en تمایز سلول های بنیادی مشتق شده از بافت چربی به سلول های شبه عصبی بیان کننده نوروتروفین ها تحت القاء سلژلین Related Fields Related Fields مقاله کامل Full Length/Original Article <font face="times new roman,times,serif"><font size="2"><span new="" roman="" style="FONT-FAMILY: " times=""><strong>Background: </strong></span><span new="" roman="" style="FONT-FAMILY: " times="">Adult stem cells (ASC) are undifferentiated cells found throughout the body. These cells are promising <span style="COLOR: black mso-themecolor: text1">tools for cell replacement therapy in neurodegenerative disease. Adipose tissue is the most abundant and accessible source of ASC. T</span></span></font></font><span new="" roman="" style="FONT-FAMILY: " times=""><font face="times new roman,times,serif" size="2">his</font><span class="hps"><font size="3"><font face="times new roman,times,serif" size="2"> study was conducted to</font><font face="Times New Roman">&nbsp;</font></font></span><font size="2">evaluat</font></span><font size="2"><span new="" roman="" style="FONT-FAMILY: " times="">e</span><span class="hps"><span new="" roman="" style="FONT-FAMILY: " times="">&nbsp;</span><span new="" roman="" style="FONT-FAMILY: " times="">effect of </span></span></font><span new="" roman="" style="FONT-FAMILY: " times=""><font size="2">selegiline</font><span class="hps"><font face="Times New Roman" size="3"><font size="2"> on</font> <font size="2">differentiation</font> <font size="2">of</font> </font></span></span><span new="" roman="" style="FONT-FAMILY: " times="">adipose-derived stem cells (ADSC) </span><span class="hps"><span new="" roman="" style="FONT-FAMILY: " times="">into functional neuron-like cells (NLC), </span><span new="" roman="" style="FONT-FAMILY: " times="">and also </span><span new="" roman="" style="FONT-FAMILY: " times="">level of the n</span></span><span class="st"><span new="" roman="" style="FONT-FAMILY: " times="">eurotrophin </span><span new="" roman="" style="FONT-FAMILY: " times="">expression in differentiated cells.<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span><font face="Times New Roman" size="3">&nbsp;</font></span></span></span><b><span new="" roman="" style="FONT-FAMILY: " times="">Methods: </span></b><span class="hps"><span new="" roman="" style="FONT-FAMILY: " times="">ADSC</span></span><span new="" roman="" style="FONT-FAMILY: " times=""> were transdifferentiated <font face="times new roman,times,serif" size="2">into NLC using selegiline where CD90, CD49d, CD31, CD106 and CD45 were used as markers for ADSC identification. Lipogenic and osteogenic differentiation of ADSC were used to characterize the ADSC. ADSC were treated with selegiline at different concentrations (from 10^-6 to 10^-11 mM) and time points (3, 6, 12, 24 and 48 h). Percentage of viable cells, nestin and neurofilament 68 (NF-68) immunoreactive cells were used as markers for differentiation. The optimal dose for neurotrophin expressions in differentiating cells was evaluated using r</font></span><span new="" roman="" style="FONT-FAMILY: " times=""><font face="times new roman,times,serif" size="2">everse transcriptase-</font><span style="COLOR: black mso-themecolor: text1"><font size="3"><font face="times new roman,times,serif" size="2">PCR.</font><font face="Times New Roman"> <font face="times new roman,times,serif" size="2">NLC function was evaluated by loading and unloading with FM1-43 dye. <b>Results: </b>ADSC were immunoreactive to CD90 (95.67 &plusmn; 2.26), CD49d (71.52 &plusmn; 6.64) and CD31 (0.6 &plusmn; 0.86), but no immunoreactivity was detected for CD106 and CD45. The results of neural differentiation</font> </font><font face="times new roman,times,serif" size="2">rative diseases using selegiline to induce ADSC differentiation to neuronal lineage.</font></font><font face="times new roman,times,serif" size="2">showed the highest percentage of nestin and NF-68 positive cells at 10^-9 mM concentration of selegiline (exposed for 24 h). The differentiated cells expressed synapsin and neurotrophin<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>genes<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>except b<em><span style="FONT-STYLE: normal mso-bidi-font-family: 'Times New Roman' mso-ascii-font-family: 'Times New Roman' mso-hansi-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-theme-font: major-bidi mso-bidi-theme-font: major-bidi">rain-derived neurotrophic factor</span></em>. <b>Conclusion: </b>ADSC can be an alternative source in cell-based therapy for neurodegene</font></span></span> Selegiline, Neurotrophin, Transdifferentiation 113 121 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-329&slc_lang=en&sid=1 Alireza Abdanipour علیرضا عبدانی پور No Taki Tiraihi تقی طریحی takialtr@modares.ac.ir Yes AliReza Delshad علیرضا دلشاد No