@article{ author = {ZareeMahmodabady, Ali and Javadi, Hamid Reza and Kamali, Mehdi and Najafi, Ali and Hojati, Zahr}, title = {Bcr-abl Silencing by Specific Small-Interference RNA Expression Vector as a Potential Treatment for Chronic Myeloid Leukemia}, abstract ={Background: RNA interference (RNAi) is the mechanism of gene silencing-mediated messenger RNA degradation by small interference RNA (siRNA), which becomes a powerful tool for in vivo research, especially in the areas of cancer. In this research, the potential use of an expression vector as a specific siRNA producing tool for silencing of Bcr-abl in K562 cell line has been investigated. Methods: siRNA specific for Bcr-abl as short hairpin RNA (shRNA) was designed and cloned in expression vector (pRNAH1.1/Neo). K562 cells were cultured in RPMI media and transfected with shRNA expressing vector using lipofectamin 2000. Successful transfection was confirmed by significant increase of enhanced green fluorescent protein (EGFP) levels in K562-treated cells with expression vector (pEGFP-C1). In vitro studies in human K562 cell line entailed modulation of endogenous Bcr-abl mRNA levels which induced apoptosis. Effects of siRNA treatment on K562 cells were measured by ELISA. Results: Successful expression of siRNA was confirmed by significant reduction of Bcr-abl mRNA levels in K562 cells treated with expression vector (pRNAH1.1/Neo). siRNA directed against Bcr-abl effectively induced apoptosis and reduced viability in human K562 cell lines. Conclusion: Expression vector of siRNA can be used in vitro to target specific RNA and to reduce the levels of the specific gene product in the targeted cells. Results of this work suggest that RNAi has potential application for the treatment of a variety of diseases, including those involving abnormal gene expression and viral contamination.}, Keywords = {Apoptosis, SiRNA, K562 cell line, Bcr-abl}, volume = {14}, Number = {1}, pages = {1-8}, publisher = {Pasteur Institute of Iran}, title_fa = {Bcr-abl Silencing by Specific Small-Interference RNA Expression Vector as a Potential Treatment for Chronic Myeloid Leukemia}, abstract_fa ={}, keywords_fa = {}, doi = {-}, url = {http://ibj.pasteur.ac.ir/article-1-430-en.html}, eprint = {http://ibj.pasteur.ac.ir/article-1-430-en.pdf}, journal = {Iranian Biomedical Journal}, issn = {1028-852X}, eissn = {2008-823X}, year = {2010} } @article{ author = {Kheradmand, Fatemeh and Nourmohammadi, Issa and Modarressi, Mohammad Hossein and Firoozrai, Mohsen and Ahmadi-Faghih, Mohammad Ami}, title = {Differential Gene-Expression of Metallothionein 1M and 1G in Response to Zinc in Sertoli TM4 Cells}, abstract ={Background: Zinc (Zn) as an important trace element is essential for testicular development and spermatogenesis. Molecular mechanism of Zn action in the reproductive system may be related to metal binding low-molecular weight proteins, metallothioneins (MT). Our objective was to determine the effect of Zn on two important isoforms of MT, MT1M and MT1G genes expression on testicular sertoli cells. Methods: Cultured sertoli TM4 cells were exposed to different concentrations of Zn at different time points. Cellular uptake of Zn was tested using flame atomic absorption spectrometry. The cellular viability and gene expression were assessed by MTT and real-time PCR methods, respectively. Results: The treated cells resulted in higher Zn concentration and cellular viability. The expression of MT1M and MT1G genes in the treated cells were greater than those of the untreated cells (P<0.05). In the high dosage treated group (100 and 500 μM), Zn concentration and expression of MT1M and MT1G genes increased three h after treatment MT1G gene expression increased more at sixth h. At 18th h of treatment, the expression of both genes especially MT1G, increased dramatically while Zn concentration decreased. Conclusion: Since the increase of MT1G mRNA was coincident with cellular Zn level, it seems that MT1G has a more prominent role than MT1M in the homeostasis of Zn. In addition, Zn at dosage of 50 μM (pharmacologic concentration) may protect cells by increasing the expression of MT genes at longer periods.}, Keywords = {Gene expression, Metallothionein (MT), Sertoli cells, Zinc}, volume = {14}, Number = {1}, pages = {9-15}, publisher = {Pasteur Institute of Iran}, title_fa = {Differential Gene-Expression of Metallothionein 1M and 1G in Response to Zinc in Sertoli TM4 Cells}, abstract_fa ={}, keywords_fa = {}, doi = {-}, url = {http://ibj.pasteur.ac.ir/article-1-431-en.html}, eprint = {http://ibj.pasteur.ac.ir/article-1-431-en.pdf}, journal = {Iranian Biomedical Journal}, issn = {1028-852X}, eissn = {2008-823X}, year = {2010} } @article{ author = {Najafzadeh, Hossein and Safaeian, Leila and MirmohammadSadeghi, Hamid and Rabbani, Mohammad and Jafarian, Abbas}, title = {The Effect of Aspartate-Lysine-Isoleucine and Aspartate-Arginine-Tyrosine Mutations on the Expression and Activity of Vasopressin V2 Receptor Gene}, abstract ={Background: Vasopressin type 2 receptor (V2R) plays an important role in the water reabsorption in the kidney collecting ducts. V2R is a G protein coupled receptor (GPCR) and the triplet of amino acids aspartate-arginine-histidine (DRH) in this receptor might significantly influence its activity similar to other GPCR. However, the role of this motif has not been fully confirmed. Therefore, the present study attempted to shed some more light on the role of DRH motif in G protein coupling and V2R function with the use of site-directed mutagenesis. Methods: Nested PCR using specific primers was used to produce DNA fragments containing aspartate-lysine-isoleucine and aspartate-arginine-tyrosine mutations with replacements of the arginine to lysine and histidine to tyrosine, respectively. After digestion, these inserts were ligated into the pcDNA3 vector and transformation into E. coli HB101 was performed using heat shock method. The obtained colonies were analyzed for the presence and orientation of the inserts using proper restriction enzymes. After transient transfection of COS-7 cells using diethylaminoethyl-dextran method, the adenylyl cyclase activity assay was performed for functional study. The cell surface expression was analyzed by indirect ELISA method. Results: The functional assay indicated that none of these mutations significantly altered cAMP production and cell surface expression of V2R in these cells. Conclusion: Since some substitutions in arginine residue have shown to lead to the inactive V2 receptor, further studies are required to define the role of this residue more precisely. However, it seems that the role of the histidine residue is not critical in the V2 receptor function.}, Keywords = {Mutation, Polymerase chain reaction, Vasopressin receptor}, volume = {14}, Number = {1}, pages = {17-22}, publisher = {Pasteur Institute of Iran}, title_fa = {The Effect of Aspartate-Lysine-Isoleucine and Aspartate-Arginine-Tyrosine Mutations on the Expression and Activity of Vasopressin V2 Receptor Gene}, abstract_fa ={}, keywords_fa = {}, doi = {-}, url = {http://ibj.pasteur.ac.ir/article-1-432-en.html}, eprint = {http://ibj.pasteur.ac.ir/article-1-432-en.pdf}, journal = {Iranian Biomedical Journal}, issn = {1028-852X}, eissn = {2008-823X}, year = {2010} } @article{ author = {Nowrouzi, Azin and Meghrazi, Khadijeh and Golmohammadi, Taghi and Golestani, Abolfazl and Ahmadian, Shahin and Shafiezadeh, Mahshid and Shajary, Zahra and Khaghani, Shahnaz and Amiri, Azita N.}, title = {Cytotoxicity of Subtoxic AgNP in Human Hepatoma Cell Line (HepG2) after Long-Term Exposure}, abstract ={Background: We aimed at evaluating the toxicity effects of low (subtoxic) concentrations of silver nanoparticles (AgNP, 5-10 nm) in human hepatoblastoma (HepG2) cell line after and during a period of about one month. Methods: XTT and MTT assays were used to draw a dose-response curve IC50 (half maximal inhibitory concentration) value of the AgNP on HepG2 cells was calculated to be 2.75-3.0 mg/l. The cells were exposed to concentrations of 0% (control), 1%, 4% and 8% IC50 of AgNP (corresponding to 0.00, 0.03, 0.12 and 0.24 mg/l of AgNP, respectively) for four consecutive passages. The treated cells were compared to the control group with respect to morphology and proliferation at the end of the period. Results: The biochemical studies revealed significant increases of lactate dehydrogenase and alanine aminotransferase enzyme activity in the culture media of cells receiving 4% and 8% IC50 the increases in the aspartate aminotransferase enzyme activity and nitric oxide concentration became significant at 8% IC50. In the cell extracts, the average total protein and activity of glutathione peroxidase enzyme remained unchanged the decrease in the average content of glutathione (GSH) and superoxide dismutase (SOD) activity became significant at 4% and 8% IC50. There were increases in lipid peroxidation (significant at 4% and 8% IC50) and cytochrome c content (significant at 8% IC50). The accumulations of the effects, during the experiment from one generation to the next, were not statistically remarkable except in cases of GSH and SOD. The results indicate clearly the involvement of oxidative changes in the cells after exposure to low doses of AgNP. Conclusion: The results might help specify a safer amount of AgNP for use in different applications.}, Keywords = {Cytotoxicity, Liver, Enzyme activity}, volume = {14}, Number = {1}, pages = {23-32}, publisher = {Pasteur Institute of Iran}, title_fa = {Cytotoxicity of Subtoxic AgNP in Human Hepatoma Cell Line (HepG2) after Long-Term Exposure}, abstract_fa ={}, keywords_fa = {}, doi = {-}, url = {http://ibj.pasteur.ac.ir/article-1-433-en.html}, eprint = {http://ibj.pasteur.ac.ir/article-1-433-en.pdf}, journal = {Iranian Biomedical Journal}, issn = {1028-852X}, eissn = {2008-823X}, year = {2010} } @article{ author = {Mirshekar, Mohammadali and Roghani, Mehrdad and Khalili, Mohsen and Baluchnejadmojarad, Tourandokht and ArabMoazzen, Saiedeh}, title = {Chronic Oral Pelargonidin Alleviates Streptozotocin-Induced Diabetic Neuropathic Hyperalgesia in Rat: Involvement of Oxidative Stress}, abstract ={Background: Diabetes mellitus in some clinical cases is accompanied with hyperalgesia. In this study, we evaluated the possible beneficial effect of chronic pelargonidin (PG) treatment on hyperalgesia in streptozotocin (STZ)-diabetic neuropathic rat. Methods: Male Wistar rats (n = 56) were divided into seven groups, i.e. control, diabetic, PG-treated control, PG (single- and multiple-dose)-treated diabetic, and sodium salicylate-treated control and diabetics. For induction of diabetes, STZ was injected i.p. at a single dose of 60 mg/kg. PG was orally administered at a dose of 10 mg/kg once and/or on alternate days for 8 weeks 1 week after diabetes induction. After two months, hyperalgesia was assessed using standard formalin and hot tail immersion tests. Meanwhile, markers of oxidative stress in brain were measured. One-way analysis of variance was used for statistical analysis of the data. Results: Diabetic rats showed a marked chemical and thermal hyperalgesia, indicating that development of diabetic neuropathy and PG treatment (especially multiple-doses) significantly ameliorated the alteration in hyperalgesia (P<0.05-0.01) in diabetic rats as compared to untreated diabetics. PG (multiple doses) also significantly decreased diabetes-induced thiobarbituric acid reactive substances formation and non-significantly reversed elevation of nitrite level and reduction of antioxidant defensive enzyme superoxide dismutase. Conclusion: These results clearly suggest that PG prevents diabetic neuropathic hyperalgesia through attenuation of oxidative stress.}, Keywords = {Pelargonidin (PG), Streptozotocin (STZ), Oxidative stress}, volume = {14}, Number = {1}, pages = {33-39}, publisher = {Pasteur Institute of Iran}, title_fa = {Chronic Oral Pelargonidin Alleviates Streptozotocin-Induced Diabetic Neuropathic Hyperalgesia in Rat: Involvement of Oxidative Stress}, abstract_fa ={}, keywords_fa = {}, doi = {-}, url = {http://ibj.pasteur.ac.ir/article-1-434-en.html}, eprint = {http://ibj.pasteur.ac.ir/article-1-434-en.pdf}, journal = {Iranian Biomedical Journal}, issn = {1028-852X}, eissn = {2008-823X}, year = {2010} } @article{ author = {Shah, Saloni P. and Gohil, Priyanshee V. and Shah, Gaurang B.}, title = {Role of Estrogen Receptor-Alpha in an Experimental Model of Bronchial Asthma}, abstract ={Background: Use of hormone replacement therapy (HRT) may increase the risk of adult-onset asthma in women. Various in vitro studies have reported that estradiol stimulates human mast cell lines causing release of allergic mediators which was not observed in estrogen receptor-alpha (ER-alpha) knockout mice. Thus, estrogen might be a key element in occurrence of asthma. In the present study, we proposed to determine the role of ER-alpha in an experimental model of bronchial asthma. Methods: Trypsin and egg albumin induced chronic model of asthma were used. On the 28th day, various parameters such as pO2 level, serum bicarbonate level, tidal volume, respiratory rate, air flow rate, differential white blood cells count in the bronchoalveolar lavage (BAL) fluid and serum cholesterol level were measured as well as lung histopathological examination and uterine weight measurement were carried out. Results: Estradiol treatment resulted in lower pO2 level, tidal volume and air flow rate. Also, serum bicarbonate level, respiratory rate and eosinophil rate and eosinophil count in BAL fluid were higher as compared to asthmatic control group. These effects were not observed in methyl-piperidino-pyrazole (MPP) co-treated group. Histopathological data suggested that the destruction of alveolar and muscular layers was more prominent in estradiol-treated group than asthmatic control and MPP co-treated groups. Estradiol-treated group showed lower total serum cholesterol levels and higher uterine weight as compared to asthmatic control group which was not observed in MPP co-treated group indicating antagonism of estradiol by MPP at ER-alpha receptor. Conclusion: Estrogen seems to have a strong promoting effect on pathogenesis of bronchial asthma via ER-alpha receptors.