Iranian Biomedical Journal
مجله بیومدیکال ایران
IBJ
Basic Sciences
http://ibj.pasteur.ac.ir
1
admin
1028-852X
2008-823X
-
10.61186/ibj
-
8888
-
en
jalali
1391
4
1
gregorian
2012
7
1
16
3
online
1
fulltext
en
واکسن DNA علیه Escherichia coli O157:H7
A DNA Vaccine against Escherichia coli O157:H7
Related Fields
Related Fields
مقاله کامل
Full Length/Original Article
Background: Infection with Escherichia coli O157:H7 rarely leads to bloody diarrhea and causes hemolytic uremic syndrome with renal failure that can be deadly dangerous. Intimin, translocated Intimin receptor (Tir), and enterohemorrhagic E. coli (EHEC) secreted protein A (EspA) proteins are the virulence factors expressed by locus of enterocyte effacement locus of EHEC. This bacterium needs EspA as a conduit for Tir delivery into the host cell and the surface arrayed Intimin, which docks the bacterium to the translocated Tir. Methods: Here we used triplet synthetic gene (eit) which was designed from three genes: espA coding EspA 120 lacking 36 amino acids from the N-terminal of the protein, eae coding Intimin constructed of 282 amino acids from the C-terminal and tir coding Tir 103, residues 258-361 which interacts with Intimin. The multimeric gene was cloned in two eukaryotic vectors pAAV-multiple cloning site-green fluorescent protein and pCI-neo. The pAAV was used for gene expression assay in cell line 293T and pCI-neo-EIT (EspA, Intimin, Tir) was used as DNA vaccine in mice. Test groups were injected intramuscularly with pCI-neo-EIT four times and mice control group was injected under the same conditions with PBS or pCI-neo vector. Results: The titration of serums showed that BALB/c mice were successfully immunized with DNA vaccine compared to control groups and also they were protected against challenges of live oral using E. coli O157:H7. Conclusion: The results suggest that the DNA vaccine could induce protective immunity either alone or in combination with purified antigens to reduce EHEC infection.
DNA vaccines, Escherichia coli O157:H7, Virulence factors
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http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-365&slc_lang=en&sid=1
Kowsar
Shariati Mehr
کوثر
شریعتی مهر
100319475328460056468
100319475328460056468
No
Seyed Latif
Mousavi
سید لطیف
موسوی
slmousavi@shahed.ac.ir
100319475328460056469
100319475328460056469
Yes
Iraj
Rasooli
ایرج
رسولی
100319475328460056470
100319475328460056470
No
Jafar
Amani
جعفر
امانی
100319475328460056471
100319475328460056471
No
Masoumeh
Rajabi
معصومه
رجبی
100319475328460056472
100319475328460056472
No