for transplantation. A successful strategy to develop true human replacement parts requires a multidisciplinary approach that converges recent advances in tissue, matrix, growth factor and developmental biology with recent technological breakthroughs in tissueinformatics, bioinformatics, highthrouput combinatorial chemistry and stem cell technologies.}, Keywords = {Extracellular matrices, Stem cells, Biomaterial, Tissue engineering, Growth factors}, volume = {4}, Number = {1}, pages = {1-5}, publisher = {Pasteur Institute of Iran}, title_fa = {}, abstract_fa ={}, keywords_fa = {}, url = {http://ibj.pasteur.ac.ir/article-1-826-en.html}, eprint = {http://ibj.pasteur.ac.ir/article-1-826-en.pdf}, journal = {Iranian Biomedical Journal}, issn = {1028-852X}, eissn = {2008-823X}, year = {2000} } @article{ author = {Abolhassani, mohse}, title = {Identification of Two Epitopes on the Outer Surface Protein A of the Lyme Disease Spirochete Borrelia burgdorferi}, abstract ={A murine IgM monoclonal antibody (MA-2C6) with κ-light chains directed against an antigenic determinant of outer surface protein A (OspA) of the Lyme disease spirochete, Borreliaburgdorferi, is produced. This antibody could bind specifically to OspA antigen of several isolates of B. burgdorferi, but not to the non-Lyme disease bacteria such as T. pallidum and B. hermsii. Antibody MA-2C6 was purified by ion-exchange chromatography and used for purification of OspA antigen from Borreliaburgdorferi cell lysate. This antibody together with an IgG1 monoclonal antibody specific for OspA, that was previously characterized, were used to test whether these antibodies recognize different epitopes on OspA antigen of Borreliaburgdorferi. For this test, ELISA double antibody binding was used. Two antibodies were added to the antigen either separately or simultaneously, and the amount of bound antibody was quantitatively measured by the use of rabbit anti-mouse IgG conjugated with alkaline phosphatase. Additivity of the bound enzymatic activity was observed when the monoclonal antibodies bind to distinct epitopes. With this test, two distinct epitopes were recognized on the OspA molecule. This antibody can be used not only for the purification and subtyping of OspA, but also for neutralization and immunotherapy.}, Keywords = {OspA, Anti-OspA, Epitopes, Lyme disease, Borrelia burgdorferi}, volume = {4}, Number = {1}, pages = {7-12}, publisher = {Pasteur Institute of Iran}, title_fa = {}, abstract_fa ={}, keywords_fa = {}, url = {http://ibj.pasteur.ac.ir/article-1-827-en.html}, eprint = {http://ibj.pasteur.ac.ir/article-1-827-en.pdf}, journal = {Iranian Biomedical Journal}, issn = {1028-852X}, eissn = {2008-823X}, year = {2000} } @article{ author = {Haghighi, Bahram and Yari, Mohamad and Tori, Shahraz}, title = {The Relationship between Cation-Induced Substrate Configuration and Enzymatic Activity of Phosphatidate Phosphohydrolase from Human Liver}, abstract ={The mechanism by which bi-and trivalent cations affect human liver phosphatidatephosphohydrolase (PAP) activity was investigated. Bivalent cations up to 1 mM increased PAP activity whereas at higher concentrations the activity of the enzyme decreased. The stimulatory concentration for trivalent cations such as Al3+ and Cr3+, however, was much lower being 2 m M and 1 m M, respectively. All cations affecting PAP activity were also able to induce phase transition of phosphatidate from lamellar (La) to inverted hexagonal (HII) form. The rate of La-HII transition was different for each cation. At 100 mM concentration of Mg2+ only 26% of the original phosphatidate remained in La form and for other cations tested ranged from 14.5% to 76%. The phase transition was blocked by EDTA. Magnesium from 0.8 to 1.5 mM concentration raised PAP activity (3-fold) with La form of substrate but not with the HII phase. Monovalent cations such as Na+ and K+ neither affected enzyme activity nor substrate configuration. These data suggest that cation-induced PAP activation is not as a result of cation-protein interaction, but is due to formation of a suitable substrate configuration for the enzyme catalysis during phosphatidate phase transition. It appears that the real substrate configuration for PAP activity is situated between La and HII phases.}, Keywords = {Phosphatidate, Phosphohydrolase, Phosphatidic acid}, volume = {4}, Number = {1}, pages = {13-19}, publisher = {Pasteur Institute of Iran}, title_fa = {}, abstract_fa ={}, keywords_fa = {}, url = {http://ibj.pasteur.ac.ir/article-1-829-en.html}, eprint = {http://ibj.pasteur.ac.ir/article-1-829-en.pdf}, journal = {Iranian Biomedical Journal}, issn = {1028-852X}, eissn = {2008-823X}, year = {2000} } @article{ author = {Naghdi, Nasser and Majlessi, Nahi}, title = {Effects of Citalopram on Learning and Memory in the Mouse and Rat}, abstract ={Data on the effects of serotonin reuptake inhibitors on learning and memory processes are not consistent. In the present study, the effects of citalopram, a very potent and completely selective inhibitor of the serotonin reuptake on spatial discrimination in the T-maze and Morris water maze, were assessed in mice and/or rats. Animals received different doses of citalopram (1, 2, 4, 8 or 16 mg/kg, i.p.) or its vehicle (saline) 30 min before training each day. The results showed no significant effects of citalopram on T-maze discrimination task in mice and rats. However, there was dose-dependent increases in latencies to find the invisible platform and traveled distances in citalopram received groups compared to the control group with the peak effect at doses of 4 and 8 mg/kg in Morris task. Therefore, it appears that citalopram can cause learning deficits in complex spatial tasks.}, Keywords = {Serotonin, Citalopram, Spatial learning}, volume = {4}, Number = {1}, pages = {21-29}, publisher = {Pasteur Institute of Iran}, title_fa = {}, abstract_fa ={}, keywords_fa = {}, url = {http://ibj.pasteur.ac.ir/article-1-830-en.html}, eprint = {http://ibj.pasteur.ac.ir/article-1-830-en.pdf}, journal = {Iranian Biomedical Journal}, issn = {1028-852X}, eissn = {2008-823X}, year = {2000} } @article{ author = {Zehtab, Tahere and Yazdanparast, Razieh and Rafieii, Shahnaz}, title = {Prevention of Experimental Autoimmune Vitiligo by Oral Administration of Mushroom Tyrosinase}, abstract ={Experimental autoimmune vitiligo was induced by the intradermal injection of the purified mushroom tyrosinase emulsified in Complete Freund’s Adjuvant (CFA) in female C57BL/6 mice. The onset of vitiligo was characterized by hair hypopigmentation and total melanocyte depletion in the basal layer of the epidermis. Oral administration of semipurified mushroom tyrosinase prevented experimental autoimmune vitiligo (EAV). Suppression of clinical and histological disease was observed when animals received mushroom tyrosinase. A decrease in lymphocyte proliferation, delayed type hypersensitivity responses, increase in humoral immunity of the mice may all suggest that the suppression of the disease is correlated with cellular immune responses suppression. Based on our data, it may be concluded that the oral administration of the mushroom tyrosinase may have practical implications in vitiligo.}, Keywords = {Mushroom tyrosinase, Experimental autoimmune vitiligo, Oral tolerance}, volume = {4}, Number = {1}, pages = {31-36}, publisher = {Pasteur Institute of Iran}, title_fa = {}, abstract_fa ={}, keywords_fa = {}, url = {http://ibj.pasteur.ac.ir/article-1-831-en.html}, eprint = {http://ibj.pasteur.ac.ir/article-1-831-en.pdf}, journal = {Iranian Biomedical Journal}, issn = {1028-852X}, eissn = {2008-823X}, year = {2000} } @article{ author = {Azizi, Mohammad and Musacchio, Alexis and Pardo, Ornaldo and Figueroa, Nelvis and Muzio, Vere}, title = {Expression of Hepatitis B Virus Core Antigen in Native and Fusion Forms in E. coli}, abstract ={DNA coding for the core antigen from hepatitis B Virus (HBcAg) was amplified, cloned and propagated in E. coli. The core protein was expressed in E. coli and the product was readily detected by Western blot. This protein can be used as a diagnostic material in serum screening tests. To increase the level of expression of this antigen in bacteria, two plasmids were constructed in which the gene coding for N-terminal part of the human IL-2 has been fused to 5¢ -terminus of the core antigen gene under control of the tryptophan promoter. Although the expression level of core antigen from hepatitis B Virus in E. coli was increased in fusion forms, but the size of fusion partner could affect the antigenicity, particle formation and assembly of the core antigen. The native and the two fusion forms of the core antigens from hepatitis B Virus were evaluated as a diagnostic material in serum screening.}, Keywords = {Hepatitis B core antigen, HBcAg, HBV, Expression}, volume = {4}, Number = {1}, pages = {37-43}, publisher = {Pasteur Institute of Iran}, title_fa = {Expression of Hepatitis B Virus Core Antigen in Native and Fusion Forms in E. coli}, abstract_fa ={}, keywords_fa = {}, url = {http://ibj.pasteur.ac.ir/article-1-832-en.html}, eprint = {http://ibj.pasteur.ac.ir/article-1-832-en.pdf}, journal = {Iranian Biomedical Journal}, issn = {1028-852X}, eissn = {2008-823X}, year = {2000} } @article{ author = {Behnam-Rasouli, Morteza and Nikravesh, Mohammad Reza and Mhadavi-Shahri, Naser and Tehranipour, Maryam}, title = {Post-Operative Time Effects after Sciatic Nerve Crush on the Number of Alpha Motoneurons, Using a Sterological Counting Method (Disector)}, abstract ={There are extensive evidences that show axonal processes of the nervous system (peripheral and/or central) may be degenerated after nerve injuries. Wallerian degeneration and chromatolysis are the most conspicuous phenomena that occur in response to injuries. In this research, the effects of post-operative time following sciatic nerve crush on the number of spinal motoneurons were investigated. Twelve adult male Wistar rats, whose left sciatic nerves were highly compressed for 30 s, assigned to experimental groups 1 and 2 (n = 6). After 3 and 8 weeks post-operative (in groups 1 and 2 respectively) the lumbar segments of spinal cord were sampled, processed, sectioned serially and stained with toluidine blue (pH 4.65). By using stereological quantitative technique (physical disector), the number of alpha motoneurons in the right and the left ventral horns of spinal cord were counted and compared with each other. Statistical analyses showed a remarkable reduction in the number of alpha motoneurons in the left side (experimental or operated) when compared with the right side (control or unoperated) both in 3 and 8 weeks post-operative groups. This reduction may be due to the blockade of retrograde axonal transport.}, Keywords = {Peripheral nerves, Neuronal degeneration, Alpha motoneurons, Stereology, Chromatolysis}, volume = {4}, Number = {1}, pages = {45-49}, publisher = {Pasteur Institute of Iran}, title_fa = {}, abstract_fa ={}, keywords_fa = {}, url = {http://ibj.pasteur.ac.ir/article-1-833-en.html}, eprint = {http://ibj.pasteur.ac.ir/article-1-833-en.pdf}, journal = {Iranian Biomedical Journal}, issn = {1028-852X}, eissn = {2008-823X}, year = {2000} } @article{ author = {K.Ardestani, Sussan and Amani, Mogtaba and kariminia, Ami}, title = {Restorative Effect of Vitamin E on some Immunological Parameters of Sub-Lethal γ-Irradiated BALB/c Mice}, abstract ={Elevated amount of the free radicals due to ionizing radiation causes deteriorating damage on immune system. Therefore, we made attempts to investigate the protective effect of vitamin E (vit-E), a biological antioxidant in BALB/c mice, so as to find an affordable prophylactic supplementation for individuals who are at risk. Several groups of mice were selected and exposed to sub-lethal g -irradiation with or without vit-E supplementation. At the end of exposure, mice were immunized by either live attenuated Brucellamelitensis vaccine or sheep red blood cell (SRBC). Consequently, the following parameters were assayed: specific antibody response, delayed type hypersensitivity and lymphocyte proliferation. We showed that vit-E supplementation restored the immune response in g -irradiated mice. These findings might have implications for individuals who are at risk of exposure to ionizing radiation.}, Keywords = {Vitamin E, Immune response, γ -Irradiation, BALB/c mice}, volume = {4}, Number = {1}, pages = {51-55}, publisher = {Pasteur Institute of Iran}, title_fa = {}, abstract_fa ={}, keywords_fa = {}, url = {http://ibj.pasteur.ac.ir/article-1-861-en.html}, eprint = {http://ibj.pasteur.ac.ir/article-1-861-en.pdf}, journal = {Iranian Biomedical Journal}, issn = {1028-852X}, eissn = {2008-823X}, year = {2000} }