Iranian Biomedical Journal
مجله بیومدیکال ایران
IBJ
Basic Sciences
http://ibj.pasteur.ac.ir
1
admin
1028-852X
2008-823X
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10.61186/ibj
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8888
-
en
jalali
1378
10
1
gregorian
2000
1
1
4
1
online
1
fulltext
en
Expression of Hepatitis B Virus Core Antigen in Native and Fusion Forms in E. coli
Expression of Hepatitis B Virus Core Antigen in Native and Fusion Forms in E. coli
مقاله کامل
Full Length/Original Article
DNA coding for the core antigen from hepatitis B Virus (HBcAg) was amplified, cloned and propagated in E. coli. The core protein was expressed in E. coli and the product was readily detected by Western blot. This protein can be used as a diagnostic material in serum screening tests. To increase the level of expression of this antigen in bacteria, two plasmids were constructed in which the gene coding for N-terminal part of the human IL-2 has been fused to 5¢ -terminus of the core antigen gene under control of the tryptophan promoter. Although the expression level of core antigen from hepatitis B Virus in E. coli was increased in fusion forms, but the size of fusion partner could affect the antigenicity, particle formation and assembly of the core antigen. The native and the two fusion forms of the core antigens from hepatitis B Virus were evaluated as a diagnostic material in serum screening.
Hepatitis B core antigen, HBcAg, HBV, Expression
37
43
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-400&slc_lang=en&sid=1
Mohammad
Azizi
محمد
عزیزی
100319475328460057943
100319475328460057943
Yes
Alexis
Musacchio
Alexis
Musacchio
100319475328460057944
100319475328460057944
No
Ornaldo
Pardo
Ornaldo
Pardo
100319475328460057945
100319475328460057945
No
Nelvis
Figueroa
Nelvis
Figueroa
100319475328460057946
100319475328460057946
No
Verena
Muzio
Verena
Muzio
100319475328460057947
100319475328460057947
No