en Related Fields Related Fields مقاله کامل Full Length/Original Article <div style="text-align: justify;"><span style="font-family:Times New Roman;"><span style="font-size:11pt"><span style="line-height:normal"><b><span style="font-size:10.5pt">Background:</span></b><span style="font-size:10.5pt"> Chemoresistance, the primary cause of disease relapse and treatment failure, poses a significant challenge in the treatment of colorectal cancer (CRC). Understanding the molecular mechanisms that underlie the pathogenesis and chemoresistance of colorectal tumor cells, as well as identifying novel therapeutic strategies, would be crucial. This study aimed to evaluate the role of <i>hsa_Circ_0001821</i> in response to 5-fluorouracil (5-FU) in CRC, a topic that has not been examined to date.</span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><b><span style="font-size:10.5pt">Methods:</span></b><span style="font-size:10.5pt"> The current study investigated the effect of <i>hsa_Circ_0001821</i> suppression using interfering RNAs on the response of colorectal tumor cells to 5-FU. The expression levels of <i>hsa_Circ_0001821</i>, <i>hsa-miR-203a-3p</i>, <i>BAX</i>, <i>BCL-2</i>, and <i>FGF-2</i> were determined via quantitative RT-PCR. Cell survival, migration rate, and apoptosis induction of colorectal tumor cells subjected to 5-FU treatment were assessed using the MTT test, scratch assay, and flow cytometry analysis, respectively.</span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><b><span style="font-size:10.5pt">Results:</span></b><span style="font-size:10.5pt"> Knockdown of <i>hsa_Circ_0001821</i> with siRNA increased the expression level of <i>hsa-miR-203a-3p</i> and decreased the expression level of <i>FGF-2</i>. Additionally, the knockdown of <i>hsa_Circ_0001821</i> enhanced the sensitivity of colorectal tumor cells to 5-FU. This circRNA significantly affected the viability, apoptosis, and migration of tumor cells.</span></span></span><br> <b><span style="font-size:10.5pt"><span style="line-height:115%">Conclusion:</span></span></b><span style="font-size:10.5pt"><span style="line-height:115%"> Our study reveals the potential role of <i>hsa_Circ_0001821</i> in controlling the tumor cell viability and response to 5-FU by targeting the <i>hsa-miR-203a-3p</i>/<i>FGF-2 </i>axis. These findings enhance our understanding of the molecular mechanisms that influence chemotherapy response in CRC, paving the way for the identification of more effective treatments for this disease</span></span><span style="font-size:10.5pt"><span style="line-height:115%">.</span></span> </span></div> Colorectal neoplasm, Circ_0001821, 5-fluorouracil, miR-203a-3p, FGF-2 82 89 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-2785-2&slc_lang=en&sid=1 Pejman Molaei safsar.h@gmail.com 0000-0002-7348-9041 No Department of molecular medicine, School of Advanced Medical Sciences and Technologies, Hamadan University of Medical Sciences, Hamadan, Iran Ali Mahdavinezhad safhar.h@gmail.com 0000-0002-1314-8229 No Department of molecular medicine, School of Advanced Medical Sciences and Technologies, Hamadan University of Medical Sciences, Hamadan, Iran Rezvan Najafi sashar.h@gmail.com 0000-0003-1326-1288 No Department of molecular medicine, School of Advanced Medical Sciences and Technologies, Hamadan University of Medical Sciences, Hamadan, Iran Mehrdad Hashemi safsha.h@gmail.com 0000-0003-0627-6991 No Department of Genetics, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran Leili Tapak afshar.h@gmail.com 0000-0002-4378-3143 No Modeling of Noncommunicable Diseases Research Center, Institute of Health Sciences and Technologies, Hamadan University of Medical Sciences, Hamadan, Iran Saeid Afshar safshar.h@gmail.com 0000-0001-5136-4259 Yes Department of Biostatistics, School of Public Health, Hamadan University of Medical Sciences, Hamadan, Iran