en بررسی پایلوت راهکارهائی جهت ارزیابی بهتردو بیومارکرخون محیطی در مبتلایان به سرطان کولورکتال توسط روش Real-time RT-PCR Related Fields Related Fields مقاله کامل Full Length/Original Article Background: Efficient screening for detection of colorectal cancer (CRC) at earlier stages reduces its mortality. The purpose of this study was to investigate expression of carcinoembryonic antigen (CEA) and human telomerase reverse transcriptase (hTERT) mRNA in peripheral blood of CRC patients and to present strategies for early detection screen test. Methods: Twenty seven patients in non-metastatic stage and 27 healthy individuals were studied. Expression of CEA, hTERT mRNA and 18srRNA (18s subunit of ribosomal RNA, as reference gene) were determined based on real-time RT-PCR on 3 &micro;g of total RNA from blood in 3 separate vials (1 &micro;g per vial). Results: Positive expression rate of CEA mRNA (78%) and hTERT mRNA (81%) were higher in patient group (P<0.001). These rates were meaningfully higher than the results of individual vials containing only 1 &micro;g of total RNA. Difference between Ct values of markers with 18srRNA (&Delta;Ct) was higher in healthy group than patient one. Therefore, a &Delta;Ct cut-off value was determined for distinguishing between true- and false-positive results. Concurrent expression of both markers was found in 67% of the patients, which was higher than healthy cases (11%). Combination of concurrent marker expression with cut-off point strategy increased specificity to 100%. Conclusion: These results showed that concurrent evaluation of marker expression and performing the test on 3 &micro;g of samples in 3 separate vials may increase specificity and sensitivity of real-time RT-PCR for early detection of non-metastatic CRC. However, more investigations with larger numbers of samples are needed to verify these results. Carcinoembryonic antigen, Biomarker, Colorectal cancer 15 21 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-378&slc_lang=en&sid=1 Reza Yadegarazari رضا یادگارآذری No Taghi Hassanzadeh تقی حسن زاده No Amir Majlesi امیر مجلسی No Amir Keshvari امیر کشوری No Alireza Monsef Esfahani علیرضا منصف اصفهانی No Amirsasan Tootoonchi امیر ساسان توتونچی No Nooshin Shabab نوشین شباب No Massoud Saidijam مسعود سعیدی جم sjam110@yahoo.com Yes