Iranian Biomedical Journal
مجله بیومدیکال ایران
IBJ
Basic Sciences
http://ibj.pasteur.ac.ir
1
admin
1028-852X
2008-823X
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10.61882/ibj
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8888
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en
jalali
1404
10
1
gregorian
2026
1
1
30
1
online
1
fulltext
en
Effect of HIF-1α and LDHA Blockade on Gene Expression of Tumor Immune Evasion in Myeloid Leukemia Cell Lines
Cancer Biology
Cancer Biology
مقاله کامل
Full Length/Original Article
<div style="text-align: justify;"><span style="font-family:Times New Roman;"><span style="font-size:11pt"><span style="line-height:normal"><b><span style="font-size:10.5pt">Background:</span></b> <span style="font-size:10.5pt">Tumor cells utilize metabolic reprogramming to obtain the energy required for rapid proliferation. In this study, we blocked two key factors, hypoxia-inducible factor 1-alpha (HIF-1α) and lactate dehydrogenase (LDHA), in the metabolic pathway in acute myeloid leukemia (AML) cell lines to evaluate their effect on the expression of genes involved in tumor <span style="color:black">evasion.</span></span><span style="font-size:10.5pt"></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><b><span style="font-size:10.5pt">Methods</span></b><span style="font-size:10.5pt">: </span><span style="font-size:10.5pt"><span style="color:black">Silibinin and sodium oxamate, as <i>HIF-1α</i> and <i>LDHA</i> blockers, respectively, were used to treat </span>K-562 and HL-60 <span style="color:black">cells. The MTT assay was used </span>to assess cell viability, and quantitative reverse transcription<b> </b>PCR was used to measure the mRNA expression levels of <i>PD-L1</i>, <i>CD47</i>, and <i>CD155</i>.</span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><b><span style="font-size:10.5pt">Results:</span></b><i> </i><span style="font-size:10.5pt">Both K-562 and HL-60 cells showed a significant decrease in <i>CD47</i> expression. Additionally, HL-60 cells indicated a significant suppression of <i>CD155</i> expression. In contrast, <i>PD-L1</i> expression remained unchanged after treatment.</span><b><i><span style="font-size:10.5pt"></span></i></b></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><b><span style="font-size:10.5pt">Conclusion</span></b><span style="font-size:10.5pt">: Our findings suggest that blocking signaling pathways involved in metabolic reprogramming of cancer cells could be a promising approach for modulating the expression of certain immune checkpoint ligands, warranting further investigation. </span></span></span></span><br>
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Acute myeloid leukemia, Hypoxia, Lactates, Warburg effect
91
97
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-6584-1&slc_lang=en&sid=1
Tamana
Eskandari
tamana.eskandari@gmail.com