en Related Fields Related Fields مقاله کامل Full Length/Original Article <div style="text-align: justify;"><span style="font-size:14px;"><span style="font-family:Times New Roman;"><span style="line-height:normal"><b><span style="color:black">Background: </span></b><span style="color:black">Research into antimicrobial peptides (AMPs) and other novel antimicrobial strategies continues to be a focus of the scientific community to address the growing challenges posed by antibiotic resistance. </span>The aim of this study was the specific bioinformatic design of a novel hybrid AMP that combines the potent antibacterial properties of Temporins and Brevinin while minimizing its cytotoxic effects. <span style="color:black"></span></span><br> <span style="line-height:normal"><b><span style="color:black">Methods</span></b><span style="color:black">: The N-terminal of Brevinin peptides and the C-terminal of Temporin peptide were selected. To optimize the peptide, we replaced the amino acids at positions 1, 3, and 7 with glycine, lysine, and lysine, respectively. Various approaches were used to assess physicochemical characteristics, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), cytotoxicity, apoptosis, hemolytic activity, and </span>reactive oxygen species<b> </b><span style="color:black">generation.</span></span><br> <span style="line-height:normal"><b><span style="color:black">Results: </span></b><span style="color:black">The novel peptide had 57% hydrophobicity and four positive electric charges due to its lysine amino acid composition. The synthesized peptide exhibited MIC and MBC values of 80 &micro;M against <i>Pseudomonas aeruginosa</i>. According to the MTT assay, the peptide showed neither cytotoxic nor hemolytic effects on human cells. </span>The results of electron microscopy showed that this peptide can disrupt the integrity of <i>P. aeruginosa</i> membrane cells and induce morphological changes in their surface structure.<span style="color:black"></span></span><br> <b><span style="line-height:115%"><span style="color:black">Conclusion: </span></span></b><span style="line-height:115%"><span style="color:black">This research suggests that the synthetic peptide could serve as a promising potential therapeutic agent to combat antibiotic-resistant strains of <i>P. aeruginosa</i>. </span></span></span></span></div> Antibacterial peptides, Pseudomonas aeruginosa, Temporins, Brevinins 81 89 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1944-2&slc_lang=en&sid=1 Armineh Azadian hosseini@yahoo.com 0009-0000-4845-7868 No 1Department of Biology ،Faculty of Basic Sciences, Islamic Azad University, Science and Research Branch, Tehran, Iran Hamideh Mahmoodzadeh Hosseini hosseini361@yahoo.com 0000-0002-3987-0164 Yes Applied Microbiology Research Center, Biomedicine Technologies Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran Seyed Ali Mirhosseini hani@yahoo.com 0000-0002-4065-7213 No Applied Microbiology Research Center, Biomedicine Technologies Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran Soghra Khani skhani_22@yahoo.com 0000-0002-2189-6374 No Department of Biochemistry, Pasteur Institute of Iran, Tehran, Iran Jafar Amani khan_22@yahoo.com 0000-0002-5155-4738 No Applied Microbiology Research Center, Biomedicine Technologies Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran