@article{ author = {Shahrokhi, Nader and Bouzari, Saeid and Jafari, Anis}, title = {Priming Hepatitis B Surface (HBsAg)- and Core Antigen (HBcAg)-Specific Immune Responses by Chimeric, HBcAg with a HBsAg ‘a’ Determinant}, abstract ={We developed an immunogen to stimulate multivalent immunity against hepatitis B surface antigen (HBsAg) and hepatitis B core antigens (HBcAg). Immune responses specific for both HBsAg and HBcAg play an important role in controlling the infection. HBsAg-specific antibodies mediate elimination of virions at an early stage of infection and prevent the spread of virus. The immunogen was constructed by inserting the immunodominant, antibody-binding ‘a’ determinant (aa 111-149) of HBsAg (with or without a poly-glycine (PG) linker) into the e2 epitope of HBcAg. Only the constructs in which the HBsAg ‘a’ determinant was inserted into HBcAg, flanked by PG linkers, expressed a chimeric protein in human embryonic kidney cells with HBsAg and HBcAg antigenicity. Both glycosylated and non-glycosylated forms of the chimeric protein were immunoprecipitated from cell lysate. Intramuscular DNA vaccination of mice with plasmids expressing chimeric HBcAg primed antibody responses against well-defined serologically-defined determinants of both, native HBcAg, and native HBsAg. In addition, CD8 + T cell responses against HBcAg epitopes were primed by this chimeric HBV antigen. The e2 sequence of HBcAg can thus be used to present heterologous epitopes without loss of immunogenicity of the HBcAg protein.}, Keywords = {HBV vaccine, Hybrid protein, Poly-glycine (PG) linker}, volume = {10}, Number = {2}, pages = {61-68}, publisher = {Pasteur Institute of Iran}, title_fa = {القاء پاسخ ایمنی اختصاصی علیه آنتی‌ژن‌های سطحی و core ویروس هپاتیت B به وسیله پروتئین هیبرید آنتی‌ژن core و شاخص آنتی‌ژنیک a آنتی‌ژن s}, abstract_fa ={}, keywords_fa = {}, url = {http://ibj.pasteur.ac.ir/article-1-351-en.html}, eprint = {http://ibj.pasteur.ac.ir/article-1-351-en.pdf}, journal = {Iranian Biomedical Journal}, issn = {1028-852X}, eissn = {2008-823X}, year = {2006} } @article{ author = {Parvizi, Parviz and Ready, Paul D.}, title = {Molecular Investigation of the Population Differentiation of Phlebotomus papatasi, Important Vector of L. major, in Different Habitats and Regions of Iran}, abstract ={Phlebotomus papatasi is an important vector of L. major in Iran. P. papatasi was collected from peridomestic animal shelters, inside and around the houses and also the nearby burrows of the gerbil reservoir hosts, Rhombomys opimus, in several provinces in Iran. Mitochondrial Cytochrome b (Cyt b) of sandflies, which is a maternally-inherited gene marker, was used to see if there is any "isolation by distance" over a large geographical scale among Iranian provinces. The analyses were based on the last 717 bp of the Cyt b gene followed by 20 bp of intergenic spacer and the transfer tRNA ser (TCN) gene, i.e. the 737 bp fragment (without primers) amplified with the primers CB1-SE and CB-R06. Cyt b Long fragment sequences were obtained from 149 out of 177 specimens of P. papatasi and 49 haplotypes were identified. Based on the Cyt b Long fragment examined, P. papatasi showed only recent divergence in Iran, because the genetic distances between haplotypes were small. However, some evidence for isolation by distance was found. First, all the haplotypes from Iran did not belong to a single network, whereas most from Golestan province (Iran) did belong to a single network. Second, there were some abundant haplotypes that were found only in one province.}, Keywords = {Mitochondrial cytochrome b (Cyt b), Iranian sandflies, Phlebotomus papatasi, Isolation by distance}, volume = {10}, Number = {2}, pages = {69-77}, publisher = {Pasteur Institute of Iran}, title_fa = {یافته‌های مولکولی از تغییرات جمعیتی پشه خاکی فلبوتوموس پاپاتاسی ناقل اصلی لیشمانیا میجر از مناطق مختلف و زیستگاه‌های متفاوت ایران}, abstract_fa ={}, keywords_fa = {}, url = {http://ibj.pasteur.ac.ir/article-1-352-en.html}, eprint = {http://ibj.pasteur.ac.ir/article-1-352-en.pdf}, journal = {Iranian Biomedical Journal}, issn = {1028-852X}, eissn = {2008-823X}, year = {2006} } @article{ author = {GharibNaseri, Mohammad Kazem