}, Keywords = {Estrogen receptor Alpha (ER-α), Estradiol, Asthma}, volume = {14}, Number = {1}, pages = {41-48}, publisher = {Pasteur Institute of Iran}, title_fa = {Role of Estrogen Receptor-Alpha in an Experimental Model of Bronchial Asthma}, abstract_fa ={}, keywords_fa = {}, doi = {-}, url = {http://ibj.pasteur.ac.ir/article-1-435-en.html}, eprint = {http://ibj.pasteur.ac.ir/article-1-435-en.pdf}, journal = {Iranian Biomedical Journal}, issn = {1028-852X}, eissn = {2008-823X}, year = {2010} } @article{ author = {Lashkarian, Hamed and Raheb, Jamshid and Shahzamani, Kiana and Shahbani, Hossein and Shamsara, Mehdi}, title = {Extracellular Cholesterol Oxidase from Rhodococcus sp.: Isolation and Molecular Characterization}, abstract ={Background: Cholesterol oxidase (CHO) has various clinical and industrial applications. Recently, microbial CHO have received a great attention for their wide usage in medicine. Here, taxonomic characterizations of isolated strain from soil, optimization of the conditions for CHO production and biochemical characterizations of produced CHO enzyme were described. Finally, CHO gene was cloned into a cloning vector. Methods: Various samples were collected and cultivated in a screening medium consisting of cholesterol. For isolation of CHO-producing bacteria, well-grown colonies were inoculated into an optimized medium. Different biochemical and microbiological tests were performed on isolated bacteria to identify their properties. For phylogenic analysis, a partial sequence of l6s rRNA was amplified by PCR using universally conserved primers. A modified method was applied for determination of CHO activity. Then, extracellular CHO activity was assessed under different temperature, pH and cholesterol concentration conditions. Finally, CHO gene was amplified by PCR and cloned into STV28. Results: According to the morphological, cultural and biochemical tests, the isolated bacterium was identified as Rhodococcus sp. strain 501 and deposited in GenBank with accession number FN298676. Results showed that optimum temperature and pH for CHO activity were 35°C and 7.5, respectively. Alignment of nucleotide sequence of CHO gene showed 99% homology with other bacterial CHO genes. Conclusion: Rhodococcus sp. strain 501 produced significant levels of extracellular CHO in an optimized medium for a short period. CHO gene was cloned into cloning vector that can be a valuable tool for better identification and further studies on gene expression.}, Keywords = {Rhodococcus, Cholesterol oxidase, Soil}, volume = {14}, Number = {1}, pages = {49-57}, publisher = {Pasteur Institute of Iran}, title_fa = {Extracellular Cholesterol Oxidase from Rhodococcus sp.: Isolation and Molecular Characterization}, abstract_fa ={}, keywords_fa = {}, doi = {-}, url = {http://ibj.pasteur.ac.ir/article-1-436-en.html}, eprint = {http://ibj.pasteur.ac.ir/article-1-436-en.pdf}, journal = {Iranian Biomedical Journal}, issn = {1028-852X}, eissn = {2008-823X}, year = {2010} } @article{ author = {Khalili, Mohsen and Hamzeh, Faezeh}, title = {Effects of Active Constituents of Crocus sativus L., Crocin on Streptozocin-Induced Model of Sporadic Alzheimer\'s Disease in Male Rats}, abstract ={Background: The involvement of water-soluble carotenoids, crocins, as the main and active components of Crocus sativus L. extract in learning and memory processes has been proposed. In the present study, the effect of crocins on sporadic Alzheimer's disease induced by intracerebroventricular (icv) streptozocin (STZ) in male rats was investigated. Methods: Male adult Wistar rats (n = 90 and 260-290 g) were divided into 1, control 2 and 3, crocins (15 and 30 mg/kg) 4, STZ 5 and 6, STZ + crocins (15 and 30 mg/kg) groups. In Alzheimer's disease groups, rats were injected with STZ-icv bilaterally (3 mg/kg) in first day and 3 days later, a similar STZ-icv application was repeated. In STZ + crocin animal groups, crocin was applied in doses of 15 and 30 mg/kg, i.p., one day pre-surgery and continued for three weeks. Prescription of crocin in each dose was repeated once for two days. However, the learning and memory performance was assessed using passive avoidance paradigm, and for spatial cognition evaluation, Y-maze task was used. Results: It was found out that crocin (30 mg/kg)-treated STZ-injected rats show higher correct choices and lower errors in Y-maze than vehicle-treated STZ-injected rats. In addition, crocin in the mentioned dose could significantly attenuated learning and memory impairment in treated STZ-injected group in passive avoidance test. Conclusion: Therefore, these results demonstrate the effectiveness of crocin (30 mg/kg) in antagonizing the cognitive deficits caused by STZ-icv in rats and its potential in the treatment of neurodegenerative diseases such as Alzheimer's disease.}, Keywords = {Streptozocin, Learning, Memory}, volume = {14}, Number = {1}, pages = {59-65}, publisher = {Pasteur Institute of Iran}, title_fa = {Effects of Active Constituents of Crocus sativus L., Crocin on Streptozocin-Induced Model of Sporadic Alzheimer\'s Disease in Male Rats}, abstract_fa ={}, keywords_fa = {}, doi = {-}, url = {http://ibj.pasteur.ac.ir/article-1-437-en.html}, eprint = {http://ibj.pasteur.ac.ir/article-1-437-en.pdf}, journal = {Iranian Biomedical Journal}, issn = {1028-852X}, eissn = {2008-823X}, year = {2010} } @article{ author = {Shahidi, Maryam and Mozdarani, Hossein and Mueller, Wolfgang-Ulrich}, title = {Radiosensitivity and Repair Kinetics of Gamma-Irradiated Leukocytes from Sporadic Prostate Cancer Patients and Healthy Individuals Assessed by Alkaline Comet Assay}, abstract ={Background: Impaired DNA repair mechanism is one of the main causes of tumor genesis. Study of intrinsic radiosensitivity of cancer patients in a non-target tissue (e.g. peripheral blood) might show the extent of DNA repair deficiency of cells in affected individuals and might be used a predictor of cancer predisposition. Methods: Initial radiation-induced DNA damage (ratio of Tail DNA/Head DNA), dose-response curves and kinetics of DNA repair in leukocytes from healthy volunteers and prostate cancer patients were assessed using alkaline comet assay after exposure to 60Co gamma rays. Results: Results showed that higher levels of baseline and gamma rays induced DNA damage in leukocytes of prostate cancer cases than in controls. A similar dose response was obtained for both groups. After a repair time of 24 h following in vitro irradiation, samples from the healthy individuals showed no residual DNA damage in their leukocytes, whereas prostate cancer patients revealed more than 20%. Although similar initial radiosensitivity was observed for both groups, the repair kinetics of radiation induced DNA damage of leukocytes from prostate cancer cases and healthy subjects were statistically different. Conclusion: These results support the hypothesis that men affected by prostate cancer may have a constitutional genomic instability.}, Keywords = {Leukocytes, DNA damage , Radiosensitivity, Prostate cancer, Comet assay}, volume = {14}, Number = {3}, pages = {67-75}, publisher = {Pasteur Institute of Iran}, title_fa = {Radiosensitivity and Repair Kinetics of Gamma-Irradiated Leukocytes from Sporadic Prostate Cancer Patients and Healthy Individuals Assessed by Alkaline Comet Assay}, abstract_fa ={}, keywords_fa = {}, doi = {-}, url = {http://ibj.pasteur.ac.ir/article-1-438-en.html}, eprint = {http://ibj.pasteur.ac.ir/article-1-438-en.pdf}, journal = {Iranian Biomedical Journal}, issn = {1028-852X}, eissn = {2008-823X}, year = {2010} } @article{ author = {Rabbani, Hodjattallah and Ostadkarampour, Mahyar and DaneshManesh, Amir Hossein and Basiri, Abbas and Jeddi-Tehrani, Mahmood and Forouzesh, Flor}, title = {Expression of ROR1 in Patients with Renal Cancer-A Potential Diagnostic Marker}, abstract ={Background: The ectopic expression of receptor tyrosine kinase Ror1 has been reported in patients with hematological malignancies such as chronic lymphocytic leukemia and acute lymphoblastic leukemia. Here we report, for the first time, expression of ROR1 gene in both tumor tissues and peripheral blood mononuclear cells (PBMC) from patients with renal cancer (RC). Methods: In the current study, the expression of ROR1 gene was semi-quantitatively measured in PBMC and tumor tissues from 16 RC patients as well as PBMC from 22 healthy individuals relative to the expression of the housekeeping gene phosphoglucomutase 1 by RT-PCR. Results: Our results showed that ROR1 was expressed at gene level in 81.3% of renal tumor tissues (13 out of 16) whereas it was expressed in 94% of PBMC from RC patients (15 out of 16). A weak expression of ROR1 was observed in PBMC of 4 out of 22 healthy individual. A significant expression of ROR1 was observed in PBMC from RC patients when compared to that in PBMC from normal healthy individuals (P<0.001). The expression of ROR1 in PBMC may reflect a shedding of tumor cells into blood stream. Conclusion: We conclude that detection of a high level of ROR1 expression in blood cells might assist in early detection of renal malignancies, providing taking into consideration the clinical symptoms of the disease.}, Keywords = {ROR1, Ectopic expression, Renal cancer}, volume = {14}, Number = {3}, pages = {77-82}, publisher = {Pasteur Institute of Iran}, title_fa = {Expression of ROR1 in Patients with Renal Cancer-A Potential Diagnostic Marker}, abstract_fa ={}, keywords_fa = {}, doi = {-}, url = {http://ibj.pasteur.ac.ir/article-1-439-en.html}, eprint = {http://ibj.pasteur.ac.ir/article-1-439-en.pdf}, journal = {Iranian Biomedical Journal}, issn = {1028-852X}, eissn = {2008-823X}, year = {2010} } @article{ author = {Khansarinejad, Behzad and Soleimanjahi, Hoorieh and Ghaemi, Amir and Tiraihi, Taki and PourBeiranvand, Shahram}, title = {Localization of Herpes Simplex Virus Type 1 DNA in Latently Infected BALB/c Mice Neurons Using in situ Polymerase Chain Reaction}, abstract ={Background: Herpes simplex virus type-1 (HSV-1) establishes a lifelong latent infection in neurons following primary infection. The existence of latent HSV-1 DNA in the trigeminal ganglia of infected BALB/c mice was examined using a direct in situ PCR technique, based on Digoxigenin-11-dUTP detection system with anti-digoxigenin-peroxidase and 3,3'-diaminobenzidine (DAB) substrate. Methods: Eight-week-old male BALB/c mice were inoculated via the eye by 104 plaque forming unit of wild type Iranian isolates of HSV-1. After establishment of latency, trigeminal ganglia were removed and examined using in situ PCR to detect HSV-1 genome. Finally, the results of in situ PCR were verified by a two-round PCR method, using amplification cocktail of in situ reaction, as a template for a conventional gel base PCR. Results and Conclusion: The results suggest that a direct in situ PCR method using a peroxidase and DAB detection system is a useful means for detection of latent HSV-1 DNA in the latently infected ganglia.}, Keywords = {Herpes simplex virus-1, Latency, In situ PCR, two-round PCR, Trigeminal ganglia}, volume = {14}, Number = {3}, pages = {83-88}, publisher = {Pasteur Institute of Iran}, title_fa = {Localization of Herpes Simplex Virus Type 1 DNA in Latently Infected BALB/c Mice Neurons Using in situ Polymerase Chain Reaction}, abstract_fa ={}, keywords_fa = {}, doi = {-}, url = {http://ibj.pasteur.ac.ir/article-1-440-en.html}, eprint = {http://ibj.pasteur.ac.ir/article-1-440-en.pdf}, journal = {Iranian Biomedical Journal}, issn = {1028-852X}, eissn = {2008-823X}, year = {2010} } @article{ author = {Maleki, Mehri and Sayyah, Mohammad and Kamgarpour, Fatemeh and Karimipoor, Morteza and Arab, Aida and Rajabi, Anahita and Gharagozli, Kourosh and Shamshiri, Ahmad Reza and ShahsavandAnanloo, Esmaeil}, title = {Association between ABCB1-T1236C Polymorphism and Drug-Resistant Epilepsy in Iranian Female Patients}, abstract ={Background: One third of epileptic patients are resistant to several anti-epileptic drugs (AED). P-glycoprotein (P-gp) is an efflux transporter encoded by ATP-binding cassette subfamily B member 1 (ABCB1) gene that excludes drugs from the cells and plays a significant role in AEDs resistance. Over-expression of P-gp could be a result of polymorphisms in ABCB1 gene. We studied the association of T129C and T1236C single-nucleotide polymorphisms (SNP) of ABCB1 gene with drug-resistant epilepsy in Iranian epileptics. Methods: DNA samples were obtained from 200 healthy controls and 332 epileptic patients, of whom 200 were drug responsive and 132 drug resistant. The frequencies of the genotypes of the two SNP were determined by polymerase chain reaction followed by restriction fragment length polymorphism. Results: No significant association was found between T129C and T1236C genotypes and drug-resistant epilepsy neither in adults nor in children. However, the risk of drug resistance was higher in female patients with 1236CC (P = 0.02) or CT (P = 0.008) genotype than in those with TT genotype. The risk of drug resistance was also higher in patients with symptomatic epilepsies with 1236CC (P = 0.02) or CT (P = 0.004) genotype than in those with TT genotype. The risk of drug resistance was lower in patients with idiopathic epilepsies with 129TT genotype (P = 0.001) than in those with CT genotype. Conclusion: Our results indicate that T1236C polymorphism is associated with drug resistance in Iranian female epileptic patients. Replication studies with large sample sizes are needed to confirm our results.