Iranian Biomedical Journal
مجله بیومدیکال ایران
IBJ
Basic Sciences
http://ibj.pasteur.ac.ir
1
admin
1028-852X
2008-823X
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10.61186/ibj
-
8888
-
en
jalali
1381
12
1
gregorian
2003
3
1
7
2
online
1
fulltext
en
شناسایی گونههای لیشمانیا و سویههای لیشمانیا ماژور در مناطق مختلف اندمیک لیشمانیوز پوستی در ایران
Characterization of Leishmania Species and L. major Strains in Different Endemic Areas of Cutaneous Leishmaniasis in Iran
مقاله کامل
Full Length/Original Article
<font face="times new roman,times,serif">Both zoonotic and anthroponotic cutaneous leishmaniasis (CL) caused by L. major and L. tropica, respectively, are endemic in different parts of Iran. This study was performed to investigate the new changes in epidemiological pattern of CL, and to identify the species of Leishmania and the strains of L. major isolated from different endemic areas of Iran. Seventy-two isolates from 245 samples collected from different endemic areas of Iran were characterized by monoclonal antibodies (mAb) specific for L. major, L. tropica, and L. infantum. Flow cytometry, isoenzyme analysis and PCR amplification were used for identification. Isoenzyme analysis was carried out by PAGE and cellulose acetate. Eight enzymes including malate dehydrogenase (MDH), malic enzyme (ME), glucose 6-phosphate dehydrogenase (G6PD), glucose phosphate isomerase (GPI), nucleoside hydrolase inosine (NHi), nucleoside hydrolase deoxy inosine (NHd), superoxide dismutase (SOD) and 6-phosphogluconate dehydrogenase (6PGD) were used for isoenzyme analysis. PCR assay was developed using specific primers against kinetoplast DNA. The isolates were compared with reference strains (RS). Data obtained from different methods showed 45 out of 72 isolates were similar to RS of L. major and 22 similar to L. tropica, and also five samples were identified as Crithidia. Isoenzyme migration pattern of L. major isolates was indistinguishable from each other in six enzymatic systems but differences were observed in profile of two enzymes of SOD and MDH. The data indicate that L. major species are dominant in the studied endemic areas, and different strains of L. major are present in different geographic areas of Iran. Moreover, it seems that enzymatic system is more useful approach than others for characterization of Leishmania species and L. major strains </font>
Leishmania species, monoclonal antibodies (mAb), isoenzyme, PCR
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http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-271&slc_lang=en&sid=1
Mahnaz
Tashakori
مهناز
تشکری
100319475328460057674
100319475328460057674
No
Soheila
Ajdary
سهیلا
اژدری
100319475328460057675
100319475328460057675
No
Amina
Kariminia
آمینا
کریمی نیا
100319475328460057676
100319475328460057676
No
Fereidoun
Mahboudi
فریدون
مهبودی
100319475328460057677
100319475328460057677
No
Mohammad Hossein
Alimohammadian
محمدحسین
علیمحمدیان
mhalimoh@institute.pasteur.ac.ir
100319475328460057678
100319475328460057678
Yes