and Heidari, Akbar}, title = {Bronchodilatory Activity of Vitis vinifera Leaf Hydroalcoholic Extract in Rat}, abstract ={Several reports have shown the various effects of grape (Vitis vinifera) seed extract such as antioxidant, hypotensive, hypolipidemic and vasodilatory effects. The aim of present study was to investigate the effect of grape leaf hydroalcoholic extract on isolated rat tracheal contractions induced by KCl and acetylcholine. The trachea was removed from male adult Sprague-Dawley rat and placed in an organ bath containing Krebs-Henseleit solution and contractions were recorded isometrically. The results demonstrate that the grape leaf extract at 0.5, 1, 2, 4 and 8 mg/ml significantly reduces the tracheal contractions induced by KCl (60 mM) dose-dependently (P<0.0001). Acetylcholine (55 µM)induced tracheal contractions were also attenuated at the same concentration of the extract (P<0.0001). The grape leaf extract induced relaxation in the KCl-induced contraction in trachea was unaffected neither by nitric oxide (NO) synthase inhibitor (L-NAME, 100 µM) nor by betaadrenoceptor antagonist (propranolol 1 µM). Our results suggest that the bronchodilatory effect of grape leaf extract is mediated via the voltage dependent calcium channels. Furthermore, the betaadrenergic and NO are not involved.}, Keywords = {Grape leaf, Bronchodilatation, Rat}, volume = {10}, Number = {2}, pages = {79-83}, publisher = {Pasteur Institute of Iran}, title_fa = {اثر شل‌کنندگی عصاره آبی الکل برگ انگور در موش صحرایی}, abstract_fa ={}, keywords_fa = {}, url = {http://ibj.pasteur.ac.ir/article-1-353-en.html}, eprint = {http://ibj.pasteur.ac.ir/article-1-353-en.pdf}, journal = {Iranian Biomedical Journal}, issn = {1028-852X}, eissn = {2008-823X}, year = {2006} } @article{ author = {Farshad, Shohreh and Alborzi, Abdolvahab and Japoni, Aziz and Hayati, Masoumeh and Saberfirouzi, Mehdi and BagheriLankarani, Kamran and Taghavi, AliReza and Nasiri, Jalil and Rafatpour, Noreddi}, title = {Discrimination of Dominant H. pylori Strains Isolated from Patients with Different Gastroduodenal Pathologies by Protein Profiling}, abstract ={It is not clear what factors determine divergent outcomes of infections caused by H. pylori . In the present study, the protein profiles of different strains of H. pylori, isolated from three groups of patients with ulcerative disease, non-ulcerative gastritis and cancer disease, were analyzed using 1DSDS-PAGE. The patterns of different H. pylori strains were highly divergent. About 30.76% (7 bands) of the 26 observed protein bands were common in all strains isolated from 3 groups of the patients. While the similarity for the strains inside each group were 75% (15 from 20), 76.47% (13 from 17) and 78.57% (11 from 14) for cancerous, ulcerative and nonulcerative group, respectively. Some of the observed bands were significantly specific for each group. Therefore, we speculated that some H. pylori strains might be more associated with a specific disease than others, giving the clustering of some, but not all, strains within each disease group. In conclusion, this study showed that protein profile can be a characteristic in discrimination of dominant strains in different gastric clinical status. Specific and dominant proteins of different strains isolated from three groups of patients under study were candidates for further exploration for laboratory tests, which analyze disease-specific H. pylori strains, and for diagnosis of the different diseases and outcomes associated with this widespread bacterium.}, Keywords = {Helicobacter pylori, SDS-PAGE, Protein profile}, volume = {10}, Number = {2}, pages = {85-91}, publisher = {Pasteur Institute of Iran}, title_fa = {افتراق سوش‌های غالب هلیکوباکتر پیلوری جدا شده از بیماران مبتلا به پاتولوژی‌های مختلف دستگاه گوارش با استفاده از سیستم‌های پروتئینی}, abstract_fa ={}, keywords_fa = {}, url = {http://ibj.pasteur.ac.ir/article-1-354-en.html}, eprint = {http://ibj.pasteur.ac.ir/article-1-354-en.pdf}, journal = {Iranian Biomedical Journal}, issn = {1028-852X}, eissn = {2008-823X}, year = {2006} } @article{ author = {Moshtaghie, Ali Asghar and Ani, Mohsen and Arabi, Mohammad Hossei}, title = {Spectroscopic