en توزیع سلول‌های ایمنی خون محیطی در اشخاص پاسخ‌دهنده به واکسن هپاتیت B مقاله کامل Full Length/Original Article <font size="2"><font face="times new roman,times,serif"><span new="" roman="" style="FONT-FAMILY: " times=""><font size="2">Unresponsiveness to hepatitis B surface antigen (HBsAg) has been shown to be associated with dysfunction of the presenting cells (APC) and defect in the specific B-lymphocyte and/or T-lymphocyte repertoires.<span style="mso-spacerun: yes">&nbsp;&nbsp; </span>Direct determination of the frequency of specific T-lymphocytes together with complementary</font> <font size="2">analysis of the naive circulating immune cells could provide valuable information about the cellular basis of unresponsiveness to HBsAg.<span style="mso-spacerun: yes">&nbsp;&nbsp; </span>In this study, </font></span><span new="" roman="" style="FONT-FAMILY: " times=""><font size="2">t</font></span><span new="" roman="" style="FONT-FAMILY: " times=""><font size="2">he phenotypic characteristics of peripheral blood mononuclear cells (PBMC) from healthy adult high-responders</font> <font size="2">(n = 19), intermediate-responders (n = 11), low-responders (n = 9) and non-responders<span style="mso-spacerun: yes">&nbsp; </span>(n = 6) to recombinant hepatitis B vaccine were investigated and compared. The proportions of circulating B-lymphocytes (CD19</font></span><font size="2">^{<span new="" roman="" style="FONT-FAMILY: " times="">+</span>}<span new="" roman="" style="FONT-FAMILY: " times=""> cells), T-lymphocytes (CD3</span>^{<span new="" roman="" style="FONT-FAMILY: " times="">+</span>}<span new="" roman="" style="FONT-FAMILY: " times=""> cells) and monocytes (CD14</span>^{<span new="" roman="" style="FONT-FAMILY: " times="">+</span>}</font><span new="" roman="" style="FONT-FAMILY: " times=""><font size="2">)<span style="mso-spacerun: yes">&nbsp; </span>were similar</font> <font size="2">in all groups<span style="mso-spacerun: yes">&nbsp; </span>of<span style="mso-spacerun: yes">&nbsp; </span>responder individuals (14%,<span style="mso-spacerun: yes">&nbsp; </span>55-60%<span style="mso-spacerun: yes">&nbsp; </span>and<span style="mso-spacerun: yes">&nbsp; </span>11-13%, respectively) compared to non-responders (16%, 64% and<span style="mso-spacerun: yes">&nbsp; </span>9%,<span style="mso-spacerun: yes">&nbsp; </span>respectively). These results suggest that the cellular basis for the lack of response to HBsAg is not associated to a generalized deficiency of immune cells in the non-responder subjects,</font> <font size="2">rather it may reflect a defect in HBsAg-specific B or T cells.</font></span></font></font> HBs antigen, anti-HBs antibody, hepatitis B vaccine, B-lymphocyte, T-lymphocyte 1 5 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-311&slc_lang=en&sid=1 Mohammad Ali Shokrgozar محمد علی شکرگزار No Hamid Mahmoodzadeh-Niknam حمید محمودزاده نیکنام No Fazel Shokri فاضل شکری fazshok@yahoo.com Yes