other Related Fields Related Fields مقاله کامل Full Length/Original Article <div style="text-align: justify;"><span style="font-family:Times New Roman;"><span style="font-size:14px;"><span style="unicode-bidi:embed"><span style="line-height:115%"><b><span style="line-height:115%"><span style="color:black">Introduction:</span></span></b><span style="line-height:115%"><span style="color:black"> Type 2 diabetes (T2D) is associated with a decrease in antioxidant defense in heart tissue, which leads to increased oxidative stress and cardiovascular complications. This study aimed to determine the effect of eight weeks of high-intensity interval training (HIIT) and mixed probiotic consumption on the amount of catalase (CAT) and malondialdehyde (MDA) in the heart tissue of T2D male rats. </span></span></span></span><br> <span style="unicode-bidi:embed"><span style="line-height:115%"><b><span style="line-height:115%"><span style="color:black">Methods and Materials:</span></span></b><span style="line-height:115%"><span style="color:black"> For this purpose, 25 male Wistar rats (eight weeks old) with an average weight of 200-220 g were divided into five groups, including (1) healthy control, (2) diabetic control, (3) diabetes + HIIT, (4) diabetes + probiotic mixture, and (5) diabetes + HIIT + probiotic mixture. The exercise training protocol was designed five days a week for eight weeks for training groups. T2D was also induced by injecting nicotinamide and streptozotocin (STZ), and rats with blood sugar higher than 200 mg/dL were included in the study as diabetic rats. Also, the probiotic groups received bacterial suspension containing 1010 CFU/ml of three bacteria (<i>Lactobacillus rhamnosus</i> GG, <i>Lactococcus casei</i>, and <i>Lactobacillus roteri</i>) by gavage daily for eight weeks and five days a week. Forty-eight hours after the last intervention, the rats were anesthetized with a combination of xylazine and ketamine, and the heart tissue was collected. ELISA tissue kit was used to measure malondialdehyde<i> </i>(MDA) and CAT. Data analysis was performed using Shapiro-Wilk statistical tests, one-way analysis of variance, and Scheffe&#39;s post hoc test. </span></span></span></span><br> <span style="unicode-bidi:embed"><span style="line-height:115%"><b><span style="line-height:115%"><span style="color:black">Results:</span></span></b><span style="line-height:115%"><span style="color:black"> The results showed that eight weeks of HIIT, along with the consumption of mixed probiotics, significantly increased the CAT level and reduced the MDA level in the heart tissue of STZ-treated diabetic rats (<i>p</i> = 0.05). </span></span></span></span><br> <b><span style="line-height:115%"><span style="color:black">Conclusion and Discussion:</span></span></b><span style="line-height:115%"><span style="color:black"> The findings of the current study demonstrate that combining the two above-mentioned interventions increases the antioxidant defense of cardiomyocytes in T2D, which can be investigated more closely as a therapeutic strategy in diabetic patients.</span></span><br> <br> <img alt="" src="./files/site1/images/888.jpg" style="width: 1000px; height: 36px;" ></span></span></div> High-intensity interval training, Oxidative stress, Probiotics 246 246 http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-4696-265&slc_lang=other&sid=1 Arman Alizadeh No Hossein Shirvani Yes Hamideh Mahmoodzadeh Hosseini No