eng
Pasteur Institute of Iran
Iranian Biomedical Journal
1028-852X
2008-823X
2000-10
4
4
113
116
article
Differentiation of M. scrofulaceum from Related Mycobacterial Species by Double-Diffusion Technique
Azar D. Khosravi
Khosraviaz@yahoo.com
1
John L Stanford
2
Isolation and differentiation of various species of mycobacteria are predominantly based on conventional, cultural and biochemical tests. Among the non-tuberculous mycobacteria, these tests are unable to differentiate M. scrofulaceum from M. avium-intracellulare (MAI) because of their identical biochemical characteristics. For this reason, one hundred mycobacterial strains suspected to be M. scrofulaceum, which had identical preliminary cultural and biochemical tests, were investigated by immunodiffusion technique. The standard sonicated antigens of M. scrofulaceum (22, 159, 1281) were used to sensitize rabbits and to produce specific antisera. The method was performed in plastic petri dishes containing agar and sodium azide. Antiserum was placed in the central well of the agar plate and the homologous ultrasonicates were in the peripheral wells. The results were observed one week later and the mycobacterial strains were divided according to the number of produced precipitation lines. Four different groups were identified on the basis of identical reaction with three antisera (three groups were M. scrofulaceum). Group 1 produced identical precipitation lines with antisera 22 and 1281, group 2 with antisera 22, 159 and 1281 and group 3 with antiserum 1281. The present study showed that the technique was able to differentiate M. scrofulaceum from MAI and was also able to identify the bacterium as distinct species. Moreover, by using different specific antisera, it is possible to determine different serotypes of M. scrofulaceum.
http://ibj.pasteur.ac.ir/article-1-590-en.pdf
Double diffusion
lymphadenitis
M. scrofulaceum
M avium complex
eng
Pasteur Institute of Iran
Iranian Biomedical Journal
1028-852X
2008-823X
2000-10
4
4
117
122
article
Serum Factors Induced the Nuclear Location of Annexin V in the Human Osteosarcoma Cell Line (MG-63)
javad Mohiti Ardakani
1
John Walker
2
Durdi Qujeq
3
Calcium-binding proteins play essential roles in the cell. One important class of calcium-binding proteins is the annexin family. This is a family of 13 proteins, which binds to phospholipids in a calcium-dependent manner. Osteosarcoma cell line (MG-63) is a transformed cell that has many characteristics of the differentiated cell, such as a considerable serum dependency in its growth rate. Using specific antibodies against each annexin and immunoflurescence microscopy, the location and relocation of the annexin V was determined by some serum factors. Serum starvation of MG-63 cells increases their doubling time from 24 hours to 4 days. Cells grown in serum contain high levels of annexin V in the cell nucleus whereas in the absence of serum results in loss of nuclear annexin V in about 75% of the cells. Refeeding cells with medium containing 10% serum restore annexin V to the nuclei within 5 hours. Charcoal-treated serum cannot allow annexin V to return to the nucleus. Inhibition of protein synthesis with cycloheximide does not prevent the serum-induced return of annexin V to the nuclei. However, treatment of cells with genistein at a concentration specific for inhibition of tyrosine kinases (200 M) inhibits the relocation of annexin V from cytoplasm to the nucleus. Thus, the cellular location of the annexin V depends on the growth state of the cells. It can be altered by the movement of this protein between the cytosol and the nucleus.
http://ibj.pasteur.ac.ir/article-1-591-en.pdf
Annexin V
Calcium
Osteosarcoma cells
eng
Pasteur Institute of Iran
Iranian Biomedical Journal
1028-852X
2008-823X
2000-10
4
4
123
128
article
Genomic Linkage Analysis of Iranian Clinical Isolates of Dermatophytes Fungi Using the RAPD-PCR
Hadi Jazayeri
1
Mohammad Hossein Motazedian
2
Masoud Emami
3
Dermatophytes are a group of keratinophilic fungi capable of invading keratinized tissues (skin, hair and nails). They cause dermatophytosis (commonly known as tinea or Ring worm) in human and animals. In this report, DNA similarities and genomic linkage of 40 dermatophytes strains was obtained from different universities, were studied by random amplified polymorphic DNA (RAPD–PCR) using 11 random primers. The similarity of Microsporum genus with two other genera was 13%, and the similarity of Trichophyton with Epidermophyton was 20.8%. These results provide the basis for the rapid identification of dermatophytes at the genetic level, in additions to the existing laboratory methods.
http://ibj.pasteur.ac.ir/article-1-592-en.pdf
Genomic linkage
Dermatophytes
RAPD-PCR
eng
Pasteur Institute of Iran
Iranian Biomedical Journal
1028-852X
2008-823X
2000-10
4
4
129
131
article
Immunogenicity of BHK-Rabies Vaccine in Cattle
Alireza Zavareh
zavarei@institute.pasteur.ac.ir
1
In this study, the immunogenicity of a rabies vaccine that was used in one of the Isfahan cattle farms is reported. This vaccine was produced by replication of the Pasteur virus (PV) strain on BHK-21 monolayer cell culture. The virus suspension was inactivated by ß-propiolactone treatment and the harvested pool showed sterility and safety. Thus, the obtained vaccine was tested on mice and a satisfactory immune response was observed. Thereafter, the vaccine was tested on 34 cattle divided into 2 groups. Each group was received a different dose of the vaccine intramuscularly. Sera were taken 2 months after rabies vaccination and were analyzed by the rapid fluorescence focus inhibition test (RFFIT) to evaluate the titer of the rabies-neutralizing antibody. Both groups under study showed a sufficient seroconversion.
http://ibj.pasteur.ac.ir/article-1-593-en.pdf
Rabies
Anti-rabies vaccine
BHK-21 cell
Cattle immunization