Iranian Biomedical Journal
مجله بیومدیکال ایران
IBJ
Basic Sciences
http://ibj.pasteur.ac.ir
1
admin
1028-852X
2008-823X
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10.61186/ibj
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8888
-
en
jalali
1391
3
1
gregorian
2012
6
1
16
2
online
1
fulltext
en
شناسایی بتائین به عنوان مهارکننده تولید نیتریک اکساید القاء شده توسط لیپو پلی ساکارید در سلولهای میکروگلیای ملتهب
Recognition of Betaine as an Inhibitor of Lipopolysaccharide-Induced Nitric Oxide Production in Activated Microglial Cells
Related Fields
Related Fields
مقاله کامل
Full Length/Original Article
<p>Background: Neuroinflammation, as a major outcome of microglia activation, is an important factor for progression of neurodegenerative disorders including Alzheimer's disease and Parkinson's disease. Microglial cells, as the first-line defense in the central nervous system, act as a source of neurotoxic factors such as nitric oxide (NO), a free radical which is involved in neuronal cell death. The aim of this study was to inhibit production of NO in activated microglial cells in order to decrease neurological damages that threat the central nervous system. Methods: An in vitro model of a newborn rat brain cell culture was used to examine the effect of betaine on the release of NO induced by lipopolysaccharide (LPS). Briefly, primary microglial cells were stimulated by LPS and after 2 minutes, they were treated by different concentrations of betaine. The production of NO was assessed by the Griess assay while cell viability was determined by the MTT assay. Results: Our investigations indicated that LPS-induced NO release was attenuated by betaine, suggesting that this compound might inhibit NO release. The effects of betaine on NO production in activated microglial cells after 24 h were "dose-dependent". It means that microglial cells which were treated with higher concentrations of betaine, released lowers amount of NO. Also our observations showed that betaine compound has no toxic effect on microglial cells. Conclusion: Betaine has an inhibitory effect on NO release in activated microglial cells and may be an effective therapeutic component to control neurological disorders.</p>
Betaine, Lipopolysaccharides (LPS), Nitric oxide (NO), Microglia
84
89
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-361&slc_lang=en&sid=1
Banafsheh
Amiraslani
بنفشه
امیراصلانی
100319475328460056518
100319475328460056518
No
Farzaneh
Sabouni
فرزانه
صابونی
sabouni@nigeb.ac.ir
100319475328460056519
100319475328460056519
Yes
Shahsanam
Abbasi
شاه صنم
عباسی
100319475328460056520
100319475328460056520
No
Habiballah
Nazem
حبیب الله
ناظم
100319475328460056521
100319475328460056521
No
Mohammadsadegh
Sabet
محمد صادق
ثابت
100319475328460056522
100319475328460056522
No