Iranian Biomedical Journal
مجله بیومدیکال ایران
IBJ
Basic Sciences
http://ibj.pasteur.ac.ir
1
admin
1028-852X
2008-823X
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10.61186/ibj
-
8888
-
en
jalali
1392
4
1
gregorian
2013
7
1
17
3
online
1
fulltext
en
اثر پیوند سلولهای بنیادی مزانشیمی مغز استخوان ترانسفکت شده با p75 siRNA بر بهبود ضایعه نخاعی در مدل موش صحرایی
Transplanting P75-Suppressed Bone Marrow Stromal Cells Promotes Functional Behavior in a Rat Model of Spinal Cord Injury
Related Fields
Related Fields
مقاله کامل
Full Length/Original Article
Background: Bone marrow stromal cells (BMSC) have been successfully employed for movement deficit recovery in spinal cord injury (SCI) rat models. One of the unsettled problems in cell transplantation is the relative high proportion of cell death, specifically after neural differentiation. According to our previous studies, p75 receptor, known as the death receptor, is only expressed in BMSC in a time window of 6-12 hours following neural induction. Moreover, we have recently reported a decreased level of apoptosis in p75-suppressed BMSC in vitro. Therefore, our objective in this research was to explore the functional effects of transplanting p75:siRNA expressing BMSC in SCI rats. Methods: Laminectomy was performed at L1 vertebra level to expose spinal cord for contusion using weight-drop method. PBS-treated SCI rats (group one) were used as negative controls, in which cavitations were observed 10 weeks after SCI. pRNA-U6.1/Hygro- (group two, as a mock) and pRNA-U6.1/Hygro-p75 shRNA- (group three) transfected BMSC were labeled with a fluorescent dye, CM-DiI, and grafted into the lesion site 7 days after surgery. The Basso-Beattie-Bresnehan locomotor rating scale was performed weekly for 10 weeks. Results: There was a significant difference (P≤0.05) between all groups of treated rats regarding functional recovery. Specifically, the discrepancy among p75 siRNA and mock-transfected BMSC was statistically significant. P75 siRNA BMSC also revealed a higher level of in vivo survival compared to the mock BMSC. Conclusion: Our data suggest that genetically modified BMSC that express p75:siRNA could be a more suitable source of cells for treatment of SCI.
Spinal cord injury (SCI), Apoptosis, Bone marrow stromal cells (BMSC)
140
145
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-481&slc_lang=en&sid=1
Houri
Edalat
حوری
عدالت
100319475328460056294
100319475328460056294
No
Zahra
Hajebrahimi
زهرا
حاج ابراهیمی
100319475328460056295
100319475328460056295
No
Vahid
Pirhajati
وحید
پیرحاجاتی
100319475328460056296
100319475328460056296
No
Mansoureh
Movahedin
منصوره
موحدین
100319475328460056297
100319475328460056297
No
Mahmoud
Tavallaei
محمود
تولایی
100319475328460056298
100319475328460056298
No
Mohammad-Reza
Soroush
محمدرضا
سروش
100319475328460056299
100319475328460056299
No
Seyed Javad
Mowla
سید جواد
مولی
sjmowla@modares.ac.ir
100319475328460056300
100319475328460056300
Yes