Iranian Biomedical Journal

en Radiosensitivity and Repair Kinetics of Gamma-Irradiated Leukocytes from Sporadic Prostate Cancer Patients and Healthy Individuals Assessed by Alkaline Comet Assay Radiosensitivity and Repair Kinetics of Gamma-Irradiated Leukocytes from Sporadic Prostate Cancer Patients and Healthy Individuals Assessed by Alkaline Comet Assay Related Fields Related Fields مقاله کامل Full Length/Original Article Background: Impaired DNA repair mechanism is one of the main causes of tumor genesis. Study of intrinsic radiosensitivity of cancer patients in a non-target tissue (e.g. peripheral blood) might show the extent of DNA repair deficiency of cells in affected individuals and might be used a predictor of cancer predisposition. Methods: Initial radiation-induced DNA damage (ratio of Tail DNA/Head DNA), dose-response curves and kinetics of DNA repair in leukocytes from healthy volunteers and prostate cancer patients were assessed using alkaline comet assay after exposure to 60Co gamma rays. Results: Results showed that higher levels of baseline and gamma rays induced DNA damage in leukocytes of prostate cancer cases than in controls. A similar dose response was obtained for both groups. After a repair time of 24 h following in vitro irradiation, samples from the healthy individuals showed no residual DNA damage in their leukocytes, whereas prostate cancer patients revealed more than 20%. Although similar initial radiosensitivity was observed for both groups, the repair kinetics of radiation induced DNA damage of leukocytes from prostate cancer cases and healthy subjects were statistically different. Conclusion: These results support the hypothesis that men affected by prostate cancer may have a constitutional genomic instability. Leukocytes, DNA damage , Radiosensitivity, Prostate cancer, Comet assay 67 75 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-172&slc_lang=en&sid=1 Maryam Shahidi مریم شهیدی No Hossein Mozdarani حسین مزدرانی mozdarah@modares.ac.ir Yes Wolfgang-Ulrich Mueller Wolfgang-Ulrich Mueller No

en Expression of ROR1 in Patients with Renal Cancer-A Potential Diagnostic Marker Expression of ROR1 in Patients with Renal Cancer-A Potential Diagnostic Marker Related Fields Related Fields مقاله کامل Full Length/Original Article Background: The ectopic expression of receptor tyrosine kinase Ror1 has been reported in patients with hematological malignancies such as chronic lymphocytic leukemia and acute lymphoblastic leukemia. Here we report, for the first time, expression of ROR1 gene in both tumor tissues and peripheral blood mononuclear cells (PBMC) from patients with renal cancer (RC). Methods: In the current study, the expression of ROR1 gene was semi-quantitatively measured in PBMC and tumor tissues from 16 RC patients as well as PBMC from 22 healthy individuals relative to the expression of the housekeeping gene phosphoglucomutase 1 by RT-PCR. Results: Our results showed that ROR1 was expressed at gene level in 81.3% of renal tumor tissues (13 out of 16) whereas it was expressed in 94% of PBMC from RC patients (15 out of 16). A weak expression of ROR1 was observed in PBMC of 4 out of 22 healthy individual. A significant expression of ROR1 was observed in PBMC from RC patients when compared to that in PBMC from normal healthy individuals (P<0.001). The expression of ROR1 in PBMC may reflect a shedding of tumor cells into blood stream. Conclusion: We conclude that detection of a high level of ROR1 expression in blood cells might assist in early detection of renal malignancies, providing taking into consideration the clinical symptoms of the disease. ROR1, Ectopic expression, Renal cancer 77 82 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-173&slc_lang=en&sid=1 Hodjattallah Rabbani حجت اله ربانی No Mahyar Ostadkarampour مهیار استاد کرم پور No Amir Hossein Danesh Manesh امیر حسین دانش منش No Abbas Basiri عباس بصیری No Mahmood Jeddi-Tehrani محمود جدی تهرانی No Flora Forouzesh فلورا فروزش f_foruzesh@yahoo.com Yes

en Localization of Herpes Simplex Virus Type 1 DNA in Latently Infected BALB/c Mice Neurons Using in situ Polymerase Chain Reaction Localization of Herpes Simplex Virus Type 1 DNA in Latently Infected BALB/c Mice Neurons Using in situ Polymerase Chain Reaction Related Fields Related Fields مقاله کامل Full Length/Original Article Background: Herpes simplex virus type-1 (HSV-1) establishes a lifelong latent infection in neurons following primary infection. The existence of latent HSV-1 DNA in the trigeminal ganglia of infected BALB/c mice was examined using a direct in situ PCR technique, based on Digoxigenin-11-dUTP detection system with anti-digoxigenin-peroxidase and 3,3'-diaminobenzidine (DAB) substrate. Methods: Eight-week-old male BALB/c mice were inoculated via the eye by 104 plaque forming unit of wild type Iranian isolates of HSV-1. After establishment of latency, trigeminal ganglia were removed and examined using in situ PCR to detect HSV-1 genome. Finally, the results of in situ PCR were verified by a two-round PCR method, using amplification cocktail of in situ reaction, as a template for a conventional gel base PCR. Results and Conclusion: The results suggest that a direct in situ PCR method using a peroxidase and DAB detection system is a useful means for detection of latent HSV-1 DNA in the latently infected ganglia. Herpes simplex virus-1, Latency, In situ PCR, two-round PCR, Trigeminal ganglia 83 88 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-174&slc_lang=en&sid=1 Behzad Khansarinejad بهزاد خوانساری نژاد No Hoorieh Soleimanjahi حوریه سلیمانجاهی soleim_h@modares.ac.ir Yes Amir Ghaemi امیر قائمی No Taki Tiraihi تقی طریحی No Shahram Pour Beiranvand شهرام پور بیران وند No

en Association between ABCB1-T1236C Polymorphism and Drug-Resistant Epilepsy in Iranian Female Patients Association between ABCB1-T1236C Polymorphism and Drug-Resistant Epilepsy in Iranian Female Patients Related Fields Related Fields مقاله کامل Full Length/Original Article Background: One third of epileptic patients are resistant to several anti-epileptic drugs (AED). P-glycoprotein (P-gp) is an efflux transporter encoded by ATP-binding cassette subfamily B member 1 (ABCB1) gene that excludes drugs from the cells and plays a significant role in AEDs resistance. Over-expression of P-gp could be a result of polymorphisms in ABCB1 gene. We studied the association of T129C and T1236C single-nucleotide polymorphisms (SNP) of ABCB1 gene with drug-resistant epilepsy in Iranian epileptics. Methods: DNA samples were obtained from 200 healthy controls and 332 epileptic patients, of whom 200 were drug responsive and 132 drug resistant. The frequencies of the genotypes of the two SNP were determined by polymerase chain reaction followed by restriction fragment length polymorphism. Results: No significant association was found between T129C and T1236C genotypes and drug-resistant epilepsy neither in adults nor in children. However, the risk of drug resistance was higher in female patients with 1236CC (P = 0.02) or CT (P = 0.008) genotype than in those with TT genotype. The risk of drug resistance was also higher in patients with symptomatic epilepsies with 1236CC (P = 0.02) or CT (P = 0.004) genotype than in those with TT genotype. The risk of drug resistance was lower in patients with idiopathic epilepsies with 129TT genotype (P = 0.001) than in those with CT genotype. Conclusion: Our results indicate that T1236C polymorphism is associated with drug resistance in Iranian female epileptic patients. Replication studies with large sample sizes are needed to confirm our results. ATP-binding cassette subfamily B member 1 (ABCB1), Drug-resistant epilepsy, Single nucleotide polymorphism 89 96 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-175&slc_lang=en&sid=1 Mehri Maleki مهری ملکی No Mohammad Sayyah محمد سیاح sayyahm2@pasteur.ac.ir Yes Fatemeh Kamgarpour فاطمه کامگرپور No Morteza Karimipoor مرتضی کریمی پور No Aida Arab آیدا عرب No Anahita Rajabi آناهیتا رجبی No Kourosh Gharagozli کوروش قره گوزلی No Ahmad Reza Shamshiri احمد رضا شمشیری No Esmaeil Shahsavand Ananloo اسماعیل شاهسوند انانلو No

en Molecular Cloning, Expression and Enzymatic Assay of Pteridine Reductase 1 from Iranian Lizard Leishmania Molecular Cloning, Expression and Enzymatic Assay of Pteridine Reductase 1 from Iranian Lizard Leishmania Related Fields Related Fields مقاله کامل Full Length/Original Article Background: Currently, there are no effective vaccines against leishmaniasis, and treatment using pentavalent antimonial drugs is occasionally effective and often toxic for patients. The PTR1 enzyme, which causes antifolate drug resistance in Leishmania parasites encoded by gene pteridine reductase 1 (ptr1). Since Leishmania lacks pteridine and folate metabolism, it cannot synthesize the pteridine moiety from guanine triphosphate. Therefore, it must produce pteridine using PTR1, an essential part of the salvage pathway that reduces oxidized pteridines. Thus, PTR1 is a good drug-target candidate for anti-Leishmania chemotherapy. The aim of this study was the cloning, expression, and enzymatic assay of the ptr1 gene from Iranian lizard Leishmania as a model for further studies on Leishmania. Methods: Promastigote DNA was extracted from the Iranian lizard Leishmania, and the ptr1 gene was amplified using specific primers. The PCR product was cloned, transformed into Escherichia coli strain JM109, and expressed. The recombinant protein (PTR1 enzyme) was then purified and assayed. Results: ptr1 gene was successfully amplified and cloned into expression vector. Recombinant protein (PTR1 enzyme) was purified using affinity chromatography and confirmed by Western-blot and dot blot using anti-Leishmania major PTR1 antibody and anti-T7 tag monoclonal antibody, respectively. The enzymatic assay was confirmed as PTR1 witch performed using 6-biopterin as a substrate and nicotinamide adenine dinucleotide phosphate as a coenzyme. Conclusion: Iranian lizard Leishmania ptr1 was expressed and enzymatic assay was performed successfully. Pteridine reductase 1 (PTR1), Leishmania, Gene expression 97 102 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-176&slc_lang=en&sid=1 Bahram Kazemi بهرام کاظمی bahram_14@yahoo.com Yes Farideh Tohidi فریده توحیدی No Mojgan Bandehpour مژگان بنده پور No Fatemeh Yarian فاطمه یاریان No

en Effects of Leukemia Inhibitory Factor on gp130 Expression and Rate of Metaphase II Development during in vitro Effects of Leukemia Inhibitory Factor on gp130 Expression and Rate of Metaphase II Development during in vitro maturation of mouse oocyte Related Fields Related Fields مقاله کامل Full Length/Original Article Background: Leukemia inhibitory factor (LIF) is a 45-56 kDa glycoprotein that has an important role in proliferation and embryo implantation. Its effect on oocyte maturation and how to exert the function remained to be elucidated. Methods: Immature mice superovulated with human menopausal gonadotropin and germinal vesicle (GV) oocytes were obtained from ovary 48 hours after. GV oocytes were cultured in tissue culture medium 199 with 0, 100, 500 and 1,000 U/ml LIF. Cumulus expansion and in vitro maturation (IVM) rate were assessed after culture. For reverse transcriptase PCR, total RNA from GV and metaphase II (MII) oocytes were extracted by Trizol reagent. The quantity and quality of RNA were determined by spectrophotometry and electrophoresis, respectively. Reverse transcription was performed by Super Script III reverse transcriptase with 1 µg of total RNA followed by DNase I treatment and heat inactivation. Expression of gp130 was determined by RT-PCR. Results: Our results showed that cumulus expansion was improved with 1,000 U/ml in culture medium compared to others. GV breakdown and MII rate in groups with LIF were higher than control group and were dose dependant. In 1,000 U/ml, LIF rate of MII was significantly higher than control group (P<0.05). Our results also showed that gp130 is expressed neither in GV nor in MII oocytes during IVM of mouse oocytes. Conclusion: gp130 is expressed in human oocyte but not in mouse. Our results suggest that in mouse, LIF could affect the oocyte via another receptor or via cumulus cells however, further studies are warranted. Leukemia inhibitory factor (LIF), Oocytes, In vitro maturation (IVM) 103 107 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-178&slc_lang=en&sid=1 Amaneh Mohammadi Roushandeh آمنه محمدی روشنده mohammadir@tbzmed.ac.ir Yes Hassan Haji Hosseinlou حسن حاجی حسینلو No Behrouz Niknafs بهروز نیک نفس No Raheleh Halabian راحله حلبیان No Ahmad Mehdipour احمد مهدی پور No Mehryar Habibi Roudkenar مهریار حبیبی رودکنار No

en The Correlation between the Endometrial Integrins and Osteopontin Expression with Pinopodes Development in Ovariectomized Mice in Response to Exogenous Steroids Hormones The Correlation between the Endometrial Integrins and Osteopontin Expression with Pinopodes Development in Ovariectomized Mice in Response to Exogenous Steroids Hormones Related Fields Related Fields مقاله کامل Full Length/Original Article Background: The ovariectomized animals are good models to evaluate the effect of different steroid hormone treatments on implantation events and the pattern of integrin expression. Therefore, this study was performed to compare the expression of integrins and osteopontin (OPN) in correlation with pinopode development in ovariectomized mice endometrium which was subjected to steroid hormones. Methods: Ovariectomized mice were subjected to estrogen, progesterone and estrogen-progesterone hormones. Their uterine horns were evaluated for integrin expression by immunohistochemistry and real-time RT-PCR and for pinopode development by transmission and scanning electron microscopic studies. Results: No immunostaining for integrin and OPN molecules were detected in the endometrium of non-ovariectomized mice except in metestrus phase. The α4 and β1 integrin genes were expressed in all phases of estrous cycle. In ovariectomized mice, no reaction was detected in the endometrium of control, sham and estrogen-treated groups, but in both progesterone-treated groups, all examined genes were expressed. There was not any correlation between pinopodes and integrin expression in ovariectomized mice. Conclusion: The progesterone is more effective on endometrial integrin expression than estrogen and differences in the expression pattern of integrins reflect their important and different roles in embryo implantation. The pinopodes may have minor effect in mice implantation or have some delay in their expressions in ovariectomized mice which were subjected to exogenous hormones. Endometrium, Estrogen, Integrin, Ovariectomized mice, Osteopontin (OPN) 109 119 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-179&slc_lang=en&sid=1 Fatemeh Peyghambari فاطمه پیغمبری No Mojdeh Salehnia مژده صالح نیا salehnim@modares.ac.ir Yes Mehdi Forouzandeh Moghadam مهدی فروزنده مقدم No Mojtaba Rezazadeh Valujerdi مجتبی رضازاده ولوجردی No Ebrahim Hajizadeh ابراهیم حاجی زاده No

en Eucalyptus globulus (Eucalyptus) Treatment of Candidiasis in Normal and Diabetic Rats Eucalyptus globulus (Eucalyptus) Treatment of Candidiasis in Normal and Diabetic Rats Related Fields Related Fields مقاله کامل Full Length/Original Article Background: The leaves of Eucalyptus globulus (eucalyptus) are used for treatment of diabetes mellitus in traditional medicine. The aim of this study was to evaluate the effects of eucalyptus in treatment of established systemic infection with Candida albicans in normal and streptozotocin-induced diabetic rats. Methods: Sixty normoglycemic male Wistar rats, weighing 200-250 g, were selected and randomly divided into six groups (n= 10): normal control, control + C. albicans, control + eucalyptus + C. albicans, diabetic control, diabetic + C. albicans, diabetic + eucalyptus + C. albicans. Diabetes was induced after a single intraperitoneal injection of streptozotocin (60 mg/kg body weight) and eucalyptus was added to the diet (62.5 g/kg) and drinking water (2.5 g/L) of treated animals for 4 weeks. The concerned groups were inoculated with C. albicans 15 days after diabetes induction. At the end of one month experiment, fasted rats were killed by cervical decapitation. Blood was collected from neck vein for estimation of glucose. C. albicans concentrations were estimated in liver and kidneys using serial dilution culture of tissue homogenates. Results: Eucalyptus administration significantly improved the hyperglycemia, polydipsia, polyphagia, and it also compensated weight loss of diabetic rats (P<0.05). Moreover, eucalyptus caused a significant reduction in C. albicans concentration in liver and kidney homogenates (P<0.01). Conclusion: The results revealed that eucalyptus improves Candidia infection in normal and diabetic rats that in some ways validates the traditional use of this plant in treatment of diabetic patients. Diabetes mellitus, Eucalyptus globulus, Candida albicans 121 126 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-180&slc_lang=en&sid=1 Mohammad Bokaeian محمد بکائیان bokaeian.m@gmail.com Yes Alireza Nakhaee علیرضا نخعی No Bita Moodi بیتا مودی No Hossein Ali Khazaei حسینعلی خزاعی No