@article{ author = {ZareiMahmoudabadi, Ali and BernardDrucker, david and Mandall, Nicky and O'Brien, Kevin and Theaker, Elizabeth}, title = {Isolation and Identification of Candida Species from the Oral Cavity Using CHROMagar Candida}, abstract ={CHROMagar Candida is a new chromogenic culture medium that is used for the isolation and direct identification of some of the most clinically important yeast pathogens on the basis of colony colour. The aim of the study was to isolate and identify Candida species from the oral cavity with CHROMagar Candida. Samples (158) from 68 orthodontic patients wearing upper removable orthodontic appliances and 51 oral medicine patients were cultured on CHROMagar Candida at 37C for 48 h. Isolates with green colonies in colour were all confirmed as C. albicans (53) by germ tube test and others were confirmed as C. parapsilosis (10) and C. glabrata (3) by Rapid ID 32 C kits. There were 11 cases of mixed yeast cultures in this study that were easily recognised using the medium. In conclusion, C. albicans is the most frequently isolated yeast from the oral cavity of both orthodontic and oral medicine patients. Moreover CHROMagar Candida is a useful culture medium for the isolation and direct identification of Candida species, especially mixed cultures.}, Keywords = {CHROMagar Candida, Candida albicans, Candida parapsilosis}, volume = {4}, Number = {2}, pages = {57-61}, publisher = {Pasteur Institute of Iran}, title_fa = {}, abstract_fa ={}, keywords_fa = {}, url = {http://ibj.pasteur.ac.ir/article-1-822-en.html}, eprint = {http://ibj.pasteur.ac.ir/article-1-822-en.pdf}, journal = {Iranian Biomedical Journal}, issn = {1028-852X}, eissn = {2008-823X}, year = {2000} } @article{ author = {Mosayyebi, Ghasem and Shokri, Fazel}, title = {Comparative Measurement of Rheumatoid Factor in Serum and Synovial Fluid of Rheumatoid Arthritis Patients by ELISA and Latex-Agglutination Test}, abstract ={Rheumatoid factors (RF) are autoantibodies with specificity for the Fc portion of human IgG. Production of RF is a characteristic feature of rheumatoid arthritis (RA) and is detectable in high titer in about 90% of these patients. In this study, we measured total IgM, IgA, IgMRF and IgARF in serum and synovial fluid of 45 RA patients by ELISA and the results were compared with those obtained by latex agglutination test (LAT). The results show that from 45 RA patients, 93% (43:45) and 78% (35:45) were seropositive in ELISA and LAT, respectively. The sensitivity limit of LAT was approximately 10 µg/ml, whereas less than 10 ng/ml of RF was detectable by ELISA. There was a highly significant correlation between the concentration of IgMRF and latex agglutination titer in both serum (r = 0.78, p<0.0005) and synovial fluid (r = 0.66, p<0.05). Less significant correlations were observed for IgARF in serum (r = 0.65, p<0.05). While the concentration of total IgM and IgA were significantly higher in serum compared to synovial fluid, this however, was not reflected in the IgMRF or IgARF titer. In summary, our findings suggest that employment of isotype specific ELISA is inevitable and necessary for the quantitative measurement of RF isotypes other than IgM, which may be of clinical importance for diagnosis of disease severity and extra-articular complications of RA.