en ارائه روش جدید برای تشخیص آنزیم تباسیلکو دکسترین گلوکوزیل ترانسفراز بر ژل پلی اکریلامید مقاله کامل Full Length/Original Article <font face="times new roman,times,serif">b-cyclodextrin glucosyl transferase (b-CGTase) hydrolyses starch to produce b-cyclodextrin by transglycosylation (cyclization) activity. The conventional method for detection of b-CGTase activity is based on detecting starch hydrolysis by iodine staining. This method reveals all amylolytic enzymes, but can not discriminate them. In the present study, we introduce a new method for specific detection of b-CGTase activity and its specific product i.e. b-cyclodextrin by polyacrylamide gel. In this method, solution containing b-CGTase is subjected to electrophoresis on 10% polyacrylamide gel. Then, the gel is covered with an indicator gel containing phenolphthalein, soluble starch, and agar. After a short incubation, sodium carbonate solution is added and the positive result is indicated by the emergence of a colorless band in the red context of the indicator gel. Since the production of b-cyclodextrin via cyclization is unique to b-CGTase, the emergence of clear bands is indicative of b-CGTase presence. However, in conventional starch-iodine method, all amylolytic enzymes including b-CGTase give positive results. Therefore, for detection of b-CGTase, the phenolphthalein indicator gel method, compared to the starch-iodine method, is more specific </font> Cyclodextrin, Phenolphthalein, PAGE, Cyclodextrin glucosyl transferase (CGTase), Zymogram 87 90 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-212&slc_lang=en&sid=1 Saeed-Reza Pakzad سعیدرضا پاکزاد srpakzad@yahoo.com Yes Soheila Ajdary سهیلا اژدری No Nasrin Moazami نسرین معظمی No Saeed Haghighi سعید حقیقی No