مجله بیومدیکال ایران IBJ Basic Sciences http://ibj.pasteur.ac.ir 1 admin 1028-852X 2008-823X - 10.66224/ibj - 8888 - en jalali 1391 7 1 gregorian 2012 10 1 16 4 online 1 fulltext

en اثر مهار بیان ژن EFG1 برکاهش بیان ژن ALS3 در مخمر Candida albicans توسط تکنولوژیRNAi Related Fields Related Fields مقاله کامل Full Length/Original Article Background: The most important virulence factor which plays a central role in Candida albicans pathogenesis is the ability of this yeast to alternate between unicellular yeast and filamentous hyphal forms. Efg1 protein is thought to be the main positive regulating transcription factor, which is responsible for regulating hyphal-specific gene expression under most conditions. ALS3 is one of the Efg1-associated genes encoding a multi-functional adhesive polypeptide, which mediates adherence to diverse host substrates. In this study, the EFG1 gene was knocked down by using synthetic siRNA in C. albicans and the regulation in ALS3 as one of the Efg1-dependent genes was investigated. Method: The 19-nucleotide siRNA was designed based on cDNA sequence of EFG1 gene in C. albicans. Transfection was performed using modified- plyethylen glycol/LiAc method. To quantify the level of EFG1 and the hyphal-specific ALS3 gene expression, the cognate EFG1 and ALS3 mRNA were measured in C. albicans by quantitative real-time RT-PCR. Results: Fluorescent microscopy pictures indicated that transfection was performed successfully. Also, according to relative expression software tool, expression of EFG1 gene was decreased significantly with 500 nM siRNA as well as 1 &micro;M siRNA (P<0.05). However, more significant down-regulations were observed in the expression of ALS3 in both concentrations of 500 nM and 1 &micro;M siRNA (P<0.05). Conclusion: In conclusion, we demonstrated the down-regulation of ALS3 gene as a consequent of applying EFG1-specific siRNA in C. albicans. This may lead us to design anti-fungal-specific agents in order to face with C. albicans-associated infections. Candida albicans, ALS3, RNAi, EFG1 172 178 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-366&slc_lang=en&sid=1 Maryam Moazeni مریم موذنی No Mohammad Reza Khorramizadeh محمدرضا خرمی زاده No Ladan Teimoori-Toolabi لادن تیموری تولابی No Fatemeh Noorbakhsh فاطمه نوربخش No Ali Akbar Fallahi علی اکبر فلاحی No Sassan Rezaie ساسان رضایی srezaie@tums.ac.ir Yes

en تاثیر پلی مورفیسم T677C ژن متیلن تتراهیدروفولات ردوکتاز و برهمکنش آن با عفونت هلیکوباکترپیلوری بر خطر ابتلا به سرطان معده Related Fields Related Fields مقاله کامل Full Length/Original Article Background: Attempts for early detection of gastric cancer have recently focused on host&#39;s genetic susceptibility factors and gene-environment interactions. We have, herein, studied the association of MTHFR C677T single nucleotide polymorphism (SNP) and its interaction with Helicobacter pylori infection, smoking, age and gender on the risk of gastric cancer among an Iranian population. Methods: Gastric cancer patients (n = 450) and cancer-free controls (n = 780) were studied for serum H. pylori-specific IgG antibodies by ELISA and MTHFR C677T polymorphism (SNP) by PCR-RFLP. Demographic and life style data were collected through patient interviews. Unconditional logistic regression model estimated odds ratio (OR) and the corresponding 95% confidence intervals (CI). Results: The interactions of MTHFR genotype with H. pylori infection (P = 0.03), age (P = 0.049) and gender (P = 0.007) were statistically significant. Accordingly, MTHFR C677T carriers who were also positive for H. pylori infection exhibited 80% (OR = 1.8, 95% CI = 1.0-2.9) significant excess risk of non-cardia gastric cancer. Furthermore, subjects over the age of 50 or female subjects carrying MTHFR C677T SNP showed 40 (OR = 1.4, 95% CI = 1.0-2.0) and 100 (OR = 2.0, 95% CI = 1.2-3.2) percent increased risk of gastric cancer, respectively. Conclusion: Therefore, MTHFR C677T SNP seems to increase the risk of gastric cancer and the effect is significantly inflated by interactions with H. pylori infection, age and gender. Helicobacter pylori, Smoking, Gender identity, Age group, Methylenetetrahydrofolate reductase 179 184 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-369&slc_lang=en&sid=1 Samaneh Saberi سمانه صابری No Kazem Zendehdel کاظم زنده دل No Sahar Jahangiri سحر جهانگیری No Yeganeh Talebkhan یگانه طالب خان No Afshin Abdirad افشین عبدی راد No Nazanin Mohajerani نازنین مهاجرانی No Maryam Bababeik مریم بابابیک No Najmeh Karami نجمه کریمی No Maryam Esmaili مریم اسمعیلی No Akbar Oghalaie اکبر عقلایی No Parisa Hassanpour پریسا حسن پور No Neda Amini ندا امینی No Mohammad Ali Mohagheghi محمدعلی محققی No Mahmoud Eshagh Hossieni محمود اسحاق حسینی No Marjan Mohammadi مرجان محمدی marjan.mohammadi2010@gmail.com Yes

en مطالعه مقایسه‌ای ویژگی‌های ایمنی شناختی و ساختاری دو کاندید واکسن نوترکیب علیه نوروتوکسین بوتولینوم تیپ E Related Fields Related Fields مقاله کامل Full Length/Original Article Background: Recently, botulinum neurotoxin (BoNT)-derived recombinant proteins have been suggested as potential botulism vaccines. Here, with concentrating on BoNT type E (BoNT/E), we studied two of these binding domain-based recombinant proteins: a multivalent chimer protein, which is composed of BoNT serotypes A, B and E binding subdomains, and a monovalent recombinant protein, which contains 93 amino acid residues from recombinant C-terminal heavy chain of BoNT/E (rBoNT/E-HCC). Both proteins have an identical region (48 aa) that contains one of the most important BoNT/E epitopes (YLTHMRD sequence). Methods: The recombinant protein efficiency in antibody production, their structural differences, and their BoNT/E-epitope location were compared by using ELISA, circular dichroism, computational modeling, and hydrophobicity predictions. Results: Immunological studies indicated that the antibody yield against rBoNT/E-HCC was higher than chimer protein. Cross ELISA confirmed that the antibodies against the chimer protein recognized rBoNT/E-HCC more efficiently. However, both antibody groups (anti-chimer and anti-rBoNT/E-HCC antibodies) were able to recognize other proteins. Structural studies with circular dichroism showed that chimer proteins have slightly more secondary structures than rBoNT/E-HCC. Conclusion: The immunological results suggested that the above-mentioned identical region in rBoNT/E-HCC is more exposed. Circular dichroism, computational protein modeling and hydrophobicity predictions indicated a more exposed location for the identical region in rBoNT/E-HCC than the chimer protein, which is strongly in agreement with immunological results. Botulinum neurotoxin type E (BoNT/E), Cross ELISA, circular dichroism, Computational modeling, recombinant vaccine-candidates 185 192 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-372&slc_lang=en&sid=1 Mosayeb Rostamian مصیب رستمیان No Seyed Jafar Mousavy سید جعفر موسوی jmosavi@ihu.ac.ir Yes Firouz Ebrahimi فیروز ابراهیمی No Seyyed Abolghasem Ghadami سید ابوالقاسم قدمی No Nader Sheibani نادر شیبانی No Mohammad Ebrahim Minaei محمد ابراهیم مینایی No Mohammad Ali Arefpour Torabi محمد علی عارف پور ترابی No

en نقش داربست های نانوفایبری زیست تخریب پذیر توام با سلول های بنیادی فولیکول مو در مهندسی بافت Related Fields Related Fields مقاله کامل Full Length/Original Article Background: The aim of this study was to fabricate the poly caprolactone (PCL) aligned nanofiber scaffold and to evaluate the survival, adhesion, proliferation, and differentiation of rat hair follicle stem cells (HFSC) in the graft material using electrospun PCL nanofiber scaffold for tissue engineering applications. Methods: The bulge region of rat whisker was isolated and cultured in DMEM: nutrient mixture F-12 supplemented with epidermal growth factor. The morphological and biological features of cultured bulge cells were observed by light microscopy using immunocytochemistry methods. Electrospinning was used for production of PCL nanofiber scaffolds. Scanning electron microscopy (SEM), 3-(4, 5-di-methylthiazol- 2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay, and histology analysis were used to investigate the cell morphology, viability, attachment and infiltration of the HFSC on the PCL nanofiber scaffolds. Results: The results of the MTT assay showed cell viability and cell proliferation of the HFSC on PCL nanofiber scaffolds. SEM microscopy images indicated that HFSC are attached, proliferated and spread on PCL nanofiber scaffolds. Also, immunocytochemical analysis showed cell infiltration and cell differentiation on the scaffolds. Conclusion: The results of this study reveal that PCL nanofiber scaffolds are suitable for cell culture, proliferation, differentiation and attachment. Furthermore, HFSC are attached and proliferated on PCL nanofiber scaffolds. Nanofiber, Electrospinning, Stem cells, Tissue engineering 193 201 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-373&slc_lang=en&sid=1 Leila Beigom Hejazian لیلا بیگم حجازیان No Banafshe Esmaeilzade بنفشه اسمعیل زاده No Fatima Moghanni Ghoroghi فاطمه مغنی قرقی No Fatemeh Moradi فاطمه مرادی No Marzieh Beigom Hejazian مرضیه بیگم حجازیان No Anahita Aslani آناهیتا اصلانی No Mehrdad Bakhtiari مهرداد بختیاری No Masoud Soleimani مسعود سلیمانی No Maliheh Nobakht ملیحه نوبخت m-nobakht@tums.ac.ir Yes

en کاهش ضایعه موضعی مغز با مهار آنزیم مبدل آنژیوتانسین بعد از ایسکمی در موش صحرائی با فشار نرمال Related Fields Related Fields مقاله کامل Full Length/Original Article Background: Central renin angiotensin system has an important role on the cerebral microcirculation and metabolism. Our previous work showed that inhibition of angiotensin converting enzyme (ACE) activity prior to induction of ischemia protected the brain from severe ischemia/reperfusion (I/R) injuries. This study evaluated the impacts of post-ischemic inhibition of ACE, enalapril, on brain infarction in normotensive rats. Methods: Rats were anesthetized with chloral hydrate (400 mg/kg). Focal cerebral ischemia was induced by 60-min intraluminal occlusion of right middle cerebral artery (MCA). Intraperitoneal injection of enalapril (0.03 or 0.1 mg/kg) was done after MCA reopening (reperfusion). Neurological deficit score (NDS) was evaluated after 24 h and the animals randomly assigned for the assessments of infarction, absolute brain water content (ABWC) and index of brain edema. Results: Severe impaired motor functions (NDS = 2.78 &plusmn; 0.28), massive infarction (cortex = 214 &plusmn; 19 mm3, striatum = 86 &plusmn; 5 mm3) and edema (ABWC = 83.1 &plusmn; 0.46%) were observed in non-treated ischemic rats. Non-hypotensive dose of enalapril (0.03 mg/kg) significantly reduced NDS (1.5 &plusmn; 0.22), infarction (cortex = 102 &plusmn; 16 mm3, striatum = 38 &plusmn; 5 mm3) and edema (ABWC = 80.9 &plusmn; 0.81%). Enalapril at dose of 0.1 mg/kg significantly lowered arterial pressure could not improve NDS (2.0 &plusmn; 0.45) and reduce infarction (cortex = 166 &plusmn; 26 mm3, striatum = 71 &plusmn; 11 mm3). Conclusion: Post-ischemic ACE inhibition in the normotensive rats without affecting arterial pressure protects the brain from reperfusion injuries however, this beneficial action is masked by hypotension. Angiotensin converting enzyme (ACE) inhibitors, Enalapril, Brain edema, Cerebral infarction 202 208 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-370&slc_lang=en&sid=1 Hamdollah Panahpour حمداله پناهپور No Gholam Abbas Dehghani غلامعباس دهقانی dehghang@sums.ac.ir Yes

en پروماستیگوت های لیشمانیا ماژور غنی شده با پروتئین های شوک حرارتی در موش BALB/c ایجاد کننده پاسخ ایمنی Th2 Related Fields Related Fields مقاله کامل Full Length/Original Article Background: Heat shock proteins (HSP) are highly conserved molecules with many immunological functions. They are highly immunogenic with important role in cancer immunotherapy and in vaccine development against infectious diseases. As adjuvant, HSP can augment the immunogenicity of weak antigens and can stimulate antigen presenting cells. Although vaccines have been successful for many infectious diseases, progress in leishmaniasis has not been achieved. In this report, the protective effect of HSP-enriched soluble leishmania antigen (SLA) was determined. Methods: BALB/c mice were immunized 3&times; with HSP-enriched SLA and SLA alone and 10 days after final boost. They were infected with 106 stationary phase promastigote of Leishmania major and immunological responses were followed until nine weeks. Results: No significant differences were observed in lymphocyte proliferation, footpad swelling, parasite burden, nitric oxide or IL-12 cytokine between HSP-enriched or SLA groups. Although the levels of IFN-&gamma;, IL-4, TGF-&beta;, IgG1 and IgG2b were increased in both groups, IFN-&gamma; was significantly higher in SLA group and IgG2a in HSP-enriched SLA. Conclusion: These results indicate that HSP direct the immune system towards Th2 pattern and does not have protective role in L. major infection. Leishmaniasis, Heat shock proteins (HSP), Adjuvant 209 217 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-367&slc_lang=en&sid=1 Marzieh Holakuyee مرضیه هولاکویی No Mehdi Mahdavi مهدی مهدوی No Zuhair Mohammad Hassan زهیر محمد حسن No Mohsen Abolhassani محسن ابوالحسنی mabolhassani@yahoo.com Yes

en نقش پلیمرفیسم Ile3434Thr ژن XRCC7 در احتمال سرطان تیروئید تمایز یافته در یک جمعیت ایرانی Related Fields Related Fields مقاله کامل Full Length/Original Article Background: The aim of this study was to understand any association between differentiated thyroid carcinoma (DTC) and Ile3434Thr XRCC7 gene polymorphism (GenBank accession number: rs7830743). DTC is the most prevalent thyroid neoplasm, which includes papillary and follicular cell carcinoma. XRCC7 gene encodes a protein that functions in non-homologous end joining DNA repair pathway. Non-synonymous polymorphisms in this gene may alter DNA repair capacity of the cell and change the risk of developing cancers. Methods: DTC patients (n = 173) and cancer free individuals (n = 204) were enrolled in a case-control study. The Ile3434Thr polymorphic alleles were discriminated by using amplification refractory mutation system-PCR method. The frequencies of this single nucleotide polymorphism in case and control groups were compared. Also, risk ratio for developing DTC in dichotomized genotypes was estimated by multivariate logistic regression analysis. Results: Dichotomized genotypes into those with and without the 3434Thr allele showed that this allele was associated with DTC (OR [odd ratio]: 1.89, 95% confidence interval (CI) = 1.29-2.79, P<0.001). Also, TC genotype was significantly associated with increased risk of DTC (OR: 2.42, 95% CI = 1.55-3.81, P = 0.0001) in individuals carrying this genotype. Conclusion: Allele 3434Thr in XRCC7 gene might be associated with differentiated thyroid cancer risk. Further studies with larger samples are needed to verify these initial findings. DNA repair enzymes, Thyroid neoplasms, Genetic polymorphism 218 222 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-374&slc_lang=en&sid=1 Maryam Rahimi مریم رحیمی No Shima Fayaz شیما فیاض No Armaghan Fard-Esfahani ارمغان فرد اصفهانی No Mohammad Hossein Modarressi محمد حسین مدرسی No Seyed Mohammad Akrami سید محمد اکرمی No Pezhman Fard-Esfahani پژمان فرد اصفهانی fard-esfahani@pasteur.ac.ir Yes