Studies on Titanium Ion Binding to the Apolactoferrin}, abstract ={Titanium (Ti) is a relatively abundant element that has found growing applications in medical science and recently some of Ti compounds are introduced as anticancer drugs. In spite of very limited data which exist on the Ti metabolism, some proteins might be involved in the mechanism of action of Ti. Up to our knowledge, there is not any report in the literature concerning binding of Ti to apolactoferrin. Binding of apolactoferrin with Ti(IV)-citrate was studied by spectroflourimeterey and spectrophotometery techniques under physiological conditions. The spectroflourimeteric studies revealed a significant fluorescence quenching, that indicated binding of apolactoferrin with Ti(IV). The same reaction was monitored through spectrophotometry technique this represents a characteristic UV difference band at 267 nm, which is different from lac-Fe (III). Titration studies show that lactoferrin specifically binds two moles Ti(IV) as complex with citrate per mol protein. Spectroflourimeterey and spectrophotometery techniques indicated that Ti(IV) ions cause a reduction (13%-14%) in binding of Fe(III) to lactoferrin. In overall, we may come to this conclusion that this element might be involved in the iron metabolism.}, Keywords = {Titanium (Ti), Lactoferrin, Iron}, volume = {10}, Number = {2}, pages = {93-98}, publisher = {Pasteur Institute of Iran}, title_fa = {مطالعه اسپکتروسکپی اتصال یون تیتانیوم به آپولاکتوفرین روی برانش}, abstract_fa ={}, keywords_fa = {}, url = {http://ibj.pasteur.ac.ir/article-1-457-en.html}, eprint = {http://ibj.pasteur.ac.ir/article-1-457-en.pdf}, journal = {Iranian Biomedical Journal}, issn = {1028-852X}, eissn = {2008-823X}, year = {2006} } @article{ author = {Roghani, Mehrdad and Joghataie, Mohammad Taghi and Jalili, Mohammad Reza and Baluchnejadmojarad, Tourandokht}, title = {Time Course of Changes in Passive Avoidance and Y-maze Performance in Male Diabetic Rats}, abstract ={Diabetes mellitus is accompanied with disturbances in learning, memory, and cognitive skills in the human society and experimental animals. Therefore, this research study was conducted to evaluate time-dependent changes in passive avoidance and Y-maze performance in male diabetic rats. For this purpose, male Wistar rats were randomly divided into control and diabetic groups. For induction of diabetes, streptozotocin (STZ) was injected i.p. at a single dose of 60 mg/kg. For evaluation of learning and memory, initial latency (IL) and step-through latency (STL) were determined at the end of 1 st , 2 nd , and 3 rd months using passive avoidance and Y-maze tasks. It was found out that mean IL exhibits a significant increase only at the end of 2 nd (p<0.05) and 3 rd (p<0.01) months. In addition, STL significantly reduced at the end of 2 nd (p<0.05) and 3 rd months (p<0.01). Regarding Y-maze task, alternation score of the diabetic rats was lower than that of the control ones at the end of 1 st (p<0.05), 2 nd (p<0.01), and 3 rd (p<0.01) months as compared to time-matched control group. To conclude, at least one month is strictly required for development of behavioral disturbances in passive avoidance and Y-maze tasks in STZ-diabetic rats.}, Keywords = {Learning and memory, Cognition, Passive avoidance, Y-maze, Diabetic rat, Streptozotocin (STZ)}, volume = {10}, Number = {2}, pages = {99-104}, publisher = {Pasteur Institute of Iran}, title_fa = {دوره زمانی تغییرات در رفتارهای اجتنابی Y-maze در موش‌های صحرایی دیابتی نر}, abstract_fa ={}, keywords_fa = {}, url = {http://ibj.pasteur.ac.ir/article-1-355-en.html}, eprint = {http://ibj.pasteur.ac.ir/article-1-355-en.pdf}, journal = {Iranian Biomedical Journal}, issn = {1028-852X}, eissn = {2008-823X}, year = {2006} } @article{ author = {Kariminia, Amina and K.Ardestani, Sussan and Al-SadatArdestani, Nargess and Sepehry, Houri and Darabi, Haideh}, title = {Interferon Gamma Unresponsiveness Due to Down-Regulation of IFN-γR Expression in Experimental Cutaneous Leishmaniasis}, abstract ={It is now well documented that interferon gamma (IFN-γ) is the indispensable cytokine for inducing protective immunity against experimental and human cutaneous