en ترمیم ضایعه نخاعی بوسیله پیوند همزمان نورون های حرکتی مشتق از سلول های بنیادی جنینی و سلول های پوشاننده پیاز بویایی Related Fields Related Fields مقاله کامل Full Length/Original Article Background: The failure of regeneration after spinal cord injury (SCI) has been attributed to axonal demyelination and neuronal death. Cellular replacement and white matter regeneration are both necessary for SCI repair. In this study, we evaluated the co-transplantation of olfactory ensheathing cells (OEC) and embryonic stem (ES) cell-derived motor neurons (ESMN) on contused SCI. Methods: OEC cultured from olfactory nerve rootlets and olfactory bulbs. ESMN was generated by exposing mouse ES cells to retinoic acid and sonic hedgehog. Thirty female rats were used to prepare SCI models in five groups. Control and medium-injected groups was subjected to induce lesion without cell transplantation. OEC or ESMN or both were transplanted into the site of the lesion in other groups. Results: The purity of OEC culture was 95%. Motor neuron progenitor markers (Olig2, Nkx6.1 and Pax6) and motor neuron markers (Isl1, Isl2 and Hb9) were expressed. Histological analysis showed that significantly more (P<0.001) spinal tissue was spared in OEC, ESMN and OEC+ ESMN groups but the OEC+ ESMN group had a significantly greater percentage of spared tissue and myelination than other groups (P< 0.05). The numbers of ESMN in co-transplanted group were significantly higher than ESMN group (P<0.05). A significant (P<0.05) recovery of hindlimb function was observed in rats in the transplanted groups. Conclusion: We found that the co-transplantation of ESMN and OEC into an injured spinal cord has a synergistic effect, promoting neural regeneration, ESMN survival and partial functional recovery. Spinal cord injury (SCI), Embryonic stem (ES) cell, Motor neuron, Olfactory ensheathing cell (OEC) 125 135 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-36&slc_lang=en&sid=1 Mohammad Salehi محمد صالحی No Parichehr Pasbakhsh پریچهر پاس بخش No Masoud Soleimani مسعود سلیمانی No Mehdi Abbasi مهدی عباسی No Gholamreza Hasanzadeh غلامرضا حسن زاده No Mohammad Hossein Modaresi محمدحسین مدرسی No Aligholi Sobhani علیقلی سبحانی sobhania@tums.ac.ir Yes