en یک موتاسیون جدید در ژن (APTX) Apratoxinدر یک بیمار ایرانی مبتلا به بیماری آتاکسی زودرس چشمی همراه با آپراکسی نوع 1 (AOA1) Related Fields Related Fields مقاله کوتاه Short Communication <p>Background: Ataxia with oculomotor apraxia type 1 (AOA1) shows early onset with autosomal recessive inheritance and is caused by a mutation in the aprataxin (APTX) gene encoding for the APTX protein. Methods: In this study, a 7-year-old girl born of a first-cousin consanguineous marriage was described with early-onset progressive ataxia and AOA, with increased cholesterol concentration and decreased albumin concentration in serum. PCR and direct DNA sequencing was performed after DNA extraction. Results: Sequencing analysis revealed a novel homozygous deletion in c.643 and A>T single nucleotide polymorphism in c.641 in exon 6 of the APTX gene [ENST00000379825]. Conclusion: It seems that this region of exon 6 is probably a hot spot however, no deletions have been reported in exon 6 yet.</p> Ataxia oculomotor apraxia 1 (AOA1), aprataxin (APTX), Iranian 223 225 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-368&slc_lang=en&sid=1 Nayereh Nouri نیره نوری No Narges Nouri نرگس نوری No Omid Aryani امید آریانی No Behnam Kamalidehghan بهنام کمالی دهقان No Maryam Sedghi مریم صدقی No Massoud Houshmand مسعود هوشمند massoudh@nigeb.ac.ir Yes

en (نامه به سردبیر) تکه تکه شدن DNA با آپوپتوز HepG2 سلول در Zerumbone ناشی نشده همراه Related Fields Related Fields نامه به سردبیر Letter to the Editor <p>Zerumbone is a cytotoxic compound isolated from the herbal plant, Zingiber zerumbet Smith, which exhibits antitumor activity [1-2], anti-inflammatory effects and possesses anti-proliferative potentials in a variety of cell lines [3-4]. DNA fragmentation indicates an early event of apoptosis leading to cell death due to the absence of new cellular proteins synthesizing for cell survival. Previous studies indicated that the cleavage of double-stranded DNA in apoptotic DNA degradation occurs via the activation of endogenous Ca2+/Mg2+-dependent endonuclease that specifically cleaves between nucleosomes to produce DNA fragments that are multiples of ~180 base pairs [5].In order to investigate DNA fragmentation, we treated HepG2 cells with zerumbone (IC50: 3.45 &plusmn; 0.026 &micro;g/mL) in both dose-dependent (2, 4, 6 and 8 &micro;g/mL) and time-dependent manner (4, 8, 12, 16, 24, 48 and 72 h). The assay was performed using the Suicide Track&trade; DNA Ladder Isolation Kit (Calbiochem, CA, USA), according to the manufacturer&#39;s instructions. DNA was analyzed using 1.5% agarose gel electrophoresis, observed under UV illumination and visualized using a gel documentation system (UVP Biospectrum HR410, USA). To furthur confirm the induction of apoptosis, the protein of zerumbone-induced HepG2 cells using Western-blotting indicated a low and high expression of Bcl2 and Bax proteins, respectively.In conclusion, these results indicate that no DNA fragmentation in the human hepatocellular liver carcinoma (HepG2) cells was observed even in the presence of caspase-3 during apoptosis. Therefore, we hypothesize that not all compounds necessairly indicate fragmentation of condensed chromatin into several discrete mass in cell lines as in vitro condition.</p> 226 226 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-371&slc_lang=en&sid=1 Behnam Kamalidehghan بهنام کمالی دهقان Behnam@um.edu.my Yes Fatemeh Ahmadipour فاطمه احمدی پور No Mohamed Ibrahim Noordin محمد ابراهیم نورالدین No