Iranian Biomedical Journal

en Upregulation of CircCSPP1, CircNRIP1, and CircSMAD2 in Breast Cancer and Their Potential as Diagnostic Biomarkers Cancer Biology Cancer Biology مقاله کامل Full Length/Original Article <div style="text-align: justify;">Background: Identifying novel diagnostic biomarkers with stable structures, such as circulating circular RNAs (circRNAs) can improve the early detection and management of BC. Herein, we conducted this study to analyze the expression profile of four circRNAs, including circCSPP1 (hsa_circ_0001806), circNRIP1 (hsa_circ_0004771), circSMAD2 (hsa_circ_0000847), and circFOXP1 (hsa_circ_0008234) in BC. Methods: Tumor tissues and adjacent normal tissues were obtained from patients with sporadic breast cancer (BC). Divergent primers were designed to amplify the target transcripts using quantitative real-time PCR. The expression profiles of circRNAs were analyzed in tumor and adjacent normal tissues. Sanger sequencing was performed to confirm the back-splicing junctions of circRNAs. ROC curves were generated to assess the potential of the mentioned RNAs as diagnostic biomarkers. Results: We observed a significant upregulation of circCSPP1, circNRIP1, and circSMAD2 in tumor tissue compared to adjacent normal tissues. Among them, circCSPP1 was the most highly upregulated one in tumor samples from 39 patients. Expression of circCSPP1 was significantly higher in estrogen receptor (ER)-negative, progesterone receptor (PR)-negative, human epidermal growth factor receptor-2 (HER2)-negative, and triple-negatives compared to ER-positive, PR-positive, HER2-positive, and non-triple-negative ones. Expression of circNRIP1 was also significantly elevated in the ER-negative and the triple-negative subgroups. These three circRNAs also displayed a desirable potential as diagnostic biomarkers. Conclusion: This is the first paper that reports the upregulation of circCSPP1 and circSMAD2 in BC and upregulation of circCSPP1 and circNRIP1 in the triple-negative subgroup. Our findings suggest that circCSPP1, circNRIP1, and circSMAD2 may serve as promising diagnostic biomarkers for BC. Identifying the downstream pathways regulated by these circRNAs could lead to the discovery of new therapeutic targets.</div> Breast neoplasms, Circular RNA, Triple negative breast neoplasms, Tumor biomarkers 159 166 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-6088-1&slc_lang=en&sid=1 Mahsa Keshavarz-Fathi Mahsa.keshavarz20@gmail.com 0000-0001-8226-5878 No School of Medicine, Tehran University of Medical Sciences, Tehran, Iran Farzaneh Darbeheshti b.darbeheshti70@gmail.com 0000-0002-4957-9004 No Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran Rangarirai Makuku rangarirai.makuku@gmail.com 0000-0003-3305-2053 No School of Medicine, Tehran University of Medical Sciences, Tehran, Iran Parmida Sadat Pezeshki pezeshkiparmida@gmail.com 0000-0003-2817-4734 No Cancer Immunology Project (CIP), Universal Scientific Education and Research Network (USERN), Tehran, Iran Homa Seyedmirzaei homa_sdmr@yahoo.com 0000-0001-7799-9332 No School of Medicine, Tehran University of Medical Sciences, Tehran, Iran Yaser Mansoori fums.mansoori@gmail.com 0000-0002-9101-5552 No Noncommunicable Disease Research Center, Fasa University of Medical Sciences, Fasa, Iran Ali Mohammad Mosadeghrad mosadeghrad@tums.ac.ir 0000-0002-7955-6292 No School of Public Health, Health Information Management Research Center, Tehran University of Medical Sciences, Tehran, Iran Nima Rezaei rezaei_nima@yahoo.com 0000-0002-3836-1827 Yes Research Center for Immunodeficiencies, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran