eng
Pasteur Institute of Iran
Iranian Biomedical Journal
1028-852X
2008-823X
2017-11
21
6
347
348
article
Glucose-Sensitive Holographic (Bio)Sensors: Fundamentals and Applications
Marjan Heidari
1
Bahareh Dabirmanesh
2
Hossein Mahdavi
3
Khosro Khajeh
4
Department of Biochemistry, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
Department of Biochemistry, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
School of Chemistry, Faculty of Sciences, University of Tehran, Tehran, Iran
Department of Biochemistry, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
Nowadays sensors and especially biosensors play an important role in medical diagnosis and detection of food and environment contaminations. Biosensors’ facilities have been improved significantly by using technologies such as surface plasmon resonance, microfluidics, laser, and electrochemistry. These technologies are now available on chips in micro- and nano-scale and are capable of measuring the quantity of more than one analyte at once. One branch of these sensors is the holographic (bio)sensor. Reflection holographic sensors, which are optical devices fabricated based on Bragg’s law, can diffract light with specific wavelength as they absorb or release special ions or molecule depending on their polymer structure. Their action is reversible and rapid and by measuring the color change, it is possible to determine the concentrations in real-time. Reversibility let the operator use a sensor several times. There are many sensors designed for measuring ethanol, metal ions, enzymes activity, and glucose...
http://ibj.pasteur.ac.ir/article-1-2152-en.pdf
eng
Pasteur Institute of Iran
Iranian Biomedical Journal
1028-852X
2008-823X
2017-11
21
6
349
359
article
A review of Brucellosis in Iran: Epidemiology, Risk Factors, Diagnosis, Control, and Prevention
Maryam Golshani
1
Saeid Buozari
2
Department of Molecular Biology, Pasteur Institute of Iran, Tehran, Iran
Department of Molecular Biology, Pasteur Institute of Iran, Tehran, Iran
Brucellosis caused by species of Brucella is among the most prevalent zoonoses with the annual incidence of half a million cases globally. Most parts of Iran are endemic for brucellosis, and the annual incidence of the human and animal brucellosis is still high. At present, there is no safe and protective human vaccine against brucellosis, and the only preventive strategy is animal vaccination, which harbors significant disadvantages. Considering the identification of many immunogenic proteins in Brucella, several studies have recently been performed to evaluate the vaccine potency of such antigens as a new subunit vaccine candidate. This review represents an overview of brucellosis in Iran, including epidemiology, transmission routs, diagnosis, and treatment. Moreover, it mainly highlights the history of brucellosis control and prevention in Iran, including eradication programs, vast livestock vaccination programs, and subunit vaccine studies. It also discusses major problems that the country encounters with disease control. In recent years, Persian scientists have focused on evaluating the efficacy of best Brucella immunogens in vivo to introduce a new subunit vaccine. The results of some studies could demonstrate the vaccine potential of some immunogens
http://ibj.pasteur.ac.ir/article-1-2141-en.pdf
Brucellosis
Iran
Epidemiology
Transmission
Subunit vaccine
eng
Pasteur Institute of Iran
Iranian Biomedical Journal
1028-852X
2008-823X
2017-11
21
6
360
368
article
In vitro Study of SPIONs-C595 as Molecular Imaging Probe for Specific Breast Cancer (MCF-7) Cells Detection
Pegah Moradi Khaniabadi
1
Daryoush Shahbazi-Gahrouei
2
Amin Malik Shah Abdul Majid
3
Mohammad Suhaimi Jaafar
4
Bita Moradi Khaniabadi
5
Saghar Shahbazi-Gahrouei
6
School of Physics, Universiti Sains Malaysia11800, Pulau Penang, Malaysia
Department of Medical Physics, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
School of Pharmaceutical Sciences, Universiti Sains Malaysia, 11800 Penang, Malaysia;
School of Physics, Universiti Sains Malaysia11800, Pulau Penang, Malaysia
Child Growth and Development Research Center, Research Institute for Primordial Prevention of Non-communicable Disease, Isfahan University of Medical Sciences, Isfahan, Iran
School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
Background: Magnetic resonance imaging (MRI) plays an essential role in molecular imaging by delivering the contrast agent into targeted cancer cells. The aim of this study was to evaluate the C595 monoclonal antibody-conjugated superparamagnetic iron oxide nanoparticles (SPIONs-C595) for the detection of breast cancer cell (MCF-7). Methods: The conjugation of monoclonal antibody and nanoparticles was confirmed using X-ray diffraction, transmission electron microscopy, and photon correlation spectroscopy. The selectivity of the nanoprobe for breast cancer cells (MCF-7) was obtained by Prussian blue, atomic emission spectroscopy, and MRI relaxometry. Results: The in vitro MRI showed that T2 relaxation time will be reduced 76% when using T2-weighed magnetic resonance images compared to the control group (untreated cells) at the dose of 200 μg Fe/ml, as the optimum dose. In addition, the results showed the high uptake of nanoprobe into MCF-7 cancer cells. Conclusion: The SPIONs-C595 nanoprobe has potential for the detection of specific breast cancer.
http://ibj.pasteur.ac.ir/article-1-2075-en.pdf
Nanoparticles
Molecular Imaging
MCF-7 cells
Monoclonal antibody
eng
Pasteur Institute of Iran
Iranian Biomedical Journal
1028-852X
2008-823X
2017-11
21
6
369
379
article
Physically Targeted Intravenous Polyurethane Nanoparticles for Controlled Release of Atorvastatin Calcium
Behnaz Sadat Eftekhari
1
Akbar Karkhaneh
2
Ali Alizadeh
3
Biomedical Engineering Department, Amirkabir University of Technology, Tehran, Iran
Biomedical Engineering Department, Amirkabir University of Technology, Tehran, Iran
Nanotechnology Research Center, Sharif University of Technology, Tehran, Iran
Background: Intravenous drug delivery is an advantageous choice for rapid administration, immediate drug effect, and avoidance of first-pass metabolism in oral drug delivery. In this study, the synthesis, formulation, and characterization of atorvastatin-loaded polyurethane (PU) nanoparticles were investigated for intravenous route of administration. Method: First, PU was synthesized and characterized. Second, nanoparticles were prepared in four different ratios of drug to polymer through two different techniques, including emulsion-diffusion and single-emulsion. Finally, particle size and polydispersity index, shape and surface morphology, drug entrapment efficiency (EE), drug loading, and in vitro release were evaluated by dynamics light scattering, scanning electron microscopy, and UV visible spectroscopy, respectively. Results: Within two methods, the prepared nanoparticles had a spherical shape and a smooth surface with a diversity of size ranged from 174.04 nm to 277.24 nm in emulsion-diffusion and from 306.5 nm to 393.12 in the single-emulsion method. The highest EE was 84.76%, for (1:4) sample in the emulsion-diffusion method. It has also been shown that in vitro release of nanoparticles, using the emulsion-diffusion method, was sustained up to eight days by two mechanisms: drug diffusion and polymer relaxation. Conclusion: PU nanoparticles, that were prepared by the emulsion-diffusion method, could be used as effective carriers for the controlled drug delivery of poorly water soluble drugs such as atorvastatin calcium.
http://ibj.pasteur.ac.ir/article-1-2051-en.pdf
Drug delivery systems
Nanoparticles
Polyurethanes
Cardiovascular diseases
eng
Pasteur Institute of Iran
Iranian Biomedical Journal
1028-852X
2008-823X
2017-11
21
6
380
391
article
Equilibrium Isotherm, Kinetic Modeling, Optimization, and Characterization Studies of Cadmium Adsorption by Surface-Engineered Escherichia coli
Vida Tafakori
1
Reza Zadmard
2
Fatemeh Tabandeh
3
Mohammad Ali Amoozegar
4
Gholamreza Ahmadian
5
Department of Cell and Molecular Sciences, School of Biological Science, Kharazmi University, Tehran, Iran
Chemistry and Chemical Engineering Research Center of Iran, Tehran, Iran;
Department of Industrial and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran;
Extremophiles Laboratory, Department of Microbiology, School of Biology, College of Science, University of Tehran, Tehran, Iran
Department of Industrial and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran;
Background: Amongst the methods that remove heavy metals from environment, biosorption approaches have received increased attention because of their environmentally friendly and cost-effective feature, as well as their superior performances. Methods: In the present study, we investigated the ability of a surface-engineered Escherichia coli, carrying the cyanobacterial metallothionein on the cell surface, in the removal of Ca (II) from solution under different experimental conditions. The biosorption process was optimized using central composite design. In parallel, the kinetics of metal biosorption was studied, and the rate constants of different kinetic models were calculated. Results: Cadmium biosorption is followed by the second-order kinetics. Freundlich and Langmuir equations were used to analyze sorption data; characteristic parameters were determined for each adsorption isotherm. The biosorption process was optimized using the central composite design. The optimal cadmium sorption capacity (284.69 nmol/mg biomass) was obtained at 40°C (pH 8) and a biomass dosage of 10 mg. The influence of two elutants, EDTA and CaCl2, was also assessed on metal recovery. Approximately, 68.58% and 56.54% of the adsorbed cadmium were removed by EDTA and CaCl2 during desorption, respectively. The Fourier transform infrared spectrophotometer (FTIR) analysis indicated that carboxyl, amino, phosphoryl, thiol, and hydroxyl are the main chemical groups involved in the cadmium bioadsorption process. Conclusion: Results from this study implied that chemical adsorption on the heterogeneous surface of E. coli E and optimization of adsorption parameters provides a highly efficient bioadsorbent.