}, Keywords = {ATP-binding cassette subfamily B member 1 (ABCB1), Drug-resistant epilepsy, Single nucleotide polymorphism}, volume = {14}, Number = {3}, pages = {89-96}, publisher = {Pasteur Institute of Iran}, title_fa = {Association between ABCB1-T1236C Polymorphism and Drug-Resistant Epilepsy in Iranian Female Patients}, abstract_fa ={}, keywords_fa = {}, doi = {-}, url = {http://ibj.pasteur.ac.ir/article-1-441-en.html}, eprint = {http://ibj.pasteur.ac.ir/article-1-441-en.pdf}, journal = {Iranian Biomedical Journal}, issn = {1028-852X}, eissn = {2008-823X}, year = {2010} } @article{ author = {Kazemi, Bahram and Tohidi, Farideh and Bandehpour, Mojgan and Yarian, Fatemeh}, title = {Molecular Cloning, Expression and Enzymatic Assay of Pteridine Reductase 1 from Iranian Lizard Leishmania}, abstract ={Background: Currently, there are no effective vaccines against leishmaniasis, and treatment using pentavalent antimonial drugs is occasionally effective and often toxic for patients. The PTR1 enzyme, which causes antifolate drug resistance in Leishmania parasites encoded by gene pteridine reductase 1 (ptr1). Since Leishmania lacks pteridine and folate metabolism, it cannot synthesize the pteridine moiety from guanine triphosphate. Therefore, it must produce pteridine using PTR1, an essential part of the salvage pathway that reduces oxidized pteridines. Thus, PTR1 is a good drug-target candidate for anti-Leishmania chemotherapy. The aim of this study was the cloning, expression, and enzymatic assay of the ptr1 gene from Iranian lizard Leishmania as a model for further studies on Leishmania. Methods: Promastigote DNA was extracted from the Iranian lizard Leishmania, and the ptr1 gene was amplified using specific primers. The PCR product was cloned, transformed into Escherichia coli strain JM109, and expressed. The recombinant protein (PTR1 enzyme) was then purified and assayed. Results: ptr1 gene was successfully amplified and cloned into expression vector. Recombinant protein (PTR1 enzyme) was purified using affinity chromatography and confirmed by Western-blot and dot blot using anti-Leishmania major PTR1 antibody and anti-T7 tag monoclonal antibody, respectively. The enzymatic assay was confirmed as PTR1 witch performed using 6-biopterin as a substrate and nicotinamide adenine dinucleotide phosphate as a coenzyme. Conclusion: Iranian lizard Leishmania ptr1 was expressed and enzymatic assay was performed successfully.}, Keywords = {Pteridine reductase 1 (PTR1), Leishmania, Gene expression}, volume = {14}, Number = {3}, pages = {97-102}, publisher = {Pasteur Institute of Iran}, title_fa = {Molecular Cloning, Expression and Enzymatic Assay of Pteridine Reductase 1 from Iranian Lizard Leishmania}, abstract_fa ={}, keywords_fa = {}, doi = {-}, url = {http://ibj.pasteur.ac.ir/article-1-442-en.html}, eprint = {http://ibj.pasteur.ac.ir/article-1-442-en.pdf}, journal = {Iranian Biomedical Journal}, issn = {1028-852X}, eissn = {2008-823X}, year = {2010} } @article{ author = {MohammadiRoushandeh, Amaneh and HajiHosseinlou, Hassan and Niknafs, Behrouz and Halabian, Raheleh and Mehdipour, Ahmad and HabibiRoudkenar, Mehryar}, title = {Effects of Leukemia Inhibitory Factor on gp130 Expression and Rate of Metaphase II Development during in vitro maturation of mouse oocyte}, abstract ={Background: Leukemia inhibitory factor (LIF) is a 45-56 kDa glycoprotein that has an important role in proliferation and embryo implantation. Its effect on oocyte maturation and how to exert the function remained to be elucidated. Methods: Immature mice superovulated with human menopausal gonadotropin and germinal vesicle (GV) oocytes were obtained from ovary 48 hours after. GV oocytes were cultured in tissue culture medium 199 with 0, 100, 500 and 1,000 U/ml LIF. Cumulus expansion and in vitro maturation (IVM) rate were assessed after culture. For reverse transcriptase PCR, total RNA from GV and metaphase II (MII) oocytes were extracted by Trizol reagent. The quantity and quality of RNA were determined by spectrophotometry and electrophoresis, respectively. Reverse transcription was performed by Super Script III reverse transcriptase with 1 µg of total RNA followed by DNase I treatment and heat inactivation. Expression of gp130 was determined by RT-PCR. Results: Our results showed that cumulus expansion was improved with 1,000 U/ml in culture medium compared to others. GV breakdown and MII rate in groups with LIF were higher than control group and were dose dependant. In 1,000 U/ml, LIF rate of MII was significantly higher than control group (P<0.05). Our results also showed that gp130 is expressed neither in GV nor in MII oocytes during IVM of mouse oocytes. Conclusion: gp130 is expressed in human oocyte but not in mouse. Our results suggest that in mouse, LIF could affect the oocyte via another receptor or via cumulus cells however, further studies are warranted.}, Keywords = {Leukemia inhibitory factor (LIF), Oocytes, In vitro maturation (IVM)}, volume = {14}, Number = {3}, pages = {103-107}, publisher = {Pasteur Institute of Iran}, title_fa = {Effects of Leukemia Inhibitory Factor on gp130 Expression and Rate of Metaphase II Development during in vitro}, abstract_fa ={}, keywords_fa = {}, doi = {-}, url = {http://ibj.pasteur.ac.ir/article-1-444-en.html}, eprint = {http://ibj.pasteur.ac.ir/article-1-444-en.pdf}, journal = {Iranian Biomedical Journal}, issn = {1028-852X}, eissn = {2008-823X}, year = {2010} } @article{ author = {Peyghambari, Fatemeh and Salehnia, Mojdeh and ForouzandehMoghadam, Mehdi and RezazadehValujerdi, Mojtaba and Hajizadeh, Ebrahim}, title = {The Correlation between the Endometrial Integrins and Osteopontin Expression with Pinopodes Development in Ovariectomized Mice in Response to Exogenous Steroids Hormones}, abstract ={Background: The ovariectomized animals are good models to evaluate the effect of different steroid hormone treatments on implantation events and the pattern of integrin expression. Therefore, this study was performed to compare the expression of integrins and osteopontin (OPN) in correlation with pinopode development in ovariectomized mice endometrium which was subjected to steroid hormones. Methods: Ovariectomized mice were subjected to estrogen, progesterone and estrogen-progesterone hormones. Their uterine horns were evaluated for integrin expression by immunohistochemistry and real-time RT-PCR and for pinopode development by transmission and scanning electron microscopic studies. Results: No immunostaining for integrin and OPN molecules were detected in the endometrium of non-ovariectomized mice except in metestrus phase. The α4 and β1 integrin genes were expressed in all phases of estrous cycle. In ovariectomized mice, no reaction was detected in the endometrium of control, sham and estrogen-treated groups, but in both progesterone-treated groups, all examined genes were expressed. There was not any correlation between pinopodes and integrin expression in ovariectomized mice. Conclusion: The progesterone is more effective on endometrial integrin expression than estrogen and differences in the expression pattern of integrins reflect their important and different roles in embryo implantation. The pinopodes may have minor effect in mice implantation or have some delay in their expressions in ovariectomized mice which were subjected to exogenous hormones.