}, Keywords = {Rheumatoid factor, Rheumatoid arthritis, ELISA, Latex-agglutination test}, volume = {4}, Number = {2}, pages = {63-67}, publisher = {Pasteur Institute of Iran}, title_fa = {}, abstract_fa ={}, keywords_fa = {}, url = {http://ibj.pasteur.ac.ir/article-1-823-en.html}, eprint = {http://ibj.pasteur.ac.ir/article-1-823-en.pdf}, journal = {Iranian Biomedical Journal}, issn = {1028-852X}, eissn = {2008-823X}, year = {2000} } @article{ author = {Qujeq, Durdi and Ataei, Gholamrez}, title = {Effects of Dietary Chitosan on Serum Lipid and Lipoprotein Concentration in Rats}, abstract ={Because the ingestion of some types of dietary fibers has been shown to influence on the lipid and lipoprotein levels, it is possible that chitosan influences on lipid metabolism. In the present study, the effects of chitosan on the serum, liver lipid and lipoprotein concentrations in rat were investigated. Serum lipid level in the treatment groups were decreased compared to that of the control, cholesterol level [128.65 +/- 2.58 (mean +/- SD, n = 72) vs. 173.67 +/- 3.62, p<0.05] mg/dl, triglyceride level [62.83 +/- 2.73 (mean +/- SD) vs. 93.62 +/- 2.64, p<0.05] mg/dl, and low density lipoprotein-cholesterol level [108.35 +/- 2.41(mean +/- SD) vs. 156.49 +/- 2.37, p<0.05] mg/dl. In the chitosan treatment group, high density lipoprotein-cholesterol level was increased as compared to the control [187.39 +/- 2.74 (mean +/- SD) vs. 163.54 +/- 2.83, p<0.05] mg/dl. This work showed that the addition of chitosan to the diet of the rats significantly lowered the liver lipid in the treatment groups compared to that of the control, cholesterol level [31.53 +/- 1.26(mean +/- SD) vs. 64.42 +/- 2.38, p<0.05] mg/g, and triglyceride level [38.46 +/- 2.64 (mean +/- SD) vs. 53.24 +/- 2.45, p<0.05] mg/g. When chitosan fed at the 5% level, concentration of the serum cholesterol was reduced by 25.92% and triglyceride by 32.89%. The data presented here indicated possible usefulness of chitosan for the treatment of hyperlipidemia.}, Keywords = {Chitosan, Cholesterol, Lipoprotein}, volume = {4}, Number = {2}, pages = {69-73}, publisher = {Pasteur Institute of Iran}, title_fa = {}, abstract_fa ={}, keywords_fa = {}, url = {http://ibj.pasteur.ac.ir/article-1-824-en.html}, eprint = {http://ibj.pasteur.ac.ir/article-1-824-en.pdf}, journal = {Iranian Biomedical Journal}, issn = {1028-852X}, eissn = {2008-823X}, year = {2000} } @article{ author = {Rezaee, Abbas and BehzadianNejad, Qorban and Kebriaeezadeh, Abbas}, title = {Simulataneous Analysis of Trimethoprim and Sulphamethoxazole Drug Combinations in Dosage Forms by High Performance Liquid Chromatography}, abstract ={A sensitive high performance liquid chromathography (HPLC) analytical procedure was developed for the quantitive determination of trimethoprim (TM* and sulphamethoxazole (SM) in commercial dosage forms. C18 analytical column (stainless steel, 25 cm * 4.6 mm i.d.) was packed with 5-*m particles of the reversed phase material and used for assays. Mobile phase containing 0.025 M sodium phosphate as aqueous phase and acetonitrile with 0.4% triethylamine as organic phase. The drugs were quantified at flow-rate of 1.2 ml/min, with ultraviolet detection at 260 nm. The minimum detectable quantities in assays were 100 ng/ml for SM and 75 ng/ml for TM. The method is well suited to routine application and adequate sensitivity with precision.}, Keywords = {Trimethoprim, Sulphamethoxazole, Co-trimoxazole, HPLC}, volume = {4}, Number = {2}, pages = {75-78}, publisher = {Pasteur Institute of Iran}, title_fa = {}, abstract_fa ={}, keywords_fa = {}, url = {http://ibj.pasteur.ac.ir/article-1-825-en.html}, eprint = {http://ibj.pasteur.ac.ir/article-1-825-en.pdf}, journal = {Iranian Biomedical Journal}, issn = {1028-852X}, eissn = {2008-823X}, year = {2000} }