Iranian Biomedical Journal

en Unraveling Leishmania major Metacyclogenesis: A Comprehensive Analysis of Transcriptomic and Metabolomic Profiles Related Fields Related Fields مقاله کامل Full Length/Original Article <div style="text-align: justify;">Background: Metacyclogenesis is a critical developmental process in the life cycle of Leishmania parasites, particularly in their transition from non-infective procyclic to infective metacyclic promastigotes. This transformation is closely linked to the metabolic adaptation of the parasite, optimizing its survival and study, we integrated metabolomics and transcriptomics data to gain deeper molecular mechanisms of Leishmania major metacyclogenesis. Methods: The metabolic profiles of procyclic and metacyclic promastigotes were first identified using ¹H-NMR spectroscopy. Multivariate statistical analysis conducted to distinguish different metabolites between the two forms. Metabolic pathway analysis was performed using the KEGG database to identify the metabolic pathways that significantly altered and overrepresented in the metabolomic profile. Finally, the differential gene expression and pathway enrichment analyses were conducted on transcriptomic data retrieved from public repositories. Results: Multivariate statistical analysis revealed that 44 metabolites and ten pathways were significantly different between the two forms. Transcriptome genes during metacyclogenesis. These genes underwent GO and KEGG pathway analyses, revealing upregulated GO categories in the metacyclic phase, including protein phosphorylation, ion transport, signal transduction, and phosphorylation reactions, as well as several downregulated GO categories. Integrating metabolomic and transcriptomic data demonstrated seven significantly different KEGG pathways between procyclic and metacyclic forms, including fructose and mannose, galactose, ascorbate and aldarate, arginine and proline, histidine, inositol phosphate, and pyruvate metabolism. Conclusion: Our findings suggest distinct metabolic profiles and changes in gene expression associated with the transition from procyclic to metacyclic promastigotes. By integrating diverse omics data, we could identify more reliable altered pathways and biomarkers.</div> Leishmania, Transcriptomics, Metabolomics, Procyclic, Metacyclic, Metacyclogenesis 68 81 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-521-1&slc_lang=en&sid=1 Mansour Aminzadeh samin415@gmail.com 0009-0006-7982-0360 No Metabolomics Lab, Department of Biochemistry, Pasteur Institute of Iran, Tehran, Iran Fariborz Bahrami fariborz.bahrami@mail.mcgill.ca 0000-0001-9561-415X No Department of Immunology, Pasteur Institute of Iran,Tehran, Iran Zeynab Piryaei ajdsoh@pasteur.ac.ir 0000-0003-4767-6676 No Department of Bioinformatics, Kish International Campus, University of Tehran, Kish, Iran Mahdi Vasighi Vasighi@gmail.com 0000-0002-3048-4638 No Department of Computer Sciences, Institute for Advanced Studies in Basic Sciences, Zanjan, Iran Zahra Kalantari Kalantary@gmail.com 0009-0006-2856-6497 No Iran Polymer and Petrochemical Institute. Department of Polymerization Engineering, Tehran, Iran Mohammad Arjmand* arjmand2@gmail.com 0000-0001-7825-7883 No Metabolomics Lab, Department of Biochemistry, Pasteur Institute of Iran, Tehran, Iran Soheila Ajdary sohary@yahoo.com 0000-0002-5052-2523 Yes Department of Immunology, Pasteur Institute of Iran, Tehran, Iran