}, Keywords = {Endometrium, Estrogen, Integrin, Ovariectomized mice, Osteopontin (OPN)}, volume = {14}, Number = {3}, pages = {109-119}, publisher = {Pasteur Institute of Iran}, title_fa = {The Correlation between the Endometrial Integrins and Osteopontin Expression with Pinopodes Development in Ovariectomized Mice in Response to Exogenous Steroids Hormones}, abstract_fa ={}, keywords_fa = {}, doi = {-}, url = {http://ibj.pasteur.ac.ir/article-1-445-en.html}, eprint = {http://ibj.pasteur.ac.ir/article-1-445-en.pdf}, journal = {Iranian Biomedical Journal}, issn = {1028-852X}, eissn = {2008-823X}, year = {2010} } @article{ author = {Bokaeian, Mohammad and Nakhaee, Alireza and Moodi, Bita and Khazaei, Hossein Ali}, title = {Eucalyptus globulus (Eucalyptus) Treatment of Candidiasis in Normal and Diabetic Rats}, abstract ={Background: The leaves of Eucalyptus globulus (eucalyptus) are used for treatment of diabetes mellitus in traditional medicine. The aim of this study was to evaluate the effects of eucalyptus in treatment of established systemic infection with Candida albicans in normal and streptozotocin-induced diabetic rats. Methods: Sixty normoglycemic male Wistar rats, weighing 200-250 g, were selected and randomly divided into six groups (n= 10): normal control, control + C. albicans, control + eucalyptus + C. albicans, diabetic control, diabetic + C. albicans, diabetic + eucalyptus + C. albicans. Diabetes was induced after a single intraperitoneal injection of streptozotocin (60 mg/kg body weight) and eucalyptus was added to the diet (62.5 g/kg) and drinking water (2.5 g/L) of treated animals for 4 weeks. The concerned groups were inoculated with C. albicans 15 days after diabetes induction. At the end of one month experiment, fasted rats were killed by cervical decapitation. Blood was collected from neck vein for estimation of glucose. C. albicans concentrations were estimated in liver and kidneys using serial dilution culture of tissue homogenates. Results: Eucalyptus administration significantly improved the hyperglycemia, polydipsia, polyphagia, and it also compensated weight loss of diabetic rats (P<0.05). Moreover, eucalyptus caused a significant reduction in C. albicans concentration in liver and kidney homogenates (P<0.01). Conclusion: The results revealed that eucalyptus improves Candidia infection in normal and diabetic rats that in some ways validates the traditional use of this plant in treatment of diabetic patients.}, Keywords = {Diabetes mellitus, Eucalyptus globulus, Candida albicans}, volume = {14}, Number = {3}, pages = {121-126}, publisher = {Pasteur Institute of Iran}, title_fa = {Eucalyptus globulus (Eucalyptus) Treatment of Candidiasis in Normal and Diabetic Rats}, abstract_fa ={}, keywords_fa = {}, doi = {-}, url = {http://ibj.pasteur.ac.ir/article-1-446-en.html}, eprint = {http://ibj.pasteur.ac.ir/article-1-446-en.pdf}, journal = {Iranian Biomedical Journal}, issn = {1028-852X}, eissn = {2008-823X}, year = {2010} } @article{ author = {Taherian, Aliakbar and Ovsenek, Nick and Krone, Patrick H.}, title = {Expression of hsp90 Alpha and hsp90 Beta during Xenopus laevis Embryonic Development}, abstract ={Background: Members of the eukaryotic Hsp90 family function as important molecular chaperones in the assembly, folding and activation of cellular signaling in development. Two hsp90 genes, hsp90 alpha and hsp90 beta, have been identified in fish and homeothermic vertebrates but not in poikilothermic vertebrates. In the present study, the expression of hsp90 alpha and hsp90 beta genes in Xenopus laevis, which is phylogenetically positioned between zebrafish and mammals, has been addressed. Methods: Partial Xenopus hsp90 alpha and hsp90 beta cDNA were identified and isolated using RT-PCR, and a full-length Xenopus hsp90 beta cDNA was isolated from an embryonic cDNA library. Northern-blot analysis was used to study the expression of hsp90 alpha and hsp90 beta genes in total RNA of the embryos and in situ hybridization was used to compare the expression of these genes with that of hsp70 and MyoD genes in Xenopus embryogenesis. Results: Northern-blot analysis revealed that the hsp90 beta gene was strongly expressed constitutively at all stages of embryogenesis, but weakly induced following the heat shock. In contrast, the hsp90 alpha gene was weakly expressed in embryos at control temperature, but strongly up-regulated following heat shock. In situ hybridization results showed that hsp90 alpha gene was observed predominantly in cells of the developing somite. Microscopic sections showed that hsp90 alpha and MyoD mRNA are expressed in similar regions in somite and this pattern was distinct from that of hsp70 and hsp90 beta. Conclusion: These data support the hypothesis that the presence of hsp90 alpha and hsp90 beta genes is conserved among vertebrates, and these genes are differentially regulated in a tissue, stress, and development stage-specific manner.}, Keywords = {Xenopus, hsp90 alpha, hsp90 beta}, volume = {14}, Number = {4}, pages = {127-135}, publisher = {Pasteur Institute of Iran}, title_fa = {hsp90α and hsp90β Expression during Xenopus laevis Embryonic Development}, abstract_fa ={}, keywords_fa = {}, doi = {-}, url = {http://ibj.pasteur.ac.ir/article-1-447-en.html}, eprint = {http://ibj.pasteur.ac.ir/article-1-447-en.pdf}, journal = {Iranian Biomedical Journal}, issn = {1028-852X}, eissn = {2008-823X}, year = {2010} } @article{ author = {Oloomi, Mana and Bouzari, Saeid and Shariati, Elaheh}, title = {In vivo Characterization of Fusion Protein Comprising of A1 Subunit of Shiga Toxin and Human GM-CSF: Assessment of Its Immunogenicity and Toxicity}, abstract ={Background: Most cancer cells become resistant to anti-cancer agents. In the last few years, a new approach for targeted therapy of human cancer has been developed using immunotoxins which comprise both the cell targeting and the cell killing moieties. Methods: In the present study, the recombinant Shiga toxin A1 subunit fused to human granulocyte-macrophage colony stimulating factor (A1-GM-CSF), previously produced in E. coli, was further characterized. Results: The recombinant protein could cause 50% cytotoxicity and induced apoptosis in cells bearing GM-CSF receptors. The non-specific toxicity of the fusion protein was assessed in C57BL/6 and BALB/c mice. No mortality was observed in either group of mice, with different concentration of fusion protein. Conclusion: The lymphocyte proliferation assay, induction of specific IgG response and a mixed (Th1/Th2) response were observed only in BALB/c mice. The mixed response in BALB/c mice (Th1/Th2) could be explained on the basis of the two components of the fusion protein i.e. A1 and GM-CSF.}, Keywords = {Cell line, Fusion protein, Toxicity}, volume = {14}, Number = {4}, pages = {136-141}, publisher = {Pasteur Institute of Iran}, title_fa = {In vivo Characterization of Fusion Protein Comprising of A1 Subunit of Shiga Toxin and Human GM-CSF: Assessment of Its Immunogenicity and Toxicity}, abstract_fa ={}, keywords_fa = {}, doi = {-}, url = {http://ibj.pasteur.ac.ir/article-1-448-en.html}, eprint = {http://ibj.pasteur.ac.ir/article-1-448-en.pdf}, journal = {Iranian Biomedical Journal}, issn = {1028-852X}, eissn = {2008-823X}, year = {2010} } @article{ author = {Bakhtiary, Mehrdad and Marzban, Mohsen and Mehdizadeh, Mehdi and Joghataei, Mohammad Taghi and Khoei, Samideh and PirhajatiMahabadi, Vahid and Laribi, Bahareh and Tondar, Mahdi and Moshkforoush, Arash}, title = {Comparison of Transplantation of Bone Marrow Stromal Cells (BMSC) and Stem Cell Mobilization by Granulocyte Colony Stimulating Factor after Traumatic Brain Injury in Rat}, abstract ={Background: Recent clinical studies of treating traumatic brain injury (TBI) with autologous adult stem cells led us to compare effect of intravenous injection of bone marrow mesenchymal stem cells (BMSC) and bone marrow