other Related Fields Related Fields مقاله کامل Full Length/Original Article <div style="text-align: justify;"><span style="font-family:Times New Roman;"><span style="font-size:14px;"><strong><span style="color:#0e101a">Introduction: </span></strong><span style="color:#0e101a">Glioblastoma multiforme (GBM) and spinal cord tumors have a high recurrence rate. Recent studies have suggested that Riluzole has anti-tumoral effects. We evaluated the efficacy of combination therapy with Riluzole and temozolomide (TMZ) on glioblastoma stem-like cells (GSCs) and spinal cord tumor stem cells (SCTSCs) in vitro. </span><br> <strong><span style="color:#0e101a">Methods and Materials: </span></strong><span style="color:#0e101a">Cells were extracted from two patients with spinal cord tumors and three patients with GBM. We evaluated the effects of combinatorial and monotherapy with Riluzole and TMZ on cultured stem cells. The MTS, the CytoTox-Fluor&trade; Cytotoxicity, and the Caspase-Glo&reg; 3/7 assays were used to assess cell viability, the protease activity of the dead cells, and early apoptosis, respectively. </span><br> <strong><span style="color:#0e101a">Results:</span></strong><span style="color:#0e101a"> The study included two men with spinal cord tumors and a man and two women with GBM. We found no notable differences between the combination therapy and monodrug therapy of GSCs and SCTSCs in the case of cell viability. However, the apoptosis of GSCs and SCTSCs was significantly induced after 24 h treatment with both monotherapy and combination therapies (<i>p</i> = 0.05). However, it turned to non-significant in GSCs after 48 h. The cytotoxicity of GSCs was significant after 24 h of combination therapy (</span><em><span style="color:#0e101a">p</span></em><span style="color:#0e101a"> = 0.05), while the cytotoxicity of the SCTSCs turned significant after 48 h in both therapies (</span><em><span style="color:#0e101a">p</span></em><span style="color:#0e101a"> = 0.05). </span><br> <strong><span style="line-height:115%"><span style="color:#0e101a">Conclusion and Discussion:</span></span></strong><span style="line-height:115%"><span style="color:#0e101a"> The data from the present study suggest that combination therapy with Riluzole and TMZ may indicate superior anti-tumoral effects on GSCs and SCTSCs compared to monotherapy.</span></span><br> <br> <img alt="" src="./files/site1/images/888.jpg" style="width: 1000px; height: 36px;" ></span></span></div> Glioblastoma, Riluzole, Spinal cord neoplasms, Temozolomide 266 266 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-4696-283&slc_lang=other&sid=1 Maedeh Amiri-Shahri No Fatemeh Rasouli No Neshat Najaf Najafi No Sajad Sahab Negah Yes