http://ibj.pasteur.ac.ir/article-1-2060-en.pdf
Adsorption
Kinetics
Response surface methodology
Fourier Transform Infrared Spectrophotometer
eng
Pasteur Institute of Iran
Iranian Biomedical Journal
1028-852X
2008-823X
2017-11
21
6
392
399
article
Evaluation of Serum Interleukin-21 and HLA-C1 Polymorphism in Pediatrician Hematopoietic Stem Cell Transplantation for Early Diagnosis of Acute Graft-Versus-Host Disease
Nasrin Sehati
1
Parviz Kokhaei
2
Ali Motevalizade Ardekani
3
Raziyeh Tootoonchian
4
Fatemeh Pak
5
Student Research Committee, Semnan University of Medical Sciences, Semnan, Iran
Cancer Research Center and Department of Immunology, Semnan University of Medical Sciences, Semnan, Iran
National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran
Student Research Committee, Semnan University of Medical Sciences, Semnan, Iran
Cancer Research Center and Department of Immunology, Semnan University of Medical Sciences, Semnan, Iran
Background: Allogenic hematopoietic stem cell transplantation (HSCT) is a strategy used for treatment of different malignant diseases. However, success of allo-HSCT can be hampered by graft-versus-host-disease (GVHD). Natural killer (NK) cells may play an important role in activating antigen presenting cells and subsequent activation of T cells. The main purpose of this study was the evaluation of IL-21, as a blood biomarker, for early detection of acute GVHD (aGVHD) in children after HSCT and also the study of human leukocytes antigen (HLA)-C1 polymorphism, as a targeting ligand for NK cells in these patients. Methods: Fifty one children receiving HSCT were studied. Blood samples were collected at -8, 7, and 14 days of transplantation. The -8-day samples were analyzed for HLA-C1 polymorphism by PCR-sequence-specific primer technique and pre-transplantation IL-21 assay. To study the serum levels of IL-21, two blood samples were collected on days +7 and +14 and analyzed by ELISA technique. Results: The results indicated that the incidence of aGVHD in pediatric is associated with a polymorphism of HLA-C1, as alleles HLA-C01:12 (P<0.001), HLA-C01:22 (P<0.004), and HLA-C01:67 (P<0.009). On the other hand, the serum levels of IL-21 in children with aGVHD were decreased after transplantation compared to before transplantation. The serum levels of the IL-21 at 14 days after transplantation had a significant correlation with the occurrence of aGVHD (P=0.05). Conclusion: Based on the findings of this study, there is a significant correlation between HLA-C1 polymorphisms and the serum levels of IL-21 with the incidence of aGVHD.
http://ibj.pasteur.ac.ir/article-1-2087-en.pdf
Hematopoietic stem cell transplantation
NK cells
IL-21
eng
Pasteur Institute of Iran
Iranian Biomedical Journal
1028-852X
2008-823X
2017-11
21
6
400
405
article
Cheminformatics-Based Anticoagulant Study of Traditionally Used Medicinal Plants
Mahdi Alikhani Pour
1
Soroush Sardari
2
Ali Eslamifar
3
Abid Azhar
4
Mohammad Rezvani
5
Milad Nazari
6
Drug Design and Bioinformatics Unit, Department of Medical Biotechnology, Biotechnology Research Center, Pasteur Institute of Iran, Tehran 13164, Iran
Drug Design and Bioinformatics Unit, Department of Medical Biotechnology, Biotechnology Research Center, Pasteur Institute of Iran, Tehran 13164, Iran
Department of Clinical Research, Pasteur Institute of Iran, Tehran 13164, Iran;
Dr. A. Q. Khan Institute of Biotechnology and Genetic Engineering (KIBGE), University of Karachi, Karachi 75270, Pakistan
Department of Agricultural Biotechnology, Payame Noor University, Tehran, Iran
Department of Clinical Research, Pasteur Institute of Iran, Tehran 13164, Iran
Background: Medicinal plants, as a complementary medicine, have been used to treat various diseases since ancient times. These plants have numerous beneficial applications and are the source of certain conventional drugs. In diseases such as stroke and ischemia, which are caused by several factors, abnormal coagulation is an important causative factor. Accordingly, novel and effective therapies such as herbal remedies should be practiced to prevent such lethal diseases. Methods: Using the available databases such as Google Scholar and PubMed, the previously reported anticoagulant compounds and plants possessing anticoagulant activity were identified and collected in two separate lists. Next, the fast and cost-effective cheminformatics methods incorporated in PubChem were applied to detect some compounds similar to reported anticoagulants. Subsequently, 15 native medical plants of Iran containing the potential anticoagulants were selected. The selected plants were purchased and chopped, and the potential compounds were extracted by ethanol. Then three concentrations of extracts (1, 10, and 100 µg per ml) were made. Finally, anticoagulant effect of the selected plants was evaluated by in vitro prothrombin time and activated partial thromboplastin time coagulation tests. Results: Among the 15 selected medicinal plants, three plants, including Terminalia bellirica (P=0.0019), Astragalus arbusculinus (P=0.0021), and Origanum vulgare (P=0.0014) showed a more promising anticoagulant effect in comparison to the control. Conclusion: The anticoagulant activity was identified for the first time in these three plants. Further in vivo study and mechanism of action assay are required to be performed on these three plants, which could be suitable candidates for use as natural anticoagulant medicines.