leishmaniaisis. The importance of IFN-γ receptor (IFN-γR) has also been studied. In the present study, we made attempts to find out whether L. major infection is able to alter the expression of IFN-γR in vivo. In addition, we studied the responsiveness to IFN-γ ex vivo. To do that, we assessed the expression of CD119 (IFN-γRα ) on CD45+ cells isolated from draining lymph nodes of infected and uninfected BALB/c and C57BL/6 mice by flow cytometry. The MFI (mean fluorescence intensities) of CD119 on uninfected BALB/c mice were 192.8 ± 18.4 but the CD119 MFI of infected BALB/c mice were remarkably decreased (107.9±40.8). CD119 MFI of uninfected and infected C57BL/6 mice were 276.2 ± 17.1 and 140.4±43.0 respectively Moreover, we measured the production of nitric oxide (NO) by these cells in the presence of IFN-γ in order to study the function of IFN-γR. NO production by draining lymph nodes cells of infected C57BL/6 mice in response to recombinant murine IFN-γ was significantly higher than the cells of infected BALB/c mice (37.5 ± 0.6 and 11.6 ± 0.5 µM respectively, p<0.05). Therefore, our results confirm the in vitro reports regarding the impairment of IFN-γ responsiveness due to Leishmania infection.}, Keywords = {interferon gamma receptor (IFNγ R ) , Leishmania major, mice}, volume = {10}, Number = {2}, pages = {105-109}, publisher = {Pasteur Institute of Iran}, title_fa = {عدم پاسخ‌دهی به اینترفرون گاما متعاقب کاهش عرضه گیرنده اینترفرون گاما در لیشمانیوز پوستی تجربی}, abstract_fa ={}, keywords_fa = {}, url = {http://ibj.pasteur.ac.ir/article-1-356-en.html}, eprint = {http://ibj.pasteur.ac.ir/article-1-356-en.pdf}, journal = {Iranian Biomedical Journal}, issn = {1028-852X}, eissn = {2008-823X}, year = {2006} } @article{ author = {Asadi, Malek Hossein and Mowla, Seyed Javad and NikPoor, Parvaneh}, title = {Gene Expression Profile of CatSper3 and CatSper4 during Postnatal Development of Mouse Testis}, abstract ={Channel activities, particularly those of calcium channels, have vital roles in the process of sperm maturation, motility and sperm-egg interaction. A group of the recently discovered ion channels associated with these processes is four novel channel-like proteins known as CatSper (cation channel sperm) gene family. CatSper1 and CatSper2 show sperm specific expression patterns. However, neither CatSper1 nor CatSper2 have been shown to function as cation channels when transfected into cells, singly or together. It has been suggested that the known CatSper proteins require additional subunits and/or interaction partners to function. Using in silico gene identification and prediction techniques, two further members of the CatSper family, CatSper3 and CatSper4, have been identified. It has been hypothesized that the CatSper proteins may form a functional tetrameric channel. Based on these findings, we evaluated the temporal pattern of CatSper3 and CatSper4 genes during mouse testis development to assess any potential co-incidence of their expression with CatSper1 and CatSper2. In the present study, a total of 55 male BALB/c mice were categorized into 11 age groups: 1 day, 1 week, 2 weeks, 16, 18, 20, 22, 24 and 26 days, 1 month as well as adult mice. Testes were removed from each mouse, total RNA was extracted and RT-PCR was performed with specific primers for CatSper3, CatSper4 and beta-2-microglobin (as an internal control). Our results revealed that CatSper3 and CatSper4 genes are expressed predominantly at 3 week of age. The obtained data demonstrate that: 1) The expression of CatSper3 and CatSper4 in mouse is developmentally regulated. 2) There is a direct correlation between the temporary expressions of these genes and the other two members of the family, CatSper1 and CatSper2.}, Keywords = {CatSper, Fertility, Sperm motility, Testis, Gene expression}, volume = {10}, Number = {2}, pages = {111-115}, publisher = {Pasteur Institute of Iran}, title_fa = {بررسی بیان ژن کاتسپر 3 و 4 در حین تکوین جنینی بیضه موش}, abstract_fa ={}, keywords_fa = {}, url = {http://ibj.pasteur.ac.ir/article-1-357-en.html}, eprint = {http://ibj.pasteur.ac.ir/article-1-357-en.pdf}, journal = {Iranian Biomedical Journal}, issn = {1028-852X}, eissn = {2008-823X}, year = {2006} }