Iranian Biomedical Journal
مجله بیومدیکال ایران
IBJ
Basic Sciences
http://ibj.pasteur.ac.ir
1
admin
1028-852X
2008-823X
-
10.61186/ibj
-
8888
-
en
jalali
1390
7
1
gregorian
2011
10
1
15
4
online
1
fulltext
en
مطالعه مورفومتریک سلولهای PSA-NCAM مثبت در هیپوکامپ نوزادان رت بدنبال القاء تشنج در دوره بارداری
Morphometrical Study of Polysialylated Neural Cell Adhesion Molecule Positive Cells in Rat Pups Hippocampus Following Induction of Seizure during Pregnancy
Related Fields
Related Fields
مقاله کامل
Full Length/Original Article
Background:The polysialylated neural cell adhesion molecule (PSA-NCAM) is expressed in developing brain. Fetal brain damage is caused by different conditions such as seizure and hypoxia. The present study was designed to investigate the effect of maternal seizures on the number of PSA-NCAM positive cells in pup's hippocampus. Methods: Female Wistar rats were divided into four groups: (a) kindled rats which received PTZ (40 mg/kg, i.p.) during pregnancy from embryonic day 14-19 (E14-E19) every 48 h, (b) kindled rats which did not receive PTZ during pregnancy, (c) non-kindle, pregnant rats which received PTZ injection (40 mg/kg, i.p.) during pregnancy from E14 to E19 every 48 h, and (d) non-kindle, pregnant rats which received injection with an equal volume of normal saline as sham controls. At postnatal day 14 (PD14), rat pups were perfused, and their brain were fixed, embedded and coronal sections stained by immunohistochemistry method. The number of PSA-NCAM positive cells per unit area in the pup's hippocampus was counted. Results: The number of PSA-NCAM positive cells in the CA1, CA3, and DG fields of pup's hippocampus, which was obtained from mothers who experienced PTZ injection during pregnancy, was decreased approximately 2.6 (P = 0.001), 2 (P = 0.001), and 2.1 (P = 0.001) times compared with non-PTZ treated maternal groups, respectively. Conclusions: Our study showed that maternal seizures reduced the number of neurons and also PSA-NCAM positive cells per unit area in the offspring hippocampus that it may cause impairment in hippocampal functions.
Epilepsy, Polysialylated neural cell adhesion molecule (PSA-NCAM), Hippocampus, Seizures
157
163
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-321&slc_lang=en&sid=1
Ali Akbar
Rajabzadeh
علی اکبر
رجب زاده
100319475328460056614
100319475328460056614
No
Dept. of Anatomical Sciences and Cellular Biology, School of Medicine, Mashhad University
دانشگاه علوم پزشکی مشهد، دانشکده پزشکی، گروه علوم تشریحی و بیولوژی سلولی
Ali Reza
Ebrahimzadeh Bideskan
علیرضا
ابراهیم زاده بیدسکان
ebrahimzadehba@yahoo.com
100319475328460056615
100319475328460056615
Yes
Dept. of Anatomical Sciences and Cellular Biology, School of Medicine, Mashhad University
دانشگاه علوم پزشکی مشهد، دانشکده پزشکی، گروه علوم تشریحی و بیولوژی سلولی
Hossein
Haghir
حسین
حقیر
100319475328460056616
100319475328460056616
No
Dept. of Anatomical Sciences and Cellular Biology, School of Medicine, Mashhad University
دانشگاه علوم پزشکی مشهد، دانشکده پزشکی، گروه علوم تشریحی و بیولوژی سلولی
Ali Reza
Fazel
علیرضا
فاضل
100319475328460056617
100319475328460056617
No
Dept. of Anatomical Sciences and Cellular Biology, School of Medicine, Mashhad University
دانشگاه علوم پزشکی مشهد، دانشکده پزشکی، گروه علوم تشریحی و بیولوژی سلولی