en Related Fields Related Fields سیتماتیک ریویو Systematic Review <div style="text-align: justify;"><span style="font-family:Times New Roman;"><span style="font-size:10.5pt"><span style="line-height:115%"><span style="color:black">Insulin therapy is essential for regulating glucose levels in diabetic patients. However, subcutaneous insulin injection, despite being a standard procedure, is invasive and often painful and can lead to complications such as skin damage and infections. These issues often result in poor patient compliance and inadequate glycemic control. Recently, transdermal insulin administration has been explored as an alternative to subcutaneous methods; however, no universally accepted protocol has been developed for its use. This systematic review aimed to evaluate the effect of iontophoresis on transdermal insulin administration in laboratory animals. Using the PICO search strategy and in accordance with PRISMA guidelines, relevant articles published from January 1980 to May 2025 were retrieved from Scopus, PubMed, ISI Web of Science, PEDro, Science Direct, and Google Scholar databases. The findings from these studies suggest that combining iontophoresis with physical enhancers of skin penetration can effectively regulate blood glucose levels while minimizing the risk of hypoglycemia. </span></span></span></span></div> Diabetes mellitus, Laboratory animals, Skin 189 205 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-6321-1&slc_lang=en&sid=1 Zeinab Ahmadpour Emshi zeinab.ahmadpour1372@gmail.com 0000-0002-2241-455X No Student Research Committee, Ph.D. Candidate of Physiotherapy, Department of Physiotherapy, School of Rehabilitation, Shahid Beheshti University of Medical Sciences, Tehran, Iran Mohammad Mohsen Roostayi mohsen42@yahoo.com 0000-0002-5866-6505 No Physiotherapy Research Center, Department of Physiotherapy, School of Rehabilitation, Shahid Beheshti Medical University, Tehran, Iran. Aliyeh Daryabor aliyehdaryabor@gmail.com 0000-0002-0652-6025 No Physiotherapy Research Center, Department of Physiotherapy, School of Rehabilitation, Shahid Beheshti University of Medical Science, Iran, Tehran. Maryam Niajalili maryam_niajalili@yahoo.com 0000-0001-7549-6969 No Student Research Committee, Ph.D. Candidate of Physiotherapy, Department of Physiotherapy, School of Rehabilitation, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Sedigheh Sadat Naimi naimi.se@gmail.com 0000-0001-7772-5737 Yes Physiotherapy Research Center, Department of Physiotherapy, School of Rehabilitation, Shahid Beheshti Medical University, Tehran, Iran