en مطالعه مورفومتریک سلولهای PSA-NCAM مثبت در هیپوکامپ نوزادان رت بدنبال القاء تشنج در دوره بارداری Related Fields Related Fields مقاله کامل Full Length/Original Article Background:The polysialylated neural cell adhesion molecule (PSA-NCAM) is expressed in developing brain. Fetal brain damage is caused by different conditions such as seizure and hypoxia. The present study was designed to investigate the effect of maternal seizures on the number of PSA-NCAM positive cells in pup's hippocampus. Methods: Female Wistar rats were divided into four groups: (a) kindled rats which received PTZ (40 mg/kg, i.p.) during pregnancy from embryonic day 14-19 (E14-E19) every 48 h, (b) kindled rats which did not receive PTZ during pregnancy, (c) non-kindle, pregnant rats which received PTZ injection (40 mg/kg, i.p.) during pregnancy from E14 to E19 every 48 h, and (d) non-kindle, pregnant rats which received injection with an equal volume of normal saline as sham controls. At postnatal day 14 (PD14), rat pups were perfused, and their brain were fixed, embedded and coronal sections stained by immunohistochemistry method. The number of PSA-NCAM positive cells per unit area in the pup's hippocampus was counted. Results: The number of PSA-NCAM positive cells in the CA1, CA3, and DG fields of pup's hippocampus, which was obtained from mothers who experienced PTZ injection during pregnancy, was decreased approximately 2.6 (P = 0.001), 2 (P = 0.001), and 2.1 (P = 0.001) times compared with non-PTZ treated maternal groups, respectively. Conclusions: Our study showed that maternal seizures reduced the number of neurons and also PSA-NCAM positive cells per unit area in the offspring hippocampus that it may cause impairment in hippocampal functions. Epilepsy, Polysialylated neural cell adhesion molecule (PSA-NCAM), Hippocampus, Seizures 157 163 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-321&slc_lang=en&sid=1 Ali Akbar Rajabzadeh علی اکبر رجب زاده No Dept. of Anatomical Sciences and Cellular Biology, School of Medicine, Mashhad University دانشگاه علوم پزشکی مشهد، دانشکده پزشکی، گروه علوم تشریحی و بیولوژی سلولی Ali Reza Ebrahimzadeh Bideskan علیرضا ابراهیم زاده بیدسکان ebrahimzadehba@yahoo.com Yes Dept. of Anatomical Sciences and Cellular Biology, School of Medicine, Mashhad University دانشگاه علوم پزشکی مشهد، دانشکده پزشکی، گروه علوم تشریحی و بیولوژی سلولی Hossein Haghir حسین حقیر No Dept. of Anatomical Sciences and Cellular Biology, School of Medicine, Mashhad University دانشگاه علوم پزشکی مشهد، دانشکده پزشکی، گروه علوم تشریحی و بیولوژی سلولی Ali Reza Fazel علیرضا فاضل No Dept. of Anatomical Sciences and Cellular Biology, School of Medicine, Mashhad University دانشگاه علوم پزشکی مشهد، دانشکده پزشکی، گروه علوم تشریحی و بیولوژی سلولی