en مقاله کامل Full Length/Original Article Prostate-specific antigen (PSA) was purified to homogeneity from human seminal plasma by ion-exchange chromatography on a CM-Sephadex C-50 and by gel filtration on a Sephacryl S-200 column. A single 33-kDa protein band appeared in SDS-PAGE. High pressure liquid chromatography (HPLC) of the purified protein produced a single peak, while isoelectric focusing demonstrated the presence of five different isoforms of this protein. The immunoreactivity of the purified PSA was checked by Western blotting. This simple two-step method can be used for a large-scale preparation of the purified PSA for the clinical tests and also for further investigative studies on the biological properties of this protein. Prostate-specific antigen, purification, semen, chromatography 89 91 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-406&slc_lang=en&sid=1 Eskandar Kamali Sarvestani اسکندر کمالی سروستانی No Abdolaziz Khezri عبدالعزیز خضری No Mohamoud Vessal محمود وصال No Abbas Ghaderi عباس قادری immunol@summs.ac.ir Yes