Iranian Biomedical Journal

en Transcriptional Control of PI3K/AKT/mTOR by piRNA-651 in Breast Carcinoma Cancer Biology Cancer Biology مقاله کامل Full Length/Original Article <div style="text-align: justify;">Background: Breast cancer (BC) is among the most prevalent malignancies in women worldwide, yet early diagnosis is associated with a high survival rate. The proliferation of BC is linked to the overexpression of genes within the PI3K/AKT/mTOR signaling pathway. piR-651 has been reported to be effective in the proliferation and metastasis of BC. This research sought to evaluate the impact of piR-651 inhibition on the PI3K/AKT/mTOR pathway in HUVEC, MCF-7, and MDA-MB-231 cells. Methods: Anti-piR-651 and non-target sequences were introduced into HUVEC, MCF-7, and MDA-MB-231 BC cells by lipofectamine transfection. After 48 hours, total RNA was extracted, and qRT-PCR assessed the gene expression of PI3K, AKT, and mTOR. Results: Anti-piR-651 treatment significantly increased PI3K, AKT, and mTOR gene expression in HUVECs (p < 0.001). In contrast, PI3K and mTOR expression decreased in MCF-7 and MDA-MB-231 cells (p < 0.001), while AKT expression remained unchanged in MDA-MB-231 cells (p > 0.05). Correlations between these genes varied by cell type, with significant associations observed at p < 0.05 or p < 0.01, depending on the group. Conclusion: piR-651 inhibition causes AKT to behave independently of PI3K and mTOR, particularly in MCF-7 cells, suggesting limited gene therapy potential for estrogen receptor-positive BC. Preliminary data indicate that piR-651 inhibition may reduce BC cell proliferation through effects on PI3K and mTOR. </div> Epigenetics, Piwi-interacting RNA, Triple negative breast neoplasms 397 404 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-6451-1&slc_lang=en&sid=1 Sena Köstü senakostu5@gmail.com 0009-0005-8485-7857 No Kırklareli University, Faculty of Medicine, Kırklareli, TURKEY Baturalp Bakirci bakircibaturalp@gmail.com 0009-0006-9985-0831 No Kırklareli University, Faculty of Medicine, Kırklareli, TURKEY Ece Bilge Şimşek ece.bilge.simsek@gmail.com 0009-0002-1318-7540 No Kırklareli University, Faculty of Medicine, Kırklareli, TURKEY Defne Dumanli defne2003.dd@gmail.com 0009-0002-6267-8872 No Kırklareli University, Faculty of Medicine, Kırklareli, TURKEY Ahmetcan Çelik canahmetcelik@gmail.com 0009-0002-4678-3445 No Kırklareli University, Faculty of Medicine, Kırklareli, TURKEY Alara Kaya kaya.alara123@gmail.com 0009-0007-7560-2750 No Kırklareli University, Faculty of Medicine, Kırklareli, TURKEY Damla Kolcuoğlu damlakolcuoglu@klu.edu.tr 0009-0009-8682-5604 No Kırklareli University, Faculty of Medicine, Department of Medical Biology, Kırklareli, TURKEY Ertuğrul Çolak ecolak@ogu.edu.tr 0000-0003-3251-1043 No Eskişehir Osmangazi University, Faculty of Medicine, Department of Biostatistics, Eskişehir, TURKEY Çağrı Öner cagrioner@klu.edu.tr 0000-0003-3771-3277 Yes Kırklareli University, Faculty of Medicine, Department of Medical Biology, Kırklareli, TURKEY