Iranian Biomedical Journal
مجله بیومدیکال ایران
IBJ
Basic Sciences
http://ibj.pasteur.ac.ir
1
admin
1028-852X
2008-823X
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10.61186/ibj
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8888
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en
jalali
1390
10
1
gregorian
2012
1
1
16
1
online
1
fulltext
en
بررسی سیتوژنتیک و جهش های FLT3 در بیماران لوسمی پرومیلوسیتیک حاد
Cytogenetic and FMS-Like Tyrosine Kinase 3 Mutation Analyses in Acute Promyelocytic Leukemia Patients
Related Fields
Related Fields
مقاله کامل
Full Length/Original Article
Background: The secondary genetic changes other than the promyelocytic leukemia-retinoic acid receptor (PML-RARA) fusion gene may contribute to the acute promyelocytic leukemogenesis. Chromosomal alterations and mutation of FLT3 (FMS-like tyrosine kinase 3) tyrosine kinase receptor are the frequent genetic alterations in acute myeloid leukemia. However, the prognostic significance of FLT3 mutations in acute promyelocytic leukemia (APL) is not firmly established. Methods: In this study, the chromosomal abnormalities were analyzed by bone marrow cytogenetic in 45 APL patients and FLT3 internal tandem duplications (ITD) screening by fragment length analysis and FLT3 D835 mutation by melting curve analysis were screened in 23 APL samples. Results: Cytogenetic study showed 14.3% trisomy 8 and 17.1% chromosomal abnormalities other than t(1517). About 13% of the patients had FLT3 ITD, and 26% had D835 point mutation. FLT3 ITD mutation was associated with higher white blood cell count at presentation and poor prognosis. Conclusion: The PML-RARA translocation alone may not be sufficient to induce leukemia. Therefore, we assume that FLT3 mutations and the other genetic and chromosomal alterations may cooperate with PML-RARA in the development of APL disease.
Chromosome aberration, FMS-like tyrosine kinase 3, Acute promyelocytic leukemia
10
17
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-353&slc_lang=en&sid=1
Marjan
Yaghmaie
مرجان
یغمایی
100319475328460056551
100319475328460056551
No
Kamran
Alimoghaddam
کامران
علی مقدم
100319475328460056552
100319475328460056552
No
Hossein
Mozdarani
حسین
مزدرانی
100319475328460056553
100319475328460056553
No
Ardeshir
Ghavamzadeh
اردشیر
قوام زاده
100319475328460056554
100319475328460056554
No
Marjan
Hajhashemi
مرجان
حاج قاسمی
100319475328460056555
100319475328460056555
No
Mozaffar
Aznab
مظفر
ازنب
100319475328460056556
100319475328460056556
No
Seyed H.
Ghaffari
سید حمداله
غفاری
shghaffari200@yahoo.com
100319475328460056557
100319475328460056557
Yes