Iranian Biomedical Journal
مجله بیومدیکال ایران
IBJ
Basic Sciences
http://ibj.pasteur.ac.ir
1
admin
1028-852X
2008-823X
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10.61186/ibj
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8888
-
en
jalali
1388
4
1
gregorian
2009
7
1
13
3
online
1
fulltext
en
تاثیرژن Vhs ویروس هرپس سیمپلکس به عنوان یک ژن جدید خودکشی سلولی بر سلولهای توموری
The Effect of Herpes Simplex Virus Virion Host Shutoff Gene- a New Suicide Gene- on Tumor Cells
Related Fields
Related Fields
مقاله کوتاه
Short Communication
<p>Background: The herpes simplex virus (HSV) UL41 gene product, virion host shutoff (Vhs) protein, mediates the rapid degradation of both viral and cellular mRNA. This ability suggests that Vhs protein can be used as a suicide gene in cancer gene therapy applications. The recent reports have shown that the degradation of cellular mRNA during herpes simplex infection is selective. RNA containing AU-rich elements (ARE) in their 3’ untranslated ends are the targets for the Vhs protein. RNA that are not subject to Vhs protein-dependent degradation are up-regulated during HSV infection. ARE are frequently found in mRNA that encode proto-oncogenes, nuclear transcription factors, and cytokines. In many human cancers, the AU-rich stretch of proto-oncogenes and regulatory genes has impaired. Methods: To investigate whether Vhs protein might be useful for inhibition of tumor cell proliferation, a eukaryotic expression vector containing Vhs protein gene was constructed. Cell degradation and RNA content of HeLa and MRC-5 tumor cells after transfection with the constructed vector were studied. Results: The results showed a strong inhibitory activity in proliferation of transfected tumor cells and a sharp decrease in their RNA content. Conclusion: These data suggest that Vhs protein can be considered as a candidate for suicide cancer gene therapy.</p>
Herpes simplex virus (HSV), Virion host shut off (Vhs), Tumor, RNA degradation
185
189
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-31&slc_lang=en&sid=1
Hossei
Bannazadeh Baghi
حسین
بنازاده باقی
100319475328460056875
100319475328460056875
No
Taravat
Bamdad
طراوت
بامداد
bamdad_T@modares.ac.ir
100319475328460056876
100319475328460056876
Yes
Hoorieh
Soleimanjahi
حوریه
سلیمانجاهی
100319475328460056877
100319475328460056877
No