http://ibj.pasteur.ac.ir/article-1-2026-en.pdf
Cheminformatics
Cardiovascular diseases
Medicinal plants
eng
Pasteur Institute of Iran
Iranian Biomedical Journal
1028-852X
2008-823X
2017-11
21
6
406
410
article
Improvement of Thermal Stability of BCG Vaccine
Fatemeh Jahanbakhsh Sefidi
1
Homan Kaghazian
2
Gholam Ali Moradli
3
Seyed Mehdi Hassanzadeh
4
Department of Microbiology, College of Basic Science, Islamic Azad University, Saveh, Iran
Research and Production Complex, Pasteur Institute of Iran, Tehran, Iran
Department of Microbiology, College of Basic Science, Islamic Azad University, Saveh, Iran
Research and Production Complex, Pasteur Institute of Iran, Tehran, Iran
Background: Thermal stability (TS) is a part of the BCG vaccine characterization by which the consistency of process in BCG vaccine production could be confirmed. To enhance the TS of the vaccine, some prevalent stabilizers in different concentrations were added to the final formulation of BCG bulk prior to Freeze-drying process. We found a formulation more effective than the current stabilizer for retaining the higher viability of lyophilized BCG vaccine produced by Pasteur Institute of Iran. Methods: In the design of experiments using Taguchi method, lactose, trehalose, glucose, dextran, and monosodium glutamate were added to the final formulation of BCG bulk prior to freeze-drying process. Viability of the samples was determined by counting the colony forming unit. Results: Maximum signal-to-noise ratio equal to maximum TS and viability was obtained by adding lactose, dextran, and glutamate in defined concentrations. Conclusion: Adding the stabilizers had a significant impact on TS of BCG vaccine to meet the quality requirements.
http://ibj.pasteur.ac.ir/article-1-2081-en.pdf
BCG vaccine
Quality control
Stability
eng
Pasteur Institute of Iran
Iranian Biomedical Journal
1028-852X
2008-823X
2017-11
21
6
411
416
article
E6-Specific Detection and Typing of Human Papillomaviruses in Oral Cavity Specimens from Iranian Patients
Hadi Razavi Nikoo
1
Abdollah Ardebili
2
Mehrdad Ravanshad
3
Fatemeh Rezaei
4
Ali Teimoori
5
Sayyad Khanizadeh
6
Mohammad Hassan Pouriayevali
7
Mehdi Ajorloo
8
Laboratory Science Research Center, Golestan University of Medical Sciences, Gorgan, Iran
Laboratory Science Research Center, Golestan University of Medical Sciences, Gorgan, Iran
Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
Health Research Institute, Infectious and Tropical Diseases Reseach Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
Hepatitis Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran
Department of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran, Iran
Hepatitis Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran
Background: Detection and quantification of human Papillomavirus (HPV) genome in oral carcinoma play an important role in diagnosis, as well as implications for progression of disease. Methods: We evaluated tissues from 50 esopharyngeal cancers collected from different regions of Iran for HPV E6 using the two type-specific primers sets. E6 gene of HPV genotypes was amplified by specific primers. The sensitivity of PCR assay was analyzed and determined using HPV-DNA-containing plasmids. Real-time PCR was utilized to determine the prevalence and HPV viral load in patients with oral cavity squamous cell carcinoma. Results: Eighteen (36%) specimens were positive for HPV. Among the 18 positive specimens, 10 showed HPV-18 (55.55%), and 8 specimens were positive for HPV-11 (44.44%). Of the 18 infected specimens, 6 (33.32%) and 12 (66.65%) were identified as high-titer and low-titer viral load, respectively. Conclusions: The PCR-based assay, developed in the current study, could be used for HPV detection, quantification, and genotyping in epidemiological and clinical studies.
http://ibj.pasteur.ac.ir/article-1-2031-en.pdf
Real-time PCR
Genotyping
Iran