hematopoietic stem cell mobilization, induced by granulocyte colony stimulating factor (G-CSF), in rats with a cortical compact device. Methods: Forty adult male Wistar rats were injured with controlled cortical impact device and divided randomly into four groups. The treatment groups were injected with 2 × 106 intravenous bone marrow stromal stem cell (n = 10) and also with subcutaneous G-CSF (n = 10) and sham-operation group (n = 10) received PBS and "bromodeoxyuridine (Brdu)" alone, i.p. All injections were performed 1 day after injury into the tail veins of rats. All cells were labeled with Brdu before injection into the tail veins of rats. Functional neurological evaluation of animals was performed before and after injury using modified neurological severity scores (mNSS). Animals were sacrificed 42 days after TBI and brain sections were stained by Brdu immunohistochemistry. Results: Statistically, significant improvement in functional outcome was observed in treatment groups compared with control group (P<0.01). mNSS showed no significant difference between the BMSC and G-CSF-treated groups during the study period (end of the trial). Histological analyses showed that Brdu-labeled (MSC) were present in the lesion boundary zone at 42nd day in all injected animals. Conclusion: In our study, we found that administration of a bone marrow-stimulating factor (G-CSF) and BMSC in a TBI model provides functional benefits.}, Keywords = {Stem cells, Injection, Traumatic brain injury (TBI)}, volume = {14}, Number = {4}, pages = {142-149}, publisher = {Pasteur Institute of Iran}, title_fa = {Comparison of Transplantation of Bone Marrow Stromal Cells (BMSC) and Stem Cell Mobilization by Granulocyte Colony Stimulating Factor after Traumatic Brain Injury in Rat}, abstract_fa ={}, keywords_fa = {}, doi = {-}, url = {http://ibj.pasteur.ac.ir/article-1-449-en.html}, eprint = {http://ibj.pasteur.ac.ir/article-1-449-en.pdf}, journal = {Iranian Biomedical Journal}, issn = {1028-852X}, eissn = {2008-823X}, year = {2010} } @article{ author = {Karami, Manizheh and KarimianAzimi, Mohsen and Zarrindast, Mohammad Reza and Khalaji, Zeinab}, title = {Verifying of Participation of Nitric Oxide in Morphine Place Conditioning in the Rat Medial Septum Using Nicotinamide Adenine Dinucleotide Phosphate-Diaphorase (NADPH-d)}, abstract ={Background: Role of nitric oxide (NO) in morphine-induced conditioned place preference (CPP) has already been proposed in the rat medial septum (MS), but no molecular evidence has been provided to clear this fact. Methods: Effects of intraseptal injections of L-arginine and/or NG-nitro-L-arginine methyl ester (L-NAME) on morphine place conditioning in Wistar rats were examined. Morphine (2.5-7.5 mg/kg) was injected s.c. using a three-day schedule of an unbiased place preference. All of the brain samples were examined histochemically by nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d), the main marker for NO activation. Results: Morphine induced a significant CPP in the rats. Single injections of L-arginine or L-NAME (0.3, 1.0 and 3.0 µg/rat) did not induce CPP. In addition, co-administration of morphine (5.0 mg/kg) with L-arginine or L-NAME (0.3, 1.0 and 3.0 µg/rat) did not affect morphine response. However, administration of L-arginine (0.3, 1.0 and 3.0 µg/rat) prior to morphine conditioning testing enhanced the expression of morphine response. Moreover, pre-injection of L-NAME (0.3, 1.0 and 3.0 µg/rat) to L-arginine (0.3 µg/rat) did not reverse the response to the agent. The expression of NADPH-d was observed in the rat brain samples treated by L-arginine. A decreased expression of NADPH-d was also observed in rats pre-injected by L-NAME. Conclusion: This finding strongly suggests that NO system in the rat MS has an impact on the expression of morphine rewarding, and that the NO participates in place conditioning induced of morphine.}, Keywords = {Morphine, Nitric oxide (NO), Conditioned place preference (CPP), Nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d)}, volume = {14}, Number = {4}, pages = {150-157}, publisher = {Pasteur Institute of Iran}, title_fa = {Verifying of Participation of Nitric Oxide in Morphine Place Conditioning in the Rat Medial Septum Using Nicotinamide Adenine Dinucleotide Phosphate-Diaphorase (NADPH-d)}, abstract_fa ={}, keywords_fa = {}, doi = {-}, url = {http://ibj.pasteur.ac.ir/article-1-450-en.html}, eprint = {http://ibj.pasteur.ac.ir/article-1-450-en.pdf}, journal = {Iranian Biomedical Journal}, issn = {1028-852X}, eissn = {2008-823X}, year = {2010} } @article{ author = {Shohani, Behnaz and Orazizadeh, Mahmoud and Hashemitabar, Mahmoud and Heinegard, Dick}, title = {Degradation of Extracellular Matrix Molecules in Interleukin-1 Alpha Treated Bovine Nasal Cartilage}, abstract ={Background: This work aimed to show and compare the degradation time of some of cartilage extracellular matrix components using an in vitro model for cartilage degradation induced by interleukin-1 alpha. It is known that elucidation of molecular events under Interleukin-1 alpha induction of bovine nasal cartilage could obtain useful data to understand more about involving mechanisms for tissue breakdown in joint disease. Methods: The cartilage was taken from an adult bovine in a local slaughterhouse. After removing the whole perichondrium, the equal 2 mm diameter pieces of bovine nasal cartilage were punched out and cultured in Dulbecco's modified Eagle's medium DMEM with or without 10 ng/ml Interleukin-1 alpha for 24 days. Each 3 days, the media were removed and exchanged by fresh media, and the removed media were stored in -20C. Sodium dodecyl sulphate/polyacrylamid-gel electrophoresis SDS-PAGE and Western-blot methods were used for analyzing the samples. Results: The first fragment of fibromodulin (FM) was seen at day 6 and further fragments were appeared at day 18. Cartilage oligomeric matrix protein (COMP) releasing was as a successive pattern during culture period and the first fragment was found at day 6. Collagen IX fragments were seen at day 9 and in a progressive pattern until the end of the study. Conclusion: This study shows that FM and COMP could be considered as the suitable candidates for studying the mechanisms that participate in the cartilage degradations.}, Keywords = {Interleukin-1 alpha, Bovine cartilage, Cartilage oligomeric matrix protein (COMP), Collagen IX, Fibromodulin (FM)}, volume = {14}, Number = {4}, pages = {158-163}, publisher = {Pasteur Institute of Iran}, title_fa = {Degradation of Extracellular Matrix Molecules in Interleukin-1 Alpha Treated Bovine Nasal Cartilage}, abstract_fa ={}, keywords_fa = {}, doi = {-}, url = {http://ibj.pasteur.ac.ir/article-1-451-en.html}, eprint = {http://ibj.pasteur.ac.ir/article-1-451-en.pdf}, journal = {Iranian Biomedical Journal}, issn = {1028-852X}, eissn = {2008-823X}, year = {2010} } @article{ author = {MoiniZanjani, Taraneh and Sabetkasaei, Masoumeh}, title = {Study of the Intraplantar Injection of Lidocaine and Morphine on Pain Perception and the Influence of Morphine Dependence and Withdrawal on Lidocaine-Induced Analgesia in Rats}, abstract ={Background: Morphine and lidocaine are known to influence the perception of pain. The present study sought to determine the influence of local administration of morphine on lidocaine-induced analgesia in morphine non-dependent (MND), morphine dependent (MD) and morphine withdrawal (MW) animals. Methods: Adult male Wistar rats were divided into four groups: Control, MND, MD and MW rats. Lidocaine (0.5, 1 and 2%) and morphine (200, 400 and 800 µg) were injected in the plantar surface of the right paw. MD animals received chronic oral morphine (0.1, 0.2, 0.3 and 0.4 mg/ml in their drinking water) for 20 days. Twenty four hours before experiment, the animals in the MW group were deprived of morphine in their drinking water (physical dependence was observed by precipitating an abstinence syndrome with naloxone 2 mg/kg i.p.). Analgesia was assessed using hot-plate apparatus. Results: Morphine (400 µg) and lidocaine (2%) produce local analgesia in MND group. In MND rats, non-analgesic doses of each drug (200 µg morphine and 1% lidocaine) were used in combination and produced analgesia. In MD animals, all doses of lidocaine produced analgesia, while in MW animals, it failed to produce analgesia. In this situation, local administration of morphine could eventually influence the analgesic effect of lidocaine. Conclusion: Opioid withdrawal is one of the most common problems in clinic. This study determined the analgesic effect of lidocaine in MW animals in which lidocaine had no analgesic effect. In this regard, local administration of morphine with combination of lidocaine could probably produce an effective analgesia.}, Keywords = {Morphine, Lidocaine, Local analgesia, Dependence, Withdrawal}, volume = {14}, Number = {4}, pages = {164-170}, publisher = {Pasteur Institute of Iran}, title_fa = {Study of the Intraplantar Injection of Lidocaine and Morphine on Pain Perception and the Influence of Morphine Dependence and Withdrawal on Lidocaine-Induced Analgesia in Rats}, abstract_fa ={}, keywords_fa = {}, doi = {-}, url = {http://ibj.pasteur.ac.ir/article-1-452-en.html}, eprint = {http://ibj.pasteur.ac.ir/article-1-452-en.pdf}, journal = {Iranian Biomedical Journal}, issn = {1028-852X}, eissn = {2008-823X}, year = {2010} } @article{ author = {BeigiBoroujeni, Mandana and Salehnia, Mojdeh and Khalatbary, Ali Reza and Pourbeiranvand, Shahram and BeigiBoroujeni, Nasim and Ebrahimi, Saedeh}, title = {Effect of Ovarian Stimulation on the Endometrial Apoptosis at Implantation Period}, abstract ={Background: Apoptosis is a process that plays an important role during early stage of implantation. The aim of this study was to investigate the incidence of apoptosis in mice endometrium after ovarian stimulation at implantation period. Methods: NMRI female mice were divided into two groups: 1) control group, which were rendered pseudopregnant by vaginal stimulation and 2) experimental group, which were stimulated using an intrapritoneal injection of 10 IU hMG followed by another injection of 10 IU hCG after 48 h. In the evening of the second injection, the mice were rendered pseudopregnant the same as control group. Samples were obtained from 1/3 middle part of uterine horns during implantation period. Apoptosis was assessed in two groups at implantation period using light and electron microscopic studies, TUNEL staining and semiquantitative RT-PCR. Results: Our morphological and ultrastructural results showed apoptosis in both groups, while TUNEL analysis showed that the percentage of TUNEL-positive cells was higher in stimulated group than in the control group (P≤0.05). The expression of P53, Fas and FasL mRNA was similar in two groups but Bax and Bcl2 were much higher in control group than in the stimulated group (P≤0.05). The ratio of Bax/Bcl2 expression was much higher in stimulated group than in the control group (P≤0.05). Conclusion: The ovarian stimulation could change the expression of some apoptosis-related genes and enhance the incidence of endometrial apoptosis at implantation period thus, it could affect on the implantation rate and endometrial receptivity.}, Keywords = {Apoptosis, Endometrium, Gene expression}, volume = {14}, Number = {4}, pages = {171-177}, publisher = {Pasteur Institute of Iran}, title_fa = {Effect of Ovarian Stimulation on the Endometrial Apoptosis at Implantation Period}, abstract_fa ={}, keywords_fa = {}, doi = {-}, url = {http://ibj.pasteur.ac.ir/article-1-453-en.html}, eprint = {http://ibj.pasteur.ac.ir/article-1-453-en.pdf}, journal = {Iranian Biomedical Journal}, issn = {1028-852X}, eissn = {2008-823X}, year = {2010} } @article{ author = {Mirghazanfari, Sayid Mahdi and Keshavarz, Mansoor and Nabavizadeh, Fatemeh and Soltani, Nepton and Kamalinejad, Mohamm}, title = {The Effect of “Teucrium polium L.” Extracts on Insulin Release from in situ Isolated Perfused Rat Pancreas in a Newly Modified Isolation Method: the Role of Ca2+ and K+ Channels}, abstract ={Background: “Teucrium polium L.” (TP) has been long recommended in Iranian folk medicine for its anti-diabetic activities. We attempt here to evaluate the effect of TP extract on insulin secretion in rat pancreas. Methods: Rat pancreas was isolated in situ and perfused with Krebs solution containing low glucose (LG, 2.8 mM) or high glucose (HG, 16.7 mM) as perfusate. The aqueous extract (Aq. E) and methanolic extract (Met. E) of TP aerial parts and two partition fractions of Met. E were added to perfusate to evaluate insulin release. Diazoxide (DZX) and verapamil (VPM) were also used for assessing the probable mechanism of the effects. In each experimental group, the peak and baseline of insulin levels in effluent samples were compared. The GC/MS analysis was carried out to detect active ingredients in the extracts. Results: Adding Met. E to the LG caused a significant increase (P<0.05) in insulin release from the basal level of 0.17 ± 0.05 µg/l to a peak value of 3.94 ± 1.29 µg/l. when Met. E was introduced to the HG, there was a further protracted stimulation of insulin release from 2.15 ± 1.35 µg/l to 6.16 ± 0.52 µg/l. Both DZX and VPM when added separately to the LG, led to inhibition of Met. E induced insulin secretion. The Aq. E and fractions had no significant effect on insulin secretion. Only in the Met. E, the component 5-hydroxy-4',7-dimethoxyflavone (apigenin-4',7-dimethylether) was detected. Conclusion: It can be concluded that the insulinotropic properties of TP extracts can be attributed to the presence of apigenin existing only in Met. E, but not in Aq. E and fractions. Moreover, certain types of K+ and Ca2+ channels take part in this effect.}, Keywords = {Teucrium extract, Pancreas, Perfusion, Insulin}, volume = {14}, Number = {4}, pages = {178-185}, publisher = {Pasteur Institute of Iran}, title_fa = {The Effect of “Teucrium polium L.” Extracts on Insulin Release from in situ Isolated Perfused Rat Pancreas in a Newly Modified Isolation Method: the Role of Ca2+ and K+ Channels}, abstract_fa ={}, keywords_fa = {}, doi = {-}, url = {http://ibj.pasteur.ac.ir/article-1-454-en.html}, eprint = {http://ibj.pasteur.ac.ir/article-1-454-en.pdf}, journal = {Iranian Biomedical Journal}, issn = {1028-852X}, eissn = {2008-823X}, year = {2010} }