Iranian Biomedical Journal

en Development of Experimental Platforms to Assess Helicobacter pylori HopQ Interaction with Host CEACAM Molecules Medical Biotechnology Medical Biotechnology مقاله کامل Full Length/Original Article <div>Background: Helicobacter pylori is an extracellular bacterium responsible for various gastrointestinal diseases, such as peptic ulcers and gastric cancer. It uses multiple mechanisms to colonize the harsh, acidic environment of the stomach and establish its pathogenic processes, mostly through CagA translocation. While cell surface integrin molecules were previously believed to be the main mediators anchoring H. pylori and facilitating this process, recent studies highlight the critical role of the interaction between the bacterial adhesin Helicobacter pylori outer membrane protein Q (HopQ) and host carcinoembryonic antigen-related cell adhesion molecules (CEACAMs) in CagA translocation and subsequent pathogenic signaling. Methods: Recombinant proteins, including HopQ, HopQ-GFP (green fluorescent protein), HopQ-HRP (horseradish peroxidase), and recombinant N-terminal domain of human CEACAM1 (C1ND), were produced via gene cloning, expression, and purification techniques. Ligand-receptor interactions were evaluated using FACS analysis along with antigen- and cell-based ELISA assays. Results: In this study, we have developed antigen and cell-based platforms using recombinant fusion proteins (HopQ-GFP and HopQ-HRP) that effectively interact with recombinant C1ND, as well as various CEACAM molecules expressed on gastric cell lines (MKN45 and AGS). Conclusion: These assay platforms enable detailed investigation of the HopQ-CEACAM interaction and supports high-throughput screening of inhibitors, facilitating the identification of potential drugs or vaccine candidates targeting H. pylori infection. </div> Adhesin, Enzyme-linked immunosorbent assay, Ligands, Receptor 138 148 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-670-10&slc_lang=en&sid=1 Nazanin Shans nazaninshans@gmail.com 0009-0006-6394-4879 No HPGC Research Group, Department of Medical Biotechnology, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran Maryam Esmaeili m_esmaily2007@yahoo.com 0000-0002-6203-4332 No HPGC Research Group, Department of Medical Biotechnology, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran Kimia Abraheh abraheh.kimia@gmail.com 0009-0003-4562-313X No HPGC Research Group, Department of Medical Biotechnology, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran Niloofar Asadi Hanjani asadi.niloofar75@gmail.com 0009-0000-6793-6477 No HPGC Research Group, Department of Medical Biotechnology, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran Maedeh Farrokhi maede_farokhi@yahoo.com 0009-0005-9224-4591 No HPGC Research Group, Department of Medical Biotechnology, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran Negar Sardarpour negar_sardarpour@yahoo.com 0000-0003-2646-7648 No HPGC Research Group, Department of Medical Biotechnology, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran Yeganeh Talebkhan ytgaroosi@gmail.com 0000-0003-1988-4419 No HPGC Research Group, Department of Medical Biotechnology, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran Fatemeh Kazemi-Lomedasht fatemeh1044@yahoo.com 0000-0002-5832-1822 No HPGC Research Group, Department of Medical Biotechnology, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran Esmat Mirabzadeh mirabzadehe@yahoo.com 0000-0003-3636-0780 No HPGC Research Group, Department of Medical Biotechnology, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran Marjan Mohammadi marjan.mohammadi2010@gmail.com 0000-0003-4970-3455 Yes HPGC Research Group, Department of Medical Biotechnology, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran